◆◆◆IE_04D05 detail◆◆◆

Code name	IE_04D05
Locus	AT4G33880
Template	IT_04D05 (HR2394)
Donor Vector	pDONR207
Forward primer	IPF_04D05 (ggggacaagtttgtacaaaaaagcaggcttcATGGAAGCCATGGGAGAATGGAGCAACA)
Reverse primer	IPR_04D05 (ggggaccactttgtacaagaaagctgggttTCTCGGTGAGCTGAGACCAATGTCCATCCC)
Alignment with template seq.	1st	アミノ酸配列100%一致	2nd	-
Alignment with TAIR7CDS	1st	アミノ酸配列99.10%一致	2nd	-
comment	1st:Templateの配列とは一致
sequence	>IE_04D05_1056bp ATGGAAGCCATGGGAGAATGGAGCAACAACCTCGGAGGAATGTACACTTATGCAACCGAGGAAGCCGATTTCATGAACCAGCTTCTCGCCTCTTATGATC ATCCTGGCACCGGCTCATCCTCCGGCGCAGCAGCCAGTGGTGACCACCAAGGCTTGTATTGGAACCTTGGTTCTCATCACAACCACCTTAGCCTCGTGTC TGAAGCCGGTAGCTTCTGTTTCTCTCAAGAGAGCAGCAGCTACAGCGCTGGGAACAGCGGCTATTACACCGTTGTTCCACCCACGGTTGAAGAGAACCAA AATGAGACAATGGACTTTGGGATGGAAGATGTGACCATCAACACAAACTCATACCTTGTTGGTGAGGAGACAAGTGAGTGTGACGTTGAGAAATACTCTT CTGGAAAGACTCTTATGCCTTTGGAAACCGTAGTGGAGAACCACGATGACGAGGAAAGCTTGTTGCAATCTGAGATCTCTGTGACTACTACAAAATCTCT CACCGGCTCCAAAAAGAGATCCCGTGCCACATCTACTGATAAAAACAAGAGAGCAAGAGTGAATAAGAGGGCCCAGAAGAACGTAGAGATGAGTGGGGAT AACAATGAAGGGGAAGAGGAAGAAGGAGAGACGAAGTTGAAGAAAAGAAAGAATGGGGCAATGATTAGTAGACAGAACTCAAGCACCACTTTCTGTACGG AGGAAGATTCAAACTGCGCTGATCAAGACGGTGGAGGAGAAGACTCATCCTCTAAGGAAGATGATGCCTCAAAGGCCCTCAACCTCAATGGTAAAACAAG AGCCAGTCGTGGTGCAGCCACCGATCCTCAAAGCCTCTATGCAAGGAAAAGAAGAGAAAGGATTAACGAGAGACTAAGGATTTTACAAAATCTCGTCCCC AATGGAACAAAGGTCGATATTAGTACGATGCTTGAGGAAGCAGTTCATTACGTCAAATTTTTGCAGCTCCAAATTAAGTTATTGAGCTCTGATGATCTAT GGATGTATGCGCCGATTGCTTTCAATGGGATGGACATTGGTCTCAGCTCACCGAGA >IE_04D05_translated MEAMGEWSNNLGGMYTYATEEADFMNQLLASYDHPGTGSSSGAAASGDHQGLYWNLGSHHNHLSLVSEAGSFCFSQESSSYSAGNSGYYTVVPPTVEENQ NETMDFGMEDVTINTNSYLVGEETSECDVEKYSSGKTLMPLETVVENHDDEESLLQSEISVTTTKSLTGSKKRSRATSTDKNKRARVNKRAQKNVEMSGD NNEGEEEEGETKLKKRKNGAMISRQNSSTTFCTEEDSNCADQDGGGEDSSSKEDDASKALNLNGKTRASRGAATDPQSLYARKRRERINERLRILQNLVP NGTKVDISTMLEEAVHYVKFLQLQIKLLSSDDLWMYAPIAFNGMDIGLSSPR
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 1049/1056 (99.3%)（アミノ酸配列で349/352 (99.1%)）一致しました。 10 20 30 40 50 60 70 80 90 100 AT4G33 ATGGAAGCCATGGGAGAATGGAGCAACAACCTCGGAGGAATGTACACTTATGCAACCGAGGAAGCCGATTTCATGAACCAGCTTCTCGCCTCTTATGATC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04D ATGGAAGCCATGGGAGAATGGAGCAACAACCTCGGAGGAATGTACACTTATGCAACCGAGGAAGCCGATTTCATGAACCAGCTTCTCGCCTCTTATGATC 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT4G33 