◆◆◆IE_04F08 detail◆◆◆

Code name	IE_04F08
Locus	AT2G43010
Template	IT_04F08 (HR3438)
Donor Vector	pDONR207
Forward primer	IPF_04F08 (ggggacaagtttgtacaaaaaagcaggcttcATGGAACACCAAGGTTGGAGTTTTGAGG)
Reverse primer	IPR_04F08 (ggggaccactttgtacaagaaagctgggttGTGGTCCAAACGAGAACCGTCGGTGGTCTT)
Alignment with template seq.	1st	アミノ酸配列100%一致	2nd	-
Alignment with TAIR7CDS	1st	アミノ酸配列100%一致	2nd	-
comment	1st:Templateの配列とは一致
sequence	>IE_04F08_1290bp ATGGAACACCAAGGTTGGAGTTTTGAGGAGAATTATAGTTTGTCCACTAATAGAAGATCTATCAGGCCACAAGATGAACTAGTGGAGTTATTATGGCGAG ATGGACAAGTGGTTCTGCAGAGCCAAACTCATAGAGAACAAACCCAAACCCAGAAACAAGATCATCATGAAGAAGCCCTAAGATCCAGCACCTTTCTTGA AGATCAAGAAACTGTCTCTTGGATCCAATACCCTCCAGATGAAGACCCATTCGAACCCGACGACTTCTCCTCCCACTTCTTCTCAACCATGGATCCCCTC CAGAGACCAACCTCAGAGACGGTTAAGCCTAAGTCCAGTCCTGAACCTCCTCAAGTCATGGTTAAGCCTAAGGCCTGTCCTGACCCTCCTCCTCAAGTCA TGCCTCCTCCAAAATTTAGGTTAACAAATTCATCATCGGGGATTAGGGAAACAGAAATGGAACAGTACTCGGTAACGACCGTTGGACCTAGCCATTGCGG AAGCAACCCATCACAGAACGATCTCGATGTCTCAATGAGTCATGATCGAAGCAAAAACATAGAAGAAAAGCTTAATCCGAACGCAAGTTCCTCATCAGGT GGCTCCTCTGGTTGCAGCTTTGGCAAAGATATCAAAGAAATGGCTAGTGGAAGATGCATCACAACCGACCGTAAGAGAAAACGTATAAATCACACTGACG AATCTGTATCTCTATCAGATGCAATCGGTAACAAGTCGAACCAACGATCAGGATCAAACCGAAGGAGTCGAGCAGCTGAAGTTCATAATCTCTCCGAAAG GAGGAGGAGAGATAGGATCAATGAGAGAATGAAGGCTTTGCAAGAACTAATACCTCACTGCAGTAAAACTGATAAAGCTTCGATTTTAGACGAAGCCATA GATTATTTGAAATCACTTCAGTTACAGCTTCAAGTGATGTGGATGGGGAGTGGAATGGCGGCGGCGGCGGCTTCGGCTCCGATGATGTTCCCCGGAGTTC AACCTCAGCAGTTCATACGTCAGATACAGAGCCCGGTACAGTTACCTCGATTTCCGGTTATGGATCAGTCTGCAATTCAGAACAATCCCGGTTTAGTTTG CCAAAACCCGGTACAAAACCAGATCATCTCCGACCGGTTTGCTAGATACATCGGTGGGTTCCCACACATGCAGGCCGCGACTCAGATGCAGCCGATGGAG ATGTTGAGATTTAGTTCACCGGCGGGACAGCAAAGCCAACAACCGTCGTCTGTGCCGACGAAGACCACCGACGGTTCTCGTTTGGACCAC >IE_04F08_translated MEHQGWSFEENYSLSTNRRSIRPQDELVELLWRDGQVVLQSQTHREQTQTQKQDHHEEALRSSTFLEDQETVSWIQYPPDEDPFEPDDFSSHFFSTMDPL QRPTSETVKPKSSPEPPQVMVKPKACPDPPPQVMPPPKFRLTNSSSGIRETEMEQYSVTTVGPSHCGSNPSQNDLDVSMSHDRSKNIEEKLNPNASSSSG GSSGCSFGKDIKEMASGRCITTDRKRKRINHTDESVSLSDAIGNKSNQRSGSNRRSRAAEVHNLSERRRRDRINERMKALQELIPHCSKTDKASILDEAI DYLKSLQLQLQVMWMGSGMAAAAASAPMMFPGVQPQQFIRQIQSPVQLPRFPVMDQSAIQNNPGLVCQNPVQNQIISDRFARYIGGFPHMQAATQMQPME MLRFSSPAGQQSQQPSSVPTKTTDGSRLDH
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 1289/1290 (99.9%)（アミノ酸配列で100%）一致しました。 10 20 30 40 50 60 70 80 90 100 AT2G43 ATGGAACACCAAGGTTGGAGTTTTGAGGAGAATTATAGTTTGTCCACTAATAGAAGATCTATCAGGCCACAAGATGAACTAGTGGAGTTATTATGGCGAG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F ATGGAACACCAAGGTTGGAGTTTTGAGGAGAATTATAGTTTGTCCACTAATAGAAGATCTATCAGGCCACAAGATGAACTAGTGGAGTTATTATGGCGAG 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT2G43 ATGGACAAGTGGTTCTGCAGAGCCAAACTCATAGAGAACAAACCCAAACCCAGAAACAAGATCATCATGAAGAAGCCCTAAGATCCAGCACCTTTCTTGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F