◆◆◆IE_04D06 detail◆◆◆

Code name	IE_04D06
Locus	AT1G69170
Template	IT_04D06 (HR2430)
Donor Vector	pDONR207
Forward primer	IPF_04D06 (ggggacaagtttgtacaaaaaagcaggcttcATGGATTCTTGGAGCTACGGGAGAAGCG)
Reverse primer	IPR_04D06 (ggggaccactttgtacaagaaagctgggttGGAGCCAGGGAAATAAAGCTCATTATATTC)
Alignment with template seq.	1st	アミノ酸配列100%一致	2nd	-
Alignment with TAIR7CDS	1st	アミノ酸配列100%一致	2nd	-
comment	1st:Templateの配列とは一致
sequence	>IE_04D06_1215bp ATGGATTCTTGGAGCTACGGGAGAAGCGTTTTCATGTCAAATGAAACTCTTTTACCTTGTGATACTTTTGCTAAGAATAGAAGATTTGAACAGAGACTCT CTAATAATGATGATGTGTTGATTTCTGACATGGCTGGTAACTCCAATGGATTCAGTGCTGTTTCTATTACTAAAGTTGTTCCTGAAGAGGAAGATGAGGA GAACATATCTTCTTCTTCAAAGTTCTCTAGTCAGGAATTGAATAGGATAGATTTCAAACTTAGGAGCTTTTTGGATTTAGGAAATGATGATGATGATACA TCCTCTAGAGGTTTTGCTCTGCCATCTAAAAAGTCTCGAGCTTCAAACTTGTGCTCTCAGAATCCCTTGTGTCAAGTTTATGGGTGTAGTAAGGATCTGA GCTCTTCGAAAGATTACCACAAAAGGCATAGAGTTTGCGAGGCTCATTCGAAAACTTCTGTGGTCATAGTTAATGGTCTTGAACAGAGGTTTTGTCAACA GTGCAGCAGGTTTCATTTCCTCTCAGAGTTTGATGATGGCAAAAGAAGTTGCAGAAGGCGATTAGCCGGTCACAATGAACGAAGAAGGAAACCTGCATTC TATTTCCTACCGGGTAAGCGCCATAAGCTTCTTCGCACCTCTCAAGATGTAGTAGGCAACAAGTTTCTGGAGAATTCATCATTGGTATTGCCAGAGTCAT TTCCTGGTAGTCTCTTATACAGAGTAATAGATGAAGACGACCACCGTACAAGTAGACTCGTGAGTTTCAAAGATGAACCTACTTGTTCCATGTTTCCTAC TAATGAGCAAAACAGCAGCAGAACTTATGAATCTAAACCAGCAATTTATTCCACGGAAGTATCCTCTATTTGGGACTTACATGAGACGGCGGCATCACGC TCTACTCGTGCTCTCTCTCTTCTGTCAGCTCAGTCCCAACAACACTTGTCTAAGTTTCCAAACACAACGTTCTCAATCACCCAACCCAACCAAAATCTCA ATCACTCATCATCAACAGACTATCATCAGATGGAACAACCGTTGTGGATCGATCCTGGCAAGACCAATTCTGCTGGTTCTAGTTCTTGTAAAGGAAAAGG AACATCCACGGTTGATCTACTGCAACTGTCATCACATCTTCAAAGAATCGAGCAACAGAGGAATTACACTGGTGATGTGAAGCAGGAATATAATGAGCTT TATTTCCCTGGCTCC >IE_04D06_translated MDSWSYGRSVFMSNETLLPCDTFAKNRRFEQRLSNNDDVLISDMAGNSNGFSAVSITKVVPEEEDEENISSSSKFSSQELNRIDFKLRSFLDLGNDDDDT SSRGFALPSKKSRASNLCSQNPLCQVYGCSKDLSSSKDYHKRHRVCEAHSKTSVVIVNGLEQRFCQQCSRFHFLSEFDDGKRSCRRRLAGHNERRRKPAF YFLPGKRHKLLRTSQDVVGNKFLENSSLVLPESFPGSLLYRVIDEDDHRTSRLVSFKDEPTCSMFPTNEQNSSRTYESKPAIYSTEVSSIWDLHETAASR STRALSLLSAQSQQHLSKFPNTTFSITQPNQNLNHSSSTDYHQMEQPLWIDPGKTNSAGSSSCKGKGTSTVDLLQLSSHLQRIEQQRNYTGDVKQEYNEL YFPGS
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 1213/1215 (99.8%)（アミノ酸配列で100%）一致しました。 10 20 30 40 50 60 70 80 90 100 AT1G69 ATGGATTCTTGGAGCTACGGGAGAAGCGTTTTCATGTCAAATGAAACTCTTTTACCTTGTGATACTTTTGCTAAGAATAGAAGATTTGAACAGAGACTCT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04D ATGGATTCTTGGAGCTACGGGAGAAGCGTTTTCATGTCAAATGAAACTCTTTTACCTTGTGATACTTTTGCTAAGAATAGAAGATTTGAACAGAGACTCT 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT1G69 