ATCCTGGCACCGGCTCATCCTCCGGCGCAGCAGCCAGTGGTGACCACCAAGGCTTGTATTGGAACCTTGGTTCTCATCACAACCACCTTAGCCTCGTGTC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04D ATCCTGGCACCGGCTCATCCTCCGGCGCAGCAGCCAGTGGTGACCACCAAGGCTTGTATTGGAACCTTGGTTCTCATCACAACCACCTTAGCCTCGTGTC 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT4G33 TGAAGCCGGTAGCTTCTGTTTCTCTCAAGAGAGCAGCAGCTACAGCGCTGGGAACAGCGGATATTACACCGTTGTTCCACCCACGGTTGAAGAGAACCAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: ::::::::::::::::::::::::::::::::::::::: IE_04D TGAAGCCGGTAGCTTCTGTTTCTCTCAAGAGAGCAGCAGCTACAGCGCTGGGAACAGCGGCTATTACACCGTTGTTCCACCCACGGTTGAAGAGAACCAA 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 AT4G33 AATGAGACAATGGACTTTGGGATGGAAGATGTGACCATCAATACAAACTCATACCTTGTTGGTGAGGAGACAAGTGAGTGTGACGTTGAGAAATACTCTT ::::::::::::::::::::::::::::::::::::::::: :::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04D AATGAGACAATGGACTTTGGGATGGAAGATGTGACCATCAACACAAACTCATACCTTGTTGGTGAGGAGACAAGTGAGTGTGACGTTGAGAAATACTCTT 310 320 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 480 490 500 AT4G33 CTGGAAAGACTCTTATGCCTTTGGAAACCGTAGTGGAGAACCACGATGACGAGGAAAGCTTGTTGCAATCTGAGATCTCTGTGACTACTACAAAATCTCT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04D CTGGAAAGACTCTTATGCCTTTGGAAACCGTAGTGGAGAACCACGATGACGAGGAAAGCTTGTTGCAATCTGAGATCTCTGTGACTACTACAAAATCTCT 410 420 430 440 450 460 470 480 490 500 510 520 530 540 550 560 570 580 590 600 AT4G33 CACCGGCTCCAAAAAGAGATCCCGTGCCACATCTACTGATAAAAACAAGAGAGCAAGAGTGAATAAGAGGGCCCAGAAGAACGTAGAGATGAGTGGGGAT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04D CACCGGCTCCAAAAAGAGATCCCGTGCCACATCTACTGATAAAAACAAGAGAGCAAGAGTGAATAAGAGGGCCCAGAAGAACGTAGAGATGAGTGGGGAT 510 520 530 540 550 560 570 580 590 600 610 620 630 640 650 660 670 680 690 700 AT4G33 AACAATGAAGGAGAAGAGGAAGAAGGAGAGACGAAGTTGAAGAAAAGAAAGAATGGGGCAATGATGAGTAGACAGAACTCAAGCACCACTTTCTGTACGG ::::::::::: ::::::::::::::::::::::::::::::::::::::::::::::::::::: :::::::::::::::::::::::::::::::::: IE_04D AACAATGAAGGGGAAGAGGAAGAAGGAGAGACGAAGTTGAAGAAAAGAAAGAATGGGGCAATGATTAGTAGACAGAACTCAAGCACCACTTTCTGTACGG 610 620 630 640 650 660 670 680 690 700 710 720 730 740 750 760 770 780 790 800 AT4G33 AGGAAGAATCAAACTGCGCTGATCAAGACGGTGGAGGAGAAGACTCATCCTCTAAGGAAGATGATCCCTCAAAGGCCCTCAACCTCAATGGTAAAACAAG ::::::: ::::::::::::::::::::::::::::::::::::::::::::::::::::::::: :::::::::::::::::::::::::::::::::: IE_04D