◆◆◆IE_04H04（IE_09B04） detail◆◆◆

Code nameIE_04H04（IE_09B04）
LocusAT5G18680
TemplateIT_04H04 (HR3748)
Donor VectorpDONR207
Forward primerIPF_04H04 (ggggacaagtttgtacaaaaaagcaggcttcATGACCTTACGTAGCTTAATCCTTGAGA)
Reverse primerIPR_04H04 (ggggaccactttgtacaagaaagctgggttTTCACAAGCGATTCTAGTCTCAAAGCTGCT)
Alignment with template seq.1stアミノ酸配列99.70%一致2ndアミノ酸配列100%一致
Alignment with TAIR7CDSアミノ酸配列99.70%一致アミノ酸配列100%一致
comment1st:(Sequence解析まで行ったが、フレームはずれないが塩基置換がある)→2nd(09B04):100%
sequence 
>IE_04H04_1167bp
ATGACCTTACGTAGCTTAATCCTTGAGATGCGTTCGAGACCGCATCGTGTGGTCCACGACCTTGCCGCCGCCGCAGCTGCCGATTCCACTTCTGTGTCAT
CGCAAGATTATCGCTGGTCAGAGATTCCTGAAGAGCTTCTTAGGGAGATTCTGATTCGTGTTGAAGCGGCGGACGGTGGCGGATGGCCGTCACGACGCAG
CGTGGTGGCTTGTGCCGGCGTTTGTCGTGGCTGGCGGCTACTTATGAACGAAACCGTCGTTGTCCCTGAGATCTCTTCTAAGTTGACTTTCCCCATCTCT
CTCAAGCAGCCTGGTCCAAGGGATTCACTGGTTCAATGCTTTATCAAACGTAATCGAATTACGCAATCATATCATCTCTATCTCGGATTAACCAACTCTT
TAACGGATGATGGGAAGTTTTTGCTTGCTGCGTGTAAGTTGAAGCACACAACTTGTACGGATTACATTATCTCTTTACGTTCTGATGATATGTCGAGAAG
AAGCCAAGCTTATGTTGGCAAAGTGAGATCGAACTTCCTAGGAACGAAATTCACTGTCTTTGATGGAAATCTGCTGCCTTCAACGGGAGCCGCAAAGTTG
AGAAAGAGCCGATCTTATAATCCCGCAAAAGTTTCAGCAAAAGTTCCTCTTGGAAGTTATCCTGTCGCTCATATCACATATGAGCTGAATGTCTTAGGAT
CCCGGGGACCAAGAAAGATGCAATGTCTTATGGACACAATACCTACAAGCACAATGGAGCCTCAAGGAGTAGCTTCAGAACCATCAGAGTTTCCCTTACT
CGGTACTCGGTCAACCTTATCCAGGTCTCAGTCAAAACCATTACGCAGTAGCTCAAGCCACCTGAAAGAAACACCATTAGTGCTGAGCAACAAGACACCA
CGGTGGCACGAGCAGCTACGCTGCTGGTGCTTGAATTTCCATGGCCGTGTCACAGTAGCGTCAGTGAAGAACTTTCAGCTCGTGGCAGCAGGAGCTAGCT
GTGGCAGTGGCACGGGAATGTCACCGGAGAGGCAGAGCGAGCGGATTATATTGCAGTTTGGGAAAGTCGGGAAAGATATGTTCACGATGGATTATGGATA
CCCGATCTCAGCTTTCCAGGCTTTTGCCATTTGCTTGAGCAGCTTTGAGACTAGAATCGCTTGTGAA

>IE_04H04_translated
MTLRSLILEMRSRPHRVVHDLAAAAAADSTSVSSQDYRWSEIPEELLREILIRVEAADGGGWPSRRSVVACAGVCRGWRLLMNETVVVPEISSKLTFPIS
LKQPGPRDSLVQCFIKRNRITQSYHLYLGLTNSLTDDGKFLLAACKLKHTTCTDYIISLRSDDMSRRSQAYVGKVRSNFLGTKFTVFDGNLLPSTGAAKL
RKSRSYNPAKVSAKVPLGSYPVAHITYELNVLGSRGPRKMQCLMDTIPTSTMEPQGVASEPSEFPLLGTRSTLSRSQSKPLRSSSSHLKETPLVLSNKTP
RWHEQLRCWCLNFHGRVTVASVKNFQLVAAGASCGSGTGMSPERQSERIILQFGKVGKDMFTMDYGYPISAFQAFAICLSSFETRIACE
Alignment ◆CDS（TAIRver.9）_without_stop_codonとのalignment◆

100%一致しました。

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