◆◆◆IE_04C12 detail◆◆◆

Code name	IE_04C12
Locus	AT5G07700
Template	IT_04C12 (HR2148)
Donor Vector	pDONR207
Forward primer	IPF_04C12 (ggggacaagtttgtacaaaaaagcaggcttcATGTCAAAGAGACCATATTGTATCGGAG)
Reverse primer	IPR_04C12 (ggggaccactttgtacaagaaagctgggttTAAGAAGTTCTTCTCGTCGGAATCTTGATC)
Alignment with template seq.	1st	アミノ酸配列100%一致	2nd	-
Alignment with TAIR7CDS	1st	アミノ酸配列99.70%一致	2nd	-
comment	1st:Templateの配列とは一致
sequence	>IE_04C12_1014bp ATGTCAAAGAGACCATATTGTATCGGAGAAGGACTGAAGAAAGGAGCATGGACTACAGAAGAGGATAAAAAACTCATCTCTTATATCCACGACCACGGTG AAGGAGGCTGGCGTGACATTCCAGAAAAAGCTGGGCTGAAACGGTGTGGAAAGAGTTGTAGATTACGGTGGACTAACTATTCGAAACCAGATATCAAGAG AGGAGAGTTTAGCTATGAGGAAGAGCAGATTATCATCATGCTTCATGCATCTCGTGGCAATAAGTGGTCTGTCATAGCTAGACATTTGCCAAAAAGAACG GATAACGAGGTCAAAAACTATTGGAACACACATCTCAAGAAACGTTTAATCGATGATGGCATTGATCCCGTGACACACAAGCCACTAGCTTCTTCTAACC CTAATCCAGTTGAGCCCATGAAGTTCGATTTCCAAAAGAAATCCAATCAGGATGAGCACTCTTCACAGTCTAGTTCTACAACTCCAGCATCTCTTCCCCT TTCCTCGAATTTGAACAGTGTTAAATCCAAAATTAGCAGTGGTGAGACGCAGATAGAAAGTGGTCACGTGAGCTGCAAGAAACGTTTTGGACGATCGAGC TCTACATCAAGGTTGTTAAACAAAGTTGCAGCTAGAGCTTCTTCCATCGGCAACATCTTATCAACATCCATAGAAGGAACCTTGAGATCTCCTGCATCAT CTTCAGGACTCCCAGACTCGTTCTCTCAATCATATGAGTACATGATCGATAACAAAGAAGATCTCGGTACGAGCATTGATCTCAACATCCCCGAGTATGA TTTCCCACAGTTTCTTGAGCAACTCATTAACGATGACGACGAAAATGAGAACATTGTTGGGCCCGAACAAGATCTCCTTATGTCCGATTTCCCATCAACA TTCGTTGATGAAGACGATATACTTGGAGACATAACCAGTTGGTCAACTTATCTTCTTGACCATCCCAATTTTATGTATGAATCGGATCAAGATTCCGACG AGAAGAACTTCTTA >IE_04C12_translated MSKRPYCIGEGLKKGAWTTEEDKKLISYIHDHGEGGWRDIPEKAGLKRCGKSCRLRWTNYSKPDIKRGEFSYEEEQIIIMLHASRGNKWSVIARHLPKRT DNEVKNYWNTHLKKRLIDDGIDPVTHKPLASSNPNPVEPMKFDFQKKSNQDEHSSQSSSTTPASLPLSSNLNSVKSKISSGETQIESGHVSCKKRFGRSS STSRLLNKVAARASSIGNILSTSIEGTLRSPASSSGLPDSFSQSYEYMIDNKEDLGTSIDLNIPEYDFPQFLEQLINDDDENENIVGPEQDLLMSDFPST FVDEDDILGDITSWSTYLLDHPNFMYESDQDSDEKNFL
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 1013/1014 (99.9%)（アミノ酸配列で337/338 (99.7%)）一致しました。 10 20 30 40 50 60 70 80 90 100 AT5G07 ATGTCAAAGAGACCATATTGTATCGGAGAAGGACTGAAGAAAGGAGCATGGACTACAGAAGAGGATAAAAAACTCATCTCTTATATCCACGACCACGGTG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04C ATGTCAAAGAGACCATATTGTATCGGAGAAGGACTGAAGAAAGGAGCATGGACTACAGAAGAGGATAAAAAACTCATCTCTTATATCCACGACCACGGTG 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT5G07 AAGGAGGCTGGCGTGACATTCCAGAAAAAGCTGGGCTGAAACGGTGTGGAAAGAGTTGTAGATTACGGTGGACTAACTATTTGAAACCAGATATCAAGAG ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: :::::::::::::::::: IE_04C AAGGAGGCTGGCGTGACATTCCAGAAAAAGCTGGGCTGAAACGGTGTGGAAAGAGTTGTAGATTACGGTGGACTAACTATTCGAAACCAGATATCAAGAG 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT5G07 AGGAGAGTTTAGCTATGAGGAAGAGCAGATTATCATCATGCTTCATGCATCTCGTGGCAATAAGTGGTCTGTCATAGCTAGACATTTGCCAAAAAGAACG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04C AGGAGAGTTTAGCTATGAGGAAGAGCAGATTATCATCATGCTTCATGCATCTCGTGGCAATAAGTGGTCTGTCATAGCTAGACATTTGCCAAAAAGAACG 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 AT5G07 GATAACGAGGTCAAAAACTATTGGAACACACATCTCAAGAAACGTTTAATCGATGATGGCATTGATCCCGTGACACACAAGCCACTAGCTTCTTCTAACC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04C GATAACGAGGTCAAAAACTATTGGAACACACATCTCAAGAAACGTTTAATCGATGATGGCATTGATCCCGTGACACACAAGCCACTAGCTTCTTCTAACC 310 320 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 480 490 500 AT5G07 