◆◆◆IE_05A05 detail◆◆◆

Code name	IE_05A05
Locus	AT5G18830
Template	IT_05A05 (HR0299)
Donor Vector	pDONR207
Forward primer	IPF_05A05 (ggggacaagtttgtacaaaaaagcaggcttcATGTCTTCTCTGTCGCAATCGCCACCAC)
Reverse primer	IPR_05A05 (ggggaccactttgtacaagaaagctgggttAATTTTGTGTACCAATCTCATTCGGATTGC)
Alignment with template seq.	1st	アミノ酸配列100%一致	2nd	-
Alignment with TAIR7CDS	1st	アミノ酸配列96.80%一致	2nd	-
comment	1st:Templateの配列とは一致
sequence	>IE_05A05_2325bp ATGTCTTCTCTGTCGCAATCGCCACCACCGCCGGAGATGGATATCCAACCCCCGGCATTGGTTAACGATGATCCTTCCACTTATTCCTCCGCTTTATGGG ATTGGGGAGATCTCCTTGACTTCGCCGCAGACGAACGCCTTCTCGTCGATCAAATTCATTTCCCTCCCGTTCTCTCTCCTCCTCTACCGCCTCTGATTCC GACGCAAACTCCGGCGGAATCTGAATTAGATCCTTCTCCGGAAGAATCGGGTTCTGGTTCGGATCGGGTTAGGAAGCGAGACCCGAGGTTGATTTGTTCC AATTTCATTGAAGGTATGCTTCCTTGTTCGTGTCCTGAGCTTGATCAGAAATTGGAAGACGCCGAGCTTCCGAAGAAGAAACGGGTTCGCGGCGGGTCGG GCGTGGCTCGATGTCAGGTTCCGGATTGTGAAGCGGATATTAGCGAGCTCAAAGGGTACCATAAGAGGCATAGGGTTTGTCTCCGTTGCGCTACCGCCAG CTTTGTTGTGCTTGATGGAGAGAATAAGAGATACTGTCAACAGTGTGGAAAGTTTCATTTGCTCCCGGACTTTGATGAAGGAAAACGCAGCTGTCGGAGA AAGCTAGAGCGTCACAACAACAGACGGAAAAGGAAACCTGTAGATAAAGGAGGTGTTGCTGCAGAACAACAGCAAGTGCTTTCACAGAATGATAACAGCG TCATTGATGTTGAGGATGGAAAAGATATCACATGCTCTAGTGACCAGAGAGCTGAAGAAGAGCCTTCATTGATTTTTGAAGATCGGCATATTACCACTCA GGGTTCTGTACCTTTTACCCGGAGCATCAACGCAGACAACTTTGTCTCTGTTACAGGTTCGGGTGAAGCTCAACCAGATGAAGGAATGAATGACACAAAA TTTGAACGTTCACCTTCTAATGGCGACAACAAAAGTGCTTACTCAACTGTGTGTCCAACTGGTCGGATCTCTTTTAAGCTCTACGATTGGAATCCAGCGG AGTTCCCACGGAGACTACGTCATCAAATATTTCAATGGTTGGCCAACATGCCTGTTGAGCTGGAGGGCTATATCCGCCCAGGATGTACAATTTTGACTGT CTTTATAGCAATGCCAGAGATTATGTGGGCGAAGTTATCTAAAGATCCTGTGGCATATCTGGATGAATTTATTCTTAAACCTGGAAAGATGCTGTTTGGA AGAGGCTCGATGACTGTCTATTTGAACAACATGATTTTCCGTCTTATTAAAGGTGGAACAACTTTAAAGAGAGTCGATGTAAAATTAGAGTCGCCGAAAC TTCAGTTTGTGTATCCTACATGTTTTGAAGCTGGAAAACCAATTGAACTCGTTGTATGTGGACAAAACCTTCTGCAACCTAAATGCCGGTTTCTCGTGTC TTTTTCTGGGAAGTACTTGCCACATAACTATTCTGTTGTACCTGCACCGGACCAGGATGGGAAGCGTTCTTGTAATAACAAGTTCTACAAGATCAATATT GTGAATTCTGACCCTAGTCTCTTTGGCCCTGCCTTTGTCGAGGTTGAAAATGAATCTGGCCTATCAAATTTCATACCTCTAATTATTGGAGATGCAGCTG TTTGTTCCGAAATGAAACTAATAGAGCAGAAGTTCAATGCTACACTCTTTCCAGAGGGACAAGAAGTTACTGCTTGCTCTTCTTTGACTTGCTGTTGCAG GGATTTTGGGGAGAGACAGAGCACCTTTAGTGGCCTCTTGTTAGATATTGCATGGTCGGTGAAGGTACCCTCTGCAGAACGCACTGAGCAACCTGTGAAC CGATGTCAGATAAAAAGATACAACAGAGTGTTGAATTATCTGATACAGAATAACTCAGCGTCGATCTTAGGAAACGTACTGCACAATTTGGAAACTTTGG TGAAGAAAATGGAGCCAGACAGTCTTGTTCACTGTACCTGTGACTGCGACGTGAGGCTTCTACATGAAAATATGGATTTGGCGAGTGATATTCACAGAAA