◆◆◆IE_04F06 detail◆◆◆

Code name	IE_04F06
Locus	AT3G62090
Template	IT_04F06 (HR3408)
Donor Vector	pDONR207
Forward primer	IPF_04F06 (ggggacaagtttgtacaaaaaagcaggcttcATGACTTCCCCATCATCCACCTTCAGAC)
Reverse primer	IPR_04F06 (ggggaccactttgtacaagaaagctgggttTCTGTTAGTTTTCCTTGATTTCTTGTGTAA)
Alignment with template seq.	1st	アミノ酸配列100%一致	2nd	-
Alignment with TAIR7CDS	1st	アミノ酸配列95.30%一致	2nd	-
comment	1st:Templateの配列とは一致
sequence	>IE_04F06_1089bp ATGACTTCCCCATCATCCACCTTCAGACCAAAGTTAAGCGATCAAGAGTATATGGAGCTTGTGTTTGAGAATGGCCAGATTCTTGCAAAGGGCCAAAGAT CCAACGTTTCTCTGCATAATCAACGTACCAAATCGATCATGGATTTGTATGAGGCAGAGTATAACGAGGATTTCATGAAGAGTATCATCCATGGTGGTGG TGGTGCCATCACAAATCTCGGGGACACGCAGGTTGTTCCACAAAGTCATGTTGCTGCTGCCCATGAAACAAACATGTTGGAAAGCAATAAACATGTTGAC GATTCTGAGACTTTGAAAGCTTCTTCATCAAAGAGGATGATGGTTGATTATCATAACCGAAAGAAGATCAAGTTTATACCTCCTGATGAGCAATCCGTGG TTGCTGATAGGTCGTTCAAATTGGGCTTTGACACTTCCTCCGTAGGTTTCACTGAAGACAGTGAAGGATCGATGTATCTAAGCAGTAGTCTAGATGACGA GTCAGATGATGCGAGGCCACAAGTTCCTGCAAGAACAAGAAAAGCTTTGGTCAAAAGAAAACGAAATGCAGAAGCGTATAATTCACCTGAGAGAAATCAA AGAAATGATATCAACAAGAAAATGCGTACTTTGCAAAATCTACTACCTAACTCTCACAAGGACGACAACGAATCGATGTTGGATGAAGCAATCAATTATA TGACAAACCTTCAACTTCAAGTTCAGATGATGACGATGGGTAACAGATTTGTTACACCATCAATGATGATGCCTTTGGGGCCGAACTACTCTCAGATGGG TCTAGCAATGGGTGTGGGAATGCAAATGGGCGAACAACAGTTTCTGCCTGCACATGTTCTAGGAGCTGGCTTGCCTGGGATTAATGATTCAGCAGATATG CTAAGGTTTCTTAACCATCCTGGACTAATGCCAATGCAAAACTCTGCACCTTTCATTCCAACGGAAAATTGTTCCCCACAATCTGTCCCTCCATCGTGCG CTGCTTTCCCTAACCAAATACCAAATCCCAACTCTTTGTCAAATTTAGATGGTGCAACCTTACACAAGAAATCAAGGAAAACTAACAGA >IE_04F06_translated MTSPSSTFRPKLSDQEYMELVFENGQILAKGQRSNVSLHNQRTKSIMDLYEAEYNEDFMKSIIHGGGGAITNLGDTQVVPQSHVAAAHETNMLESNKHVD DSETLKASSSKRMMVDYHNRKKIKFIPPDEQSVVADRSFKLGFDTSSVGFTEDSEGSMYLSSSLDDESDDARPQVPARTRKALVKRKRNAEAYNSPERNQ RNDINKKMRTLQNLLPNSHKDDNESMLDEAINYMTNLQLQVQMMTMGNRFVTPSMMMPLGPNYSQMGLAMGVGMQMGEQQFLPAHVLGAGLPGINDSADM LRFLNHPGLMPMQNSAPFIPTENCSPQSVPPSCAAFPNQIPNPNSLSNLDGATLHKKSRKTNR
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 1038/1089 (95.3%)（アミノ酸配列で346/363 (95.3%)）一致しました。 