◆◆◆IE_04C06（IE_09A10） detail◆◆◆

Code nameIE_04C06（IE_09A10）
LocusAT3G25790
TemplateIT_04C06 (HR1835)
Donor VectorpDONR207
Forward primerIPF_04C06 (ggggacaagtttgtacaaaaaagcaggcttcATGATCAAGAACTTAAGTAATATGAAGA)
Reverse primerIPR_04C06 (ggggaccactttgtacaagaaagctgggttTGATATTATTTTTGCATCTTTCGTCTTTGT)
Alignment with template seq.1stアミノ酸配列99.70%一致2ndアミノ酸配列100%一致
Alignment with TAIR7CDSアミノ酸配列99.40%一致アミノ酸配列99.40%一致
comment1st:(Sequence解析まで行ったが、フレームはずれないが塩基置換がある)→2nd(09A10):Templateの配列とは一致, nt.110G→T
sequence 
>IE_04C06_1068bp
ATGATCAAGAACTTAAGTAATATGAAGAATGACAATCAGAAACGTGAAAAATGTTGCGAGTACATCGAAGCTCTCGAAGAAGAACGTCGCAAGATCAATG
TTTTCCAACGCGAGCTTCCTCTTTGCGTAGAGCTCGTGACTCAAGCGATTGAGGCGTATAAGAGGGAGATATCAGGGACGTCGACGGATAACTTATACGG
ACAGTCGGAGTGCTCAGAGCAGACCACCGGAGAATGTGGGCGCATCTTGGATCTGTTCATACCAATCAAACACTCTTCGACCTCCATAGAAGAAGAGGTT
GATGACAAAGATGATGATGATGAAGAACACCAGTCTCATGAAACCGACATAGATTTTGATGACAAGAACATGAAATCTGAATGGCTCAAGTCTGTTCAGC
TCTGGAACCAATCCGACGCAGTTGTTTCTAATAATAGACAGGATCGCTCACAAGAAAAGACAGAGACGCTGGTAGAGTTGATAAAAATAAACGATGAAGC
AGCGAAGAAAAACAACAACATAAAATCACCGGTGACTACTAGCGATGGTGGCAGTGGCGGTGGCGGTGGCAGAAGAGGGCAGAGAAAGAATAGGCGGTGT
TGGTCGCAAGAGTTACACAGACGCTTCTTGAACGCTCTTAAACAACTTGGTGGCCCTCATGCTACGCCGAAACAGATAAGAGATATAATGAAGGTTGATG
GGTTAACAAATGATGAAGTCAAAAGTCATTTACAGAAATATAGGCTGCATGCAAGACGGCCAAGCCAAACAACACCGAACAACAGAAACTCTCAAACGCA
ACATTTCGTGGTGGTTGGTGGAATATGGGTCCCACAGACTAACCATTCAACGGCCAACGCAGTCAACGCCGTCGCATCGGGTGAGACCACCGGGATTTAT
GGGCCTATGGTCAGTTCGTTGCCGTCCGAGTGGCCGAGGCATTCCAATTTTGGTAGGAAGATTTCGGAAGATAGATCAAGATGCTCTAACAACGGATTTT
TTCGGTGTAGCTCACCAGCTATGTCTTGTTCTACTCGTACAAAGACGAAAGATGCAAAAATAATATCA

>IE_04C06_translated
MIKNLSNMKNDNQKREKCCEYIEALEEERRKINVFQRELPLCVELVTQAIEAYKREISGTSTDNLYGQSECSEQTTGECGRILDLFIPIKHSSTSIEEEV
DDKDDDDEEHQSHETDIDFDDKNMKSEWLKSVQLWNQSDAVVSNNRQDRSQEKTETLVELIKINDEAAKKNNNIKSPVTTSDGGSGGGGGRRGQRKNRRC
WSQELHRRFLNALKQLGGPHATPKQIRDIMKVDGLTNDEVKSHLQKYRLHARRPSQTTPNNRNSQTQHFVVVGGIWVPQTNHSTANAVNAVASGETTGIY
GPMVSSLPSEWPRHSNFGRKISEDRSRCSNNGFFRCSSPAMSCSTRTKTKDAKIIS
Alignment ◆CDS（TAIRver.9）_without_stop_codonとのalignment◆