GCATCAAAGCCCTATAGAGTCAAAGGATCCTGAAGCAGGATTAGATTGTAAAGAGAGAATACAGGCAGACTGTTCACCAGATAGCGGCGGAAAAGAGACT GATCCTCTGTTAAACAAAGAGGTTGTCATGAACGTAAATGACATAGGAGACTGGCCAAGGAAGTCGTGTATAAAAACGCACTCAGCTTTAGCATTCAGGT CCCGTCAAACTATGTTCTTAATCGCTACGTTCGCTGTCTGTTTTGCTGTCTGTGCGGTTCTCTACCATCCAAACAAGGTCACACAGCTTGCAGTGGCAAT CCGAATGAGATTGGTACACAAAATT >IE_05A05_translated MSSLSQSPPPPEMDIQPPALVNDDPSTYSSALWDWGDLLDFAADERLLVDQIHFPPVLSPPLPPLIPTQTPAESELDPSPEESGSGSDRVRKRDPRLICS NFIEGMLPCSCPELDQKLEDAELPKKKRVRGGSGVARCQVPDCEADISELKGYHKRHRVCLRCATASFVVLDGENKRYCQQCGKFHLLPDFDEGKRSCRR KLERHNNRRKRKPVDKGGVAAEQQQVLSQNDNSVIDVEDGKDITCSSDQRAEEEPSLIFEDRHITTQGSVPFTRSINADNFVSVTGSGEAQPDEGMNDTK FERSPSNGDNKSAYSTVCPTGRISFKLYDWNPAEFPRRLRHQIFQWLANMPVELEGYIRPGCTILTVFIAMPEIMWAKLSKDPVAYLDEFILKPGKMLFG RGSMTVYLNNMIFRLIKGGTTLKRVDVKLESPKLQFVYPTCFEAGKPIELVVCGQNLLQPKCRFLVSFSGKYLPHNYSVVPAPDQDGKRSCNNKFYKINI VNSDPSLFGPAFVEVENESGLSNFIPLIIGDAAVCSEMKLIEQKFNATLFPEGQEVTACSSLTCCCRDFGERQSTFSGLLLDIAWSVKVPSAERTEQPVN RCQIKRYNRVLNYLIQNNSASILGNVLHNLETLVKKMEPDSLVHCTCDCDVRLLHENMDLASDIHRKHQSPIESKDPEAGLDCKERIQADCSPDSGGKET DPLLNKEVVMNVNDIGDWPRKSCIKTHSALAFRSRQTMFLIATFAVCFAVCAVLYHPNKVTQLAVAIRMRLVHKI
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 2324/2403 (96.7%)（アミノ酸配列で775/801 (96.8%)）一致しました。 10 20 30 40 50 60 70 80 90 100 AT5G18 ATGTCTTCTCTGTCGCAATCGCCACCACCGCCGGAGATGGATATCCAACCCCCGGCATTGGTTAACGATGATCCTTCCACTTATTCCTCCGCTTTATGGG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05A ATGTCTTCTCTGTCGCAATCGCCACCACCGCCGGAGATGGATATCCAACCCCCGGCATTGGTTAACGATGATCCTTCCACTTATTCCTCCGCTTTATGGG 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT5G18 ATTGGGGAGATCTCCTTGACTTCGCCGCAGACGAACGCCTTCTCGTCGATCAAATTCATTTCCCTCCCGTTCTCTCTCCTCCTCTACCGCCTCTGATTCC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05A ATTGGGGAGATCTCCTTGACTTCGCCGCAGACGAACGCCTTCTCGTCGATCAAATTCATTTCCCTCCCGTTCTCTCTCCTCCTCTACCGCCTCTGATTCC 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT5G18 GACGCAAACTCCGGCGGAATCTGAATTAGATCCTTCTCCGGAAGAATCGGGTTCTGGTTCGGATCGGGTTAGGAAGCGAGACCCGAGGTTGATTTGTTCC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05A GACGCAAACTCCGGCGGAATCTGAATTAGATCCTTCTCCGGAAGAATCGGGTTCTGGTTCGGATCGGGTTAGGAAGCGAGACCCGAGGTTGATTTGTTCC 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 AT5G18 AATTTCATTGAAGGTATGCTTCCTTGTTCGTGTCCTGAGCTTGATCAGAAATTGGAAGACGCCGAGCTTCCGAAGAAGAAACGGGTTCGCGGCGGGTCGG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05A AATTTCATTGAAGGTATGCTTCCTTGTTCGTGTCCTGAGCTTGATCAGAAATTGGAAGACGCCGAGCTTCCGAAGAAGAAACGGGTTCGCGGCGGGTCGG 310 320 