◆◆◆IE_04F09（IE_09B02） detail◆◆◆

Code nameIE_04F09（IE_09B02）
LocusAT3G59060
TemplateIT_04F09 (HR3445)
Donor VectorpDONR207
Forward primerIPF_04F09 (ggggacaagtttgtacaaaaaagcaggcttcATGGAACAAGTGTTTGCTGATTGGAATT)
Reverse primerIPR_04F09 (ggggaccactttgtacaagaaagctgggttGCCTATTTTACCCATATGAAGACTGTCGGT)
Alignment with template seq.1stアミノ酸配列99.80%一致2ndアミノ酸配列100%一致
Alignment with TAIR7CDSアミノ酸配列99.30%一致アミノ酸配列99.30%一致
comment1st:(Sequence解析まで行ったが、フレームはずれないが塩基置換がある)→2nd(09B02):Templateの配列とは一致, nt.764G→A
sequence 
>IE_04F09_1332bp
ATGGAACAAGTGTTTGCTGATTGGAATTTTGAAGATAATTTTCACATGTCCACTAATAAAAGATCAATCAGACCAGAAGATGAATTAGTGGAGCTATTGT
GGAGAGATGGTCAAGTGGTTTTACAAAGCCAAGCTCGTAGAGAACCGTCAGTCCAAGTCCAAACCCACAAACAAGAAACCCTAAGAAAACCCAACAATAT
TTTTCTTGACAACCAAGAAACAGTACAAAAGCCTAACTACGCTGCTCTAGATGATCAAGAAACCGTCTCCTGGATACAATACCCTCCGGATGACGTCATC
GACCCTTTCGAATCCGAGTTCTCCTCTCATTTCTTCTCTTCGATCGATCACCTCGGAGGTCCTGAGAAGCCACGAACGATCGAAGAGACAGTTAAGCATG
AGGCTCAAGCCATGGCTCCTCCTAAGTTTAGATCCTCGGTTATAACAGTCGGACCGAGTCATTGCGGCAGCAACCAGTCAACAAATATTCATCAGGCCAC
TACACTTCCGGTTTCTATGAGTGATAGAAGCAAGAACGTCGAAGAAAGACTTGACACTTCGTCAGGTGGCTCCTCCGGTTGCAGCTATGGAAGGAACAAC
AAAGAAACCGTTAGTGGAACAAGTGTAACCATTGACCGTAAAAGAAAACATGTTATGGATGCTGATCAAGAATCTGTGTCTCAATCAGATATAGGTTTGA
CCTCAACCGATGATCAAACCATGGGTAACAAATCGAGCCAACGGTCAGGATCTACTCGAAGAAGCCGTGCAGCTGAAGTTCATAATCTCTCAGAAAGGAG
GAGGAGAGATCGGATCAATGAAAGAATGAAAGCTCTTCAAGAACTCATACCTCACTGCAGCAGAACAGATAAAGCTTCGATATTGGATGAAGCAATTGAT
TACTTAAAATCACTTCAAATGCAACTCCAAGTGATGTGGATGGGAAGTGGAATGGCGGCGGCGGCAGCAGCAGCAGCAAGTCCGATGATGTTTCCCGGGG
TACAATCATCTCCATACATTAATCAGATGGCTATGCAAAGTCAGATGCAATTGTCTCAATTCCCGGTTATGAACCGGTCCGCTCCGCAGAACCATCCCGG
TTTAGTATGTCAAAACCCGGTACAGTTGCAGCTCCAAGCACAGAACCAAATCTTATCGGAGCAGCTCGCTAGGTACATGGGCGGGATTCCCCAGATGCCG
CCGGCGGGAAATCAGATGCAGACCGTGCAACAACAACCAGCGGACATGTTGGGATTTGGATCTCCGGCGGGACCGCAAAGTCAACTGTCGGCACCGGCGA
CCACCGACAGTCTTCATATGGGTAAAATAGGC

