◆◆◆IE_04F04 detail◆◆◆

Code name	IE_04F04
Locus	AT5G24930
Template	IT_04F04 (HR3398)
Donor Vector	pDONR207
Forward primer	IPF_04F04 (ggggacaagtttgtacaaaaaagcaggcttcATGGCGTCAAAGCTCTGCGATTCGTGCA)
Reverse primer	IPR_04F04 (ggggaccactttgtacaagaaagctgggttAAATGTAGGTACAAGTCCGAACCCACTAAA)
Alignment with template seq.	1st	アミノ酸配列100%一致	2nd	-
Alignment with TAIR7CDS	1st	アミノ酸配列89.20%一致	2nd	-
comment	1st:Templateの配列とは一致
sequence	>IE_04F04_1086bp ATGGCGTCAAAGCTCTGCGATTCGTGCAAATCAGCGACGGCGGCGTTATACTGCCGACCAGACGCAGCGTTTCTATGCTTAAGCTGCGATTCAAAAGTCC ACGCGGCGAACAAACTCGCGTCACGACACGCGCGCGTGTGGATGTGTGAAGTGTGCGAGCAAGCACCGGCACACGTCACGTGCAAAGCAGACGCGGCCGC GTTATGCGTCACGTGCGACCGTGATATCCACTCGGCTAATCCGTTAGCTCGCCGTCACGAACGCGTTCCCGTTACTCCGTTTTACGATTCCGTCTCTAGT GACGGTTCCGTTAAACACACCGCCGTTAATTTCCTTGACGACTGTTACTTCTCCGATATAGATGGTAACGGAAGCCGTGAAGAAGAGGAGGAAGAAGCTG CTTCGTGGCTGTTACTTCCCAATCCAAAGACTACAACGACGGCGACGGCGGGGATCGTTGCTGTTACTTCAGCTGAGGAAGTTCCGGGAGATAGTCCGGA GATGAACACTGGTCAACAGTATTTGTTCTCGGATCCGGATCCGTATCTGGATCTGGATTATGGAAATGTTGATCCGAAGGTGGAATCGTTAGAGCAGAAT AGCTCCGGTACTGATGGTGTTGTTCCGGTGGAGAATCGGACGGTTAGGATTCCGACGGTGAACGAGAATTGCTTTGAAATGGATTTTACCGGAGGATCTA AAGGCTTTACTTATGGTGGTGGTTACAATTGCATCAGTCACAGCGTGTCATCATCGTCGATGGAAGTGGGAGTGGTGCCAGATGGTGGTTCGGTGGCTGA TGTATCGTATCCTTACGGTGGTCCTGCAACCAGTGGAGCTGATCCGGGGACACAGCGGGCTGTTCCGCTGACTTCAGCTGAGAGAGAAGCGAGGGTAATG AGGTACAGAGAGAAGAGGAAGAACAGGAAGTTTGAGAAGACTATAAGGTATGCTTCAAGGAAAGCGTATGCGGAAATGAGACCGAGAATCAAGGGACGGT TTGCGAAGCGGACGGATACAAATGAGAGCAATGATGTGGTTGGTCATGGTGGTATCTTTAGTGGGTTCGGACTTGTACCTACATTT >IE_04F04_translated MASKLCDSCKSATAALYCRPDAAFLCLSCDSKVHAANKLASRHARVWMCEVCEQAPAHVTCKADAAALCVTCDRDIHSANPLARRHERVPVTPFYDSVSS DGSVKHTAVNFLDDCYFSDIDGNGSREEEEEEAASWLLLPNPKTTTTATAGIVAVTSAEEVPGDSPEMNTGQQYLFSDPDPYLDLDYGNVDPKVESLEQN SSGTDGVVPVENRTVRIPTVNENCFEMDFTGGSKGFTYGGGYNCISHSVSSSSMEVGVVPDGGSVADVSYPYGGPATSGADPGTQRAVPLTSAEREARVM RYREKRKNRKFEKTIRYASRKAYAEMRPRIKGRFAKRTDTNESNDVVGHGGIFSGFGLVPTF
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 1086/1218 (89.2%)（アミノ酸配列で362/406 (89.2%)）一致しました。 10 20 30 40 50 60 70 80 90 100 AT5G24 ATGGACCCCACATGGATAGACTCATTGACACGCTCATGTGAAGCCAATAGTAACACCAACCACAAAAGAAAGAGAGAGAGAGAGACACTGAAACATAGAG IE_04F ---------------------------------------------------------------------------------------------------- 110 120 130 140 150 160 170 180 190 200 AT5G24 AGAAGAAAAAGAAGAGGTTTAGAGAGAGAAAAATGGCGTCAAAGCTCTGCGATTCGTGCAAATCAGCGACGGCGGCGTTATACTGCCGACCAGACGCAGC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F --------------------------------ATGGCGTCAAAGCTCTGCGATTCGTGCAAATCAGCGACGGCGGCGTTATACTGCCGACCAGACGCAGC 10 20 30 40 50 60 210 220 230 240 250 260 270 280 290 300 AT5G24 GTTTCTATGCTTAAGCTGCGATTCAAAAGTCCACGCGGCGAACAAACTCGCGTCACGACACGCGCGCGTGTGGATGTGTGAAGTGTGCGAGCAAGCACCG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F GTTTCTATGCTTAAGCTGCGATTCAAAAGTCCACGCGGCGAACAAACTCGCGTCACGACACGCGCGCGTGTGGATGTGTGAAGTGTGCGAGCAAGCACCG 70 80 90 100 110 120 130 140 150 160 310 320 330 340 350 360 370 380 390 400 AT5G24 GCACACGTCACGTGCAAAGCAGACGCGGCCGCGTTATGCGTCACGTGCGACCGTGATATCCACTCGGCTAATCCGTTAGCTCGCCGTCACGAACGCGTTC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F GCACACGTCACGTGCAAAGCAGACGCGGCCGCGTTATGCGTCACGTGCGACCGTGATATCCACTCGGCTAATCCGTTAGCTCGCCGTCACGAACGCGTTC 170 180 190 200 210 220 230 240 250 260 410 420 430 440 450 460 470 480 490 500 AT5G24 CCGTTACTCCGTTTTACGATTCCGTCTCTAGTGACGGTTCCGTTAAACACACCGCCGTTAATTTCCTTGACGACTGTTACTTCTCCGATATAGATGGTAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F CCGTTACTCCGTTTTACGATTCCGTCTCTAGTGACGGTTCCGTTAAACACACCGCCGTTAATTTCCTTGACGACTGTTACTTCTCCGATATAGATGGTAA 270 280 290 300 310 320 330 340 350 360 510 520 530 540 550 560 570 580 590 600 AT5G24 CGGAAGCCGTGAAGAAGAGGAGGAAGAAGCTGCTTCGTGGCTGTTACTTCCCAATCCAAAGACTACAACGACGGCGACGGCGGGGATCGTTGCTGTTACT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F CGGAAGCCGTGAAGAAGAGGAGGAAGAAGCTGCTTCGTGGCTGTTACTTCCCAATCCAAAGACTACAACGACGGCGACGGCGGGGATCGTTGCTGTTACT 370 380 390 400 410 420 430 440 450 460 610 620 630 640 650 660 670 680 690 700 AT5G24 TCAGCTGAGGAAGTTCCGGGAGATAGTCCGGAGATGAACACTGGTCAACAGTATTTGTTCTCGGATCCGGATCCGTATCTGGATCTGGATTATGGAAATG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F TCAGCTGAGGAAGTTCCGGGAGATAGTCCGGAGATGAACACTGGTCAACAGTATTTGTTCTCGGATCCGGATCCGTATCTGGATCTGGATTATGGAAATG 470 480 490 500 510 520 530 540 550 560 710 720 730 740 750 760 770 780 790 800 AT5G24 TTGATCCGAAGGTGGAATCGTTAGAGCAGAATAGCTCCGGTACTGATGGTGTTGTTCCGGTGGAGAATCGGACGGTTAGGATTCCGACGGTGAACGAGAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F TTGATCCGAAGGTGGAATCGTTAGAGCAGAATAGCTCCGGTACTGATGGTGTTGTTCCGGTGGAGAATCGGACGGTTAGGATTCCGACGGTGAACGAGAA 570 580 590 600 610 620 630 640 650 660 810 820 830 840 850 860 870 880 890 900 AT5G24 TTGCTTTGAAATGGATTTTACCGGAGGATCTAAAGGCTTTACTTATGGTGGTGGTTACAATTGCATCAGTCACAGCGTGTCATCATCGTCGATGGAAGTG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F TTGCTTTGAAATGGATTTTACCGGAGGATCTAAAGGCTTTACTTATGGTGGTGGTTACAATTGCATCAGTCACAGCGTGTCATCATCGTCGATGGAAGTG 670 680 690 700 710 720 730 740 750 760 910 920 930 940 950 960 970 980 990 1000 AT5G24 