◆◆◆IE_05A11 detail◆◆◆

Code name	IE_05A11
Locus	AT1G09710
Template	IT_05A11 (HR0636)
Donor Vector	pDONR207
Forward primer	IPF_05A11 (ggggacaagtttgtacaaaaaagcaggcttcATGGTTGCTAATAATAATACTAGTAGCA)
Reverse primer	IPR_05A11 (ggggaccactttgtacaagaaagctgggttAACACCCTGTCCGGTCCCTGCCACAGTTGC)
Alignment with template seq.	1st	アミノ酸配列100%一致	2nd	-
Alignment with TAIR7CDS	1st	アミノ酸配列99.80%一致	2nd	-
comment	1st:Templateの配列とは一致
sequence	>IE_05A11_1830bp ATGGTTGCTAATAATAATACTAGTAGCAATCGCAGGAAGAGAATCATTACTGAAGGCGACATCGCCACTCTTTTGCTGAGATATGATATGGAGATGATAC TGAGAATGCTACAGGAGATATCTTATTGTTCCGAAACCAAGATGGACTGGAATGCGTTGGTGAAGAAGACCACTACCGGAATTACTAATGCTAGAGAGTA CCAGTTGCTATGGCGTCATCTTTCTTATCGGCATCCTCTCCTCCCTGTGGAAGATGATGCTCTACCTCTGGACGACGATAGTGACATGGAGTGCGAATTG GAAGCTTCTCCTGCAGTCAGCCATGAAGCATCAGTGGAGGCTATTGCACATGTCAAAGTGATGGCTGCTTCATATGTTCTAAGTGAGTCTGATATACTCG ACGATTCAACAGTTGAGGCTCCCTTGACTATAAACATACCTTATGCTTTGCCTGAGGGTTCTCAGGAACCATCAGAGTCTCCTTGGTCGTCAAGAGGGAT GAATATCAACTTTCCGGTCTGTCTTCAGAAAGTTACATCTACCGAGGGGATGAATGGAAATGGTTCAGCTGGTATTAGCATGGCTTTTCGGAGGAAAAGG AAAAGATGGTCTGCTGAGGAGGATGAGGAGCTGTTCGCCGCTGTAAAGCGATGTGGTGAAGGGAACTGGGCTCATATTGTTAAGGGAGACTTTAGAGGAG AGAGAACCGCCTCCCAACTCTCGCAGAGGTGGGCGCTTATAAGAAAAAGGTGTCACACTTCGACCTCTGTTAGCCAATGTGGCCTACAAGGAACTGAAGC GAAACTAGCAGTTAACCATGCATTATCTTTAGCTCTGGGAAATCGGCCCCCTTCAAATAAGCTTGCAATAGGTCTTATGCCAACGACGTCATCTTGTACC ATCACAGAAACGGAAGCGAATGGGGGAAGTTCTTCTCAAGGTCAACAACAGTCCAAACCAATTGTTCAAGCATTGCCTCGGGCAGGAACATCACTTCCGG CTGCAAAGTCTCGAGTTGTTAAAAAAACAACAGCAAGCTCCACTTCCAGATCGGATCTTATGGTAACAGCTAATTCAGTAGCTGCAGCTGCATGCATGGG TGATGTATTGACTGCTGCATCAGGACGAAAGGTCGAACCTGGAAAAACTGATGCTCCACGAGTGCCAAAGACTAAACCTGTAAAACATGCTTCTACAGTC TGCATGCCTCAGCCCTCAGGTAGCCTCTCCATGCCAAAGGTTGAACCAGGAACGAGTGTTGCCGCCTCTATACGGTCTCTAGCTAATGGAAAATTGAAAC CTGTTATGGCTTCATCATCTTCCAACAAACCTCCTCTCATAGCTCCTCGTTCAGAAGGATCTTCAATGCTTTCTGCTTCCGCCCCCTTGGCTTCTCTATC AAGGATTGTCTCCAATCAGAGAGTTTTTGCAGGCTCTGTCCCAGCTACTGAGATTGTCACTTGCAAACCAGATGGTGGACAGAAAGGGCAAGCTCGTGGA AATGAAGCAAGCTCATCGGCTGCAATCCAGCCACATCAAATAACCTCAAGAAACTTGGAGATTAGCCAGGGAAAGCAGGCTACACAGGCTCAGTCCCCTA ATCTCTTGCCTAGGAAAGTTCCAGTAGTTCGGACTGCAGTTCATTGTGCCACTAACCAAAAGTTGATGGATAAACCATCTGATCAAACTGTAGTACCTAT CAGAGGAGCTGGTTCGCAATCTAAAGCCAAAGGTGAAGTAAACAGTAAGGTTGGTCCGGTGATCAAAGTGAGTAGTGTTTGCGGAAAACCCCTTGAGGTT GCAACTGTGGCAGGGACCGGACAGGGTGTT >IE_05A11_translated