◆◆◆IE_04H10 detail◆◆◆

Code name	IE_04H10
Locus	AT1G10610
Template	IT_04H10 (HR3805)
Donor Vector	pDONR207
Forward primer	IPF_04H10 (ggggacaagtttgtacaaaaaagcaggcttcATGATGATGATGAGAGGTGGTGAGAGAG)
Reverse primer	IPR_04H10 (ggggaccactttgtacaagaaagctgggttCGTTACTACCTTGACATTGAGAACGGTCAT)
Alignment with template seq.	1st	アミノ酸配列100%一致	2nd	-
Alignment with TAIR7CDS	1st	アミノ酸配列94.80%一致	2nd	-
comment	1st:Templateの配列とは一致
sequence	>IE_04H10_1263bp ATGATGATGATGAGAGGTGGTGAGAGAGTGAAGGAGTTTCTTCGACCCTTTGTCGATTCCAGAACTTGGGACTTATGTGTTATCTGGAAACTCGGTGATG ATCCTTCTAGGTTTATTGAATGGGTGGGATGCTGCTGCAGTGGTTGTTATATTGATAAGAATATAAAGCTTGAAAACTCAGAAGAAGGAGGAACTGGGAG AAAAAAGAAGGCTTCTTTCTGTAGAGATGACCACAACAAGCATCGTATAAGAACCTTAGCTTGTGAAGCGCTTTCTCGTTTTCCTCTCTTCATGCCTCTC TATCCCGGGATTCATGGAGAAGTAGTGATGTCAAAATCTCCAAAGTGGTTGGTTAATTCAGGATCTAAAATGGAAATGTTCAGCACTCGTGTTCTTGTTC CTGTGAGTGATGGTCTCGTTGAGCTGTTCGCTTTCGATATGAGACCGTTTGATGAAAGCATGGTGCATTTGATCATGTCGCGTTGTACCACCTTCTTTGA ACCATTCCCTGAACAAAGGCTGCAGTTCAGGATCATTCCCCGAGCAGAGGAATCTATGAGTAGCGGTGTGAATCTCAGCGTTGAGGGCGGCGGATCATCA AGTGTTTCCAATCCCTCCAGTGAAACTCAGAATCTTTTCGGCAATTACCCGAATGCTAGTTGTGTGGAAATTCTCAGGGAGGAACAAACACCGTGTTTGA TAATGAACAAGGAAAAGGATGTTGTGGTGCAAAATGCCAACGATTCCAAAGCTAATAAGAAGCTGCTTCCTACGGAAAACTTCAAATCAAAGAACCTTCA TTCAGAGAGGAAAAGAAGAGAGAGAATTAATCAGGCCATGTATGGTCTAAGAGCCGTAGTCCCTAAAATCACAAAGTTGAACAAAATTGGAATTTTCAGT GATGCTGTTGATTACATCAATGAACTGCTAGTGGAGAAGCAGAAACTTGAAGATGAGCTTAAAGGAATCAATGAGATGGAATGCAAAGAAATCGCTGCAG AGGAACAATCTGCAATAGCTGATCCAGAAGCTGAAAGAGTTTCCTCTAAAAGCAACAAGAGAGTGAAGAAAAACGAGGTGAAAATTGAAGTCCACGAGAC TGGTGAGCGAGATTTCTTGATTCGGGTTGTGCAGGAACATAAACAAGATGGATTCAAGAGGTTGATAGAAGCTGTAGATTTATGTGAACTTGAGATCATT GACGTCAATTTCACCAGACTCGATCTCACAGTCATGACCGTTCTCAATGTCAAGGTAGTAACG >IE_04H10_translated MMMMRGGERVKEFLRPFVDSRTWDLCVIWKLGDDPSRFIEWVGCCCSGCYIDKNIKLENSEEGGTGRKKKASFCRDDHNKHRIRTLACEALSRFPLFMPL YPGIHGEVVMSKSPKWLVNSGSKMEMFSTRVLVPVSDGLVELFAFDMRPFDESMVHLIMSRCTTFFEPFPEQRLQFRIIPRAEESMSSGVNLSVEGGGSS SVSNPSSETQNLFGNYPNASCVEILREEQTPCLIMNKEKDVVVQNANDSKANKKLLPTENFKSKNLHSERKRRERINQAMYGLRAVVPKITKLNKIGIFS DAVDYINELLVEKQKLEDELKGINEMECKEIAAEEQSAIADPEAERVSSKSNKRVKKNEVKIEVHETGERDFLIRVVQEHKQDGFKRLIEAVDLCELEII DVNFTRLDLTVMTVLNVKVVT
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 1260/1325 (95.1%)（アミノ酸配列で418/441 (94.8%)）一致しました。 10 20 30 40 50 60 70 80 90 100 AT1G10 ATGATGATGATGAGAGGTGGTGAGAGAGTGAAGGAGTTTCTTCGACCCTTTGTCGATTCCAGAACTTGGGACTTATGTGTTATCTGGAAACTCGGTGATG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04H ATGATGATGATGAGAGGTGGTGAGAGAGTGAAGGAGTTTCTTCGACCCTTTGTCGATTCCAGAACTTGGGACTTATGTGTTATCTGGAAACTCGGTGATG 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT1G10 ATCCTTCTAGGTTTATTGAATGGGTGGGATGCTGCTGCAGTGGTTGTTATATTGATAAGAATATAAAGCTTGAAAACTCAGAAGAAGGAGGAACTGGGAG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04H