◆◆◆IE_04E12（IE_09B01） detail◆◆◆

Code nameIE_04E12（IE_09B01）
LocusAT4G31640
TemplateIT_04E12 (HR3304)
Donor VectorpDONR207
Forward primerIPF_04E12 (ggggacaagtttgtacaaaaaagcaggcttcATGAAGAATCCGACACTCTTTGCTCCAA)
Reverse primerIPR_04E12 (ggggaccactttgtacaagaaagctgggttTAACTTAGTTTTCGTTTCCTGTAACAAAGC)
Alignment with template seq.1stアミノ酸配列100%一致2ndアミノ酸配列100%一致
Alignment with TAIR7CDSアミノ酸配列100%一致アミノ酸配列100%一致
comment1st:(Sequence解析まで行ったが、読み直す必要がある)→2nd(09B01):100%
sequence 
>IE_04E12_1056bp
ATGAAGAATCCGACACTCTTTGCTCCAAAAAGTCCACACTTTTTCCAGCCTATTCTTCCCGGTTTCAAGAGCCACATCAAAATCCCTGTGAAGTTCTTCT
CCAAGCATATAGAGGGAAAACACGAGGGAAATACTGTCTATTTGAGATCATATCCTTCGAGGAGAACGTGGAAAGTGAAGATGGAGGGCCATAAACTCAC
TGAAGGTTGGAAAGAGTTCGTGGAGGCACATGATCTTCGAGTCGGTGACTTCGTCGTCTTCAAACACAAAGGAGACATGTTGTTCCATGTGACTGCTATA
GGACCCAGTTGCTGTGAAGTCCAATACGCACCATCTCGTAGCCACGACAGAAACGAAGAGAGTGATGAAATTGGGGAATCTTCAAGAAATGAGAAAATTA
TTGAGGAGAATGTGAAAACAGAACCTGATCAGTTTTCACCAGATCTAACATGTTTTAGCCAATCTGTGACCGCTTCAAACCTAACGAGAGATTTAGTGGG
TATTCCCAGAGATTTCGCAAAGCGATATGGTTTGAACATAGGTAGGCATGAGATAGTTTTGATGGACGAAGAAGGAAATACATGGGAATCAGAAGTTAAA
AGTTACAAGTCTGGTCGGGTTTTTATAGCTGGAGGTTGGACAAGTCTCTGCACTGCAAATAAATTAGAAGTTGGAGATTCTTGTACATTTAAACTGCTCC
GCAACCCGAAAATTCCCGTGTTTCGGCTCTGCAGCCGCCCAAAGGCTGGAGCAGAGGCTCGGCCACACAAGAGATCTCGAGTCCAAAGTAGCAGCGGGGG
GAGTAGACAGAAGCATGACGTGAGGCAGAAGTTAGATGAAGAAGGAGGGCCATCTCGTTGCACTCGGTCATCTAACAAGGCTACTGGTGATCAAGAAAAG
TTGCAGCAAACGCAATCTTGCTTTATCTTTGATCATGTGGCTAAGGTGAAACAGAGCGTTGAAGATACTTTAAATAGTATTAGACGGTTCCGGGCTAAAC
TCGAGACAAAGGAACATAATTTGGAAGCTTTGTTACAGGAAACGAAAACTAAGTTA

>IE_04E12_translated
MKNPTLFAPKSPHFFQPILPGFKSHIKIPVKFFSKHIEGKHEGNTVYLRSYPSRRTWKVKMEGHKLTEGWKEFVEAHDLRVGDFVVFKHKGDMLFHVTAI
GPSCCEVQYAPSRSHDRNEESDEIGESSRNEKIIEENVKTEPDQFSPDLTCFSQSVTASNLTRDLVGIPRDFAKRYGLNIGRHEIVLMDEEGNTWESEVK
SYKSGRVFIAGGWTSLCTANKLEVGDSCTFKLLRNPKIPVFRLCSRPKAGAEARPHKRSRVQSSSGGSRQKHDVRQKLDEEGGPSRCTRSSNKATGDQEK
LQQTQSCFIFDHVAKVKQSVEDTLNSIRRFRAKLETKEHNLEALLQETKTKL
Alignment ◆CDS（TAIRver.9）_without_stop_codonとのalignment◆

100%一致しました。

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