◆◆◆IE_04D01 detail◆◆◆

Code name	IE_04D01
Locus	AT5G40360
Template	IT_04D01 (HR2180)
Donor Vector	pDONR207
Forward primer	IPF_04D01 (ggggacaagtttgtacaaaaaagcaggcttcATGTATCACCAAAATCTGATTTCATCGA)
Reverse primer	IPR_04D01 (ggggaccactttgtacaagaaagctgggttATTCCAACCATTCATGAGCATCCATGTATC)
Alignment with template seq.	1st	アミノ酸配列100%一致	2nd	-
Alignment with TAIR7CDS	1st	アミノ酸配列99.70%一致	2nd	-
comment	1st:Templateの配列とは一致
sequence	>IE_04D01_1077bp ATGTATCACCAAAATCTGATTTCATCGACCCCAAACCAAAATTCTAATCCTCACGATTGGGATATTCAAAATCCTTTATTTTCCATACATCCTTCAGCTG AAATACCCTCAAAATATCCCTTTATGGGTATCACTTCTTGCCCAAACACCAATGTTTTTGAGGAATTTCAATATAAAATAACCAACGATCAAAACTTTCC AACGACCTATAACACCCCATTCCCTGTAATATCTGAAGGTATATCATACAATATGCATGATGTTCAAGAGAATACTATGTGTGGTTATACTGCTCACAAT CAAGGGCTTATCATTGGTTGTCACGAGCCAGTTCTTGTTCATGCCGTGGTTGAGTCTCAGCAATTTAATGTCCCACAGTCAGAGGATATCAATTTAGTAT CTCAGAGTGAGAGGGTCACAGAAGATAAGGTAATGTTCAAAACCGATCATAAGAAGAAAGACATTATTGGCAAAGGGCAATGGACTCCTACTGAAGACGA GTTGTTGGTGAGAATGGTGAAAAGTAAAGGAACGAAAAACTGGACATCAATCGCAAAAATGTTCCAAGGACGAGTAGGAAAACAATGTCGAGAGAGGTGG CGTAACCATCTTCGACCAAACATCAAGAAAAATGATTGGAGTGAAGAAGAAGATCAAATACTTATTGAAGTCCACAAGATAGTTGGCAACAAATGGACCG AAATTGCTAAAAGACTTCCTGGACGAAGTGAAAATATTGTTAAAAATCATTGGAATGCTACAAAACGTCGACTACACTCCGTAAGGACTAAGAGAAGTGA TGCTTTTTCCCCTCGCAACAATGCCCTTGAAAATTACATAAGGTCTATCACAATCAACAACAATGCCCTTATGAACAGAGAAGTTGATTCTATAACTGCA AACTCGGAAATTGATAGTACCAGATGTGAGAATATTGTTGACGAGGTGATGAATCTGAATCTGCACGCTACAACGTCCGTTTATGTTCCGGAGCAAGCAG TGTTGACATGGGGTTATGATTTTACAAAATGTTATGAACCCATGGATGATACATGGATGCTCATGAATGGTTGGAAT >IE_04D01_translated MYHQNLISSTPNQNSNPHDWDIQNPLFSIHPSAEIPSKYPFMGITSCPNTNVFEEFQYKITNDQNFPTTYNTPFPVISEGISYNMHDVQENTMCGYTAHN QGLIIGCHEPVLVHAVVESQQFNVPQSEDINLVSQSERVTEDKVMFKTDHKKKDIIGKGQWTPTEDELLVRMVKSKGTKNWTSIAKMFQGRVGKQCRERW RNHLRPNIKKNDWSEEEDQILIEVHKIVGNKWTEIAKRLPGRSENIVKNHWNATKRRLHSVRTKRSDAFSPRNNALENYIRSITINNNALMNREVDSITA NSEIDSTRCENIVDEVMNLNLHATTSVYVPEQAVLTWGYDFTKCYEPMDDTWMLMNGWN
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 1076/1077 (99.9%)（アミノ酸配列で358/359 (99.7%)）一致しました。 10 20 30 40 50 60 70 80 90 100 AT5G40 ATGTATCACCAAAATCTGATTTCATCGACCCCAAACCAAAATTCTAATCCTCACGATTGGGATATTCAAAATCCTTTATTTTCCATACATCCTTCAGCTG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04D ATGTATCACCAAAATCTGATTTCATCGACCCCAAACCAAAATTCTAATCCTCACGATTGGGATATTCAAAATCCTTTATTTTCCATACATCCTTCAGCTG 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT5G40 AAATACCCTCAAAATATCCCTTTATGGGTATCACTTCTTGCCCAAACACCAATGTTTTTGAGGAATTTCAATATAAAATAACCAACGATCAAAACTTTCC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04D AAATACCCTCAAAATATCCCTTTATGGGTATCACTTCTTGCCCAAACACCAATGTTTTTGAGGAATTTCAATATAAAATAACCAACGATCAAAACTTTCC 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT5G40 AACGACCTATAACACCCCATTCCCTGTAATATCTGAAGGTATATCATACAATATGCATGATGTTCAAGAGAATACTATGTGTGGTTATACTGCTCACAAT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04D AACGACCTATAACACCCCATTCCCTGTAATATCTGAAGGTATATCATACAATATGCATGATGTTCAAGAGAATACTATGTGTGGTTATACTGCTCACAAT 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 AT5G40 CAAGGGCTTATCATTGGTTGTCACGAGCCAGTTCTTGTTCATGCCGTGGTTGAGTCTCAGCAATTTAATGTCCCACAGTCAGAGGATATCAATTTAGTAT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04D CAAGGGCTTATCATTGGTTGTCACGAGCCAGTTCTTGTTCATGCCGTGGTTGAGTCTCAGCAATTTAATGTCCCACAGTCAGAGGATATCAATTTAGTAT 310 320 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 480 490 500 AT5G40 CTCAGAGTGAGAGGGTCACAGAAGATAAGGTAATGTTCAAAACCGATCATAAGAAGAAAGACATTATTGGCAAAGGGCAATGGACTCCTACTGAAGACGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04D