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 480 490 500 AT5G18 GCGTGGCTCGATGTCAGGTTCCGGATTGTGAAGCGGATATTAGCGAGCTCAAAGGGTACCATAAGAGGCATAGGGTTTGTCTCCGTTGCGCTACCGCCAG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05A GCGTGGCTCGATGTCAGGTTCCGGATTGTGAAGCGGATATTAGCGAGCTCAAAGGGTACCATAAGAGGCATAGGGTTTGTCTCCGTTGCGCTACCGCCAG 410 420 430 440 450 460 470 480 490 500 510 520 530 540 550 560 570 580 590 600 AT5G18 CTTTGTTGTGCTTGATGGAGAGAATAAGAGATACTGTCAACAGTGTGGAAAGTTTCATTTGCTCCCGGACTTTGATGAAGGAAAACGCAGCTGTCGGAGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05A CTTTGTTGTGCTTGATGGAGAGAATAAGAGATACTGTCAACAGTGTGGAAAGTTTCATTTGCTCCCGGACTTTGATGAAGGAAAACGCAGCTGTCGGAGA 510 520 530 540 550 560 570 580 590 600 610 620 630 640 650 660 670 680 690 700 AT5G18 AAGCTAGAGCGTCACAACAACAGACGGAAAAGGAAACCTGTAGATAAAGGAGGTGTTGCTGCAGAACAACAGCAAGTGCTTTCACAGAATGATAACAGCG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05A AAGCTAGAGCGTCACAACAACAGACGGAAAAGGAAACCTGTAGATAAAGGAGGTGTTGCTGCAGAACAACAGCAAGTGCTTTCACAGAATGATAACAGCG 610 620 630 640 650 660 670 680 690 700 710 720 730 740 750 760 770 780 790 800 AT5G18 TCATTGATGTTGAGGATGGAAAAGATATCACATGCTCTAGTGACCAGAGAGCTGAAGAAGAGCCTTCATTGATTTTTGAAGATCGGCATATTACCACTCA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05A TCATTGATGTTGAGGATGGAAAAGATATCACATGCTCTAGTGACCAGAGAGCTGAAGAAGAGCCTTCATTGATTTTTGAAGATCGGCATATTACCACTCA 710 720 730 740 750 760 770 780 790 800 810 820 830 840 850 860 870 880 890 900 AT5G18 GGGTTCTGTACCTTTTACCCGAAGCATCAACGCAGACAACTTTGTCTCTGTTACAGGTTCGGGTGAAGCTCAACCAGATGAAGGAATGAATGACACAAAA ::::::::::::::::::::: :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05A GGGTTCTGTACCTTTTACCCGGAGCATCAACGCAGACAACTTTGTCTCTGTTACAGGTTCGGGTGAAGCTCAACCAGATGAAGGAATGAATGACACAAAA 810 820 830 840 850 860 870 880 890 900 910 920 930 940 950 960 970 980 990 1000 AT5G18 TTTGAACGTTCACCTTCTAATGGCGACAACAAAAGTGCTTACTCAACTGTGTGTCCAACTGGTCGGATCTCTTTTAAGCTCTACGATTGGAATCCAGCGG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05A TTTGAACGTTCACCTTCTAATGGCGACAACAAAAGTGCTTACTCAACTGTGTGTCCAACTGGTCGGATCTCTTTTAAGCTCTACGATTGGAATCCAGCGG 910 920 930 940 950 960 970 980 990 1000 1010 1020 1030 1040 1050 1060 1070 1080 1090 1100 AT5G18 AGTTCCCACGGAGACTACGTCATCAAATATTTCAATGGTTGGCCAACATGCCTGTTGAGCTGGAGGGCTATATCCGCCCAGGATGTACAATTTTGACTGT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05A AGTTCCCACGGAGACTACGTCATCAAATATTTCAATGGTTGGCCAACATGCCTGTTGAGCTGGAGGGCTATATCCGCCCAGGATGTACAATTTTGACTGT 1010 1020 1030 1040 1050 1060 1070 1080 1090 1100 