◆◆◆IE_04F11 detail◆◆◆

Code nameIE_04F11
LocusAT1G11490
TemplateIT_04F11 (HR3464)
Donor VectorpDONR207
Forward primerIPF_04F11 (ggggacaagtttgtacaaaaaagcaggcttcATGCGTGGGGTTTGGCTTTATCTCAAGA)
Reverse primerIPR_04F11 (ggggaccactttgtacaagaaagctgggttTGGCTTGAAGATTATCACAAAGCAAGGTAA)
Alignment with template seq.1stアミノ酸配列100%一致2nd-
Alignment with TAIR7CDSアミノ酸配列100%一致-
comment
sequence 
>IE_04F11_1095bp
ATGCGTGGGGTTTGGCTTTATCTCAAGAAGTCTCTTAGCTGCTGCAAGGCACAGAAGTCAACTGTAGCTTGTGATCCAGACAAGATCCAAATTCAGAAGA
AGGAGAATCCATCTGGATGCTCAAGAAGATCAATGTCGAATCTGAGAGATGTCTTTGTAACTAATGGAGATGAAGGAGCTATGCAGAATGTAAGCTGCTG
CAGCTCACGGTCTCTTGAGAGCTCCAGGTTTGTAAACACAGTGAAATTTGAGGAGAATGCAGGTTATTCAGACAGATTCAAAGGTTTGCTTAGCGGAGCA
TCTTCTTCTGATTTACTTCCCGGTCGTTGTTCAGAGAGATTTGATGTTGTTGGCTCTGACATCTGCGGTTTCGGAGTTCTTGCTTGTCAAAAATGTCATG
AGAGAGTCAGAGATCTTGACGCGTTTGAAGCACATTACCTCTCTAACCACTCTGTCGTTAGACTCTTAGCAGGAGATTTCTCAAGAACAACTGTGGAACT
AATCTGCAACACAGGCTATTCTCACAAGCTAGGCAAAATGAAAGGGAACAACATTTCAGCGATCTTTAAAATCCAGAACTTGCAGAGAGTTGTTGCAGAC
TTTGAAGATTACAGAGAGCTCGTGAAAATAAGAGCAAACAAGCTTTCAAAGAAGCACTCTCGTTGTATGGCTGATGGCAACGAGTTTCTTGGGTTCCACG
GTACAACTCTTTCTTGCACTCTCGGTTTCAGTAACAGCTCTTCAAACCTCTGTTTCTCGGATCACTGTGAAGTTTGTCATATTCTAAGACATGGTTTCAG
TCCTAAAACAAGACCAGATGGGATAAAAGGAGTTCTCACAGCATCAACTAGCTCTACAGCTCTTGAATCTATCGAAACCGATCAAGGAAGAAATAGAGGT
TCATTGATAGCTGTTGTGCTATGTAGAGTGATTGCAGGGAGGGTTCATAAACCTATGCAGACGTTTGAAAATTCTTTAGGGTTTTCTGAGTTTGATTCAC
TAGCTTTGAAAGTTGGACAAAATTCAAGAATTGAAGAGCTTTACCTGTTGAGTACAAAAGCTTTGTTACCTTGCTTTGTGATAATCTTCAAGCCA

>IE_04F11_translated
MRGVWLYLKKSLSCCKAQKSTVACDPDKIQIQKKENPSGCSRRSMSNLRDVFVTNGDEGAMQNVSCCSSRSLESSRFVNTVKFEENAGYSDRFKGLLSGA
SSSDLLPGRCSERFDVVGSDICGFGVLACQKCHERVRDLDAFEAHYLSNHSVVRLLAGDFSRTTVELICNTGYSHKLGKMKGNNISAIFKIQNLQRVVAD
FEDYRELVKIRANKLSKKHSRCMADGNEFLGFHGTTLSCTLGFSNSSSNLCFSDHCEVCHILRHGFSPKTRPDGIKGVLTASTSSTALESIETDQGRNRG
SLIAVVLCRVIAGRVHKPMQTFENSLGFSEFDSLALKVGQNSRIEELYLLSTKALLPCFVIIFKP
Alignment ◆CDS（TAIRver.9）_without_stop_codonとのalignment◆

100%一致しました。

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