10 20 30 40 AT3G62 ---------------------------------------------------ATGGAGCTTGTGTTTGAGAATGGCCAGATTCTTGCAAAGGGCCAAAGAT ::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F ATGACTTCCCCATCATCCACCTTCAGACCAAAGTTAAGCGATCAAGAGTATATGGAGCTTGTGTTTGAGAATGGCCAGATTCTTGCAAAGGGCCAAAGAT 10 20 30 40 50 60 70 80 90 100 50 60 70 80 90 100 110 120 130 140 AT3G62 CCAACGTTTCTCTGCATAATCAACGTACCAAATCGATCATGGATTTGTATGAGGCAGAGTATAACGAGGATTTCATGAAGAGTATCATCCATGGTGGTGG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F CCAACGTTTCTCTGCATAATCAACGTACCAAATCGATCATGGATTTGTATGAGGCAGAGTATAACGAGGATTTCATGAAGAGTATCATCCATGGTGGTGG 110 120 130 140 150 160 170 180 190 200 150 160 170 180 190 200 210 220 230 240 AT3G62 TGGTGCCATCACAAATCTCGGGGACACGCAGGTTGTTCCACAAAGTCATGTTGCTGCTGCCCATGAAACAAACATGTTGGAAAGCAATAAACATGTTGAC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F TGGTGCCATCACAAATCTCGGGGACACGCAGGTTGTTCCACAAAGTCATGTTGCTGCTGCCCATGAAACAAACATGTTGGAAAGCAATAAACATGTTGAC 210 220 230 240 250 260 270 280 290 300 250 260 270 280 290 300 310 320 330 340 AT3G62 GATTCTGAGACTTTGAAAGCTTCTTCATCAAAGAGGATGATGGTTGATTATCATAACCGAAAGAAGATCAAGTTTATACCTCCTGATGAGCAATCCGTGG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F GATTCTGAGACTTTGAAAGCTTCTTCATCAAAGAGGATGATGGTTGATTATCATAACCGAAAGAAGATCAAGTTTATACCTCCTGATGAGCAATCCGTGG 310 320 330 340 350 360 370 380 390 400 350 360 370 380 390 400 410 420 430 440 AT3G62 TTGCTGATAGGTCGTTCAAATTGGGCTTTGACACTTCCTCCGTAGGTTTCACTGAAGACAGTGAAGGATCGATGTATCTAAGCAGTAGTCTAGATGACGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F TTGCTGATAGGTCGTTCAAATTGGGCTTTGACACTTCCTCCGTAGGTTTCACTGAAGACAGTGAAGGATCGATGTATCTAAGCAGTAGTCTAGATGACGA 410 420 430 440 450 460 470 480 490 500 450 460 470 480 490 500 510 520 530 540 AT3G62 GTCAGATGATGCGAGGCCACAAGTTCCTGCAAGAACAAGAAAAGCTTTGGTCAAAAGAAAACGAAATGCAGAAGCGTATAATTCACCTGAGAGAAATCAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F GTCAGATGATGCGAGGCCACAAGTTCCTGCAAGAACAAGAAAAGCTTTGGTCAAAAGAAAACGAAATGCAGAAGCGTATAATTCACCTGAGAGAAATCAA 510 520 530 540 550 560 570 580 590 600 550 560 570 580 590 600 610 620 630 640 AT3G62 AGAAATGATATCAACAAGAAAATGCGTACTTTGCAAAATCTACTACCTAACTCTCACAAGGACGACAACGAATCGATGTTGGATGAAGCAATCAATTATA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F AGAAATGATATCAACAAGAAAATGCGTACTTTGCAAAATCTACTACCTAACTCTCACAAGGACGACAACGAATCGATGTTGGATGAAGCAATCAATTATA 610 620 630 640 650 660 670 680 690 700 650 660 670 680 690 700 710 720 730 740 AT3G62 TGACAAACCTTCAACTTCAAGTTCAGATGATGACGATGGGTAACAGATTTGTTACACCATCAATGATGATGCCTTTGGGGCCGAACTACTCTCAGATGGG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F TGACAAACCTTCAACTTCAAGTTCAGATGATGACGATGGGTAACAGATTTGTTACACCATCAATGATGATGCCTTTGGGGCCGAACTACTCTCAGATGGG 