MVANNNTSSNRRKRIITEGDIATLLLRYDMEMILRMLQEISYCSETKMDWNALVKKTTTGITNAREYQLLWRHLSYRHPLLPVEDDALPLDDDSDMECEL EASPAVSHEASVEAIAHVKVMAASYVLSESDILDDSTVEAPLTINIPYALPEGSQEPSESPWSSRGMNINFPVCLQKVTSTEGMNGNGSAGISMAFRRKR KRWSAEEDEELFAAVKRCGEGNWAHIVKGDFRGERTASQLSQRWALIRKRCHTSTSVSQCGLQGTEAKLAVNHALSLALGNRPPSNKLAIGLMPTTSSCT ITETEANGGSSSQGQQQSKPIVQALPRAGTSLPAAKSRVVKKTTASSTSRSDLMVTANSVAAAACMGDVLTAASGRKVEPGKTDAPRVPKTKPVKHASTV CMPQPSGSLSMPKVEPGTSVAASIRSLANGKLKPVMASSSSNKPPLIAPRSEGSSMLSASAPLASLSRIVSNQRVFAGSVPATEIVTCKPDGGQKGQARG NEASSSAAIQPHQITSRNLEISQGKQATQAQSPNLLPRKVPVVRTAVHCATNQKLMDKPSDQTVVPIRGAGSQSKAKGEVNSKVGPVIKVSSVCGKPLEV ATVAGTGQGV
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 1829/1830 (99.9%)（アミノ酸配列で609/610 (99.8%)）一致しました。 10 20 30 40 50 60 70 80 90 100 AT1G09 ATGGTTGCTAATAATAATACTAGTAGCAATCGCAGGAAGAGAATCATTACTGAAGGCGACATCGCCACTCTTTTGCTGAGATATGATATGGAGACGATAC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: ::::: IE_05A ATGGTTGCTAATAATAATACTAGTAGCAATCGCAGGAAGAGAATCATTACTGAAGGCGACATCGCCACTCTTTTGCTGAGATATGATATGGAGATGATAC 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT1G09 TGAGAATGCTACAGGAGATATCTTATTGTTCCGAAACCAAGATGGACTGGAATGCGTTGGTGAAGAAGACCACTACCGGAATTACTAATGCTAGAGAGTA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05A TGAGAATGCTACAGGAGATATCTTATTGTTCCGAAACCAAGATGGACTGGAATGCGTTGGTGAAGAAGACCACTACCGGAATTACTAATGCTAGAGAGTA 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT1G09 CCAGTTGCTATGGCGTCATCTTTCTTATCGGCATCCTCTCCTCCCTGTGGAAGATGATGCTCTACCTCTGGACGACGATAGTGACATGGAGTGCGAATTG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05A CCAGTTGCTATGGCGTCATCTTTCTTATCGGCATCCTCTCCTCCCTGTGGAAGATGATGCTCTACCTCTGGACGACGATAGTGACATGGAGTGCGAATTG 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 AT1G09 GAAGCTTCTCCTGCAGTCAGCCATGAAGCATCAGTGGAGGCTATTGCACATGTCAAAGTGATGGCTGCTTCATATGTTCTAAGTGAGTCTGATATACTCG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05A GAAGCTTCTCCTGCAGTCAGCCATGAAGCATCAGTGGAGGCTATTGCACATGTCAAAGTGATGGCTGCTTCATATGTTCTAAGTGAGTCTGATATACTCG 310 320 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 480 490 500 AT1G09 ACGATTCAACAGTTGAGGCTCCCTTGACTATAAACATACCTTATGCTTTGCCTGAGGGTTCTCAGGAACCATCAGAGTCTCCTTGGTCGTCAAGAGGGAT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05A ACGATTCAACAGTTGAGGCTCCCTTGACTATAAACATACCTTATGCTTTGCCTGAGGGTTCTCAGGAACCATCAGAGTCTCCTTGGTCGTCAAGAGGGAT 410 