| Code name | IE_04H07 | |||
|---|---|---|---|---|
| Locus | AT5G14140 | |||
| Template | IT_04H07 (HR3763) | |||
| Donor Vector | pDONR207 | |||
| Forward primer | IPF_04H07 (ggggacaagtttgtacaaaaaagcaggcttcATGGCTACGGGTATGGCGGTAGATACGG) | |||
| Reverse primer | IPR_04H07 (ggggaccactttgtacaagaaagctgggttATTAGTTGGTCCTGGTGCAGACGAAGATTC) | |||
| Alignment with template seq. | 1st | アミノ酸配列100%一致 | 2nd | - |
| Alignment with TAIR7CDS | アミノ酸配列58.30%一致 | - | ||
| comment | 1st:Templateの配列とは一致 | |||
| sequence |
>IE_04H07_792bp ATGGCTACGGGTATGGCGGTAGATACGGTAGGCGAGTTAGGCTTTCCGTACTGGAACCCAATCCGACGAAGATTTCCTCCGGATTCGCCGTTCTTTGCAT CTGGAAATGTCGAGCGCGAGCTACTTGCTAAACAGGTGACATTGGACTTCACAGAAGATGAAATCAACCATCTGCATAAATTTGTGGAAATTGAAACCAG AAGAATCTCGTGTCCCATTGTTGGCTGTCCTGAACGCCTGAAATCTTTGGACCATTTTGAAGATCACTATAATGCACGGCACACTGCCTCTTGTTCAGTG TGCTCAAGGGTCTACCCTACTTCTCGCTTACTAAGTATTCACATATCCGAAGCACACGACTCTTTCTTTCAAGCTAAAGTTTCTCGGGGTTATGACATGT ATGAGTGCCTTGTGGAAGGGTGTGGATTGAAGTTCAAGACCTACAAAGCCCGGCATCGACACTTGATTGACAAACACAAGTTCCCAACGACATTTGAATT CTTCAAGAAGACCCAACTCTCAAAGAAGCGTAGAGAGAAACTTCAAAGGCAACATGTATCAAAGCTGAAACATGAAGAAGACAAAGAAGCTGCTTCGTCA GATGCCATGGAAGTAGAAGACAAAGGCAGCTTAGACGGTCTTGTTTCAGCACTGTCCTCGCTAACTACCTCAGACACAACTCCTTCGAATGTCAGCTTCG GTCGACGCCATGGTCGTGGGCTGACCTTTGTTCCACGGTCTGTTCATAGAGAGAAACGGACCGAATCTTCGTCTGCACCAGGACCAACTAAT >IE_04H07_translated MATGMAVDTVGELGFPYWNPIRRRFPPDSPFFASGNVERELLAKQVTLDFTEDEINHLHKFVEIETRRISCPIVGCPERLKSLDHFEDHYNARHTASCSV CSRVYPTSRLLSIHISEAHDSFFQAKVSRGYDMYECLVEGCGLKFKTYKARHRHLIDKHKFPTTFEFFKKTQLSKKRREKLQRQHVSKLKHEEDKEAASS DAMEVEDKGSLDGLVSALSSLTTSDTTPSNVSFGRRHGRGLTFVPRSVHREKRTESSSAPGPTN |
|||
| Alignment |
◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 777/1285 (60.5%)（アミノ酸配列で249/427 (58.3%)）一致しました。
10 20 30 40 50 60 70 80 90 100
AT5G14 ATGAGTTTTGTTAAGAAACTCGAGTTTAAAAATATAGTGGTGTTAACCAATGAGGTAGTGGTAAATCTCTCAAGTGAAGATATAAAAGAAAAAGAAAATG
IE_04H ----------------------------------------------------------------------------------------------------
110 120 130 140 150 160 170 180 190 200
AT5G14 GTGTTTCTTATACGTGGGCCTTCTTTGGGCCGAACAAGATATCATATAGTCCAGGAAGGATCCAGTTAATAAACCGGAGCGGAGGAAATGTTCAGTTGGA
IE_04H ----------------------------------------------------------------------------------------------------
210 220 230 240 250 260 270 280 290 300
AT5G14 TACTTGTTTCATGTTTGTGAGACCGATGGCTACGGGTATGGCGGTAGATACGGTAGGCGAGTTAGGCTTTCCGTACTGGAACCCAATCCGACGAAGATTT