710 720 730 740 750 760 770 780 790 800 750 760 770 780 790 800 810 820 830 840 AT3G62 TCTAGCAATGGGTGTGGGAATGCAAATGGGCGAACAACAGTTTCTGCCTGCACATGTTCTAGGAGCTGGCTTGCCTGGGATTAATGATTCAGCAGATATG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F TCTAGCAATGGGTGTGGGAATGCAAATGGGCGAACAACAGTTTCTGCCTGCACATGTTCTAGGAGCTGGCTTGCCTGGGATTAATGATTCAGCAGATATG 810 820 830 840 850 860 870 880 890 900 850 860 870 880 890 900 910 920 930 940 AT3G62 CTAAGGTTTCTTAACCATCCTGGACTAATGCCAATGCAAAACTCTGCACCTTTCATTCCAACGGAAAATTGTTCCCCACAATCTGTCCCTCCATCGTGCG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F CTAAGGTTTCTTAACCATCCTGGACTAATGCCAATGCAAAACTCTGCACCTTTCATTCCAACGGAAAATTGTTCCCCACAATCTGTCCCTCCATCGTGCG 910 920 930 940 950 960 970 980 990 1000 950 960 970 980 990 1000 1010 1020 1030 AT3G62 CTGCTTTCCCTAACCAAATACCAAATCCCAACTCTTTGTCAAATTTAGATGGTGCAACCTTACACAAGAAATCAAGGAAAACTAACAGA ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F CTGCTTTCCCTAACCAAATACCAAATCCCAACTCTTTGTCAAATTTAGATGGTGCAACCTTACACAAGAAATCAAGGAAAACTAACAGA 1010 1020 1030 1040 1050 1060 1070 1080 10 20 30 40 50 60 70 80 AT3G62 -----------------MELVFENGQILAKGQRSNVSLHNQRTKSIMDLYEAEYNEDFMKSIIHGGGGAITNLGDTQVVPQSHVAAAHETNMLESNKHVD ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F MTSPSSTFRPKLSDQEYMELVFENGQILAKGQRSNVSLHNQRTKSIMDLYEAEYNEDFMKSIIHGGGGAITNLGDTQVVPQSHVAAAHETNMLESNKHVD 10 20 30 40 50 60 70 80 90 100 90 100 110 120 130 140 150 160 170 180 AT3G62 DSETLKASSSKRMMVDYHNRKKIKFIPPDEQSVVADRSFKLGFDTSSVGFTEDSEGSMYLSSSLDDESDDARPQVPARTRKALVKRKRNAEAYNSPERNQ :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F DSETLKASSSKRMMVDYHNRKKIKFIPPDEQSVVADRSFKLGFDTSSVGFTEDSEGSMYLSSSLDDESDDARPQVPARTRKALVKRKRNAEAYNSPERNQ 110 120 130 140 150 160 170 180 190 200 190 200 210 220 230 240 250 260 270 280 AT3G62 RNDINKKMRTLQNLLPNSHKDDNESMLDEAINYMTNLQLQVQMMTMGNRFVTPSMMMPLGPNYSQMGLAMGVGMQMGEQQFLPAHVLGAGLPGINDSADM :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F RNDINKKMRTLQNLLPNSHKDDNESMLDEAINYMTNLQLQVQMMTMGNRFVTPSMMMPLGPNYSQMGLAMGVGMQMGEQQFLPAHVLGAGLPGINDSADM 210 220 230 240 250 260 270 280 290 300 290 300 310 320 330 340 AT3G62 LRFLNHPGLMPMQNSAPFIPTENCSPQSVPPSCAAFPNQIPNPNSLSNLDGATLHKKSRKTNR ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F LRFLNHPGLMPMQNSAPFIPTENCSPQSVPPSCAAFPNQIPNPNSLSNLDGATLHKKSRKTNR 310 320 330 340 350 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