CTAATCCAGTTGAGCCCATGAAGTTCGATTTCCAAAAGAAATCCAATCAGGATGAGCACTCTTCACAGTCTAGTTCTACAACTCCAGCATCTCTTCCCCT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04C CTAATCCAGTTGAGCCCATGAAGTTCGATTTCCAAAAGAAATCCAATCAGGATGAGCACTCTTCACAGTCTAGTTCTACAACTCCAGCATCTCTTCCCCT 410 420 430 440 450 460 470 480 490 500 510 520 530 540 550 560 570 580 590 600 AT5G07 TTCCTCGAATTTGAACAGTGTTAAATCCAAAATTAGCAGTGGTGAGACGCAGATAGAAAGTGGTCACGTGAGCTGCAAGAAACGTTTTGGACGATCGAGC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04C TTCCTCGAATTTGAACAGTGTTAAATCCAAAATTAGCAGTGGTGAGACGCAGATAGAAAGTGGTCACGTGAGCTGCAAGAAACGTTTTGGACGATCGAGC 510 520 530 540 550 560 570 580 590 600 610 620 630 640 650 660 670 680 690 700 AT5G07 TCTACATCAAGGTTGTTAAACAAAGTTGCAGCTAGAGCTTCTTCCATCGGCAACATCTTATCAACATCCATAGAAGGAACCTTGAGATCTCCTGCATCAT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04C TCTACATCAAGGTTGTTAAACAAAGTTGCAGCTAGAGCTTCTTCCATCGGCAACATCTTATCAACATCCATAGAAGGAACCTTGAGATCTCCTGCATCAT 610 620 630 640 650 660 670 680 690 700 710 720 730 740 750 760 770 780 790 800 AT5G07 CTTCAGGACTCCCAGACTCGTTCTCTCAATCATATGAGTACATGATCGATAACAAAGAAGATCTCGGTACGAGCATTGATCTCAACATCCCCGAGTATGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04C CTTCAGGACTCCCAGACTCGTTCTCTCAATCATATGAGTACATGATCGATAACAAAGAAGATCTCGGTACGAGCATTGATCTCAACATCCCCGAGTATGA 710 720 730 740 750 760 770 780 790 800 810 820 830 840 850 860 870 880 890 900 AT5G07 TTTCCCACAGTTTCTTGAGCAACTCATTAACGATGACGACGAAAATGAGAACATTGTTGGGCCCGAACAAGATCTCCTTATGTCCGATTTCCCATCAACA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04C TTTCCCACAGTTTCTTGAGCAACTCATTAACGATGACGACGAAAATGAGAACATTGTTGGGCCCGAACAAGATCTCCTTATGTCCGATTTCCCATCAACA 810 820 830 840 850 860 870 880 890 900 910 920 930 940 950 960 970 980 990 1000 AT5G07 TTCGTTGATGAAGACGATATACTTGGAGACATAACCAGTTGGTCAACTTATCTTCTTGACCATCCCAATTTTATGTATGAATCGGATCAAGATTCCGACG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04C TTCGTTGATGAAGACGATATACTTGGAGACATAACCAGTTGGTCAACTTATCTTCTTGACCATCCCAATTTTATGTATGAATCGGATCAAGATTCCGACG 910 920 930 940 950 960 970 980 990 1000 1010 AT5G07 AGAAGAACTTCTTA :::::::::::::: IE_04C AGAAGAACTTCTTA 1010 10 20 30 40 50 60 70 80 90 100 AT5G07 MSKRPYCIGEGLKKGAWTTEEDKKLISYIHDHGEGGWRDIPEKAGLKRCGKSCRLRWTNYLKPDIKRGEFSYEEEQIIIMLHASRGNKWSVIARHLPKRT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: ::::::::::::::::::::::::::::::::::::::: IE_04C MSKRPYCIGEGLKKGAWTTEEDKKLISYIHDHGEGGWRDIPEKAGLKRCGKSCRLRWTNYSKPDIKRGEFSYEEEQIIIMLHASRGNKWSVIARHLPKRT 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT5G07 DNEVKNYWNTHLKKRLIDDGIDPVTHKPLASSNPNPVEPMKFDFQKKSNQDEHSSQSSSTTPASLPLSSNLNSVKSKISSGETQIESGHVSCKKRFGRSS :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04C DNEVKNYWNTHLKKRLIDDGIDPVTHKPLASSNPNPVEPMKFDFQKKSNQDEHSSQSSSTTPASLPLSSNLNSVKSKISSGETQIESGHVSCKKRFGRSS 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT5G07 STSRLLNKVAARASSIGNILSTSIEGTLRSPASSSGLPDSFSQSYEYMIDNKEDLGTSIDLNIPEYDFPQFLEQLINDDDENENIVGPEQDLLMSDFPST :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04C STSRLLNKVAARASSIGNILSTSIEGTLRSPASSSGLPDSFSQSYEYMIDNKEDLGTSIDLNIPEYDFPQFLEQLINDDDENENIVGPEQDLLMSDFPST 210 220 230 240 250 260 270 280 290 300 310 320 330 AT5G07 FVDEDDILGDITSWSTYLLDHPNFMYESDQDSDEKNFL :::::::::::::::::::::::::::::::::::::: IE_04C FVDEDDILGDITSWSTYLLDHPNFMYESDQDSDEKNFL 310 320 330

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