◆◆◆IE_04D04 detail◆◆◆

Code nameIE_04D04
LocusAT5G15480
TemplateIT_04D04 (HR2302)
Donor VectorpDONR207
Forward primerIPF_04D04 (ggggacaagtttgtacaaaaaagcaggcttcATGGAAGACGAACATCAAGATCTCCATA)
Reverse primerIPR_04D04 (ggggaccactttgtacaagaaagctgggttCAGAGTCATCCTGGGTTCAACTGATTCCTC)
Alignment with template seq.1stアミノ酸配列100%一致2nd-
Alignment with TAIR7CDSアミノ酸配列100%一致-
comment
sequence 
>IE_04D04_1206bp
ATGGAAGACGAACATCAAGATCTCCATAAACCCATTAATGGAGCTTTGCGAGACCTCAAGATTACTCGGTCACAGAAAGAAACAGAAAAGTCTACGAACC
AACAGCAAGATGTTACTTGTTACTATGGTCTAAGGGAAAACTCGAAGAAGAAAACCCAGGAATCTCCGGAACCAATGAAGAAGATTTTGTTTCGATGCGA
AGAATGTGGAAAAGGGTTTCGGTACGAGAAATATTTTAAGAATCATCGCTCGATGATGCATTTATCGCCGAACGAGAAGGTTTGTGAAGAATCCTTGATG
ACTCTGTCTCGTAGCCTTGGGTTTGTGAAGAAGAAGAAAAGATCAAGACTTGGTAGGTCTGGGAAGACTTTATTTACTACGTTTCTTGAACCGAGTTCTA
TTTTTGATGCGACTGATGAAGAATTAGAAGTGGCGGATTGTTTGATTCTATTGTCTAAGAGTGCTCCCAAGGTTGTAGACGAATTGAAAAGTCTTTCTGA
GGCAGTACGTGTTACTCCTGAAACACCTGAAAGTAGCTATGATTTGGGTTGTTTGCTCAACAAGAAACCGAGAAAAGGTGGTGAATTGGAATCTGGGGTT
TTAAGTAATGAGCAAAGACTTATGGAAGAAGGGTTTAGTAGTTATGGAACATCGAAAGAACCAGCTAGCTTCTTGAGAGACGAAAACAGATTGGATCAGC
AGAAACGGAGAAAAGATGGTGAATTTGAATCCGGACTTTTGAGTAATGAGCAAAGACTGCTAGAAGAAGAGATTACTACTCCTGTGACATTCAAAGGTCC
AGCGAGTTCCTTGAGACACAAGTGTGCTTTGGATCGAAATGGAGGTGAATTTGGTCCTGAGTTTTTGAGTAATGAGCAAACACTGATGGAAGAAACATGG
AAAGAACCAGTGAGTTTCTTAGAAGATAAGCATGAATTTGATCAGCGGAAAATGCGAGAAGCTGGCGACTTTGAATCTAGGTTTTACAGAATTGAGCTTG
GAGTAGGAGCTATGGAGTGTACTTCTTCAGATACTGATATGCTCACGCAATCTGATAAGAAGAACGTTGAGCATCGATGCAGGTTGTGCAACAAGATATT
CTCGTCTTATCAAGCTCTAGGGGGTCATCAGACGTTTCATCGGATGAGCAAATGTAAGAACAAGAAGAATGGCATAGAGGAATCAGTTGAACCCAGGATG
ACTCTG

>IE_04D04_translated
MEDEHQDLHKPINGALRDLKITRSQKETEKSTNQQQDVTCYYGLRENSKKKTQESPEPMKKILFRCEECGKGFRYEKYFKNHRSMMHLSPNEKVCEESLM
TLSRSLGFVKKKKRSRLGRSGKTLFTTFLEPSSIFDATDEELEVADCLILLSKSAPKVVDELKSLSEAVRVTPETPESSYDLGCLLNKKPRKGGELESGV
LSNEQRLMEEGFSSYGTSKEPASFLRDENRLDQQKRRKDGEFESGLLSNEQRLLEEEITTPVTFKGPASSLRHKCALDRNGGEFGPEFLSNEQTLMEETW
KEPVSFLEDKHEFDQRKMREAGDFESRFYRIELGVGAMECTSSDTDMLTQSDKKNVEHRCRLCNKIFSSYQALGGHQTFHRMSKCKNKKNGIEESVEPRM
TL
Alignment ◆CDS（TAIRver.9）_without_stop_codonとのalignment◆

100%一致しました。

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