◆◆◆IE_04F10 detail◆◆◆

Code name	IE_04F10
Locus	AT5G61270
Template	IT_04F10 (HR3458)
Donor Vector	pDONR207
Forward primer	IPF_04F10 (ggggacaagtttgtacaaaaaagcaggcttcATGTCGAATTATGGAGTTAAAGAGCTCA)
Reverse primer	IPR_04F10 (ggggaccactttgtacaagaaagctgggttATCTCTTTTCTCATGATTCGAAGAACTTGA)
Alignment with template seq.	1st	アミノ酸配列100%一致	2nd	-
Alignment with TAIR7CDS	1st	アミノ酸配列100%一致	2nd	-
comment	1st:Templateの配列とは一致
sequence	>IE_04F10_1098bp ATGTCGAATTATGGAGTTAAAGAGCTCACATGGGAAAATGGGCAACTAACCGTTCATGGTCTAGGCGACGAAGTAGAACCAACCACCTCGAATAACCCTA TTTGGACTCAAAGTCTCAACGGTTGTGAGACTTTGGAGTCTGTGGTTCATCAAGCGGCTCTACAGCAGCCAAGCAAGTTTCAGCTGCAGAGTCCGAATGG TCCAAACCACAATTATGAGAGCAAGGATGGATCTTGTTCAAGAAAACGCGGTTATCCTCAAGAAATGGACCGATGGTTCGCTGTTCAAGAGGAGAGCCAT AGAGTTGGCCACAGCGTCACTGCAAGTGCGAGTGGTACCAATATGTCTTGGGCGTCTTTTGAATCCGGTCGGAGCTTGAAGACAGCTAGAACCGGAGACA GAGACTATTTCCGCTCTGGATCGGAAACTCAAGATACTGAAGGAGATGAACAAGAGACAAGAGGAGAAGCAGGTAGATCTAATGGACGACGGGGACGAGC AGCAGCGATTCACAACGAGTCCGAAAGGAGACGGCGTGATAGGATAAACCAGAGGATGAGAACACTTCAGAAGCTGCTTCCTACTGCAAGTAAGGCGGAT AAAGTCTCAATCTTGGATGATGTTATCGAACACTTGAAACAGCTACAAGCACAGGTACAGTTCATGAGCCTAAGAGCCAACTTGCCACAACAAATGATGA TTCCGCAACTACCTCCACCACAGTCAGTTCTCAGCATCCAACACCAACAACAACAACAACAACAGCAGCAGCAGCAGCAACAACAGCAGCAACAGTTTCA GATGTCGTTGCTTGCAACAATGGCAAGAATGGGAATGGGAGGTGGTGGAAATGGTTATGGAGGTTTAGTTCCTCCTCCTCCTCCTCCACCAATGATGGTC CCTCCTATGGGTAACAGAGACTGCACCAACGGTTCTTCAGCCACATTATCTGATCCATACAGCGCCTTTTTCGCACAGACAATGAATATGGATCTCTACA ATAAAATGGCAGCAGCTATCTATAGGCAACAGTCTGATCAAACAACAAAGGTAAATATCGGCATGCCCTCAAGTTCTTCGAATCATGAGAAAAGAGAT >IE_04F10_translated MSNYGVKELTWENGQLTVHGLGDEVEPTTSNNPIWTQSLNGCETLESVVHQAALQQPSKFQLQSPNGPNHNYESKDGSCSRKRGYPQEMDRWFAVQEESH RVGHSVTASASGTNMSWASFESGRSLKTARTGDRDYFRSGSETQDTEGDEQETRGEAGRSNGRRGRAAAIHNESERRRRDRINQRMRTLQKLLPTASKAD KVSILDDVIEHLKQLQAQVQFMSLRANLPQQMMIPQLPPPQSVLSIQHQQQQQQQQQQQQQQQQQFQMSLLATMARMGMGGGGNGYGGLVPPPPPPPMMV PPMGNRDCTNGSSATLSDPYSAFFAQTMNMDLYNKMAAAIYRQQSDQTTKVNIGMPSSSSNHEKRD
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 1095/1098 (99.7%)（アミノ酸配列で100%）一致しました。 