1110 1120 1130 1140 1150 1160 1170 1180 1190 1200 AT5G18 CTTTATAGCAATGCCAGAGATTATGTGGGCGAAGTTATCTAAAGATCCTGTGGCATATCTGGATGAATTTATTCTTAAACCTGGAAAGATGCTGTTTGGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05A CTTTATAGCAATGCCAGAGATTATGTGGGCGAAGTTATCTAAAGATCCTGTGGCATATCTGGATGAATTTATTCTTAAACCTGGAAAGATGCTGTTTGGA 1110 1120 1130 1140 1150 1160 1170 1180 1190 1200 1210 1220 1230 1240 1250 1260 1270 1280 1290 1300 AT5G18 AGAGGCTCGATGACTGTCTATTTGAACAACATGATTTTCCGTCTTATTAAAGGTGGAACAACTTTAAAGAGAGTCGATGTAAAATTAGAGTCGCCGAAAC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05A AGAGGCTCGATGACTGTCTATTTGAACAACATGATTTTCCGTCTTATTAAAGGTGGAACAACTTTAAAGAGAGTCGATGTAAAATTAGAGTCGCCGAAAC 1210 1220 1230 1240 1250 1260 1270 1280 1290 1300 1310 1320 1330 1340 1350 1360 1370 1380 1390 1400 AT5G18 TTCAGTTTGTGTATCCTACATGTTTTGAAGCTGGAAAACCAATTGAACTCGTTGTATGTGGACAAAACCTTCTGCAACCTAAATGCCGGTTTCTCGTGTC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05A TTCAGTTTGTGTATCCTACATGTTTTGAAGCTGGAAAACCAATTGAACTCGTTGTATGTGGACAAAACCTTCTGCAACCTAAATGCCGGTTTCTCGTGTC 1310 1320 1330 1340 1350 1360 1370 1380 1390 1400 1410 1420 1430 1440 1450 1460 1470 1480 1490 1500 AT5G18 TTTTTCTGGGAAGTACTTGCCACATAACTATTCTGTTGTACCTGCACCGGACCAGGATGGGAAGCGTTCTTGTAATAACAAGTTCTACAAGATCAATATT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05A TTTTTCTGGGAAGTACTTGCCACATAACTATTCTGTTGTACCTGCACCGGACCAGGATGGGAAGCGTTCTTGTAATAACAAGTTCTACAAGATCAATATT 1410 1420 1430 1440 1450 1460 1470 1480 1490 1500 1510 1520 1530 1540 1550 1560 1570 1580 1590 1600 AT5G18 GTGAATTCTGACCCTAGTCTCTTTGGCCCTGCCTTTGTCGAGGTTGAAAATGAATCTGGCCTATCAAATTTCATACCTCTAATTATTGGAGATGCAGCTG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05A GTGAATTCTGACCCTAGTCTCTTTGGCCCTGCCTTTGTCGAGGTTGAAAATGAATCTGGCCTATCAAATTTCATACCTCTAATTATTGGAGATGCAGCTG 1510 1520 1530 1540 1550 1560 1570 1580 1590 1600 1610 1620 1630 1640 1650 1660 1670 1680 1690 1700 AT5G18 TTTGTTCCGAAATGAAACTAATAGAGCAGAAGTTCAATGCTACACTCTTTCCAGAGGGACAAGAAGTTACTGCTTGCTCTTCTTTGACTTGCTGTTGCAG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05A TTTGTTCCGAAATGAAACTAATAGAGCAGAAGTTCAATGCTACACTCTTTCCAGAGGGACAAGAAGTTACTGCTTGCTCTTCTTTGACTTGCTGTTGCAG 1610 1620 1630 1640 1650 1660 1670 1680 1690 1700 1710 1720 1730 1740 1750 1760 1770 1780 1790 1800 AT5G18 GGATTTTGGGGAGAGACAGAGCACCTTTAGTGGCCTCTTGTTAGATATTGCATGGTCGGTGAAGGTACCCTCTGCAGAACGCACTGAGCAACCTGTGAAC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05A GGATTTTGGGGAGAGACAGAGCACCTTTAGTGGCCTCTTGTTAGATATTGCATGGTCGGTGAAGGTACCCTCTGCAGAACGCACTGAGCAACCTGTGAAC 