◆◆◆IE_05B03 detail◆◆◆

Code name	IE_05B03
Locus	AT4G37650
Template	IT_05B03 (HR0757)
Donor Vector	pDONR207
Forward primer	IPF_05B03 (ggggacaagtttgtacaaaaaagcaggcttcATGGATGAAGAAGACCTTTCCTCTTCTT)
Reverse primer	IPR_05B03 (ggggaccactttgtacaagaaagctgggttCGTTGGCCGCCACGCACTAGCCCAAACCAC)
Alignment with template seq.	1st	配列解析が行えなかったサンプルです。	2nd	アミノ酸配列100%一致
Alignment with TAIR7CDS	1st	-	2nd	アミノ酸配列59.10%一致
comment	1st:(Sequence解析まで行ったが、読み直す必要がある), 2nd:Templateの配列とは一致
sequence	>IE_05B03_1419bp ATGGATGAAGAAGACCTTTCCTCTTCTTCTTCTCACCACAACCATCACAACCACAACAATCCTAATACTTACTACTCTCCTTTCACTACTCCCACCCAAT ACCATCCCGCCACATCATCAACCCCTTCCTCCACCGCCGCAGCCGCAGCTTTAGCCTCGCCTTACTCCTCCTCCGGCCACCATAATGACCCTTCCGCGTT CTCCATACCTCAAACTCCTCCGTCCTTCGACTTCTCAGCCAATGCCAAGTGGGCAGACTCGGTCCTTCTTGAAGCGGCACGTGCCTTCTCCGACAAAGAC ACTGCACGTGCGCAACAAATCCTATGGACGCTCAACGAGCTCTCTTCTCCGTACGGAGACACCGAGCAAAAACTGGCTTCTTACTTCCTCCAAGCTCTCT TCAACCGCATGACCGGTTCAGGCGAACGATGCTACCGAACCATGGTAACAGCTGCAGCCACAGAGAAGACTTGCTCCTTCGAGTCAACGCGAAAAACTGT ACTAAAGTTCCAAGAAGTTAGCTCCTGGGCCACGTTTGGACACGTGGCGGCAAACGGAGCAATCTTGGAAGCAGTAGACGGAGAGGCAAAGATCCACATC GTTGACATAAGCTCCACGTTTTGCACTCAATGGCCGACTCTTCTAGAAGCTTTAGCCACAAGATCAGACGACACGCCTCACCTAAGGCTAACCACAGTTG TCGTGGCCAACAAGTTTGTCAACGATCAAACGGCGTCGCATCGGATGATGAAAGAGATCGGAAACCGAATGGAGAAATTCGCTAGGCTTATGGGAGTTCC TTTCAAATTTAACATTATTCATCACGTTGGAGATTTATCTGAGTTTGATCTCAACGAACTCGACGTTAAACCAGACGAAGTCTTGGCCATTAACTGCGTA GGCGCGATGCATGGGATCGCTTCACGTGGAAGCCCTAGAGACGCTGTGATATCGAGTTTCCGACGGTTAAGACCGAGGATTGTGACGGTCGTAGAAGAAG AAGCTGATCTTGTCGGAGAAGAAGAAGGTGGCTTTGATGATGAGTTCTTGAGAGGGTTTGGAGAATGTTTACGATGGTTTAGGGTTTGCTTCGAGTCATG GGAAGAGAGTTTTCCAAGGACGAGCAACGAGAGGTTGATGCTAGAGCGTGCAGCGGGACGTGCGATCGTTGATCTTGTGGCTTGTGAGCCGTCGGATTCC ACGGAGAGGCGAGAGACAGCGAGGAAGTGGTCGAGGAGGATGAGGAATAGTGGGTTTGGAGCGGTGGGGTATAGTGATGAGGTGGCGGATGATGTCAGAG CTTTGTTGAGGAGATATAAAGAAGGTGTTTGGTCGATGGTACAGTGTCCTGATGCCGCCGGAATATTCCTTTGTTGGAGAGATCAGCCGGTGGTTTGGGC TAGTGCGTGGCGGCCAACG >IE_05B03_translated MDEEDLSSSSSHHNHHNHNNPNTYYSPFTTPTQYHPATSSTPSSTAAAAALASPYSSSGHHNDPSAFSIPQTPPSFDFSANAKWADSVLLEAARAFSDKD TARAQQILWTLNELSSPYGDTEQKLASYFLQALFNRMTGSGERCYRTMVTAAATEKTCSFESTRKTVLKFQEVSSWATFGHVAANGAILEAVDGEAKIHI VDISSTFCTQWPTLLEALATRSDDTPHLRLTTVVVANKFVNDQTASHRMMKEIGNRMEKFARLMGVPFKFNIIHHVGDLSEFDLNELDVKPDEVLAINCV GAMHGIASRGSPRDAVISSFRRLRPRIVTVVEEEADLVGEEEGGFDDEFLRGFGECLRWFRVCFESWEESFPRTSNERLMLERAAGRAIVDLVACEPSDS TERRETARKWSRRMRNSGFGAVGYSDEVADDVRALLRRYKEGVWSMVQCPDAAGIFLCWRDQPVVWASAWRPT
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 1418/1593 (89.0%)（アミノ酸配列で472/531 (88.9%)）一致しました。 10 20 30 40 50 60 70 80 90 100 AT4G37 ATGGATACTCTCTTTAGACTAGTCAGTCTCCAACAACAACAACAATCCGATAGTATCATTACAAATCAATCTTCGTTAAGCAGAACTTCCACCACCACTA IE_05B ---------------------------------------------------------------------------------------------------- 110 120 130 140 150 160 170 180 190 200 AT4G37 CTGGCTCTCCACAAACTGCTTATCACTACAACTTTCCACAAAACGACGTCGTCGAAGAATGCTTCAACTTTTTCATGGATGAAGAAGACCTTTCCTCTTC :::::::::::::::::::::::::: IE_05B --------------------------------------------------------------------------ATGGATGAAGAAGACCTTTCCTCTTC 10 20 210 220 230 240 250 260 270 280 290 300 AT4G37 TTCTTCTCACCACAACCATCACAACCACAACAATCCTAATACTTACTACTCTCCTTTCACTACTCCCACCCAATACCATCCCGCCACATCATCAACCCCT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05B