◆◆◆IE_04D12 detail◆◆◆

Code nameIE_04D12
LocusAT3G21880
TemplateIT_04D12 (HR2648)
Donor VectorpDONR207
Forward primerIPF_04D12 (ggggacaagtttgtacaaaaaagcaggcttcATGGAGCCAAAGTGTGACCATTGTGCAA)
Reverse primerIPR_04D12 (ggggaccactttgtacaagaaagctgggttATTGTTCGTCGTCGGTGATGATGGA)
Alignment with template seq.1stアミノ酸配列100%一致2nd-
Alignment with TAIR7CDSアミノ酸配列100%一致-
comment
sequence 
>IE_04D12_1092bp
ATGGAGCCAAAGTGTGACCATTGTGCAACCTCGCAAGCATTGATTTACTGCAAATCCGATTTGGCTAAACTATGCCTAAACTGCGACGTCCATGTACACT
CTGCGAATCCTCTGTCTCACAGGCACATACGCTCTTTGATCTGCGAGAAATGCTTCTCACAGCCAGCCGCAATCCGCTGTCTCGACGAGAAGGTTTCATA
CTGCCAAGGATGTCATTGGCACGAAAGCAACTGTTCGGAGTTAGGACACAGAGTTCAGAGCTTGAATCCTTTCTCTGGTTGTCCTTCTCCTACAGATTTT
AACAGAATGTGGTCTTCAATTCTTGAACCTCCTGTCTCCGGCTTGCTTAGTCCGTTCGTTGGTTCTTTTCCCTTGAATGATCTCAACAATACCATGTTCG
ATACCGCTTATTCCATGGTACCACACAACATCAGTTACACTCAGAATTTCAGTGATAATTTGTCTTTCTTTTCCACGGAATCTAAGGGATATCCTGATAT
GGTTCTTAAACTTGAAGAAGGAGAAGAAGATCTATGTGAAGGGCTTAACTTGGATGATGCACCATTAAACTTTGACGTAGGAGATGATATTATCGGATGT
TCATCAGAAGTACACATAGAACCGGACCATACGGTACCAAATTGCCTATTGATTGACAAGACTAACACATCCAGCTTTACCGGCTCCAACTTTACTGTAG
ACAAGGCTCTAGAGGCATCGCCACCAGGTCAGCAAATGAACATCAACACTGGACTTCAGCTTCCTCTATCACCTGTCTTGTTCGGACAAATCCATCCATC
TCTTAACATCACTGGTGAAAACAATGCAGCTGATTACCAGGATTGTGGGATGTCTCCAGGGTTTATAATGAGTGAAGCGCCATGGGAAACGAATTTTGAA
GTTAGTTGTCCACAAGCAAGAAACGAGGCTAAGTTGAGATACAAGGAGAAGAAATTAAAGCGCTCTTTTGGGAAACAGATTCGATATGCATCTCGCAAAG
CAAGAGCTGACACGAGGAAAAGAGTGAAAGGAAGATTTGTGAAAGCTGGTGACAGTTATGATTATGATCCATCATCACCGACGACGAACAAT

>IE_04D12_translated
MEPKCDHCATSQALIYCKSDLAKLCLNCDVHVHSANPLSHRHIRSLICEKCFSQPAAIRCLDEKVSYCQGCHWHESNCSELGHRVQSLNPFSGCPSPTDF
NRMWSSILEPPVSGLLSPFVGSFPLNDLNNTMFDTAYSMVPHNISYTQNFSDNLSFFSTESKGYPDMVLKLEEGEEDLCEGLNLDDAPLNFDVGDDIIGC
SSEVHIEPDHTVPNCLLIDKTNTSSFTGSNFTVDKALEASPPGQQMNINTGLQLPLSPVLFGQIHPSLNITGENNAADYQDCGMSPGFIMSEAPWETNFE
VSCPQARNEAKLRYKEKKLKRSFGKQIRYASRKARADTRKRVKGRFVKAGDSYDYDPSSPTTNN
Alignment ◆CDS（TAIRver.9）_without_stop_codonとのalignment◆

100%一致しました。

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