420 430 440 450 460 470 480 490 500 510 520 530 540 550 560 570 580 590 600 AT1G09 GAATATCAACTTTCCGGTCTGTCTTCAGAAAGTTACATCTACCGAGGGGATGAATGGAAATGGTTCAGCTGGTATTAGCATGGCTTTTCGGAGGAAAAGG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05A GAATATCAACTTTCCGGTCTGTCTTCAGAAAGTTACATCTACCGAGGGGATGAATGGAAATGGTTCAGCTGGTATTAGCATGGCTTTTCGGAGGAAAAGG 510 520 530 540 550 560 570 580 590 600 610 620 630 640 650 660 670 680 690 700 AT1G09 AAAAGATGGTCTGCTGAGGAGGATGAGGAGCTGTTCGCCGCTGTAAAGCGATGTGGTGAAGGGAACTGGGCTCATATTGTTAAGGGAGACTTTAGAGGAG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05A AAAAGATGGTCTGCTGAGGAGGATGAGGAGCTGTTCGCCGCTGTAAAGCGATGTGGTGAAGGGAACTGGGCTCATATTGTTAAGGGAGACTTTAGAGGAG 610 620 630 640 650 660 670 680 690 700 710 720 730 740 750 760 770 780 790 800 AT1G09 AGAGAACCGCCTCCCAACTCTCGCAGAGGTGGGCGCTTATAAGAAAAAGGTGTCACACTTCGACCTCTGTTAGCCAATGTGGCCTACAAGGAACTGAAGC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05A AGAGAACCGCCTCCCAACTCTCGCAGAGGTGGGCGCTTATAAGAAAAAGGTGTCACACTTCGACCTCTGTTAGCCAATGTGGCCTACAAGGAACTGAAGC 710 720 730 740 750 760 770 780 790 800 810 820 830 840 850 860 870 880 890 900 AT1G09 GAAACTAGCAGTTAACCATGCATTATCTTTAGCTCTGGGAAATCGGCCCCCTTCAAATAAGCTTGCAATAGGTCTTATGCCAACGACGTCATCTTGTACC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05A GAAACTAGCAGTTAACCATGCATTATCTTTAGCTCTGGGAAATCGGCCCCCTTCAAATAAGCTTGCAATAGGTCTTATGCCAACGACGTCATCTTGTACC 810 820 830 840 850 860 870 880 890 900 910 920 930 940 950 960 970 980 990 1000 AT1G09 ATCACAGAAACGGAAGCGAATGGGGGAAGTTCTTCTCAAGGTCAACAACAGTCCAAACCAATTGTTCAAGCATTGCCTCGGGCAGGAACATCACTTCCGG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05A ATCACAGAAACGGAAGCGAATGGGGGAAGTTCTTCTCAAGGTCAACAACAGTCCAAACCAATTGTTCAAGCATTGCCTCGGGCAGGAACATCACTTCCGG 910 920 930 940 950 960 970 980 990 1000 1010 1020 1030 1040 1050 1060 1070 1080 1090 1100 AT1G09 CTGCAAAGTCTCGAGTTGTTAAAAAAACAACAGCAAGCTCCACTTCCAGATCGGATCTTATGGTAACAGCTAATTCAGTAGCTGCAGCTGCATGCATGGG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05A CTGCAAAGTCTCGAGTTGTTAAAAAAACAACAGCAAGCTCCACTTCCAGATCGGATCTTATGGTAACAGCTAATTCAGTAGCTGCAGCTGCATGCATGGG 1010 1020 1030 1040 1050 1060 1070 1080 1090 1100 1110 1120 1130 1140 1150 1160 1170 1180 1190 1200 AT1G09 TGATGTATTGACTGCTGCATCAGGACGAAAGGTCGAACCTGGAAAAACTGATGCTCCACGAGTGCCAAAGACTAAACCTGTAAAACATGCTTCTACAGTC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05A