◆◆◆IE_04F01 detail◆◆◆

Code nameIE_04F01
LocusAT5G53660
TemplateIT_04F01 (HR3351)
Donor VectorpDONR207
Forward primerIPF_04F01 (ggggacaagtttgtacaaaaaagcaggcttcATGGACTTTCTCAAAGTTTCAGACAAGA)
Reverse primerIPR_04F01 (ggggaccactttgtacaagaaagctgggttAACCTGGCTGCTTTCGTCGGACACTGAACT)
Alignment with template seq.1stアミノ酸配列100%一致2nd-
Alignment with TAIR7CDSアミノ酸配列100%一致-
comment
sequence 
>IE_04F01_1095bp
ATGGACTTTCTCAAAGTTTCAGACAAGACAACAATTCCATATAGAAGTGATTCTTTGTTTAGTTTGAATCAGCAACAATACAAAGAGTCTTCTTTTGGAT
TCAGAGACATGGAGATTCATCCGCATCCTACTCCATATGCAGGAAATGGACTTTTGGGTTGTTATTACTATTACCCTTTCACAAACGCACAATTGAAGGA
GCTTGAGAGACAAGCAATGATCTACAAGTACATGATCGCATCTATTCCTGTTCCTTTCGATCTACTTGTTTCTTCACCATCCTCTGCCTCTCCTTGTAAC
AATAAAAACATCGCCGGAGATTTAGAGCCGGGAAGATGCCGGAGAACAGACGGAAAGAAATGGAGATGCGCGAAAGAAGTCGTCTCTAATCACAAATACT
GTGAGAAACACTTACACAGAGGTCGTCCTCGTTCAAGAAAGCATGTGGAACCTCCTTATTCTCGCCCTAACAACAATGGTGGTTCTGTGAAAAACAGAGA
TCTCAAAAAGCTTCCTCAAAAGTTATCTAGTAGTTCCATCAAAGACAAAACACTTGAGCCAATGGAGGTTTCATCATCAATCTCAAACTATAGAGACTCC
AGAGGAAGTGAGAAATTTACTGTATTGGCAACAACAGAGCAAGAGAACAAGTATCTGAATTTCATAGATGTATGGTCCGATGGAGTAAGATCATCTGAAA
AACAGAGTACAACTTCAACACCTGTTTCTTCTTCCAATGGCAATCTCTCTCTTTACTCGCTTGATCTCTCAATGGGAGGAAACAACTTAATGGGCCAAGA
CGAAATGGGCCTGATACAAATGGGCTTAGGTGTAATCGGGTCGGGTAGTGAGGATCATCACGGGTATGGTCCTTATGGTGTGACTTCTTCACTAGAGGAG
ATGTCAAGCTGGCTTGCTCCGATGTCTACCACACCTGGTGGACCATTAGCGGAGATACTGAGGCCGAGTACGAATTTGGCGATCTCTGGTGATATCGAAT
CGTATAGCTTGATGGAGACTCCCACTCCAAGCTCGTCCCCGTCTAGAGTGATGAAGAAGATGACTAGTTCAGTGTCCGACGAAAGCAGCCAGGTT

>IE_04F01_translated
MDFLKVSDKTTIPYRSDSLFSLNQQQYKESSFGFRDMEIHPHPTPYAGNGLLGCYYYYPFTNAQLKELERQAMIYKYMIASIPVPFDLLVSSPSSASPCN
NKNIAGDLEPGRCRRTDGKKWRCAKEVVSNHKYCEKHLHRGRPRSRKHVEPPYSRPNNNGGSVKNRDLKKLPQKLSSSSIKDKTLEPMEVSSSISNYRDS
RGSEKFTVLATTEQENKYLNFIDVWSDGVRSSEKQSTTSTPVSSSNGNLSLYSLDLSMGGNNLMGQDEMGLIQMGLGVIGSGSEDHHGYGPYGVTSSLEE
MSSWLAPMSTTPGGPLAEILRPSTNLAISGDIESYSLMETPTPSSSPSRVMKKMTSSVSDESSQV
Alignment ◆CDS（TAIRver.9）_without_stop_codonとのalignment◆

100%一致しました。

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