◆◆◆IE_04F07 detail◆◆◆

Code name	IE_04F07
Locus	AT3G54990
Template	IT_04F07 (HR3419)
Donor Vector	pDONR207
Forward primer	IPF_04F07 (ggggacaagtttgtacaaaaaagcaggcttcATGTTGGATCTTAACCTAAAGATCTTTT)
Reverse primer	IPR_04F07 (ggggaccactttgtacaagaaagctgggttAAAGATTACGTGTCTAACAAAATGGTACCT)
Alignment with template seq.	1st	アミノ酸配列100%一致	2nd	-
Alignment with TAIR7CDS	1st	アミノ酸配列68.80%一致	2nd	-
comment	1st:Templateの配列とは一致
sequence	>IE_04F07_741bp ATGTTGGATCTTAACCTAAAGATCTTTTCTTCTTATAACGAAGATCAAGATCGGAAAGTACCATTAATGATCTCAACCACCGGTGAAGAAGAATCTAACT CATCTTCCTCCTCCACAACAGACTCTGCAGCGAGAGATGCTTTCATCGCTTTTGGAATTCTCAAACGCGACGATGACCTTGTTCCTCCTCCTCCTCCTCC TCCTCATAAAGAAACAGGAGATCTCTTTCCGGTGGTGGCTGATGCTCGTCGGAATATAGAATTCTCCGTGGAAGACAGTCACTGGTTGAATCTTTCTTCT TTACAAAGAAATACACAGAAAATGGTGAAGAAGAGCAGAAGAGGACCAAGGTCTCGTAGCTCCCAATATCGTGGCGTCACTTTTTACCGTCGCACCGGTC GTTGGGAATCTCATATTTGGGATTGTGGAAAGCAAGTTTATTTGGGCGGGTTTGATACTGCTTACGCAGCAGCAAGGGCTTACGACCGAGCTGCTATCAA ATTCCGTGGTCTCGATGCAGACATCAATTTCGTCGTGGATGATTATAGGCATGACATCGATAAGATGAAGAATTTAAATAAGGTGGAGTTCGTGCAAACA CTTAGGCGAGAGAGTGCGAGTTTCGGAAGAGGAAGTTCCAAATACAAAGGCTTGGCTCTTCAAAAATGCACCCAATTCAAAACTCATGATCAGATTCATC TCTTCCAAAACAGGTACCATTTTGTTAGACACGTAATCTTT >IE_04F07_translated MLDLNLKIFSSYNEDQDRKVPLMISTTGEEESNSSSSSTTDSAARDAFIAFGILKRDDDLVPPPPPPPHKETGDLFPVVADARRNIEFSVEDSHWLNLSS LQRNTQKMVKKSRRGPRSRSSQYRGVTFYRRTGRWESHIWDCGKQVYLGGFDTAYAAARAYDRAAIKFRGLDADINFVVDDYRHDIDKMKNLNKVEFVQT LRRESASFGRGSSKYKGLALQKCTQFKTHDQIHLFQNRYHFVRHVIF
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 735/1039 (70.7%)（アミノ酸配列で238/346 (68.8%)）一致しました。 10 20 30 40 50 60 70 80 90 100 AT3G54 ATGTTGGATCTTAACCTAAAGATCTTTTCTTCTTATAACGAAGATCAAGATCGGAAAGTACCATTAATGATCTCAACCACCGGTGAAGAAGAATCTAACT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F ATGTTGGATCTTAACCTAAAGATCTTTTCTTCTTATAACGAAGATCAAGATCGGAAAGTACCATTAATGATCTCAACCACCGGTGAAGAAGAATCTAACT 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT3G54 CATCTTCCTCCTCCACAACAGACTCTGCAGCGAGAGATGCTTTCATCGCTTTTGGAATTCTCAAACGCGACGATGACCTTGTTCCTCCTCCTCCTCCTCC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F CATCTTCCTCCTCCACAACAGACTCTGCAGCGAGAGATGCTTTCATCGCTTTTGGAATTCTCAAACGCGACGATGACCTTGTTCCTCCTCCTCCTCCTCC 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT3G54 TCCTCATAAAGAAACAGGAGATCTCTTTCCGGTGGTGGCTGATGCTCGTCGGAATATAGAATTCTCCGTGGAAGACAGTCACTGGTTGAATCTTTCTTCT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F TCCTCATAAAGAAACAGGAGATCTCTTTCCGGTGGTGGCTGATGCTCGTCGGAATATAGAATTCTCCGTGGAAGACAGTCACTGGTTGAATCTTTCTTCT 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 AT3G54 TTACAAAGAAATACACAGAAAATGGTGAAGAAGAGCAGAAGAGGACCAAGGTCTCGTAGCTCCCAATATCGTGGCGTCACTTTTTACCGTCGCACCGGTC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F TTACAAAGAAATACACAGAAAATGGTGAAGAAGAGCAGAAGAGGACCAAGGTCTCGTAGCTCCCAATATCGTGGCGTCACTTTTTACCGTCGCACCGGTC 310 320 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 480 490 500 AT3G54 GTTGGGAATCTCATATTTGGGATTGTGGAAAGCAAGTTTATTTGGGCGGGTTTGATACTGCTTACGCAGCAGCAAGGGCTTACGACCGAGCTGCTATCAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F