GGAGTGGTGCCAGATGGTGGTTCGGTGGCTGATGTATCGTATCCTTACGGTGGTCCTGCAACCAGTGGAGCTGATCCGGGGACACAGCGGGCTGTTCCGC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F GGAGTGGTGCCAGATGGTGGTTCGGTGGCTGATGTATCGTATCCTTACGGTGGTCCTGCAACCAGTGGAGCTGATCCGGGGACACAGCGGGCTGTTCCGC 770 780 790 800 810 820 830 840 850 860 1010 1020 1030 1040 1050 1060 1070 1080 1090 1100 AT5G24 TGACTTCAGCTGAGAGAGAAGCGAGGGTAATGAGGTACAGAGAGAAGAGGAAGAACAGGAAGTTTGAGAAGACTATAAGGTATGCTTCAAGGAAAGCGTA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F TGACTTCAGCTGAGAGAGAAGCGAGGGTAATGAGGTACAGAGAGAAGAGGAAGAACAGGAAGTTTGAGAAGACTATAAGGTATGCTTCAAGGAAAGCGTA 870 880 890 900 910 920 930 940 950 960 1110 1120 1130 1140 1150 1160 1170 1180 1190 1200 AT5G24 TGCGGAAATGAGACCGAGAATCAAGGGACGGTTTGCGAAGCGGACGGATACAAATGAGAGCAATGATGTGGTTGGTCATGGTGGTATCTTTAGTGGGTTC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F TGCGGAAATGAGACCGAGAATCAAGGGACGGTTTGCGAAGCGGACGGATACAAATGAGAGCAATGATGTGGTTGGTCATGGTGGTATCTTTAGTGGGTTC 970 980 990 1000 1010 1020 1030 1040 1050 1060 1210 AT5G24 GGACTTGTACCTACATTT :::::::::::::::::: IE_04F GGACTTGTACCTACATTT 1070 1080 10 20 30 40 50 60 70 80 90 100 AT5G24 MDPTWIDSLTRSCEANSNTNHKRKRERETLKHREKKKKRFRERKMASKLCDSCKSATAALYCRPDAAFLCLSCDSKVHAANKLASRHARVWMCEVCEQAP :::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F --------------------------------------------MASKLCDSCKSATAALYCRPDAAFLCLSCDSKVHAANKLASRHARVWMCEVCEQAP 10 20 30 40 50 110 120 130 140 150 160 170 180 190 200 AT5G24 AHVTCKADAAALCVTCDRDIHSANPLARRHERVPVTPFYDSVSSDGSVKHTAVNFLDDCYFSDIDGNGSREEEEEEAASWLLLPNPKTTTTATAGIVAVT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F AHVTCKADAAALCVTCDRDIHSANPLARRHERVPVTPFYDSVSSDGSVKHTAVNFLDDCYFSDIDGNGSREEEEEEAASWLLLPNPKTTTTATAGIVAVT 60 70 80 90 100 110 120 130 140 150 210 220 230 240 250 260 270 280 290 300 AT5G24 SAEEVPGDSPEMNTGQQYLFSDPDPYLDLDYGNVDPKVESLEQNSSGTDGVVPVENRTVRIPTVNENCFEMDFTGGSKGFTYGGGYNCISHSVSSSSMEV :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F SAEEVPGDSPEMNTGQQYLFSDPDPYLDLDYGNVDPKVESLEQNSSGTDGVVPVENRTVRIPTVNENCFEMDFTGGSKGFTYGGGYNCISHSVSSSSMEV 160 170 180 190 200 210 220 230 240 250 310 320 330 340 350 360 370 380 390 400 AT5G24 GVVPDGGSVADVSYPYGGPATSGADPGTQRAVPLTSAEREARVMRYREKRKNRKFEKTIRYASRKAYAEMRPRIKGRFAKRTDTNESNDVVGHGGIFSGF :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F GVVPDGGSVADVSYPYGGPATSGADPGTQRAVPLTSAEREARVMRYREKRKNRKFEKTIRYASRKAYAEMRPRIKGRFAKRTDTNESNDVVGHGGIFSGF 260 270 280 290 300 310 320 330 340 350 AT5G24 GLVPTF :::::: IE_04F GLVPTF 360

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