>IE_04F09_translated
MEQVFADWNFEDNFHMSTNKRSIRPEDELVELLWRDGQVVLQSQARREPSVQVQTHKQETLRKPNNIFLDNQETVQKPNYAALDDQETVSWIQYPPDDVI
DPFESEFSSHFFSSIDHLGGPEKPRTIEETVKHEAQAMAPPKFRSSVITVGPSHCGSNQSTNIHQATTLPVSMSDRSKNVEERLDTSSGGSSGCSYGRNN
KETVSGTSVTIDRKRKHVMDADQESVSQSDIGLTSTDDQTMGNKSSQRSGSTRRSRAAEVHNLSERRRRDRINERMKALQELIPHCSRTDKASILDEAID
YLKSLQMQLQVMWMGSGMAAAAAAAASPMMFPGVQSSPYINQMAMQSQMQLSQFPVMNRSAPQNHPGLVCQNPVQLQLQAQNQILSEQLARYMGGIPQMP
PAGNQMQTVQQQPADMLGFGSPAGPQSQLSAPATTDSLHMGKIG
Alignment ◆CDS（TAIRver.9）_without_stop_codonとのalignment◆

1326/1332 (99.5%)（アミノ酸配列で442/444 (99.5%)）一致しました。


               10        20        30        40        50        60        70        80        90       100
AT3G59 ATGGAACAAGTGTTTGCTGATTGGAATTTTGAAGATAATTTTCACATGTCCACTAATAAAAGATCAATCAGACCAGAAGATGAATTAGTGGAGCTATTGT
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_04F ATGGAACAAGTGTTTGCTGATTGGAATTTTGAAGATAATTTTCACATGTCCACTAATAAAAGATCAATCAGACCAGAAGATGAATTAGTGGAGCTATTGT
               10        20        30        40        50        60        70        80        90       100

              110       120       130       140       150       160       170       180       190       200
AT3G59 GGAGAGATGGTCAAGTGGTTTTACAAAGCCAAGCTCGTAGAGAACCGTCAGTCCAAGTCCAAACCCACAAACAAGAAACCCTAAGAAAACCCAACAATAT
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_04F GGAGAGATGGTCAAGTGGTTTTACAAAGCCAAGCTCGTAGAGAACCGTCAGTCCAAGTCCAAACCCACAAACAAGAAACCCTAAGAAAACCCAACAATAT
              110       120       130       140       150       160       170       180       190       200

              210       220       230       240       250       260       270       280       290       300
AT3G59 TTTTCTTGACAACCAAGAAACAGTACAAAAGCCTAACTACGCTGCTCTAGATGATCAAGAAACCGTCTCCTGGATACAATACCCTCCGGATGACGTCATC
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_04F TTTTCTTGACAACCAAGAAACAGTACAAAAGCCTAACTACGCTGCTCTAGATGATCAAGAAACCGTCTCCTGGATACAATACCCTCCGGATGACGTCATC
              210       220       230       240       250       260       270       280       290       300

              310       320       330       340       350       360       370       380       390       400
AT3G59 GACCCTTTCGAATCCGAGTTCTCCTCTCATTTCTTCTCTTCGATCGATCACCTCGGAGGTCCTGAGAAGCCACGAACGATCGAAGAGACAGTTAAGCATG
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_04F GACCCTTTCGAATCCGAGTTCTCCTCTCATTTCTTCTCTTCGATCGATCACCTCGGAGGTCCTGAGAAGCCACGAACGATCGAAGAGACAGTTAAGCATG
              310       320       330       340       350       360       370       380       390       400

              410       420       430       440       450       460       470       480       490       500
AT3G59 AGGCTCAAGCCATGGCTCCTCCTAAGTTTAGATCCTCGGTTATAACAGTCGGACCGAGTCATTGCGGCAGCAACCAGTCAACAAATATTCATCAGGCCAC
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_04F AGGCTCAAGCCATGGCTCCTCCTAAGTTTAGATCCTCGGTTATAACAGTCGGACCGAGTCATTGCGGCAGCAACCAGTCAACAAATATTCATCAGGCCAC
              410       420       430       440       450       460       470       480       490       500