AGGAAGATTCAAACTGCGCTGATCAAGACGGTGGAGGAGAAGACTCATCCTCTAAGGAAGATGATGCCTCAAAGGCCCTCAACCTCAATGGTAAAACAAG 710 720 730 740 750 760 770 780 790 800 810 820 830 840 850 860 870 880 890 900 AT4G33 AGCCAGTCGTGGTGCAGCCACCGATCCTCAAAGCCTCTATGCAAGGAAAAGAAGAGAAAGGATTAACGAGAGACTAAGGATTTTACAAAATCTCGTCCCC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04D AGCCAGTCGTGGTGCAGCCACCGATCCTCAAAGCCTCTATGCAAGGAAAAGAAGAGAAAGGATTAACGAGAGACTAAGGATTTTACAAAATCTCGTCCCC 810 820 830 840 850 860 870 880 890 900 910 920 930 940 950 960 970 980 990 1000 AT4G33 AATGGAACAAAGGTCGATATTAGTACAATGCTTGAGGAAGCAGTTCATTACGTCAAATTTTTGCAGCTCCAAATTAAGTTATTGAGCTCTGATGATCTAT :::::::::::::::::::::::::: ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04D AATGGAACAAAGGTCGATATTAGTACGATGCTTGAGGAAGCAGTTCATTACGTCAAATTTTTGCAGCTCCAAATTAAGTTATTGAGCTCTGATGATCTAT 910 920 930 940 950 960 970 980 990 1000 1010 1020 1030 1040 1050 AT4G33 GGATGTATGCGCCGATTGCTTTCAATGGGATGGACATTGGTCTCAGCTCACCGAGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04D GGATGTATGCGCCGATTGCTTTCAATGGGATGGACATTGGTCTCAGCTCACCGAGA 1010 1020 1030 1040 1050 10 20 30 40 50 60 70 80 90 100 AT4G33 MEAMGEWSNNLGGMYTYATEEADFMNQLLASYDHPGTGSSSGAAASGDHQGLYWNLGSHHNHLSLVSEAGSFCFSQESSSYSAGNSGYYTVVPPTVEENQ :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04D MEAMGEWSNNLGGMYTYATEEADFMNQLLASYDHPGTGSSSGAAASGDHQGLYWNLGSHHNHLSLVSEAGSFCFSQESSSYSAGNSGYYTVVPPTVEENQ 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT4G33 NETMDFGMEDVTINTNSYLVGEETSECDVEKYSSGKTLMPLETVVENHDDEESLLQSEISVTTTKSLTGSKKRSRATSTDKNKRARVNKRAQKNVEMSGD :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04D NETMDFGMEDVTINTNSYLVGEETSECDVEKYSSGKTLMPLETVVENHDDEESLLQSEISVTTTKSLTGSKKRSRATSTDKNKRARVNKRAQKNVEMSGD 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT4G33 NNEGEEEEGETKLKKRKNGAMMSRQNSSTTFCTEEESNCADQDGGGEDSSSKEDDPSKALNLNGKTRASRGAATDPQSLYARKRRERINERLRILQNLVP :::::::::::::::::::::.:::::::::::::.::::::::::::::::::: :::::::::::::::::::::::::::::::::::::::::::: IE_04D NNEGEEEEGETKLKKRKNGAMISRQNSSTTFCTEEDSNCADQDGGGEDSSSKEDDASKALNLNGKTRASRGAATDPQSLYARKRRERINERLRILQNLVP 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 AT4G33 NGTKVDISTMLEEAVHYVKFLQLQIKLLSSDDLWMYAPIAFNGMDIGLSSPR :::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04D NGTKVDISTMLEEAVHYVKFLQLQIKLLSSDDLWMYAPIAFNGMDIGLSSPR 310 320 330 340 350

このページのTOPに戻る