◆◆◆IE_04E05 detail◆◆◆

Code nameIE_04E05
LocusAT5G38490
TemplateIT_04E05 (HR2682)
Donor VectorpDONR207
Forward primerIPF_04E05 (ggggacaagtttgtacaaaaaagcaggcttcATGAGTAGTATTATGTATCACGATAATC)
Reverse primerIPR_04E05 (ggggaccactttgtacaagaaagctgggttCTCTGTGTCAAGTGCAAAGCAGAGG)
Alignment with template seq.1stアミノ酸配列100%一致2nd-
Alignment with TAIR7CDSアミノ酸配列100%一致-
comment
sequence 
>IE_04E05_1092bp
ATGAGTAGTATTATGTATCACGATAATCATCTCACGGATTCGGGGAAGAATATGTCGTCGGGTTTAAGTTTACTGGCGTATGTGACGGTTATGGTTGCGG
AGGAAGAAGAACAACGTAGTCGTCTTCTTGCAGAGAAACGAGAAGAATCCAAGTGCAAGATGAGTTTCTTCTATCTTTTCCCGAGGAAAATCAAATCGTC
TTTGGCGAAGAGAAGATACACACAACAAAACCCTAATGGGGCTTCTACTTGTTCGTCATCGCTTCCCGATCTTAACCTAATCCCTACTGATTTTGAGACA
GACAACCTACAAAACCCTTGTTTTGATGAACCTATGGTGTGTGATGAAGAACAACGTTTAAAGAAAGGTAAATCCAAGATTGTCTACGATGAAGATTATG
ATGACGAGAGTGAAAAGAAACTCTTTGATAATCTTAATGGAGCTTCTACTTCTTCTTCATCGCTCCTGAACCTTCCTTGTCTTGAACCTTCGACAGAGAC
AAAAGACGTACCAAACCCTAATTACCAATCTTCGTCTCCGTCTTCATGCCTAACAGGGAAGACAAACCGCAAGAGGCGTGCCGTGGAGCAGAGGAAGAGT
GGTAAAGTCAAGAAAGTTAAGGTTTCTCCTTTGCCAAGGTTGTGTACAGAGATGCCTGAGTGGATTTTCCAAGTGATGAGATACATGAACGCTGATGCTG
AAACCCCGAGGCTGATATTTGAGAGGACTCTGTTTAAGAGTGATGTCAACTCAAACCTTAGTCGTCTCTTAATGCCTTTCCAGAAGCTAATCAGAAACGA
TTTCCTGACTCCTGCGGAGTGTAGAGCTATGCAGAAAGACGAAGACAATGATGAAGAAGACGACGAGAATATTGGTGTGGGAACGGTTCTTGTGAACCAA
AGATTTCAAAAGTGGGGTTTGCGTTTCAAGATATGGGCGATGGAGAAGGACTCTGGACATGGAACATTGAATTACACTTTGAATTGGGGTTGGAATGATG
TCGTCAAAAGTAGCAGCTTAAAAGTCGGCGACAAGATCAGCCTTTGGACTTTCAGGTGCCGTGGAGTCCTCTGCTTTGCACTTGACACAGAG

>IE_04E05_translated
MSSIMYHDNHLTDSGKNMSSGLSLLAYVTVMVAEEEEQRSRLLAEKREESKCKMSFFYLFPRKIKSSLAKRRYTQQNPNGASTCSSSLPDLNLIPTDFET
DNLQNPCFDEPMVCDEEQRLKKGKSKIVYDEDYDDESEKKLFDNLNGASTSSSSLLNLPCLEPSTETKDVPNPNYQSSSPSSCLTGKTNRKRRAVEQRKS
GKVKKVKVSPLPRLCTEMPEWIFQVMRYMNADAETPRLIFERTLFKSDVNSNLSRLLMPFQKLIRNDFLTPAECRAMQKDEDNDEEDDENIGVGTVLVNQ
RFQKWGLRFKIWAMEKDSGHGTLNYTLNWGWNDVVKSSSLKVGDKISLWTFRCRGVLCFALDTE
Alignment ◆CDS（TAIRver.9）_without_stop_codonとのalignment◆

100%一致しました。

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