:::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_04H -------------------------ATGGCTACGGGTATGGCGGTAGATACGGTAGGCGAGTTAGGCTTTCCGTACTGGAACCCAATCCGACGAAGATTT
10 20 30 40 50 60 70
310 320 330 340 350 360 370 380 390 400
AT5G14 CCTCCGGATTCGCCGTTCTTTGCATCTGGAAATGTCGAGCGCGAGCTACTTGCTAAACAGGTGACATTGGACTTCACAGAAGATGAAATCAACCATCTGC
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_04H CCTCCGGATTCGCCGTTCTTTGCATCTGGAAATGTCGAGCGCGAGCTACTTGCTAAACAGGTGACATTGGACTTCACAGAAGATGAAATCAACCATCTGC
80 90 100 110 120 130 140 150 160 170
410 420 430 440 450 460 470 480 490 500
AT5G14 ATAAATTTGTGGAAATTGAAACCAGAAGAATCTCGTGTCCCATTGTTGGCTGTCCTGAACGCCTGAAATCTTTGGACCATTTTGAAGATCACTATAATGC
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_04H ATAAATTTGTGGAAATTGAAACCAGAAGAATCTCGTGTCCCATTGTTGGCTGTCCTGAACGCCTGAAATCTTTGGACCATTTTGAAGATCACTATAATGC
180 190 200 210 220 230 240 250 260 270
510 520 530 540 550 560 570 580 590 600
AT5G14 ACGGCACACTGCCTCTTGTTCAGTGTGCTCAAGGGTCTACCCTACTTCTCGCTTACTAAGTATTCACATATCCGAAGCACACGACTCTTTCTTTCAAGCT
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_04H ACGGCACACTGCCTCTTGTTCAGTGTGCTCAAGGGTCTACCCTACTTCTCGCTTACTAAGTATTCACATATCCGAAGCACACGACTCTTTCTTTCAAGCT
280 290 300 310 320 330 340 350 360 370
610 620 630 640 650 660 670 680 690 700
AT5G14 AAAGTTTCTCGGGGTTATGACATGTATGAGTGCCTTGTGGAAGGGTGTGGATTGAAGTTCAAGACCTACAAAGCCCGGCATCGACACTTGATTGACAAAC
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_04H AAAGTTTCTCGGGGTTATGACATGTATGAGTGCCTTGTGGAAGGGTGTGGATTGAAGTTCAAGACCTACAAAGCCCGGCATCGACACTTGATTGACAAAC
380 390 400 410 420 430 440 450 460 470
710 720 730 740 750 760 770 780 790 800
AT5G14 ACAAGTTCCCAACGACATTTGAATTCTTCAAGAAGACCCAACTCTCAAAGAAGCGTAGAGAGAAACTTCAAAGGCAACATGTATCAAAGCTGAAACATGA
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_04H ACAAGTTCCCAACGACATTTGAATTCTTCAAGAAGACCCAACTCTCAAAGAAGCGTAGAGAGAAACTTCAAAGGCAACATGTATCAAAGCTGAAACATGA
480 490 500 510 520 530 540 550 560 570
810 820 830 840 850 860 870 880 890 900
AT5G14 AGAAGACAAAGAAGCTGCTTCGTCAGATGCCATGGAAGTAGAAGACAAAGGCAGCTTAGACGGTCTTGTTTCAGCACTGTCCTCGCTAACTACCTCAGAC
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_04H AGAAGACAAAGAAGCTGCTTCGTCAGATGCCATGGAAGTAGAAGACAAAGGCAGCTTAGACGGTCTTGTTTCAGCACTGTCCTCGCTAACTACCTCAGAC
580 590 600 610 620 630 640 650 660 670
910 920 930 940 950 960 970 980 990 1000
AT5G14 ACAACTCCTTCGAATGTCAGCTTCGGTCGACGCCATGGTCGTGGGCTGACCTTTGTTCCACGGATCCCTATTAATTCTAAAGTGGCTGCAGCTGTCGATG
