◆◆◆IE_04B05 detail◆◆◆

Code nameIE_04B05
LocusAT1G06170
TemplateIT_04B05 (HR1254)
Donor VectorpDONR207
Forward primerIPF_04B05 (ggggacaagtttgtacaaaaaagcaggcttcATGGGAGGAGGAGGCATGTTTGAAGAAA)
Reverse primerIPR_04B05 (ggggaccactttgtacaagaaagctgggttATCACTAGAGTAACAGTGATAACCATTATT)
Alignment with template seq.1stアミノ酸配列100%一致2nd-
Alignment with TAIR7CDSアミノ酸配列100%一致-
comment
sequence 
>IE_04B05_1260bp
ATGGGAGGAGGAGGCATGTTTGAAGAAATTGGGTGTTTTGATCCAAATGCTCCGGCAGAAATGACTGCTGAGAGCAGTTTCTCTCCGTCCGAGCCACCAC
CGACGATCACGGTCATCGGTAGCAACAGTAACAGCAACTGCAGTCTAGAAGATCTCTCTGCATTCCACCTCAGCCCACAAGACTCTTCTCTACCTGCCTC
AGCCTCAGCTTATGCTCATCAGCTTCACATTAATGCTACTCCTAATTGTGATCATCAATTCCAGAGTTCAATGCACCAAACCCTCCAAGATCCATCGTAT
GCGCAACAGTCGAATCATTGGGACAATGGTTACCAAGATTTTGTGAATTTGGGTCCTAATCATACCACTCCTGACCTACTCAGTCTCTTGCAATTGCCAA
GATCCTCACTTCCACCTTTCGCAAACCCTAGCATTCAAGACATTATCATGACAACATCTTCCTCTGTCGCCGCCTATGACCCGCTCTTCCATCTGAATTT
TCCTCTGCAGCCTCCTAATGGGAGTTTCATGGGAGTGGATCAAGATCAAACTGAGACTAACCAAGGAGTGAACTTGATGTATGATGAAGAAAACAACAAC
CTCGACGATGGTCTTAACCGTAAAGGCAGAGGATCGAAAAAGCGTAAAATTTTCCCTACAGAACGCGAGAGAAGAGTTCACTTCAAAGACCGGTTTGGTG
ACTTAAAGAATCTAATTCCAAATCCCACCAAGAATGATAGAGCATCAATCGTCGGAGAAGCGATAGATTACATCAAAGAGTTGCTAAGGACGATCGATGA
GTTCAAGCTGCTAGTGGAAAAGAAGAGAGTGAAACAGAGGAACAGAGAAGGAGATGATGTTGTTGATGAGAACTTCAAGGCTCAAAGCGAAGTAGTAGAA
CAATGTTTGATCAATAAGAAGAACAATGCCTTGAGATGCTCATGGCTCAAGAGGAAATCGAAATTCACTGATGTCGATGTACGTATCATAGACGATGAAG
TTACCATCAAGATCGTGCAGAAGAAGAAAATCAATTGTTTGTTGTTCGTATCCAAAGTCGTTGACCAACTTGAGCTTGATCTTCACCATGTAGCTGGTGC
ACAAATCGGAGAGCACCACAGTTTCTTGTTCAACGCCAAGATTAGTGAAGGATCTAGTGTTTATGCAAGTGCCATAGCAGATAGAGTAATGGAAGTGTTG
AAGAAACAATACATGGAAGCTCTTTCGGCGAATAATGGTTATCACTGTTACTCTAGTGAT

>IE_04B05_translated
MGGGGMFEEIGCFDPNAPAEMTAESSFSPSEPPPTITVIGSNSNSNCSLEDLSAFHLSPQDSSLPASASAYAHQLHINATPNCDHQFQSSMHQTLQDPSY
AQQSNHWDNGYQDFVNLGPNHTTPDLLSLLQLPRSSLPPFANPSIQDIIMTTSSSVAAYDPLFHLNFPLQPPNGSFMGVDQDQTETNQGVNLMYDEENNN
LDDGLNRKGRGSKKRKIFPTERERRVHFKDRFGDLKNLIPNPTKNDRASIVGEAIDYIKELLRTIDEFKLLVEKKRVKQRNREGDDVVDENFKAQSEVVE
QCLINKKNNALRCSWLKRKSKFTDVDVRIIDDEVTIKIVQKKKINCLLFVSKVVDQLELDLHHVAGAQIGEHHSFLFNAKISEGSSVYASAIADRVMEVL
KKQYMEALSANNGYHCYSSD
Alignment ◆CDS（TAIRver.9）_without_stop_codonとのalignment◆

100%一致しました。

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