◆◆◆IE_04C08 detail◆◆◆

Code nameIE_04C08
LocusAT5G62940
TemplateIT_04C08 (HR1919)
Donor VectorpDONR207
Forward primerIPF_04C08 (ggggacaagtttgtacaaaaaagcaggcttcATGGGTCTCACTTCTCTTCAAGTTTGCA)
Reverse primerIPR_04C08 (ggggaccactttgtacaagaaagctgggttAACCAAGGAGTTTGTTTTAGTGGACGTTCC)
Alignment with template seq.1stアミノ酸配列100%一致2nd-
Alignment with TAIR7CDSアミノ酸配列100%一致-
comment
sequence 
>IE_04C08_1116bp
ATGGGTCTCACTTCTCTTCAAGTTTGCATGGATTCTGATTGGCTCCAGGAATCCGAGTCATCAGGAGGAAGCATGTTAGACTCTTCAACGAATTCTCCGT
CAGCAGCCGACATACTAGCAGCTTGCAGCACTAGACCACAAGCCTCGGCCGTGGCTGTAGCCGCTGCAGCTCTGATGGACGGTGGAAGGAGGCTGCGTCC
ACCTCACGACCATCCTCAAAAGTGTCCTCGTTGCGAGTCAACACATACTAAGTTCTGTTACTACAATAACTACAGCCTCTCTCAGCCTCGTTACTTCTGC
AAGACTTGTCGCCGTTACTGGACAAAAGGCGGAACTCTAAGGAATATTCCGGTTGGTGGTGGATGCCGTAAAAACAAGAAACCATCTTCCTCTAATTCCT
CCTCCTCCACTTCTTCCGGCAAAAAACCATCCAACATCGTTACCGCCAATACCTCTGATCTTATGGCTTTAGCACATTCTCATCAAAATTACCAACATTC
TCCTCTAGGGTTTTCACATTTTGGTGGGATGATGGGGTCTTACTCAACTCCGGAGCATGGTAACGTTGGTTTCTTGGAGAGCAAGTATGGCGGTTTGCTT
TCGCAGAGCCCTAGACCTATTGATTTCTTGGACAGTAAGTTTGATCTCATGGGAGTGAACAATGACAACCTGGTCATGGTTAATCATGGAAGTAACGGAG
ATCATCATCATCATCATAATCATCACATGGGTCTGAATCACGGTGTAGGTCTTAACAACAACAACAACAATGGTGGATTTAATGGGATTTCTACGGGAGG
CAATGGAAATGGTGGTGGTCTCATGGATATATCGACATGCCAAAGACTTATGCTATCTAATTATGATCATCACCATTACAATCATCAAGAAGATCATCAA
AGGGTAGCAACAATAATGGATGTGAAGCCAAATCCGAAGTTGTTATCGCTTGATTGGCAGCAAGATCAATGCTACTCCAATGGTGGTGGTAGCGGAGGCG
CAGGAAAATCCGACGGTGGTGGATACGGCAATGGTGGTTATATCAACGGTTTAGGTTCGTCGTGGAATGGTTTGATGAATGGCTATGGAACGTCCACTAA
AACAAACTCCTTGGTT

>IE_04C08_translated
MGLTSLQVCMDSDWLQESESSGGSMLDSSTNSPSAADILAACSTRPQASAVAVAAAALMDGGRRLRPPHDHPQKCPRCESTHTKFCYYNNYSLSQPRYFC
KTCRRYWTKGGTLRNIPVGGGCRKNKKPSSSNSSSSTSSGKKPSNIVTANTSDLMALAHSHQNYQHSPLGFSHFGGMMGSYSTPEHGNVGFLESKYGGLL
SQSPRPIDFLDSKFDLMGVNNDNLVMVNHGSNGDHHHHHNHHMGLNHGVGLNNNNNNGGFNGISTGGNGNGGGLMDISTCQRLMLSNYDHHHYNHQEDHQ
RVATIMDVKPNPKLLSLDWQQDQCYSNGGGSGGAGKSDGGGYGNGGYINGLGSSWNGLMNGYGTSTKTNSLV
Alignment ◆CDS（TAIRver.9）_without_stop_codonとのalignment◆

100%一致しました。

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