CTCAGAGTGAGAGGGTCACAGAAGATAAGGTAATGTTCAAAACCGATCATAAGAAGAAAGACATTATTGGCAAAGGGCAATGGACTCCTACTGAAGACGA 410 420 430 440 450 460 470 480 490 500 510 520 530 540 550 560 570 580 590 600 AT5G40 GTTGTTGGTGAGAATGGTGAAAAGTAAAGGAACGAAAAACTGGACATCAATCGCAAAAATGTTCCAAGGACGAGTAGGAAAACAATGTCGAGAGAGGTGG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04D GTTGTTGGTGAGAATGGTGAAAAGTAAAGGAACGAAAAACTGGACATCAATCGCAAAAATGTTCCAAGGACGAGTAGGAAAACAATGTCGAGAGAGGTGG 510 520 530 540 550 560 570 580 590 600 610 620 630 640 650 660 670 680 690 700 AT5G40 CATAACCATCTTCGACCAAACATCAAGAAAAATGATTGGAGTGAAGAAGAAGATCAAATACTTATTGAAGTCCACAAGATAGTTGGCAACAAATGGACCG : :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04D CGTAACCATCTTCGACCAAACATCAAGAAAAATGATTGGAGTGAAGAAGAAGATCAAATACTTATTGAAGTCCACAAGATAGTTGGCAACAAATGGACCG 610 620 630 640 650 660 670 680 690 700 710 720 730 740 750 760 770 780 790 800 AT5G40 AAATTGCTAAAAGACTTCCTGGACGAAGTGAAAATATTGTTAAAAATCATTGGAATGCTACAAAACGTCGACTACACTCCGTAAGGACTAAGAGAAGTGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04D AAATTGCTAAAAGACTTCCTGGACGAAGTGAAAATATTGTTAAAAATCATTGGAATGCTACAAAACGTCGACTACACTCCGTAAGGACTAAGAGAAGTGA 710 720 730 740 750 760 770 780 790 800 810 820 830 840 850 860 870 880 890 900 AT5G40 TGCTTTTTCCCCTCGCAACAATGCCCTTGAAAATTACATAAGGTCTATCACAATCAACAACAATGCCCTTATGAACAGAGAAGTTGATTCTATAACTGCA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04D TGCTTTTTCCCCTCGCAACAATGCCCTTGAAAATTACATAAGGTCTATCACAATCAACAACAATGCCCTTATGAACAGAGAAGTTGATTCTATAACTGCA 810 820 830 840 850 860 870 880 890 900 910 920 930 940 950 960 970 980 990 1000 AT5G40 AACTCGGAAATTGATAGTACCAGATGTGAGAATATTGTTGACGAGGTGATGAATCTGAATCTGCACGCTACAACGTCCGTTTATGTTCCGGAGCAAGCAG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04D AACTCGGAAATTGATAGTACCAGATGTGAGAATATTGTTGACGAGGTGATGAATCTGAATCTGCACGCTACAACGTCCGTTTATGTTCCGGAGCAAGCAG 910 920 930 940 950 960 970 980 990 1000 1010 1020 1030 1040 1050 1060 1070 AT5G40 TGTTGACATGGGGTTATGATTTTACAAAATGTTATGAACCCATGGATGATACATGGATGCTCATGAATGGTTGGAAT ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04D TGTTGACATGGGGTTATGATTTTACAAAATGTTATGAACCCATGGATGATACATGGATGCTCATGAATGGTTGGAAT 1010 1020 1030 1040 1050 1060 1070 10 20 30 40 50 60 70 80 90 100 AT5G40 MYHQNLISSTPNQNSNPHDWDIQNPLFSIHPSAEIPSKYPFMGITSCPNTNVFEEFQYKITNDQNFPTTYNTPFPVISEGISYNMHDVQENTMCGYTAHN :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04D MYHQNLISSTPNQNSNPHDWDIQNPLFSIHPSAEIPSKYPFMGITSCPNTNVFEEFQYKITNDQNFPTTYNTPFPVISEGISYNMHDVQENTMCGYTAHN 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT5G40 QGLIIGCHEPVLVHAVVESQQFNVPQSEDINLVSQSERVTEDKVMFKTDHKKKDIIGKGQWTPTEDELLVRMVKSKGTKNWTSIAKMFQGRVGKQCRERW :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04D QGLIIGCHEPVLVHAVVESQQFNVPQSEDINLVSQSERVTEDKVMFKTDHKKKDIIGKGQWTPTEDELLVRMVKSKGTKNWTSIAKMFQGRVGKQCRERW 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT5G40 HNHLRPNIKKNDWSEEEDQILIEVHKIVGNKWTEIAKRLPGRSENIVKNHWNATKRRLHSVRTKRSDAFSPRNNALENYIRSITINNNALMNREVDSITA ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04D RNHLRPNIKKNDWSEEEDQILIEVHKIVGNKWTEIAKRLPGRSENIVKNHWNATKRRLHSVRTKRSDAFSPRNNALENYIRSITINNNALMNREVDSITA 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 AT5G40 NSEIDSTRCENIVDEVMNLNLHATTSVYVPEQAVLTWGYDFTKCYEPMDDTWMLMNGWN ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04D NSEIDSTRCENIVDEVMNLNLHATTSVYVPEQAVLTWGYDFTKCYEPMDDTWMLMNGWN 310 320 330 340 350

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