1068/1071 (99.7%)（アミノ酸配列で356/357 (99.7%)）一致しました。


               10        20        30        40        50        60        70        80        90       100
AT3G25 ATGATCAAGAACTTAAGTAATATGAAGAATGACAATCAGAAACGTGAAAAATGTTGCGAGTACATCGAAGCTCTCGAAGAAGAACGTCGCAAGATCAATG
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_04C ATGATCAAGAACTTAAGTAATATGAAGAATGACAATCAGAAACGTGAAAAATGTTGCGAGTACATCGAAGCTCTCGAAGAAGAACGTCGCAAGATCAATG
               10        20        30        40        50        60        70        80        90       100

              110       120       130       140       150       160       170       180       190       200
AT3G25 TTTTCCAACGCGAGCTTCCTCTTTGCGTAGAGCTCGTGACTCAAGCGATTGAGGCGTATAAGAGGGAGATATCAGGGACGTCGACGGATAACTTATACGG
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_04C TTTTCCAACGCGAGCTTCCTCTTTGCGTAGAGCTCGTGACTCAAGCGATTGAGGCGTATAAGAGGGAGATATCAGGGACGTCGACGGATAACTTATACGG
              110       120       130       140       150       160       170       180       190       200

              210       220       230       240       250       260       270       280       290       300
AT3G25 ACAGTCGGAGTGCTCAGAGCAGACCACCGGAGAATGTGGGCGCATCTTGGATCTGTTCATACCAATCAAACACTCTTCGACCTCCATAGAAGAAGAGGTT
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_04C ACAGTCGGAGTGCTCAGAGCAGACCACCGGAGAATGTGGGCGCATCTTGGATCTGTTCATACCAATCAAACACTCTTCGACCTCCATAGAAGAAGAGGTT
              210       220       230       240       250       260       270       280       290       300

              310       320       330       340       350       360       370       380       390       400
AT3G25 GATGACAAAGATGATGATGATGAAGAACACCAGTCTCATGAAACCGACATAGATTTTGATGACAAGAACATGAAATCTGAATGGCTCAAGTCTGTTCAGC
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_04C GATGACAAAGATGATGATGATGAAGAACACCAGTCTCATGAAACCGACATAGATTTTGATGACAAGAACATGAAATCTGAATGGCTCAAGTCTGTTCAGC
              310       320       330       340       350       360       370       380       390       400

              410       420       430       440       450       460       470       480       490       500
AT3G25 TCTGGAACCAATCCGACGCAGTTGTTTCTAATAATAGACAGGATCGCTCACAAGAAAAGACAGAGACGCTGGTAGAGTTGATAAAAATAAACGATGAAGC
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_04C TCTGGAACCAATCCGACGCAGTTGTTTCTAATAATAGACAGGATCGCTCACAAGAAAAGACAGAGACGCTGGTAGAGTTGATAAAAATAAACGATGAAGC
              410       420       430       440       450       460       470       480       490       500

              510       520       530       540       550       560       570       580       590       600
AT3G25 AGCGAAGAAAAACAACAACATAAAATCACCGGTGACTACTAGCGATGGTGGCAGTGGCGGTGGCGGTGGCAGAAGAGGGCAGAGAAAGAATAGGCGGTGT
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_04C AGCGAAGAAAAACAACAACATAAAATCACCGGTGACTACTAGCGATGGTGGCAGTGGCGGTGGCGGTGGCAGAAGAGGGCAGAGAAAGAATAGGCGGTGT
              510       520       530       540       550       560       570       580       590       600

              610       620       630       640       650       660       670       680       690       700
AT3G25 TGGTCGCAAGAGTTACACAGACGCTTCTTGAACGCTCTTAAACAACTTGGTGGCCCTCATGTAGCTACGCCGAAACAGATAAGAGATATAATGAAGGTTG
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::   :::::::::::::::::::::::::::::::::::::
IE_04C TGGTCGCAAGAGTTACACAGACGCTTCTTGAACGCTCTTAAACAACTTGGTGGCCCTCAT---GCTACGCCGAAACAGATAAGAGATATAATGAAGGTTG
              610       620       630       640       650       660          670       680       690       