10 20 30 40 50 60 70 80 90 100 AT5G61 ATGTCGAATTATGGAGTTAAAGAGCTCACATGGGAAAATGGGCAACTAACCGTTCATGGTCTAGGCGACGAAGTAGAACCAACCACCTCGAATAACCCTA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F ATGTCGAATTATGGAGTTAAAGAGCTCACATGGGAAAATGGGCAACTAACCGTTCATGGTCTAGGCGACGAAGTAGAACCAACCACCTCGAATAACCCTA 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT5G61 TTTGGACTCAAAGTCTCAACGGTTGTGAGACTTTGGAGTCTGTGGTTCATCAAGCGGCTCTACAGCAGCCAAGCAAGTTTCAGCTGCAGAGTCCGAATGG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F TTTGGACTCAAAGTCTCAACGGTTGTGAGACTTTGGAGTCTGTGGTTCATCAAGCGGCTCTACAGCAGCCAAGCAAGTTTCAGCTGCAGAGTCCGAATGG 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT5G61 TCCAAACCACAATTATGAGAGCAAGGATGGATCTTGTTCAAGAAAACGCGGTTATCCTCAAGAAATGGACCGATGGTTCGCTGTTCAAGAGGAGAGCCAT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F TCCAAACCACAATTATGAGAGCAAGGATGGATCTTGTTCAAGAAAACGCGGTTATCCTCAAGAAATGGACCGATGGTTCGCTGTTCAAGAGGAGAGCCAT 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 AT5G61 AGAGTTGGCCACAGCGTCACTGCAAGTGCGAGTGGTACCAATATGTCTTGGGCGTCTTTTGAATCCGGTCGGAGCTTGAAGACAGCTAGAACCGGAGACA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F AGAGTTGGCCACAGCGTCACTGCAAGTGCGAGTGGTACCAATATGTCTTGGGCGTCTTTTGAATCCGGTCGGAGCTTGAAGACAGCTAGAACCGGAGACA 310 320 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 480 490 500 AT5G61 GAGACTATTTCCGCTCTGGATCGGAAACTCAAGATACTGAAGGAGATGAACAAGAGACAAGAGGAGAAGCAGGTAGATCTAATGGACGACGGGGACGAGC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F GAGACTATTTCCGCTCTGGATCGGAAACTCAAGATACTGAAGGAGATGAACAAGAGACAAGAGGAGAAGCAGGTAGATCTAATGGACGACGGGGACGAGC 410 420 430 440 450 460 470 480 490 500 510 520 530 540 550 560 570 580 590 600 AT5G61 AGCAGCGATTCACAACGAGTCCGAAAGGAGACGGCGTGATAGGATAAACCAGAGGATGAGAACACTTCAGAAGCTGCTTCCTACTGCAAGTAAGGCGGAT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F AGCAGCGATTCACAACGAGTCCGAAAGGAGACGGCGTGATAGGATAAACCAGAGGATGAGAACACTTCAGAAGCTGCTTCCTACTGCAAGTAAGGCGGAT 510 520 530 540 550 560 570 580 590 600 610 620 630 640 650 660 670 680 690 700 AT5G61 AAAGTCTCAATCTTGGATGATGTTATCGAACACTTGAAACAGCTACAAGCACAAGTACAGTTCATGAGCCTAAGAGCCAACTTGCCACAACAAATGATGA ::::::::::::::::::::::::::::::::::::::::::::::::::::: :::::::::::::::::::::::::::::::::::::::::::::: IE_04F AAAGTCTCAATCTTGGATGATGTTATCGAACACTTGAAACAGCTACAAGCACAGGTACAGTTCATGAGCCTAAGAGCCAACTTGCCACAACAAATGATGA 610 620 630 640 650 660 670 680 690 700 710 720 730 740 750 760 770 780 790 800 AT5G61 TTCCGCAACTACCTCCACCACAGTCAGTTCTCAGCATCCAACACCAACAACAACAACAACAACAGCAGCAGCAGCAGCAACAACAGCAGCAACAGTTTCA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F TTCCGCAACTACCTCCACCACAGTCAGTTCTCAGCATCCAACACCAACAACAACAACAACAACAGCAGCAGCAGCAGCAACAACAGCAGCAACAGTTTCA 710 720 730 740 750 760 770 780 