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: :::: ::
IE_04H ACAACTCCTTCGAATGTCAGCTTCGGTCGACGCCATGGTCGTGGGCTGACCTTTGTTCCACGG---------------------------TCTGTTCATA
680 690 700 710 720 730 740
1010 1020 1030 1040 1050 1060 1070 1080 1090
AT5G14 GCGACGGCTGGATTTTGCCATCG--CCGAGATCAAGCAATGCGCTAGAGCTTCATGTTTTCCTTACAACATTACATCTACCTCTGCAGCCCCTTGATGAG
: :: :: : :: :::: :::: : :::::: : ::
IE_04H GAGA------GA-------AACGGACCGA---------------------------------------------ATCTTCGTCTGCACC---------AG
750 760 770 780
1100 1110 1120 1130 1140 1150 1160 1170 1180 1190
AT5G14 GAC--TCTTATGTTTGGAAACTTGTCGCTCAAGCGGTAATCTATGCTATTTGGAAGCAGAGAAACAACTTCATTCACAACCAGCAATCAATCCCTGCTGC
::: :: ::
IE_04H GACCAACTAAT-----------------------------------------------------------------------------------------
790
1200 1210 1220 1230 1240 1250 1260 1270 1280
AT5G14 TTGGGTGTTTAAGACCATTGACCGTGAGATGAGATCATTGACACCATCAATGCGAGGCGGCACCGGAAGAAGTTCAGAAACCTTA
IE_04H -------------------------------------------------------------------------------------
10 20 30 40 50 60 70 80 90 100
AT5G14 MSFVKKLEFKNIVVLTNEVVVNLSSEDIKEKENGVSYTWAFFGPNKISYSPGRIQLINRSGGNVQLDTCFMFVRPMATGMAVDTVGELGFPYWNPIRRRF
:::::::::::::::::::::::::
IE_04H ---------------------------------------------------------------------------MATGMAVDTVGELGFPYWNPIRRRF
10 20
110 120 130 140 150 160 170 180 190 200
AT5G14 PPDSPFFASGNVERELLAKQVTLDFTEDEINHLHKFVEIETRRISCPIVGCPERLKSLDHFEDHYNARHTASCSVCSRVYPTSRLLSIHISEAHDSFFQA
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_04H PPDSPFFASGNVERELLAKQVTLDFTEDEINHLHKFVEIETRRISCPIVGCPERLKSLDHFEDHYNARHTASCSVCSRVYPTSRLLSIHISEAHDSFFQA
30 40 50 60 70 80 90 100 110 120
210 220 230 240 250 260 270 280 290 300
AT5G14 KVSRGYDMYECLVEGCGLKFKTYKARHRHLIDKHKFPTTFEFFKKTQLSKKRREKLQRQHVSKLKHEEDKEAASSDAMEVEDKGSLDGLVSALSSLTTSD
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_04H KVSRGYDMYECLVEGCGLKFKTYKARHRHLIDKHKFPTTFEFFKKTQLSKKRREKLQRQHVSKLKHEEDKEAASSDAMEVEDKGSLDGLVSALSSLTTSD
130 140 150 160 170 180 190 200 210 220
310 320 330 340 350 360 370 380 390 400
AT5G14 TTPSNVSFGRRHGRGLTFVPRIPINSKVAAAVDGDGWILPSPRSSNALELHVFLTTLHLPLQPLDEDSYVWKLVAQAVIYAIWKQRNNFIHNQQSIPAAW
::::::::::::::::::::: : . : : .
IE_04H TTPSNVSFGRRHGRGLTFVPRSVHREKRTESSSA-----PGPTN--------------------------------------------------------
230 240 250 260
410 420
AT5G14 VFKTIDREMRSLTPSMRGGTGRSSETL
IE_04H ---------------------------
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