◆◆◆IE_04C09（IE_09A11） detail◆◆◆

Code nameIE_04C09（IE_09A11）
LocusAT1G34670
TemplateIT_04C09 (HR1986)
Donor VectorpDONR207
Forward primerIPF_04C09 (ggggacaagtttgtacaaaaaagcaggcttcATGGGGAGGTCGCCTTGTTGCGACGAGA)
Reverse primerIPR_04C09 (ggggaccactttgtacaagaaagctgggttAGATATAACGTTCATGAGGCTTTCGTCGAA)
Alignment with template seq.1st配列解析が行えなかったサンプルです。2ndアミノ酸配列100%一致
Alignment with TAIR7CDS-アミノ酸配列100%一致
comment1st:(PCRで目的サイズの増幅が確認できたが、コロニーが得られなかった)→2nd(09A11):100%
sequence 
>IE_04C09_1095bp
ATGGGGAGGTCGCCTTGTTGCGACGAGAATGGTCTAAAGAAAGGGCCATGGACTCCTGAAGAAGATCAAAAGCTTATTGATTACATTCATAAACATGGTC
ATGGAAGCTGGAGAGCTCTCCCCAAGCTCGCAGATTTGAATAGGTGTGGAAAGAGTTGTAGACTGAGGTGGACAAATTATTTGAGACCAGACATCAAAAG
AGGAAAGTTTTCAGCCGAAGAAGAGCAAACAATCCTCCATCTCCACTCCATTCTCGGGAATAAATGGTCAGCTATTGCGACGCATTTGCAAGGTCGTACA
GATAATGAGATAAAAAACTTTTGGAACACACATCTAAAGAAGAAATTGATCCAGATGGGGATCGATCCTGTGACTCATCAGCCAAGAACCGACCTCTTCG
CAAGTTTACCTCAGCTCATAGCCTTGGCAAATCTAAAAGACCTAATCGAACAAACATCACAATTCTCATCCATGCAAGGCGAAGCAGCCCAACTAGCCAA
TCTACAATATCTCCAACGTATGTTTAATTCTTCGGCTTCATTAACCAACAACAACGGCAACAATTTTAGCCCGAGTAGTATTCTCGATATCGATCAGCAC
CATGCCATGAACCTCTTAAACTCCATGGTCTCTTGGAACAAAGATCAAAACCCCGCTTTTGATCCGGTTCTTGAACTCGAGGCTAACGATCAAAATCAAG
ATTTATTTCCACTAGGGTTTATCATTGATCAACCCACCCAACCACTCCAACAACAAAAATACCATTTAAACAATAGTCCTAGTGAGCTACCATCGCAAGG
TGACCCACTTCTTGATCATGTCCCTTTCAGTCTCCAAACACCTTTGAATAGTGAGGATCACTTTATAGATAATTTGGTCAAACATCCAACTGATCATGAG
CATGAACATGATGACAATCCATCTTCATGGGTTCTCCCTTCTCTTATAGACAATAACCCTAAAACTGTCACGTCTTCTCTTCCACACAACAATCCGGCGG
ATGCAAGTAGCAGCTCAAGCTATGGAGGTTGTGAAGCTGCGTCGTTTTATTGGCCTGACATTTGCTTCGACGAAAGCCTCATGAACGTTATATCT

>IE_04C09_translated
MGRSPCCDENGLKKGPWTPEEDQKLIDYIHKHGHGSWRALPKLADLNRCGKSCRLRWTNYLRPDIKRGKFSAEEEQTILHLHSILGNKWSAIATHLQGRT
DNEIKNFWNTHLKKKLIQMGIDPVTHQPRTDLFASLPQLIALANLKDLIEQTSQFSSMQGEAAQLANLQYLQRMFNSSASLTNNNGNNFSPSSILDIDQH
HAMNLLNSMVSWNKDQNPAFDPVLELEANDQNQDLFPLGFIIDQPTQPLQQQKYHLNNSPSELPSQGDPLLDHVPFSLQTPLNSEDHFIDNLVKHPTDHE
HEHDDNPSSWVLPSLIDNNPKTVTSSLPHNNPADASSSSSYGGCEAASFYWPDICFDESLMNVIS
Alignment ◆CDS（TAIRver.9）_without_stop_codonとのalignment◆

100%一致しました。

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