790 800 810 820 830 840 850 860 870 880 890 900 AT5G61 GATGTCGTTGCTTGCAACAATGGCAAGAATGGGAATGGGAGGTGGTGGAAATGGTTATGGAGGTTTAGTTCCTCCTCCTCCTCCTCCACCAATGATGGTC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F GATGTCGTTGCTTGCAACAATGGCAAGAATGGGAATGGGAGGTGGTGGAAATGGTTATGGAGGTTTAGTTCCTCCTCCTCCTCCTCCACCAATGATGGTC 810 820 830 840 850 860 870 880 890 900 910 920 930 940 950 960 970 980 990 1000 AT5G61 CCTCCTATGGGTAACAGAGACTGCACCAACGGTTCTTCAGCCACATTATCTGATCCATACAGCGCCTTTTTCGCACAGACAATGAATATGGATCTCTACA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F CCTCCTATGGGTAACAGAGACTGCACCAACGGTTCTTCAGCCACATTATCTGATCCATACAGCGCCTTTTTCGCACAGACAATGAATATGGATCTCTACA 910 920 930 940 950 960 970 980 990 1000 1010 1020 1030 1040 1050 1060 1070 1080 1090 AT5G61 ATAAAATGGCAGCAGCTATCTATAGACAACAGTCTGATCAAACAACAAAGGTAAATATCGGCATGCCTTCAAGTTCTTCGAATCATGAGAAAAGAGAT ::::::::::::::::::::::::: ::::::::::::::::::::::::::::::::::::::::: :::::::::::::::::::::::::::::: IE_04F ATAAAATGGCAGCAGCTATCTATAGGCAACAGTCTGATCAAACAACAAAGGTAAATATCGGCATGCCCTCAAGTTCTTCGAATCATGAGAAAAGAGAT 1010 1020 1030 1040 1050 1060 1070 1080 1090 10 20 30 40 50 60 70 80 90 100 AT5G61 MSNYGVKELTWENGQLTVHGLGDEVEPTTSNNPIWTQSLNGCETLESVVHQAALQQPSKFQLQSPNGPNHNYESKDGSCSRKRGYPQEMDRWFAVQEESH :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F MSNYGVKELTWENGQLTVHGLGDEVEPTTSNNPIWTQSLNGCETLESVVHQAALQQPSKFQLQSPNGPNHNYESKDGSCSRKRGYPQEMDRWFAVQEESH 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT5G61 RVGHSVTASASGTNMSWASFESGRSLKTARTGDRDYFRSGSETQDTEGDEQETRGEAGRSNGRRGRAAAIHNESERRRRDRINQRMRTLQKLLPTASKAD :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F RVGHSVTASASGTNMSWASFESGRSLKTARTGDRDYFRSGSETQDTEGDEQETRGEAGRSNGRRGRAAAIHNESERRRRDRINQRMRTLQKLLPTASKAD 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT5G61 KVSILDDVIEHLKQLQAQVQFMSLRANLPQQMMIPQLPPPQSVLSIQHQQQQQQQQQQQQQQQQQFQMSLLATMARMGMGGGGNGYGGLVPPPPPPPMMV :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F KVSILDDVIEHLKQLQAQVQFMSLRANLPQQMMIPQLPPPQSVLSIQHQQQQQQQQQQQQQQQQQFQMSLLATMARMGMGGGGNGYGGLVPPPPPPPMMV 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 AT5G61 PPMGNRDCTNGSSATLSDPYSAFFAQTMNMDLYNKMAAAIYRQQSDQTTKVNIGMPSSSSNHEKRD :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04F PPMGNRDCTNGSSATLSDPYSAFFAQTMNMDLYNKMAAAIYRQQSDQTTKVNIGMPSSSSNHEKRD 310 320 330 340 350 360

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