              510       520       530       540       550       560       570       580       590       600
AT3G59 TACACTTCCGGTTTCTATGAGTGATAGAAGCAAGAACGTCGAAGAAAGACTTGACACTTCGTCAGGTGGCTCCTCCGGTTGCAGCTATGGAAGGAACAAC
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_04F TACACTTCCGGTTTCTATGAGTGATAGAAGCAAGAACGTCGAAGAAAGACTTGACACTTCGTCAGGTGGCTCCTCCGGTTGCAGCTATGGAAGGAACAAC
              510       520       530       540       550       560       570       580       590       600

              610       620       630       640       650       660       670       680       690       700
AT3G59 AAAGAAACCGTTAGTGGAACAAGTGTAACCATTGACCGTAAAAGAAAACATGTTATGGATGCTGATCAAGAATCTGTGTCTCAATCAGATATAGGTTTGA
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_04F AAAGAAACCGTTAGTGGAACAAGTGTAACCATTGACCGTAAAAGAAAACATGTTATGGATGCTGATCAAGAATCTGTGTCTCAATCAGATATAGGTTTGA
              610       620       630       640       650       660       670       680       690       700

              710       720       730       740       750       760       770       780       790       800
AT3G59 CCTCAACCGATGATCAAACCATGGGTAACAAATCGAGCCAACGGTCAGGATCTACTCGAAGAAGCCGTGCAGCTGAAGTTCATAATCTCTCAGAAAGGAG
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_04F CCTCAACCGATGATCAAACCATGGGTAACAAATCGAGCCAACGGTCAGGATCTACTCGAAGAAGCCGTGCAGCTGAAGTTCATAATCTCTCAGAAAGGAG
              710       720       730       740       750       760       770       780       790       800

              810       820       830       840       850       860       870       880       890       900
AT3G59 GAGGAGAGATCGGATCAATGAAAGAATGAAAGCTCTTCAAGAACTCATACCTCACTGCAGCAGAACAGATAAAGCTTCGATATTGGATGAAGCAATTGAT
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_04F GAGGAGAGATCGGATCAATGAAAGAATGAAAGCTCTTCAAGAACTCATACCTCACTGCAGCAGAACAGATAAAGCTTCGATATTGGATGAAGCAATTGAT
              810       820       830       840       850       860       870       880       890       900

              910       920       930       940       950       960       970       980       990      1000
AT3G59 TACTTAAAATCACTTCAAATGCAACTCCAAGTGATGTGGATGGGAAGTGGAATGGCGGCGGCGGCAGCAGCAGCAGCAAGTCCGATGATGTTTCCCGGGG
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_04F TACTTAAAATCACTTCAAATGCAACTCCAAGTGATGTGGATGGGAAGTGGAATGGCGGCGGCGGCAGCAGCAGCAGCAAGTCCGATGATGTTTCCCGGGG
              910       920       930       940       950       960       970       980       990      1000

             1010      1020      1030      1040      1050      1060      1070      1080      1090      1100
AT3G59 TACAATCATCTCCATACATTAATCAGATGGCTATGCAAAGTCAGATGCAATTGTCTCAATTCCCGGTTATGAACCGGTCCGCTCCGCAGAACCATCCCGG
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_04F TACAATCATCTCCATACATTAATCAGATGGCTATGCAAAGTCAGATGCAATTGTCTCAATTCCCGGTTATGAACCGGTCCGCTCCGCAGAACCATCCCGG
             1010      1020      1030      1040      1050      1060      1070      1080      1090      1100

             1110      1120      1130      1140      1150      1160      1170      1180      1190      1200
AT3G59 TTTAGTATGTCAAAACCCGGTACAGTTGCAGCTCCAAGCACAGAACCAAATCTTATCGGAGCAGCTCGCTAGGTACATGGGCGGGATTCCCCAGATGCCG
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_04F TTTAGTATGTCAAAACCCGGTACAGTTGCAGCTCCAAGCACAGAACCAAATCTTATCGGAGCAGCTCGCTAGGTACATGGGCGGGATTCCCCAGATGCCG
             1110      1120      1130      1140      1150      1160      1170      1180      1190      1200