TGATGTATTGACTGCTGCATCAGGACGAAAGGTCGAACCTGGAAAAACTGATGCTCCACGAGTGCCAAAGACTAAACCTGTAAAACATGCTTCTACAGTC 1110 1120 1130 1140 1150 1160 1170 1180 1190 1200 1210 1220 1230 1240 1250 1260 1270 1280 1290 1300 AT1G09 TGCATGCCTCAGCCCTCAGGTAGCCTCTCCATGCCAAAGGTTGAACCAGGAACGAGTGTTGCCGCCTCTATACGGTCTCTAGCTAATGGAAAATTGAAAC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05A TGCATGCCTCAGCCCTCAGGTAGCCTCTCCATGCCAAAGGTTGAACCAGGAACGAGTGTTGCCGCCTCTATACGGTCTCTAGCTAATGGAAAATTGAAAC 1210 1220 1230 1240 1250 1260 1270 1280 1290 1300 1310 1320 1330 1340 1350 1360 1370 1380 1390 1400 AT1G09 CTGTTATGGCTTCATCATCTTCCAACAAACCTCCTCTCATAGCTCCTCGTTCAGAAGGATCTTCAATGCTTTCTGCTTCCGCCCCCTTGGCTTCTCTATC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05A CTGTTATGGCTTCATCATCTTCCAACAAACCTCCTCTCATAGCTCCTCGTTCAGAAGGATCTTCAATGCTTTCTGCTTCCGCCCCCTTGGCTTCTCTATC 1310 1320 1330 1340 1350 1360 1370 1380 1390 1400 1410 1420 1430 1440 1450 1460 1470 1480 1490 1500 AT1G09 AAGGATTGTCTCCAATCAGAGAGTTTTTGCAGGCTCTGTCCCAGCTACTGAGATTGTCACTTGCAAACCAGATGGTGGACAGAAAGGGCAAGCTCGTGGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05A AAGGATTGTCTCCAATCAGAGAGTTTTTGCAGGCTCTGTCCCAGCTACTGAGATTGTCACTTGCAAACCAGATGGTGGACAGAAAGGGCAAGCTCGTGGA 1410 1420 1430 1440 1450 1460 1470 1480 1490 1500 1510 1520 1530 1540 1550 1560 1570 1580 1590 1600 AT1G09 AATGAAGCAAGCTCATCGGCTGCAATCCAGCCACATCAAATAACCTCAAGAAACTTGGAGATTAGCCAGGGAAAGCAGGCTACACAGGCTCAGTCCCCTA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05A AATGAAGCAAGCTCATCGGCTGCAATCCAGCCACATCAAATAACCTCAAGAAACTTGGAGATTAGCCAGGGAAAGCAGGCTACACAGGCTCAGTCCCCTA 1510 1520 1530 1540 1550 1560 1570 1580 1590 1600 1610 1620 1630 1640 1650 1660 1670 1680 1690 1700 AT1G09 ATCTCTTGCCTAGGAAAGTTCCAGTAGTTCGGACTGCAGTTCATTGTGCCACTAACCAAAAGTTGATGGATAAACCATCTGATCAAACTGTAGTACCTAT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05A ATCTCTTGCCTAGGAAAGTTCCAGTAGTTCGGACTGCAGTTCATTGTGCCACTAACCAAAAGTTGATGGATAAACCATCTGATCAAACTGTAGTACCTAT 1610 1620 1630 1640 1650 1660 1670 1680 1690 1700 1710 1720 1730 1740 1750 1760 1770 1780 1790 1800 AT1G09 CAGAGGAGCTGGTTCGCAATCTAAAGCCAAAGGTGAAGTAAACAGTAAGGTTGGTCCGGTGATCAAAGTGAGTAGTGTTTGCGGAAAACCCCTTGAGGTT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05A CAGAGGAGCTGGTTCGCAATCTAAAGCCAAAGGTGAAGTAAACAGTAAGGTTGGTCCGGTGATCAAAGTGAGTAGTGTTTGCGGAAAACCCCTTGAGGTT 