CTAATAATGATGATGTGTTGATTTCTGACATGGCTGGTAACTCCAATGGATTCAGTGCTGTTTCTATTACTAAAGTTGTTCCTGAAGAGGAAGATGAGGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04D CTAATAATGATGATGTGTTGATTTCTGACATGGCTGGTAACTCCAATGGATTCAGTGCTGTTTCTATTACTAAAGTTGTTCCTGAAGAGGAAGATGAGGA 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT1G69 GAACATATCTTCTTCTTCAAAGTTCTCTAGTCAGGAATTGAATAGGATAGATTTCAAACTTAGGAGCTTTTTGGATTTAGGAAATGATGATGATGATACA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04D GAACATATCTTCTTCTTCAAAGTTCTCTAGTCAGGAATTGAATAGGATAGATTTCAAACTTAGGAGCTTTTTGGATTTAGGAAATGATGATGATGATACA 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 AT1G69 TCCTCTAGAGGTTTTGCTCTGCCATCTAAAAAGTCTCGAGCTTCAAACTTGTGCTCTCAGAATCCCTTGTGTCAAGTTTATGGGTGTAGTAAGGATCTGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04D TCCTCTAGAGGTTTTGCTCTGCCATCTAAAAAGTCTCGAGCTTCAAACTTGTGCTCTCAGAATCCCTTGTGTCAAGTTTATGGGTGTAGTAAGGATCTGA 310 320 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 480 490 500 AT1G69 GCTCTTCGAAAGATTACCACAAAAGGCATAGAGTTTGCGAGGCTCATTCGAAAACTTCTGTGGTCATAGTTAATGGTCTTGAACAGAGGTTTTGTCAACA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04D GCTCTTCGAAAGATTACCACAAAAGGCATAGAGTTTGCGAGGCTCATTCGAAAACTTCTGTGGTCATAGTTAATGGTCTTGAACAGAGGTTTTGTCAACA 410 420 430 440 450 460 470 480 490 500 510 520 530 540 550 560 570 580 590 600 AT1G69 GTGCAGCAGGTTTCATTTCCTCTCAGAGTTTGATGATGGCAAAAGAAGTTGCAGAAGGCGATTAGCCGGTCACAATGAACGAAGAAGGAAACCTGCATTC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04D GTGCAGCAGGTTTCATTTCCTCTCAGAGTTTGATGATGGCAAAAGAAGTTGCAGAAGGCGATTAGCCGGTCACAATGAACGAAGAAGGAAACCTGCATTC 510 520 530 540 550 560 570 580 590 600 610 620 630 640 650 660 670 680 690 700 AT1G69 TATTTCCTACCGGGTAAGCGCCATAAGCTTCTTCGCACCTCTCAAGATGTAGTAGGCAACAAGTTTCTGGAGAATTCATCATTGGTATTGCCAGAGTCAT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04D TATTTCCTACCGGGTAAGCGCCATAAGCTTCTTCGCACCTCTCAAGATGTAGTAGGCAACAAGTTTCTGGAGAATTCATCATTGGTATTGCCAGAGTCAT 610 620 630 640 650 660 670 680 690 700 710 720 730 740 750 760 770 780 790 800 AT1G69 TTCCTGGTAGTCTCTTATACAGAGTAATAGATGAAGACGACCACCGTACAAGTAGACTCGTGAGTTTCAAAGATGAACCTACTTGTTCCATGTTTCCTAC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04D TTCCTGGTAGTCTCTTATACAGAGTAATAGATGAAGACGACCACCGTACAAGTAGACTCGTGAGTTTCAAAGATGAACCTACTTGTTCCATGTTTCCTAC 710 720 730 740 750 760 770 780 790 800 810 820 830 840 850 860 870 880 890 900 AT1G69 TAATGAGCAAAACAGCAGCAGAACTTATGAATCTAAACCAGCAATTTATTCCACGGAAGTATCCTCCATTTGGGACTTACATGAGACGGCGGCATCACGC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: ::::::::::::::::::::::::::::::::: IE_04D TAATGAGCAAAACAGCAGCAGAACTTATGAATCTAAACCAGCAATTTATTCCACGGAAGTATCCTCTATTTGGGACTTACATGAGACGGCGGCATCACGC 