ATGGACAAGTGGTTCTGCAGAGCCAAACTCATAGAGAACAAACCCAAACCCAGAAACAAGATCATCATGAAGAAGCCCTAAGATCCAGCACCTTTCTTGA 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT2G43 AGATCAAGAAACTGTCTCTTGGATCCAATACCCTCCAGATGAAGACCCATTCGAACCCGACGACTTCTCCTCCCACTTCTTCTCAACCATGGATCCCCTC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F AGATCAAGAAACTGTCTCTTGGATCCAATACCCTCCAGATGAAGACCCATTCGAACCCGACGACTTCTCCTCCCACTTCTTCTCAACCATGGATCCCCTC 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 AT2G43 CAGAGACCAACCTCAGAGACGGTTAAGCCTAAGTCCAGTCCTGAACCTCCTCAAGTCATGGTTAAGCCTAAGGCCTGTCCTGACCCTCCTCCTCAAGTCA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F CAGAGACCAACCTCAGAGACGGTTAAGCCTAAGTCCAGTCCTGAACCTCCTCAAGTCATGGTTAAGCCTAAGGCCTGTCCTGACCCTCCTCCTCAAGTCA 310 320 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 480 490 500 AT2G43 TGCCTCCTCCAAAATTTAGGTTAACAAATTCATCATCGGGGATTAGGGAAACAGAAATGGAACAGTACTCGGTAACGACCGTTGGACCTAGCCATTGCGG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F TGCCTCCTCCAAAATTTAGGTTAACAAATTCATCATCGGGGATTAGGGAAACAGAAATGGAACAGTACTCGGTAACGACCGTTGGACCTAGCCATTGCGG 410 420 430 440 450 460 470 480 490 500 510 520 530 540 550 560 570 580 590 600 AT2G43 AAGCAACCCATCACAGAACGATCTCGATGTCTCAATGAGTCATGATCGAAGCAAAAACATAGAAGAAAAGCTTAATCCGAACGCAAGTTCCTCATCAGGT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F AAGCAACCCATCACAGAACGATCTCGATGTCTCAATGAGTCATGATCGAAGCAAAAACATAGAAGAAAAGCTTAATCCGAACGCAAGTTCCTCATCAGGT 510 520 530 540 550 560 570 580 590 600 610 620 630 640 650 660 670 680 690 700 AT2G43 GGCTCCTCTGGTTGCAGCTTTGGCAAAGATATCAAAGAAATGGCTAGTGGAAGATGCATCACAACCGACCGTAAGAGAAAACGTATAAATCACACTGACG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F GGCTCCTCTGGTTGCAGCTTTGGCAAAGATATCAAAGAAATGGCTAGTGGAAGATGCATCACAACCGACCGTAAGAGAAAACGTATAAATCACACTGACG 610 620 630 640 650 660 670 680 690 700 710 720 730 740 750 760 770 780 790 800 AT2G43 AATCTGTATCTCTATCAGATGCAATCGGTAACAAGTCGAACCAACGATCAGGATCAAACCGAAGGAGTCGAGCAGCTGAAGTTCATAATCTCTCCGAAAG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F AATCTGTATCTCTATCAGATGCAATCGGTAACAAGTCGAACCAACGATCAGGATCAAACCGAAGGAGTCGAGCAGCTGAAGTTCATAATCTCTCCGAAAG 710 720 730 740 750 760 770 780 790 800 810 820 830 840 850 860 870 880 890 900 AT2G43 GAGGAGGAGAGATAGGATCAATGAGAGAATGAAGGCTTTGCAAGAACTAATACCTCACTGCAGTAAAACTGATAAAGCTTCGATTTTAGACGAAGCCATA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F GAGGAGGAGAGATAGGATCAATGAGAGAATGAAGGCTTTGCAAGAACTAATACCTCACTGCAGTAAAACTGATAAAGCTTCGATTTTAGACGAAGCCATA 810 820 830 840 850 860 870 880 890 900 910 920 930 940 950 960 970 980 990 1000 AT2G43 GATTATTTGAAATCACTTCAGTTACAGCTTCAAGTGATGTGGATGGGGAGTGGAATGGCGGCGGCGGCGGCTTCGGCTCCGATGATGTTCCCCGGAGTTC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F