1710 1720 1730 1740 1750 1760 1770 1780 1790 1800 1810 1820 1830 1840 1850 1860 1870 1880 1890 1900 AT5G18 CGATGTCAGATAAAAAGATACAACAGAGTGTTGAATTATCTGATACAGAATAACTCAGCGTCGATCTTAGGAAACGTACTGCACAATTTGGAAACTTTGG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05A CGATGTCAGATAAAAAGATACAACAGAGTGTTGAATTATCTGATACAGAATAACTCAGCGTCGATCTTAGGAAACGTACTGCACAATTTGGAAACTTTGG 1810 1820 1830 1840 1850 1860 1870 1880 1890 1900 1910 1920 1930 1940 1950 1960 1970 1980 1990 2000 AT5G18 TGAAGAAAATGGAGCCAGACAGTCTTGTTCACTGTACCTGTGACTGCGACGTGAGGCTTCTACATGAAAATATGGATTTGGCGAGTGATATTCACAGAAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05A TGAAGAAAATGGAGCCAGACAGTCTTGTTCACTGTACCTGTGACTGCGACGTGAGGCTTCTACATGAAAATATGGATTTGGCGAGTGATATTCACAGAAA 1910 1920 1930 1940 1950 1960 1970 1980 1990 2000 2010 2020 2030 2040 2050 2060 2070 2080 2090 2100 AT5G18 GCATCAAAGCCCTATAGAGTCAAAGGTGAATCCTCCTTCGTCAGGTTGCTGTTGTGTGAGTAGTCAGAAGGACATACCATCAAGAATATTAAACTTCAAT :::::::::::::::::::::: IE_05A GCATCAAAGCCCTATAGAGTCA------------------------------------------------------------------------------ 2010 2020 2110 2120 2130 2140 2150 2160 2170 2180 2190 2200 AT5G18 AAGGATCCTGAAGCAGGATTAGATTGTAAAGAGAGAATACAGGCAGACTGTTCACCAGATAGCGGCGGAAAAGAGACTGATCCTCTGTTAAACAAAGAGG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05A AAGGATCCTGAAGCAGGATTAGATTGTAAAGAGAGAATACAGGCAGACTGTTCACCAGATAGCGGCGGAAAAGAGACTGATCCTCTGTTAAACAAAGAGG 2030 2040 2050 2060 2070 2080 2090 2100 2110 2120 2210 2220 2230 2240 2250 2260 2270 2280 2290 2300 AT5G18 TTGTCATGAACGTAAATGACATAGGAGACTGGCCAAGGAAGTCGTGTATAAAAACGCACTCAGCTTTAGCATTCAGGTCCCGTCAAACTATGTTCTTAAT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05A TTGTCATGAACGTAAATGACATAGGAGACTGGCCAAGGAAGTCGTGTATAAAAACGCACTCAGCTTTAGCATTCAGGTCCCGTCAAACTATGTTCTTAAT 2130 2140 2150 2160 2170 2180 2190 2200 2210 2220 2310 2320 2330 2340 2350 2360 2370 2380 2390 2400 AT5G18 CGCTACGTTCGCTGTCTGTTTTGCTGTCTGTGCGGTTCTCTACCATCCAAACAAGGTCACACAGCTTGCAGTGGCAATCCGAATGAGATTGGTACACAAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05A CGCTACGTTCGCTGTCTGTTTTGCTGTCTGTGCGGTTCTCTACCATCCAAACAAGGTCACACAGCTTGCAGTGGCAATCCGAATGAGATTGGTACACAAA 2230 2240 2250 2260 2270 2280 2290 2300 2310 2320 AT5G18 ATT ::: IE_05A ATT 10 20 30 40 50 60 70 80 90 100 AT5G18 MSSLSQSPPPPEMDIQPPALVNDDPSTYSSALWDWGDLLDFAADERLLVDQIHFPPVLSPPLPPLIPTQTPAESELDPSPEESGSGSDRVRKRDPRLICS :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05A MSSLSQSPPPPEMDIQPPALVNDDPSTYSSALWDWGDLLDFAADERLLVDQIHFPPVLSPPLPPLIPTQTPAESELDPSPEESGSGSDRVRKRDPRLICS 