◆◆◆IE_04C11 detail◆◆◆

Code nameIE_04C11
LocusAT5G11050
TemplateIT_04C11 (HR2136)
Donor VectorpDONR207
Forward primerIPF_04C11 (ggggacaagtttgtacaaaaaagcaggcttcATGGAAGAACAGAAAATTCAAGAGAAGA)
Reverse primerIPR_04C11 (ggggaccactttgtacaagaaagctgggttGAACAATGGGAACCAAATGTTGCTACCTTG)
Alignment with template seq.1stアミノ酸配列100%一致2nd-
Alignment with TAIR7CDSアミノ酸配列100%一致-
comment
sequence 
>IE_04C11_1269bp
ATGGAAGAACAGAAAATTCAAGAGAAGAGCCTCGCTCACGGCGCTGCACCACCGTTGACGGCGGTTGAGAGATTCTTGAACGGCCAAAAGAACGAAGCCT
TGTGCTTCAAGAAACAAGAACGTTCTATAGACCGACCGATCGTGAAGACGACAAGGGCGATTGAGATTAGAAATGAAAACAAAGAGAACATGATGTTTGG
TCCGAGGAAGGAGAAAAACTTGGCCGTTATTGGAGAAATCGTCGTTAAAGGTGCAGCTAAAGATTATACTTGCAAGGATATAACAAAGAAGCAACCATAC
AAGAATATCATCAAAGGGCAATGGACAGCCGATGAAGACAGGAAGTTGATAAAGTTGGTGATGCAACATGGAGAGAGGAAATGGGCAGTAATCTCGGAGA
AGCTTGAGGGAAGAGCAGGCAAGCAATGTCGTGAGAGATGGCATAATCATCTCCGTCCTGATATCAAGAAAGATAGTTGGAGTGAAGAAGAAGAGAGACT
TCTTGTGGAAGCACACACCAGAATCGGGAATAAGTGGGCAGAGATCGCTAAACTCATACAAGGGCGAACCGAAAACTCGATCAAGAACCATTGGAATGCG
ACAAAACGACGCCAAAACTCAAAACGCAAACATAAGCGTTCAAAAAACGCGGATAGTAACAGCGATATTGATGATCTCTCTCCATCCGCTAAAAGGCCGC
GCATTCTCGAAGACTATATTAAAAACATCGAGAATAATGACAAGAACAATGGCGAGAACATCATGACTACTAGTGGTAACAATGTTCTTTCGACTTCGAA
TTATGATCAGTTCAATTCTGAAGACTCTACATCGTCACTCTTGGATGATCCTTATGACGAAGAGCTTGTTTTCCTGAAAAATATCTTTGAGAACCATTCG
CTCGAGAACATCAATTTGAGCCAAGGTACGGAGATAACCCAGTCTTCTTCATCTGGGTTTATGATCGAGAACCCTAAACCTAAACCTAACTTGTACAACA
ATACTTTTGGAACACATCTTGGAGCAATGGTCACAGAACCTGCCAATTCTTCCCACCTCGCATCTGATATCTACTTATCTGATTTGTTGAACGGTACGGC
GTCGTCTTCTTCTTCACTTACTTTTCTGTCTTCCAACAACAATGAGCATGCCGGTGAAAACGAGTTGCTTTTGCCCCAAGCTAACTCCACTAGCGAAAGA
AGAGAAATGGATCTGATAGAAATGTTGTCTGGTTCTACTCAAGGTAGCAACATTTGGTTCCCATTGTTC

>IE_04C11_translated
MEEQKIQEKSLAHGAAPPLTAVERFLNGQKNEALCFKKQERSIDRPIVKTTRAIEIRNENKENMMFGPRKEKNLAVIGEIVVKGAAKDYTCKDITKKQPY
KNIIKGQWTADEDRKLIKLVMQHGERKWAVISEKLEGRAGKQCRERWHNHLRPDIKKDSWSEEEERLLVEAHTRIGNKWAEIAKLIQGRTENSIKNHWNA
TKRRQNSKRKHKRSKNADSNSDIDDLSPSAKRPRILEDYIKNIENNDKNNGENIMTTSGNNVLSTSNYDQFNSEDSTSSLLDDPYDEELVFLKNIFENHS
LENINLSQGTEITQSSSSGFMIENPKPKPNLYNNTFGTHLGAMVTEPANSSHLASDIYLSDLLNGTASSSSSLTFLSSNNNEHAGENELLLPQANSTSER
REMDLIEMLSGSTQGSNIWFPLF
Alignment ◆CDS（TAIRver.9）_without_stop_codonとのalignment◆

100%一致しました。

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