ATCCTTCTAGGTTTATTGAATGGGTGGGATGCTGCTGCAGTGGTTGTTATATTGATAAGAATATAAAGCTTGAAAACTCAGAAGAAGGAGGAACTGGGAG 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT1G10 AAAAAAGAAGGCTTCTTTCTGTAGAGATGACCACAACAAGCATCGTATAAGAACCTTAGCTTGTGAAGCGCTTTCTCGTTTTCCTCTCTTCATGCCTCTC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04H AAAAAAGAAGGCTTCTTTCTGTAGAGATGACCACAACAAGCATCGTATAAGAACCTTAGCTTGTGAAGCGCTTTCTCGTTTTCCTCTCTTCATGCCTCTC 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 AT1G10 TATCCCGGGATTCATGGAGAAGTAGTGATGTCAAAATCTCCAAAGTGGTTGGTTAATTCAGGATCTAAAATGGAAATGTTCAGCACTCGTGTTCTTGTTC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04H TATCCCGGGATTCATGGAGAAGTAGTGATGTCAAAATCTCCAAAGTGGTTGGTTAATTCAGGATCTAAAATGGAAATGTTCAGCACTCGTGTTCTTGTTC 310 320 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 480 490 500 AT1G10 CTGTGAGTGATGGTCTCGTTGAGCTGTTCGCTTTCGATATGAGACCGTTTGATGAAAGCATGGTGCATTTGATCATGTCGCGTTGTACCACCTTCTTTGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04H CTGTGAGTGATGGTCTCGTTGAGCTGTTCGCTTTCGATATGAGACCGTTTGATGAAAGCATGGTGCATTTGATCATGTCGCGTTGTACCACCTTCTTTGA 410 420 430 440 450 460 470 480 490 500 510 520 530 540 550 560 570 580 590 600 AT1G10 ACCATTCCCTGAACAAAGGCTGCAGTTCAGGATCATTCCCCGAGCAGAGGAATCTATGAGTAGCGGTGTGAATCTCAGCGTTGAGGGCGGCGGATCATCA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04H ACCATTCCCTGAACAAAGGCTGCAGTTCAGGATCATTCCCCGAGCAGAGGAATCTATGAGTAGCGGTGTGAATCTCAGCGTTGAGGGCGGCGGATCATCA 510 520 530 540 550 560 570 580 590 600 610 620 630 640 650 660 670 680 690 700 AT1G10 AGTGTTTCCAATCCCTCCAGTGAAACTCAGAATCTTTTCGGCAATTACCCGAATGCTAGTTGTGTGGAAATTCTCAGGGAGGAACAAACACCGTGTTTGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04H AGTGTTTCCAATCCCTCCAGTGAAACTCAGAATCTTTTCGGCAATTACCCGAATGCTAGTTGTGTGGAAATTCTCAGGGAGGAACAAACACCGTGTTTGA 610 620 630 640 650 660 670 680 690 700 710 720 730 740 750 760 770 780 790 800 AT1G10 TAATGAACAAGGAAAAGGATGTTGTGGTGCAAAATGCCAACGATTCCAAAGCTAATAAGAAGCTGCTTCCTACGGAAAACTTCAAATCAAAGAACCTTCA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04H TAATGAACAAGGAAAAGGATGTTGTGGTGCAAAATGCCAACGATTCCAAAGCTAATAAGAAGCTGCTTCCTACGGAAAACTTCAAATCAAAGAACCTTCA 710 720 730 740 750 760 770 780 790 800 810 820 830 840 850 860 870 880 890 900 AT1G10 TTCAGAGAGGAAAAGAAGAGAGAGAATTAATCAGGCCATGTATGGTCTAAGAGCCGTAGTCCCTAAAATCACAAAGTTGAACAAAATTGGAATTTTCAGT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04H TTCAGAGAGGAAAAGAAGAGAGAGAATTAATCAGGCCATGTATGGTCTAAGAGCCGTAGTCCCTAAAATCACAAAGTTGAACAAAATTGGAATTTTCAGT 810 820 830 840 850 860 870 880 890 900 910 920 930 940 950 960 970 980 990 1000 AT1G10 GATGCTGTTGATTACATCAATGAACTGCTAGTGGAGAAGCAGAAACTTGAAGATGAGCTTAAAGGAATCAATGAGATGGAATGCAAAGAAATCGCTGCAG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04H