GATTATTTGAAATCACTTCAGTTACAGCTTCAAGTGATGTGGATGGGGAGTGGAATGGCGGCGGCGGCGGCTTCGGCTCCGATGATGTTCCCCGGAGTTC 910 920 930 940 950 960 970 980 990 1000 1010 1020 1030 1040 1050 1060 1070 1080 1090 1100 AT2G43 AACCTCAGCAGTTCATACGTCAGATACAGAGCCCGGTACAGTTACCTCGATTTCCGGTTATGGATCAGTCTGCAATTCAGAACAATCCCGGTTTAGTTTG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F AACCTCAGCAGTTCATACGTCAGATACAGAGCCCGGTACAGTTACCTCGATTTCCGGTTATGGATCAGTCTGCAATTCAGAACAATCCCGGTTTAGTTTG 1010 1020 1030 1040 1050 1060 1070 1080 1090 1100 1110 1120 1130 1140 1150 1160 1170 1180 1190 1200 AT2G43 CCAAAACCCGGTACAAAACCAGATCATCTCCGACCGGTTTGCTAGATACATCGGTGGGTTCCCACACATGCAGGCCGCGACTCAGATGCAGCCGATGGAG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F CCAAAACCCGGTACAAAACCAGATCATCTCCGACCGGTTTGCTAGATACATCGGTGGGTTCCCACACATGCAGGCCGCGACTCAGATGCAGCCGATGGAG 1110 1120 1130 1140 1150 1160 1170 1180 1190 1200 1210 1220 1230 1240 1250 1260 1270 1280 1290 AT2G43 ATGTTGAGATTTAGTTCACCGGCGGGACAGCAAAGTCAACAACCGTCGTCTGTGCCGACGAAGACCACCGACGGTTCTCGTTTGGACCAC ::::::::::::::::::::::::::::::::::: :::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F ATGTTGAGATTTAGTTCACCGGCGGGACAGCAAAGCCAACAACCGTCGTCTGTGCCGACGAAGACCACCGACGGTTCTCGTTTGGACCAC 1210 1220 1230 1240 1250 1260 1270 1280 1290 10 20 30 40 50 60 70 80 90 100 AT2G43 MEHQGWSFEENYSLSTNRRSIRPQDELVELLWRDGQVVLQSQTHREQTQTQKQDHHEEALRSSTFLEDQETVSWIQYPPDEDPFEPDDFSSHFFSTMDPL :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F MEHQGWSFEENYSLSTNRRSIRPQDELVELLWRDGQVVLQSQTHREQTQTQKQDHHEEALRSSTFLEDQETVSWIQYPPDEDPFEPDDFSSHFFSTMDPL 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT2G43 QRPTSETVKPKSSPEPPQVMVKPKACPDPPPQVMPPPKFRLTNSSSGIRETEMEQYSVTTVGPSHCGSNPSQNDLDVSMSHDRSKNIEEKLNPNASSSSG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F QRPTSETVKPKSSPEPPQVMVKPKACPDPPPQVMPPPKFRLTNSSSGIRETEMEQYSVTTVGPSHCGSNPSQNDLDVSMSHDRSKNIEEKLNPNASSSSG 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT2G43 GSSGCSFGKDIKEMASGRCITTDRKRKRINHTDESVSLSDAIGNKSNQRSGSNRRSRAAEVHNLSERRRRDRINERMKALQELIPHCSKTDKASILDEAI :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F GSSGCSFGKDIKEMASGRCITTDRKRKRINHTDESVSLSDAIGNKSNQRSGSNRRSRAAEVHNLSERRRRDRINERMKALQELIPHCSKTDKASILDEAI 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 AT2G43 DYLKSLQLQLQVMWMGSGMAAAAASAPMMFPGVQPQQFIRQIQSPVQLPRFPVMDQSAIQNNPGLVCQNPVQNQIISDRFARYIGGFPHMQAATQMQPME :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F DYLKSLQLQLQVMWMGSGMAAAAASAPMMFPGVQPQQFIRQIQSPVQLPRFPVMDQSAIQNNPGLVCQNPVQNQIISDRFARYIGGFPHMQAATQMQPME 310 320 330 340 350 360 370 380 390 400 410 420 430 AT2G43 MLRFSSPAGQQSQQPSSVPTKTTDGSRLDH :::::::::::::::::::::::::::::: IE_04F MLRFSSPAGQQSQQPSSVPTKTTDGSRLDH 410 420 430

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