◆◆◆IE_04F03 detail◆◆◆

Code nameIE_04F03
LocusAT1G01350
TemplateIT_04F03 (HR3396)
Donor VectorpDONR207
Forward primerIPF_04F03 (ggggacaagtttgtacaaaaaagcaggcttcATGTCAGATTCTGGTGAGCCTAAACCTT)
Reverse primerIPR_04F03 (ggggaccactttgtacaagaaagctgggttCAATCCTTGTTCAGCTTTACTCCGTTCTTC)
Alignment with template seq.1stアミノ酸配列100%一致2nd-
Alignment with TAIR7CDSアミノ酸配列58.50%一致-
comment1st:Templateの配列とは一致
sequence 
>IE_04F03_1029bp
ATGTCAGATTCTGGTGAGCCTAAACCTTCTCAGCAAGAAGAACCTCTACCTCAACCTGCTGCCCAAGAAACTCAGTCTCAACAAGTTTGCACTTTCTTCA
AGAAGCCAACGAAAAGTAAAAACATAAGAAAAAGAACCATTGACGCTGATGAAGAAGACGGAGATTCGAAAAGCGAAAGCTCTATTCTACAAAATCTAAA
GAAAGTTGCAAAACCCGATAGCAAGTTATACTTTTCTTCTGGACCATCTAAGAGTTCTACTACAACGAGTGGAGCTCCTGAGAGGTCCGTCTTTCACTAC
GATTCATCCAAGGAAATCCAGGTTCAGAACGACAGTGGAGCAACAGCAACCCTTGAAACTGAGACCGACTTCAATCAAGACGCACGAGCTATCCGTGAGA
GAGTTCTTAAAAAAGCAGACGAAGCATTGAAAGGGAATAAGAAAAAGGCTTCTGATGAGAAGCTGTACAAAGGAATTCATGGATATACAGATCACAAAGC
TGGGTTTAGAAGAGAACAAACAATCTCGAGCGAGAAAGCTGGAGGCTCACACGGGCCATTAAGAGCTTCTGCTCACATAAGAGTGTCGGCTAGATTCGAT
TACCAGCCAGACATTTGCAAGGATTACAAAGAAACCGGATACTGTGGATATGGAGATTCGTGTAAGTTCTTGCATGACCGTGGGGATTACAAACCAGGAT
GGCAGATAGAGAAAGAGTGGGAAGAAGCAGAGAAAGTTAGGAAGAGAAATAAAGCTATGGGAGTTGAGGATGAAGATGATGAGGCTGACAAGGATAGCGA
CGAAGACGAAAATGCATTGCCCTTTGCTTGCTTCATTTGCAGGGAGCCTTTTGTTGATCCAGTTGTCACCAAATGCAAGCATTACTTCTGTGAGCATTGT
GCTTTAAAGCATCACACGAAGAACAAGAAGTGCTTTGTGTGTAACCAACCAACAATGGGGATTTTCAATGCAGCACATGAGATCAAGAAGAGAATGGCTG
AAGAACGGAGTAAAGCTGAACAAGGATTG

>IE_04F03_translated
MSDSGEPKPSQQEEPLPQPAAQETQSQQVCTFFKKPTKSKNIRKRTIDADEEDGDSKSESSILQNLKKVAKPDSKLYFSSGPSKSSTTTSGAPERSVFHY
DSSKEIQVQNDSGATATLETETDFNQDARAIRERVLKKADEALKGNKKKASDEKLYKGIHGYTDHKAGFRREQTISSEKAGGSHGPLRASAHIRVSARFD
YQPDICKDYKETGYCGYGDSCKFLHDRGDYKPGWQIEKEWEEAEKVRKRNKAMGVEDEDDEADKDSDEDENALPFACFICREPFVDPVVTKCKHYFCEHC
ALKHHTKNKKCFVCNQPTMGIFNAAHEIKKRMAEERSKAEQGL
Alignment ◆CDS（TAIRver.9）_without_stop_codonとのalignment◆

100%一致しました。

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