GATGCTGTTGATTACATCAATGAACTGCTAGTGGAGAAGCAGAAACTTGAAGATGAGCTTAAAGGAATCAATGAGATGGAATGCAAAGAAATCGCTGCAG 910 920 930 940 950 960 970 980 990 1000 1010 1020 1030 1040 1050 1060 1070 1080 1090 1100 AT1G10 AGGAACAATCTGCAATAGCTGATCCAGAAGCTGAAAGAGTTTCCTCTAAAAGCAACAAGAGAGTGAAGAAAAACGAGGTGAAAATTGAAGTCCACGAGAC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04H AGGAACAATCTGCAATAGCTGATCCAGAAGCTGAAAGAGTTTCCTCTAAAAGCAACAAGAGAGTGAAGAAAAACGAGGTGAAAATTGAAGTCCACGAGAC 1010 1020 1030 1040 1050 1060 1070 1080 1090 1100 1110 1120 1130 1140 1150 1160 1170 1180 1190 1200 AT1G10 TGGTGAGCGAGATTTCTTGATTCGGGTTGTGCAGGAACATAAACAAGATGGATTCAAGAGGTTGATAGAAGCTGTAGATTTATGTGAACTTGAGATCATT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04H TGGTGAGCGAGATTTCTTGATTCGGGTTGTGCAGGAACATAAACAAGATGGATTCAAGAGGTTGATAGAAGCTGTAGATTTATGTGAACTTGAGATCATT 1110 1120 1130 1140 1150 1160 1170 1180 1190 1200 1210 1220 1230 1240 1250 1260 1270 1280 1290 AT1G10 GACGTCAATTTCACCAGACTCGATCTCACAGTCATGACCGTTCTCAATGTCAAGGCGAACAAAG--ACGGGATTGCATGTGGAATATTGAGAGATTTACT ::::::::::::::::::::::::::::::::::::::::::::::::::::::: :: ::: IE_04H GACGTCAATTTCACCAGACTCGATCTCACAGTCATGACCGTTCTCAATGTCAAGG------TAGTAACG------------------------------- 1210 1220 1230 1240 1250 1260 1300 1310 1320 AT1G10 GCTCAAGATGATGATAACTTCAATA IE_04H ------------------------- 10 20 30 40 50 60 70 80 90 100 AT1G10 MMMMRGGERVKEFLRPFVDSRTWDLCVIWKLGDDPSRFIEWVGCCCSGCYIDKNIKLENSEEGGTGRKKKASFCRDDHNKHRIRTLACEALSRFPLFMPL :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04H MMMMRGGERVKEFLRPFVDSRTWDLCVIWKLGDDPSRFIEWVGCCCSGCYIDKNIKLENSEEGGTGRKKKASFCRDDHNKHRIRTLACEALSRFPLFMPL 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT1G10 YPGIHGEVVMSKSPKWLVNSGSKMEMFSTRVLVPVSDGLVELFAFDMRPFDESMVHLIMSRCTTFFEPFPEQRLQFRIIPRAEESMSSGVNLSVEGGGSS :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04H YPGIHGEVVMSKSPKWLVNSGSKMEMFSTRVLVPVSDGLVELFAFDMRPFDESMVHLIMSRCTTFFEPFPEQRLQFRIIPRAEESMSSGVNLSVEGGGSS 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT1G10 SVSNPSSETQNLFGNYPNASCVEILREEQTPCLIMNKEKDVVVQNANDSKANKKLLPTENFKSKNLHSERKRRERINQAMYGLRAVVPKITKLNKIGIFS :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04H SVSNPSSETQNLFGNYPNASCVEILREEQTPCLIMNKEKDVVVQNANDSKANKKLLPTENFKSKNLHSERKRRERINQAMYGLRAVVPKITKLNKIGIFS 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 AT1G10 DAVDYINELLVEKQKLEDELKGINEMECKEIAAEEQSAIADPEAERVSSKSNKRVKKNEVKIEVHETGERDFLIRVVQEHKQDGFKRLIEAVDLCELEII :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_04H DAVDYINELLVEKQKLEDELKGINEMECKEIAAEEQSAIADPEAERVSSKSNKRVKKNEVKIEVHETGERDFLIRVVQEHKQDGFKRLIEAVDLCELEII 310 320 330 340 350 360 370 380 390 400 410 420 430 440 AT1G10 DVNFTRLDLTVMTVLNVKANKDGIACGILRDLLLKMMITSI :::::::::::::::::: IE_04H DVNFTRLDLTVMTVLNVKVVT-------------------- 410 420

このページのTOPに戻る