              710       720       730       740       750       760       770       780       790       800
AT3G25 ATGGGTTAACAAATGATGAAGTCAAAAGTCATTTACAGAAATATAGGCTGCATGCAAGACGGCCAAGCCAAACAACACCGAACAACAGAAACTCTCAAAC
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_04C ATGGGTTAACAAATGATGAAGTCAAAAGTCATTTACAGAAATATAGGCTGCATGCAAGACGGCCAAGCCAAACAACACCGAACAACAGAAACTCTCAAAC
       700       710       720       730       740       750       760       770       780       790       

              810       820       830       840       850       860       870       880       890       900
AT3G25 GCAACATTTCGTGGTGGTTGGTGGAATATGGGTCCCACAGACTAACCATTCAACGGCCAACGCAGTCAACGCCGTCGCATCGGGTGAGACCACCGGGATT
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_04C GCAACATTTCGTGGTGGTTGGTGGAATATGGGTCCCACAGACTAACCATTCAACGGCCAACGCAGTCAACGCCGTCGCATCGGGTGAGACCACCGGGATT
       800       810       820       830       840       850       860       870       880       890       

              910       920       930       940       950       960       970       980       990      1000
AT3G25 TATGGGCCTATGGTCAGTTCGTTGCCGTCCGAGTGGCCGAGGCATTCCAATTTTGGTAGGAAGATTTCGGAAGATAGATCAAGATGCTCTAACAACGGAT
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_04C TATGGGCCTATGGTCAGTTCGTTGCCGTCCGAGTGGCCGAGGCATTCCAATTTTGGTAGGAAGATTTCGGAAGATAGATCAAGATGCTCTAACAACGGAT
       900       910       920       930       940       950       960       970       980       990       

             1010      1020      1030      1040      1050      1060      1070 
AT3G25 TTTTTCGGTGTAGCTCACCAGCTATGTCTTGTTCTACTCGTACAAAGACGAAAGATGCAAAAATAATATCA
       :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_04C TTTTTCGGTGTAGCTCACCAGCTATGTCTTGTTCTACTCGTACAAAGACGAAAGATGCAAAAATAATATCA
      1000      1010      1020      1030      1040      1050      1060        




               10        20        30        40        50        60        70        80        90       100
AT3G25 MIKNLSNMKNDNQKREKCCEYIEALEEERRKINVFQRELPLCVELVTQAIEAYKREISGTSTDNLYGQSECSEQTTGECGRILDLFIPIKHSSTSIEEEV
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_04C MIKNLSNMKNDNQKREKCCEYIEALEEERRKINVFQRELPLCVELVTQAIEAYKREISGTSTDNLYGQSECSEQTTGECGRILDLFIPIKHSSTSIEEEV
               10        20        30        40        50        60        70        80        90       100

              110       120       130       140       150       160       170       180       190       200
AT3G25 DDKDDDDEEHQSHETDIDFDDKNMKSEWLKSVQLWNQSDAVVSNNRQDRSQEKTETLVELIKINDEAAKKNNNIKSPVTTSDGGSGGGGGRRGQRKNRRC
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_04C DDKDDDDEEHQSHETDIDFDDKNMKSEWLKSVQLWNQSDAVVSNNRQDRSQEKTETLVELIKINDEAAKKNNNIKSPVTTSDGGSGGGGGRRGQRKNRRC
              110       120       130       140       150       160       170       180       190       200

              210       220       230       240       250       260       270       280       290       300
AT3G25 WSQELHRRFLNALKQLGGPHVATPKQIRDIMKVDGLTNDEVKSHLQKYRLHARRPSQTTPNNRNSQTQHFVVVGGIWVPQTNHSTANAVNAVASGETTGI
       :::::::::::::::::::: :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_04C WSQELHRRFLNALKQLGGPH-ATPKQIRDIMKVDGLTNDEVKSHLQKYRLHARRPSQTTPNNRNSQTQHFVVVGGIWVPQTNHSTANAVNAVASGETTGI
              210       220        230       240       250       260       270       280       290         

              310       320       330       340       350       
AT3G25 YGPMVSSLPSEWPRHSNFGRKISEDRSRCSNNGFFRCSSPAMSCSTRTKTKDAKIIS
       :::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_04C YGPMVSSLPSEWPRHSNFGRKISEDRSRCSNNGFFRCSSPAMSCSTRTKTKDAKIIS
     300       310       320       330       340       350

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