810 820 830 840 850 860 870 880 890 900 910 920 930 940 950 960 970 980 990 1000 AT1G69 TCTACTCGTGCTCTCTCTCTTCTGTCAGCTCAGTCCCAACAACACTTGTCTAAGTTTCCAAACACAACGTTCTCAATCACCCAACCCAACCAAAATCTCA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04D TCTACTCGTGCTCTCTCTCTTCTGTCAGCTCAGTCCCAACAACACTTGTCTAAGTTTCCAAACACAACGTTCTCAATCACCCAACCCAACCAAAATCTCA 910 920 930 940 950 960 970 980 990 1000 1010 1020 1030 1040 1050 1060 1070 1080 1090 1100 AT1G69 ATCACTCATCATCAACTGACTATCATCAGATGGAACAACCGTTGTGGATCGATCCTGGCAAGACCAATTCTGCTGGTTCTAGTTCTTGTAAAGGAAAAGG :::::::::::::::: ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04D ATCACTCATCATCAACAGACTATCATCAGATGGAACAACCGTTGTGGATCGATCCTGGCAAGACCAATTCTGCTGGTTCTAGTTCTTGTAAAGGAAAAGG 1010 1020 1030 1040 1050 1060 1070 1080 1090 1100 1110 1120 1130 1140 1150 1160 1170 1180 1190 1200 AT1G69 AACATCCACGGTTGATCTACTGCAACTGTCATCACATCTTCAAAGAATCGAGCAACAGAGGAATTACACTGGTGATGTGAAGCAGGAATATAATGAGCTT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04D AACATCCACGGTTGATCTACTGCAACTGTCATCACATCTTCAAAGAATCGAGCAACAGAGGAATTACACTGGTGATGTGAAGCAGGAATATAATGAGCTT 1110 1120 1130 1140 1150 1160 1170 1180 1190 1200 1210 AT1G69 TATTTCCCTGGCTCC ::::::::::::::: IE_04D TATTTCCCTGGCTCC 1210 10 20 30 40 50 60 70 80 90 100 AT1G69 MDSWSYGRSVFMSNETLLPCDTFAKNRRFEQRLSNNDDVLISDMAGNSNGFSAVSITKVVPEEEDEENISSSSKFSSQELNRIDFKLRSFLDLGNDDDDT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04D MDSWSYGRSVFMSNETLLPCDTFAKNRRFEQRLSNNDDVLISDMAGNSNGFSAVSITKVVPEEEDEENISSSSKFSSQELNRIDFKLRSFLDLGNDDDDT 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT1G69 SSRGFALPSKKSRASNLCSQNPLCQVYGCSKDLSSSKDYHKRHRVCEAHSKTSVVIVNGLEQRFCQQCSRFHFLSEFDDGKRSCRRRLAGHNERRRKPAF :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04D SSRGFALPSKKSRASNLCSQNPLCQVYGCSKDLSSSKDYHKRHRVCEAHSKTSVVIVNGLEQRFCQQCSRFHFLSEFDDGKRSCRRRLAGHNERRRKPAF 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT1G69 YFLPGKRHKLLRTSQDVVGNKFLENSSLVLPESFPGSLLYRVIDEDDHRTSRLVSFKDEPTCSMFPTNEQNSSRTYESKPAIYSTEVSSIWDLHETAASR :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04D YFLPGKRHKLLRTSQDVVGNKFLENSSLVLPESFPGSLLYRVIDEDDHRTSRLVSFKDEPTCSMFPTNEQNSSRTYESKPAIYSTEVSSIWDLHETAASR 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 AT1G69 STRALSLLSAQSQQHLSKFPNTTFSITQPNQNLNHSSSTDYHQMEQPLWIDPGKTNSAGSSSCKGKGTSTVDLLQLSSHLQRIEQQRNYTGDVKQEYNEL :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04D STRALSLLSAQSQQHLSKFPNTTFSITQPNQNLNHSSSTDYHQMEQPLWIDPGKTNSAGSSSCKGKGTSTVDLLQLSSHLQRIEQQRNYTGDVKQEYNEL 310 320 330 340 350 360 370 380 390 400 AT1G69 YFPGS ::::: IE_04D YFPGS

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