◆◆◆IE_04G04（IE_09B03） detail◆◆◆

Code nameIE_04G04（IE_09B03）
LocusAT1G02220
TemplateIT_04G04 (HR3555)
Donor VectorpDONR207
Forward primerIPF_04G04 (ggggacaagtttgtacaaaaaagcaggcttcATGGAAACTCCTGTGGGTTTAAGATTCT)
Reverse primerIPR_04G04 (ggggaccactttgtacaagaaagctgggttAGTTCTTGAGATGGAAGAACATAGGTGAAT)
Alignment with template seq.1st配列解析が行えなかったサンプルです。2ndアミノ酸配列100%一致
Alignment with TAIR7CDS-アミノ酸配列100%一致
comment1st:(PCRで目的サイズの増幅が確認できたが、コロニーが得られなかった)→2nd(09B03):100%
sequence 
>IE_04G04_1182bp
ATGGAAACTCCTGTGGGTTTAAGATTCTGTCCGACCGACGAGGAGATCGTCGTCGATTACCTTTGGCCGAAAAATTCCGATAGAGACACGAGCCATGTCG
ATCGATTCATTAACACAGTCCCTGTCTGTAGACTCGATCCTTGGGAGTTACCTTGCCAGTCAAGGATCAAACTGAAAGATGTGGCTTGGTGTTTCTTCAG
ACCTAAGGAGAACAAATATGGCAGAGGTGATCAGCAGATGAGAAAAACGAAATCTGGGTTTTGGAAGAGTACTGGCAGACCAAAGCCTATCATGCGTAAT
CGCCAACAGATCGGTGAGAAAAAGATTTTGATGTTTTACACGAGTAAGGAATCCAAATCCGATTGGGTTATACACGAGTACCACGGTTTCTCTCATAACC
AGATGATGATGACATACACACTCTGTAAAGTTATGTTTAATGGTGGCATGAGAGAGAAGTCTTCCTCTTCTCCTTCTTCTTCTGGTGTTAGTGGAATTGA
GCAGAGTCGTCGTGACTCTTTAATCCCTCAGCTTGTCAACAATTCTGAGGGATCCTCACTTCACAGAGAAGATCCAAGTCAGTTTGGTGATGTGCTGCAA
GAAGCTCCAATCGAGGATGCTAAACTGACCGAGGAATTGGTAAAATGGCTGATGAATGATGAGGATGATGCTCAAATCGAGGATGCTATACCGATTGAGG
AATGGGAAACATGGTTGAATGATATTGATGATGCTAAGGAGAAGAGTATCATGTTTATGCATGATAATCGAAGTGATTACAGACCTCCAAACTCATTAAC
TGGTGTCTTCAGTGATGATGTTAGCAGTGATGATAATGATTCTGATTTGCTAACTCCAAAAACAAACTCTATTCAAACTTCGAGCACTTGTGATAGTTTT
GGTAGCTCAAATCATCGCATAGACCAGATCAAAGACCTGCAAGAATCTCCTACCTCAACAATCAACTTAGTGTCACTAACTCAAGAGGTGAGCCAAGCTC
TAATAACCAGTATTGATACCGCCGAGAAGAAGAAGAATCCTTATGATGATGCACAAGGGACTGAGATTGGTGAGCATAAATTGGGTCAAGAGACGATCAA
GAAGAAAAGAGCTGGTTTCTTTCACAGGATGATACAAAAATTCGTCAAGAAAATTCACCTATGTTCTTCCATCTCAAGAACT

>IE_04G04_translated
METPVGLRFCPTDEEIVVDYLWPKNSDRDTSHVDRFINTVPVCRLDPWELPCQSRIKLKDVAWCFFRPKENKYGRGDQQMRKTKSGFWKSTGRPKPIMRN
RQQIGEKKILMFYTSKESKSDWVIHEYHGFSHNQMMMTYTLCKVMFNGGMREKSSSSPSSSGVSGIEQSRRDSLIPQLVNNSEGSSLHREDPSQFGDVLQ
EAPIEDAKLTEELVKWLMNDEDDAQIEDAIPIEEWETWLNDIDDAKEKSIMFMHDNRSDYRPPNSLTGVFSDDVSSDDNDSDLLTPKTNSIQTSSTCDSF
GSSNHRIDQIKDLQESPTSTINLVSLTQEVSQALITSIDTAEKKKNPYDDAQGTEIGEHKLGQETIKKKRAGFFHRMIQKFVKKIHLCSSISRT
Alignment ◆CDS（TAIRver.9）_without_stop_codonとのalignment◆

100%一致しました。

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