◆◆◆IE_04F05 detail◆◆◆

Code name	IE_04F05
Locus	AT3G24140
Template	IT_04F05 (HR3406)
Donor Vector	pDONR207
Forward primer	IPF_04F05 (ggggacaagtttgtacaaaaaagcaggcttcATGACTCATATCGCGGTCGAAAGAAACC)
Reverse primer	IPR_04F05 (ggggaccactttgtacaagaaagctgggttAGTAAACACAATATTTCCCAGGTTAGAGCT)
Alignment with template seq.	1st	アミノ酸配列100%一致	2nd	-
Alignment with TAIR7CDS	1st	アミノ酸配列52.70%一致	2nd	-
comment	1st:Templateの配列とは一致
sequence	>IE_04F05_654bp ATGACTCATATCGCGGTCGAAAGAAACCGTAGGAAGCAAATGAATGAGCATCTTCGTGTCCTTAGATCTCTCATGCCTGGCTCCTACGTTCAAAGGGGAG ACCAAGCGTCAATCATAGGAGGAGCAATAGAGTTTGTGAGAGAGCTCGAGCAACTCCTACAATGTCTTGAATCACAGAAGCGTCGAAGAATCTTAGGAGA AACCGGTAGGGACATGACAACGACAACGACTTCTTCTTCTTCTCCCATAACTACGGTAGCGAACCAAGCACAACCGCTCATTATTACGGGAAATGTAACC GAGCTAGAGGGCGGAGGAGGGCTTCGGGAGGAGACTGCGGAGAACAAGTCGTGCTTGGCTGACGTGGAGGTGAAGCTGCTAGGGTTTGACGCCATGATCA AGATACTTTCAAGAAGAAGGCCGGGACAGCTGATTAAGACTATAGCTGCTTTGGAGGATCTTCATCTCTCTATTCTTCACACTAACATCACTACCATGGA ACAAACCGTCCTCTACTCCTTTAATGTCAAGATAACAAGTGAAACGAGGTTTACGGCAGAAGACATAGCAAGTTCCATCCAACAGATATTTAGTTTCATT CATGCAAATACCAACATATCTGGAAGCTCTAACCTGGGAAATATTGTGTTTACT >IE_04F05_translated MTHIAVERNRRKQMNEHLRVLRSLMPGSYVQRGDQASIIGGAIEFVRELEQLLQCLESQKRRRILGETGRDMTTTTTSSSSPITTVANQAQPLIITGNVT ELEGGGGLREETAENKSCLADVEVKLLGFDAMIKILSRRRPGQLIKTIAALEDLHLSILHTNITTMEQTVLYSFNVKITSETRFTAEDIASSIQQIFSFI HANTNISGSSNLGNIVFT
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 654/1242 (52.7%)（アミノ酸配列で218/414 (52.7%)）一致しました。 10 20 30 40 50 60 70 80 90 100 AT3G24 ATGGATAAAGATTACTCGGCACCAAACTTCTTAGGTGAATCCTCAGGCGGTAACGATGATAACAGCTCTGGTATGATAGACTATATGTTCAATAGAAACC IE_04F ---------------------------------------------------------------------------------------------------- 110 120 130 140 150 160 170 180 190 200 AT3G24 TTCAACAACAACAAAAGCAATCGATGCCACAACAGCAGCAACATCAACTCTCTCCTTCCGGATTTGGAGCAACACCTTTTGATAAAATGAACTTCTCTGA IE_04F ---------------------------------------------------------------------------------------------------- 210 220 230 240 250 260 270 280 290 300 AT3G24 TGTGATGCAGTTTGCGGACTTCGGTTCGAAACTTGCGTTGAACCAGACCAGAAACCAAGACGATCAAGAAACCGGGATTGACCCCGTTTATTTCTTGAAG IE_04F ---------------------------------------------------------------------------------------------------- 310 320 330 340 350 360 370 380 390 400 AT3G24 TTCCCTGTCTTGAACGACAAAATAGAGGACCATAACCAAACCCAACATCTCATGCCTTCTCATCAGACGTCTCAAGAAGGAGGTGAGTGTGGAGGAAACA IE_04F ---------------------------------------------------------------------------------------------------- 410 420 430 440 450 460 470 480 490 500 AT3G24 TAGGCAATGTGTTTCTTGAAGAAAAAGAAGATCAAGACGATGACAACGACAACAACTCCGTGCAACTACGTTTTATTGGAGGAGAAGAAGAAGATAGGGA IE_04F ---------------------------------------------------------------------------------------------------- 510 520 530 540 550 560 570 580 590 600 AT3G24 GAACAAGAATGTTACGAAAAAGGAGGTGAAGAGCAAGAGGAAGAGAGCTAGAACGAGCAAGACCAGCGAAGAAGTGGAAAGCCAACGGATGACTCATATC :::::::::::: IE_04F ----------------------------------------------------------------------------------------ATGACTCATATC 10 610 620 630 640 650 660 670 680 690 700 AT3G24 GCGGTCGAAAGAAACCGTAGGAAGCAAATGAATGAGCATCTTCGTGTCCTTAGATCTCTCATGCCTGGCTCCTACGTTCAAAGGGGAGACCAAGCGTCAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F GCGGTCGAAAGAAACCGTAGGAAGCAAATGAATGAGCATCTTCGTGTCCTTAGATCTCTCATGCCTGGCTCCTACGTTCAAAGGGGAGACCAAGCGTCAA 