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT5G18 NFIEGMLPCSCPELDQKLEDAELPKKKRVRGGSGVARCQVPDCEADISELKGYHKRHRVCLRCATASFVVLDGENKRYCQQCGKFHLLPDFDEGKRSCRR :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05A NFIEGMLPCSCPELDQKLEDAELPKKKRVRGGSGVARCQVPDCEADISELKGYHKRHRVCLRCATASFVVLDGENKRYCQQCGKFHLLPDFDEGKRSCRR 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT5G18 KLERHNNRRKRKPVDKGGVAAEQQQVLSQNDNSVIDVEDGKDITCSSDQRAEEEPSLIFEDRHITTQGSVPFTRSINADNFVSVTGSGEAQPDEGMNDTK :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05A KLERHNNRRKRKPVDKGGVAAEQQQVLSQNDNSVIDVEDGKDITCSSDQRAEEEPSLIFEDRHITTQGSVPFTRSINADNFVSVTGSGEAQPDEGMNDTK 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 AT5G18 FERSPSNGDNKSAYSTVCPTGRISFKLYDWNPAEFPRRLRHQIFQWLANMPVELEGYIRPGCTILTVFIAMPEIMWAKLSKDPVAYLDEFILKPGKMLFG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05A FERSPSNGDNKSAYSTVCPTGRISFKLYDWNPAEFPRRLRHQIFQWLANMPVELEGYIRPGCTILTVFIAMPEIMWAKLSKDPVAYLDEFILKPGKMLFG 310 320 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 480 490 500 AT5G18 RGSMTVYLNNMIFRLIKGGTTLKRVDVKLESPKLQFVYPTCFEAGKPIELVVCGQNLLQPKCRFLVSFSGKYLPHNYSVVPAPDQDGKRSCNNKFYKINI :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05A RGSMTVYLNNMIFRLIKGGTTLKRVDVKLESPKLQFVYPTCFEAGKPIELVVCGQNLLQPKCRFLVSFSGKYLPHNYSVVPAPDQDGKRSCNNKFYKINI 410 420 430 440 450 460 470 480 490 500 510 520 530 540 550 560 570 580 590 600 AT5G18 VNSDPSLFGPAFVEVENESGLSNFIPLIIGDAAVCSEMKLIEQKFNATLFPEGQEVTACSSLTCCCRDFGERQSTFSGLLLDIAWSVKVPSAERTEQPVN :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05A VNSDPSLFGPAFVEVENESGLSNFIPLIIGDAAVCSEMKLIEQKFNATLFPEGQEVTACSSLTCCCRDFGERQSTFSGLLLDIAWSVKVPSAERTEQPVN 510 520 530 540 550 560 570 580 590 600 610 620 630 640 650 660 670 680 690 700 AT5G18 RCQIKRYNRVLNYLIQNNSASILGNVLHNLETLVKKMEPDSLVHCTCDCDVRLLHENMDLASDIHRKHQSPIESKVNPPSSGCCCVSSQKDIPSRILNFN :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05A RCQIKRYNRVLNYLIQNNSASILGNVLHNLETLVKKMEPDSLVHCTCDCDVRLLHENMDLASDIHRKHQSPIES-------------------------- 610 620 630 640 650 660 670 710 720 730 740 750 760 770 780 790 800 AT5G18 KDPEAGLDCKERIQADCSPDSGGKETDPLLNKEVVMNVNDIGDWPRKSCIKTHSALAFRSRQTMFLIATFAVCFAVCAVLYHPNKVTQLAVAIRMRLVHK :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05A KDPEAGLDCKERIQADCSPDSGGKETDPLLNKEVVMNVNDIGDWPRKSCIKTHSALAFRSRQTMFLIATFAVCFAVCAVLYHPNKVTQLAVAIRMRLVHK 680 690 700 710 720 730 740 750 760 770 AT5G18 I : IE_05A I

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