             1210            1220      1230      1240      1250      1260      1270      1280      1290    
AT3G59 CCGGCGGGAAAT------CAGACCGTGCAACAACAACCAGCGGACATGTTGGGATTTGGATCTCCGGCGGGACCGCAAAGTCAACTGTCGGCACCGGCGA
       ::::::::::::      ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_04F CCGGCGGGAAATCAGATGCAGACCGTGCAACAACAACCAGCGGACATGTTGGGATTTGGATCTCCGGCGGGACCGCAAAGTCAACTGTCGGCACCGGCGA
             1210      1220      1230      1240      1250      1260      1270      1280      1290      1300

         1300      1310      1320      
AT3G59 CCACCGACAGTCTTCATATGGGTAAAATAGGC
       ::::::::::::::::::::::::::::::::
IE_04F CCACCGACAGTCTTCATATGGGTAAAATAGGC
             1310      1320      1330  




               10        20        30        40        50        60        70        80        90       100
AT3G59 MEQVFADWNFEDNFHMSTNKRSIRPEDELVELLWRDGQVVLQSQARREPSVQVQTHKQETLRKPNNIFLDNQETVQKPNYAALDDQETVSWIQYPPDDVI
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_04F MEQVFADWNFEDNFHMSTNKRSIRPEDELVELLWRDGQVVLQSQARREPSVQVQTHKQETLRKPNNIFLDNQETVQKPNYAALDDQETVSWIQYPPDDVI
               10        20        30        40        50        60        70        80        90       100

              110       120       130       140       150       160       170       180       190       200
AT3G59 DPFESEFSSHFFSSIDHLGGPEKPRTIEETVKHEAQAMAPPKFRSSVITVGPSHCGSNQSTNIHQATTLPVSMSDRSKNVEERLDTSSGGSSGCSYGRNN
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_04F DPFESEFSSHFFSSIDHLGGPEKPRTIEETVKHEAQAMAPPKFRSSVITVGPSHCGSNQSTNIHQATTLPVSMSDRSKNVEERLDTSSGGSSGCSYGRNN
              110       120       130       140       150       160       170       180       190       200

              210       220       230       240       250       260       270       280       290       300
AT3G59 KETVSGTSVTIDRKRKHVMDADQESVSQSDIGLTSTDDQTMGNKSSQRSGSTRRSRAAEVHNLSERRRRDRINERMKALQELIPHCSRTDKASILDEAID
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_04F KETVSGTSVTIDRKRKHVMDADQESVSQSDIGLTSTDDQTMGNKSSQRSGSTRRSRAAEVHNLSERRRRDRINERMKALQELIPHCSRTDKASILDEAID
              210       220       230       240       250       260       270       280       290       300

              310       320       330       340       350       360       370       380       390       400
AT3G59 YLKSLQMQLQVMWMGSGMAAAAAAAASPMMFPGVQSSPYINQMAMQSQMQLSQFPVMNRSAPQNHPGLVCQNPVQLQLQAQNQILSEQLARYMGGIPQMP
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_04F YLKSLQMQLQVMWMGSGMAAAAAAAASPMMFPGVQSSPYINQMAMQSQMQLSQFPVMNRSAPQNHPGLVCQNPVQLQLQAQNQILSEQLARYMGGIPQMP
              310       320       330       340       350       360       370       380       390       400

                410       420       430       440  
AT3G59 PAGN--QTVQQQPADMLGFGSPAGPQSQLSAPATTDSLHMGKIG
       ::::  ::::::::::::::::::::::::::::::::::::::
IE_04F PAGNQMQTVQQQPADMLGFGSPAGPQSQLSAPATTDSLHMGKIG
              410       420       430       440

このページのTOPに戻る