◆◆◆IE_04E11 detail◆◆◆

Code nameIE_04E11
LocusAT4G02670
TemplateIT_04E11 (HR3281)
Donor VectorpDONR207
Forward primerIPF_04E11 (ggggacaagtttgtacaaaaaagcaggcttcATGTTTTCTTCTCATTCCTTGTCCTATA)
Reverse primerIPR_04E11 (ggggaccactttgtacaagaaagctgggttCATACGATGGGCCCCTGAGAATCCCAGATG)
Alignment with template seq.1stアミノ酸配列100%一致2nd-
Alignment with TAIR7CDSアミノ酸配列100%一致-
comment
sequence 
>IE_04E11_1206bp
ATGTTTTCTTCTCATTCCTTGTCCTATAAGCTAAGCTCTTTGTCTACTGAAGCAAGTGCCTCTTCTGGTAACAACACACTCAGTACCATCCAAGAATTCA
GTGGCTTTCACAATGTGATTTCTAGTGTTTGTACACACACAGAGACTCACAAGCCTAAGAAAAAGAGAGGCCTTCCTGGAAATCCTGATCCAGATGCAGA
AGTGATCGCATTATCACCAAAGACACTTCTTGCAACAAACAGATTCGTTTGTGAGATATGCAACAAAGGGTTTCAAAGAGATCAGAATCTACAGCTTCAC
AGGAGAGGCCACAATCTTCCATGGAAGCTGAAACAGAAGAACACCAAAGAACAACAAAAGAAGAAAGTCTACGTGTGCCCAGAGACAAACTGTGCTCATC
ACCATCCATCTAGAGCTCTTGGGGATCTCACAGGGATCAAGAAACACTTTTGCAGGAAACATGGAGAGAAGAAATGGAAATGTGAAAAGTGTTCAAAGTT
CTATGCTGTTCAATCTGATTGGAAAGCTCATACCAAGATCTGTGGTACTAGAGATTATAGATGTGACTGCGGCACTCTTTTCTCGAGGAAAGACACTTTC
ATAACGCATAGAGCGTTTTGTGATGCATTAGCAGAAGAAAGTGCAAGACTCCACTCAACTTCTTCCTCCAATCTCACAAACCCTAACCCTAATTTCCAAG
GCCATCATTTTATGTTCAATAAATCTTCCTCCTTGCTCTTCACGTCATCACCTCTTTTCATCGAACCATCACTTTCCACCGCCGCTCTTTCAACGCCTCC
AACGGCAGCTCTTTCCGCGACGGCTCTTCTCCAAAAGGCCACCTCTCTCAGCTCCACTACTTTCGGCGGCGGAGGACAGACTCGATCCATTGGTCATCAC
CGACATTTGACGAACGTCAACGAGTTTCTTGGTGTTGACCGAGTCATGATGACGTCAGCTTCTTCGTCTGAGTACGACCAGTTAGTTGTTGATGGGTTTA
CGTCCACGTGGCAGAAAGCTGACCGTTTGACCAGAGACTTTCTTGGACTCACGGGTCATGGGGGACACGTTAGCGTCAGACCTGGGGATATGCTAGAGTA
CGCGGGTGGAGTGGCGTTTCCTATGTCGGCGTACGATACCGAATCTCATGATCACTCGTTTCAGAAGGCTTACGATCATCTGGGATTCTCAGGGGCCCAT
CGTATG

>IE_04E11_translated
MFSSHSLSYKLSSLSTEASASSGNNTLSTIQEFSGFHNVISSVCTHTETHKPKKKRGLPGNPDPDAEVIALSPKTLLATNRFVCEICNKGFQRDQNLQLH
RRGHNLPWKLKQKNTKEQQKKKVYVCPETNCAHHHPSRALGDLTGIKKHFCRKHGEKKWKCEKCSKFYAVQSDWKAHTKICGTRDYRCDCGTLFSRKDTF
ITHRAFCDALAEESARLHSTSSSNLTNPNPNFQGHHFMFNKSSSLLFTSSPLFIEPSLSTAALSTPPTAALSATALLQKATSLSSTTFGGGGQTRSIGHH
RHLTNVNEFLGVDRVMMTSASSSEYDQLVVDGFTSTWQKADRLTRDFLGLTGHGGHVSVRPGDMLEYAGGVAFPMSAYDTESHDHSFQKAYDHLGFSGAH
RM
Alignment ◆CDS（TAIRver.9）_without_stop_codonとのalignment◆

100%一致しました。

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