◆◆◆IE_04C07 detail◆◆◆

Code nameIE_04C07
LocusAT3G49950
TemplateIT_04C07 (HR1885)
Donor VectorpDONR207
Forward primerIPF_04C07 (ggggacaagtttgtacaaaaaagcaggcttcATGACCAAAACCCGGATTCTCAACCCGA)
Reverse primerIPR_04C07 (ggggaccactttgtacaagaaagctgggttAATGGGAACCCAAACGGTAGCAAACACAAC)
Alignment with template seq.1stアミノ酸配列100%一致2nd-
Alignment with TAIR7CDSアミノ酸配列100%一致-
comment
sequence 
>IE_04C07_1230bp
ATGACCAAAACCCGGATTCTCAACCCGACCCGATTCCCTTCTCCCAAACCCTTACGTGGCTGCGGCGACGCTAACTTCATGGAACAGCTCCTCCTTCACT
GCGCCACCGCCATAGACTCCAACGACGCTGCATTAACTCACCAAATCCTCTGGGTCCTCAACAACATCGCTCCACCAGACGGTGACTCCACCCAACGTCT
TACATCCGCATTCCTCCGCGCGTTGCTCTCACGCGCCGTCTCCAAAACCCCTACACTATCATCCACCATTAGCTTCCTACCTCAGGCCGATGAACTCCAT
CGTTTCTCAGTCGTGGAGTTAGCCGCTTTCGTAGACCTCACTCCGTGGCACCGGTTCGGATTCATTGCAGCCAACGCCGCGATCTTAACCGCCGTTGAAG
GTTACTCAACGGTTCACATCGTTGACTTAAGTTTGACTCATTGTATGCAAATCCCTACTCTTATAGACGCCATGGCAAGCCGACTCAACAAACCTCCTCC
GCTTCTTAAACTCACCGTCGTCTCTTCCTCCGACCACTTCCCACCATTCATCAACATCTCCTACGAAGAACTCGGATCTAAACTAGTCAACTTCGCCACC
ACACGAAACATTACAATGGAGTTCACAATCGTACCTTCTACTTACTCCGATGGCTTCTCCTCCCTCTTACAACAGTTACGGATTTATCCTTCTTCATTCA
ACGAAGCTCTCGTCGTTAACTGTCACATGATGCTCCGTTACATCCCTGAAGAACCCCTAACTTCATCATCATCATCGCTCCGAACAGTTTTCTTGAAACA
ACTCCGATCTTTGAATCCAAGAATCGTAACCCTAATCGAAGAAGACGTAGATCTCACATCGGAGAACTTGGTTAACCGGTTAAAATCGGCGTTTAACTAC
TTCTGGATTCCGTTCGATACGACGGACACGTTTATGAGTGAGCAGAGACGGTGGTACGAGGCGGAGATAAGTTGGAAGATAGAAAACGTGGTGGCGAAGG
AAGGTGCGGAGAGAGTGGAGAGGACGGAGACGAAGAGACGGTGGATAGAGAGAATGAGAGAGGCTGAGTTCGGTGGAGTTAGGGTTAAAGAAGATGCGGT
GGCGGATGTGAAGGCGATGCTGGAGGAGCACGCTGTCGGGTGGGGAATGAAGAAGGAAGACGACGACGAGAGTCTCGTGCTGACGTGGAAAGGTCATAGT
GTTGTGTTTGCTACCGTTTGGGTTCCCATT

>IE_04C07_translated
MTKTRILNPTRFPSPKPLRGCGDANFMEQLLLHCATAIDSNDAALTHQILWVLNNIAPPDGDSTQRLTSAFLRALLSRAVSKTPTLSSTISFLPQADELH
RFSVVELAAFVDLTPWHRFGFIAANAAILTAVEGYSTVHIVDLSLTHCMQIPTLIDAMASRLNKPPPLLKLTVVSSSDHFPPFINISYEELGSKLVNFAT
TRNITMEFTIVPSTYSDGFSSLLQQLRIYPSSFNEALVVNCHMMLRYIPEEPLTSSSSSLRTVFLKQLRSLNPRIVTLIEEDVDLTSENLVNRLKSAFNY
FWIPFDTTDTFMSEQRRWYEAEISWKIENVVAKEGAERVERTETKRRWIERMREAEFGGVRVKEDAVADVKAMLEEHAVGWGMKKEDDDESLVLTWKGHS
VVFATVWVPI
Alignment ◆CDS（TAIRver.9）_without_stop_codonとのalignment◆

100%一致しました。

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