GTTGGGAATCTCATATTTGGGATTGTGGAAAGCAAGTTTATTTGGGCGGGTTTGATACTGCTTACGCAGCAGCAAGGGCTTACGACCGAGCTGCTATCAA 410 420 430 440 450 460 470 480 490 500 510 520 530 540 550 560 570 580 590 600 AT3G54 ATTCCGTGGTCTCGATGCAGACATCAATTTCGTCGTGGATGATTATAGGCATGACATCGATAAGATGAAGAATTTAAATAAGGTGGAGTTCGTGCAAACA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F ATTCCGTGGTCTCGATGCAGACATCAATTTCGTCGTGGATGATTATAGGCATGACATCGATAAGATGAAGAATTTAAATAAGGTGGAGTTCGTGCAAACA 510 520 530 540 550 560 570 580 590 600 610 620 630 640 650 660 670 680 690 700 AT3G54 CTTAGGCGAGAGAGTGCGAGTTTCGGAAGAGGAAGTTCCAAATACAAAGGCTTGGCTCTTCAAAAATGCACCCAATTCAAAACTCATGATCAGATTCATC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F CTTAGGCGAGAGAGTGCGAGTTTCGGAAGAGGAAGTTCCAAATACAAAGGCTTGGCTCTTCAAAAATGCACCCAATTCAAAACTCATGATCAGATTCATC 610 620 630 640 650 660 670 680 690 700 710 720 730 740 750 760 770 780 790 800 AT3G54 TCTTCCAAAACAGGGGATGGGATGCAGCAGCAATAAAATACAATGAGTTGGGAAAGGGAGAAGGAGCCATGAAGTTTGGTGCCCATATCAAAGGAAATGG :::::::::::::: ::: :: :::: : :: IE_04F TCTTCCAAAACAGG------------------------TACCAT------------------------------TTTGTT-----------AG------- 710 720 810 820 830 840 850 860 870 880 890 AT3G54 TCACA-ATGATCTTGAACTAAGTCTCGGAATTTCATCATCATCGGAAAGTATAAAGTTGACAACAGGCGATTACTATAAGGGTATCAATCGGTCCACGAT ::: : ::::: IE_04F --ACACGTAATCTTT------------------------------------------------------------------------------------- 730 740 900 910 920 930 940 950 960 970 980 990 AT3G54 GGGTTTATACGGTAAGCAATCATCGATATTTTTACCCATGGCAACCATGAAACCTCTGAAGACAGTTGCAGCATCATCAGGATTCCCTTTTATCAGCATG IE_04F ---------------------------------------------------------------------------------------------------- 1000 1010 1020 1030 AT3G54 ACAAGTTCCTCTTCCTCCATGTCCAATTGTTTTGATCCA IE_04F --------------------------------------- 10 20 30 40 50 60 70 80 90 100 AT3G54 MLDLNLKIFSSYNEDQDRKVPLMISTTGEEESNSSSSSTTDSAARDAFIAFGILKRDDDLVPPPPPPPHKETGDLFPVVADARRNIEFSVEDSHWLNLSS :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F MLDLNLKIFSSYNEDQDRKVPLMISTTGEEESNSSSSSTTDSAARDAFIAFGILKRDDDLVPPPPPPPHKETGDLFPVVADARRNIEFSVEDSHWLNLSS 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT3G54 LQRNTQKMVKKSRRGPRSRSSQYRGVTFYRRTGRWESHIWDCGKQVYLGGFDTAYAAARAYDRAAIKFRGLDADINFVVDDYRHDIDKMKNLNKVEFVQT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F LQRNTQKMVKKSRRGPRSRSSQYRGVTFYRRTGRWESHIWDCGKQVYLGGFDTAYAAARAYDRAAIKFRGLDADINFVVDDYRHDIDKMKNLNKVEFVQT 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT3G54 LRRESASFGRGSSKYKGLALQKCTQFKTHDQIHLFQNRGWDAAAIKYNELGKGEGAMKFGAHIKGNGHNDLELSLGISSSSESIKLTTGDYYKGINRSTM :::::::::::::::::::::::::::::::::::::: . . IE_04F LRRESASFGRGSSKYKGLALQKCTQFKTHDQIHLFQNRYHFVRHVIF----------------------------------------------------- 210 220 230 240 310 320 330 340 AT3G54 GLYGKQSSIFLPMATMKPLKTVAASSGFPFISMTSSSSSMSNCFDP IE_04F ----------------------------------------------

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