20 30 40 50 60 70 80 90 100 110 710 720 730 740 750 760 770 780 790 800 AT3G24 TCATAGGAGGAGCAATAGAGTTTGTGAGAGAGCTCGAGCAACTCCTACAATGTCTTGAATCACAGAAGCGTCGAAGAATCTTAGGAGAAACCGGTAGGGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F TCATAGGAGGAGCAATAGAGTTTGTGAGAGAGCTCGAGCAACTCCTACAATGTCTTGAATCACAGAAGCGTCGAAGAATCTTAGGAGAAACCGGTAGGGA 120 130 140 150 160 170 180 190 200 210 810 820 830 840 850 860 870 880 890 900 AT3G24 CATGACAACGACAACGACTTCTTCTTCTTCTCCCATAACTACGGTAGCGAACCAAGCACAACCGCTCATTATTACGGGAAATGTAACCGAGCTAGAGGGC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F CATGACAACGACAACGACTTCTTCTTCTTCTCCCATAACTACGGTAGCGAACCAAGCACAACCGCTCATTATTACGGGAAATGTAACCGAGCTAGAGGGC 220 230 240 250 260 270 280 290 300 310 910 920 930 940 950 960 970 980 990 1000 AT3G24 GGAGGAGGGCTTCGGGAGGAGACTGCGGAGAACAAGTCGTGCTTGGCTGACGTGGAGGTGAAGCTGCTAGGGTTTGACGCCATGATCAAGATACTTTCAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F GGAGGAGGGCTTCGGGAGGAGACTGCGGAGAACAAGTCGTGCTTGGCTGACGTGGAGGTGAAGCTGCTAGGGTTTGACGCCATGATCAAGATACTTTCAA 320 330 340 350 360 370 380 390 400 410 1010 1020 1030 1040 1050 1060 1070 1080 1090 1100 AT3G24 GAAGAAGGCCGGGACAGCTGATTAAGACTATAGCTGCTTTGGAGGATCTTCATCTCTCTATTCTTCACACTAACATCACTACCATGGAACAAACCGTCCT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F GAAGAAGGCCGGGACAGCTGATTAAGACTATAGCTGCTTTGGAGGATCTTCATCTCTCTATTCTTCACACTAACATCACTACCATGGAACAAACCGTCCT 420 430 440 450 460 470 480 490 500 510 1110 1120 1130 1140 1150 1160 1170 1180 1190 1200 AT3G24 CTACTCCTTTAATGTCAAGATAACAAGTGAAACGAGGTTTACGGCAGAAGACATAGCAAGTTCCATCCAACAGATATTTAGTTTCATTCATGCAAATACC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F CTACTCCTTTAATGTCAAGATAACAAGTGAAACGAGGTTTACGGCAGAAGACATAGCAAGTTCCATCCAACAGATATTTAGTTTCATTCATGCAAATACC 520 530 540 550 560 570 580 590 600 610 1210 1220 1230 1240 AT3G24 AACATATCTGGAAGCTCTAACCTGGGAAATATTGTGTTTACT :::::::::::::::::::::::::::::::::::::::::: IE_04F AACATATCTGGAAGCTCTAACCTGGGAAATATTGTGTTTACT 620 630 640 650 10 20 30 40 50 60 70 80 90 100 AT3G24 MDKDYSAPNFLGESSGGNDDNSSGMIDYMFNRNLQQQQKQSMPQQQQHQLSPSGFGATPFDKMNFSDVMQFADFGSKLALNQTRNQDDQETGIDPVYFLK IE_04F ---------------------------------------------------------------------------------------------------- 110 120 130 140 150 160 170 180 190 200 AT3G24 FPVLNDKIEDHNQTQHLMPSHQTSQEGGECGGNIGNVFLEEKEDQDDDNDNNSVQLRFIGGEEEDRENKNVTKKEVKSKRKRARTSKTSEEVESQRMTHI :::: IE_04F ------------------------------------------------------------------------------------------------MTHI 210 220 230 240 250 260 270 280 290 300 AT3G24 AVERNRRKQMNEHLRVLRSLMPGSYVQRGDQASIIGGAIEFVRELEQLLQCLESQKRRRILGETGRDMTTTTTSSSSPITTVANQAQPLIITGNVTELEG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F AVERNRRKQMNEHLRVLRSLMPGSYVQRGDQASIIGGAIEFVRELEQLLQCLESQKRRRILGETGRDMTTTTTSSSSPITTVANQAQPLIITGNVTELEG 10 20 30 40 50 60 70 80 90 100 310 320 330 340 350 360 370 380 390 400 AT3G24 GGGLREETAENKSCLADVEVKLLGFDAMIKILSRRRPGQLIKTIAALEDLHLSILHTNITTMEQTVLYSFNVKITSETRFTAEDIASSIQQIFSFIHANT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F GGGLREETAENKSCLADVEVKLLGFDAMIKILSRRRPGQLIKTIAALEDLHLSILHTNITTMEQTVLYSFNVKITSETRFTAEDIASSIQQIFSFIHANT 110 120 130 140 150 160 170 180 190 200 410 AT3G24 NISGSSNLGNIVFT :::::::::::::: IE_04F NISGSSNLGNIVFT 210

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