1710 1720 1730 1740 1750 1760 1770 1780 1790 1800 1810 1820 1830 AT1G09 GCAACTGTGGCAGGGACCGGACAGGGTGTT :::::::::::::::::::::::::::::: IE_05A GCAACTGTGGCAGGGACCGGACAGGGTGTT 1810 1820 1830 10 20 30 40 50 60 70 80 90 100 AT1G09 MVANNNTSSNRRKRIITEGDIATLLLRYDMETILRMLQEISYCSETKMDWNALVKKTTTGITNAREYQLLWRHLSYRHPLLPVEDDALPLDDDSDMECEL ::::::::::::::::::::::::::::::: :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05A MVANNNTSSNRRKRIITEGDIATLLLRYDMEMILRMLQEISYCSETKMDWNALVKKTTTGITNAREYQLLWRHLSYRHPLLPVEDDALPLDDDSDMECEL 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT1G09 EASPAVSHEASVEAIAHVKVMAASYVLSESDILDDSTVEAPLTINIPYALPEGSQEPSESPWSSRGMNINFPVCLQKVTSTEGMNGNGSAGISMAFRRKR :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05A EASPAVSHEASVEAIAHVKVMAASYVLSESDILDDSTVEAPLTINIPYALPEGSQEPSESPWSSRGMNINFPVCLQKVTSTEGMNGNGSAGISMAFRRKR 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT1G09 KRWSAEEDEELFAAVKRCGEGNWAHIVKGDFRGERTASQLSQRWALIRKRCHTSTSVSQCGLQGTEAKLAVNHALSLALGNRPPSNKLAIGLMPTTSSCT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05A KRWSAEEDEELFAAVKRCGEGNWAHIVKGDFRGERTASQLSQRWALIRKRCHTSTSVSQCGLQGTEAKLAVNHALSLALGNRPPSNKLAIGLMPTTSSCT 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 AT1G09 ITETEANGGSSSQGQQQSKPIVQALPRAGTSLPAAKSRVVKKTTASSTSRSDLMVTANSVAAAACMGDVLTAASGRKVEPGKTDAPRVPKTKPVKHASTV :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05A ITETEANGGSSSQGQQQSKPIVQALPRAGTSLPAAKSRVVKKTTASSTSRSDLMVTANSVAAAACMGDVLTAASGRKVEPGKTDAPRVPKTKPVKHASTV 310 320 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 480 490 500 AT1G09 CMPQPSGSLSMPKVEPGTSVAASIRSLANGKLKPVMASSSSNKPPLIAPRSEGSSMLSASAPLASLSRIVSNQRVFAGSVPATEIVTCKPDGGQKGQARG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05A CMPQPSGSLSMPKVEPGTSVAASIRSLANGKLKPVMASSSSNKPPLIAPRSEGSSMLSASAPLASLSRIVSNQRVFAGSVPATEIVTCKPDGGQKGQARG 410 420 430 440 450 460 470 480 490 500 510 520 530 540 550 560 570 580 590 600 AT1G09 NEASSSAAIQPHQITSRNLEISQGKQATQAQSPNLLPRKVPVVRTAVHCATNQKLMDKPSDQTVVPIRGAGSQSKAKGEVNSKVGPVIKVSSVCGKPLEV :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05A NEASSSAAIQPHQITSRNLEISQGKQATQAQSPNLLPRKVPVVRTAVHCATNQKLMDKPSDQTVVPIRGAGSQSKAKGEVNSKVGPVIKVSSVCGKPLEV 510 520 530 540 550 560 570 580 590 600 610 AT1G09 ATVAGTGQGV :::::::::: IE_05A ATVAGTGQGV 610

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