TTCTTCTCACCACAACCATCACAACCACAACAATCCTAATACTTACTACTCTCCTTTCACTACTCCCACCCAATACCATCCCGCCACATCATCAACCCCT 30 40 50 60 70 80 90 100 110 120 310 320 330 340 350 360 370 380 390 400 AT4G37 TCCTCCACCGCCGCAGCCGCAGCTTTAGCCTCGCCTTACTCCTCCTCCGGCCACCATAATGACCCTTCCGCGTTCTCCATACCTCAAACTCCTCCGTCCT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05B TCCTCCACCGCCGCAGCCGCAGCTTTAGCCTCGCCTTACTCCTCCTCCGGCCACCATAATGACCCTTCCGCGTTCTCCATACCTCAAACTCCTCCGTCCT 130 140 150 160 170 180 190 200 210 220 410 420 430 440 450 460 470 480 490 500 AT4G37 TCGACTTCTCAGCCAATGCCAAGTGGGCAGACTCGGTCCTTCTTGAAGCGGCACGTGCCTTCTCCGACAAAGACACTGCACGTGCGCAACAAATCCTATG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05B TCGACTTCTCAGCCAATGCCAAGTGGGCAGACTCGGTCCTTCTTGAAGCGGCACGTGCCTTCTCCGACAAAGACACTGCACGTGCGCAACAAATCCTATG 230 240 250 260 270 280 290 300 310 320 510 520 530 540 550 560 570 580 590 600 AT4G37 GACGCTCAACGAGCTCTCTTCTCCGTACGGAGACACCGAGCAAAAACTGGCTTCTTACTTCCTCCAAGCTCTCTTCAACCGCATGACCGGTTCAGGCGAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05B GACGCTCAACGAGCTCTCTTCTCCGTACGGAGACACCGAGCAAAAACTGGCTTCTTACTTCCTCCAAGCTCTCTTCAACCGCATGACCGGTTCAGGCGAA 330 340 350 360 370 380 390 400 410 420 610 620 630 640 650 660 670 680 690 700 AT4G37 CGATGCTACCGAACCATGGTAACAGCTGCAGCCACAGAGAAGACTTGCTCCTTCGAGTCAACGCGAAAAACTGTACTAAAGTTCCAAGAAGTTAGCCCCT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: ::: IE_05B CGATGCTACCGAACCATGGTAACAGCTGCAGCCACAGAGAAGACTTGCTCCTTCGAGTCAACGCGAAAAACTGTACTAAAGTTCCAAGAAGTTAGCTCCT 430 440 450 460 470 480 490 500 510 520 710 720 730 740 750 760 770 780 790 800 AT4G37 GGGCCACGTTTGGACACGTGGCGGCAAACGGAGCAATCTTGGAAGCAGTAGACGGAGAGGCAAAGATCCACATCGTTGACATAAGCTCCACGTTTTGCAC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05B GGGCCACGTTTGGACACGTGGCGGCAAACGGAGCAATCTTGGAAGCAGTAGACGGAGAGGCAAAGATCCACATCGTTGACATAAGCTCCACGTTTTGCAC 530 540 550 560 570 580 590 600 610 620 810 820 830 840 850 860 870 880 890 900 AT4G37 TCAATGGCCGACTCTTCTAGAAGCTTTAGCCACAAGATCAGACGACACGCCTCACCTAAGGCTAACCACAGTTGTCGTGGCCAACAAGTTTGTCAACGAT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05B TCAATGGCCGACTCTTCTAGAAGCTTTAGCCACAAGATCAGACGACACGCCTCACCTAAGGCTAACCACAGTTGTCGTGGCCAACAAGTTTGTCAACGAT 630 640 650 660 670 680 690 700 710 720 910 920 930 940 950 960 970 980 990 1000 AT4G37 CAAACGGCGTCGCATCGGATGATGAAAGAGATCGGAAACCGAATGGAGAAATTCGCTAGGCTTATGGGAGTTCCTTTCAAATTTAACATTATTCATCACG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05B CAAACGGCGTCGCATCGGATGATGAAAGAGATCGGAAACCGAATGGAGAAATTCGCTAGGCTTATGGGAGTTCCTTTCAAATTTAACATTATTCATCACG 730 740 750 760 770 780 790 800 810 820 1010 1020 1030 1040 1050 1060 1070 1080 1090 1100 AT4G37 TTGGAGATTTATCTGAGTTTGATCTCAACGAACTCGACGTTAAACCAGACGAAGTCTTGGCCATTAACTGCGTAGGCGCGATGCATGGGATCGCTTCACG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05B TTGGAGATTTATCTGAGTTTGATCTCAACGAACTCGACGTTAAACCAGACGAAGTCTTGGCCATTAACTGCGTAGGCGCGATGCATGGGATCGCTTCACG 830 840 850 860 870 880 890 900 910 920 1110 1120 1130 1140 1150 1160 1170 1180 1190 1200 AT4G37 TGGAAGCCCTAGAGACGCTGTGATATCGAGTTTCCGACGGTTAAGACCGAGGATTGTGACGGTCGTAGAAGAAGAAGCTGATCTTGTCGGAGAAGAAGAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05B TGGAAGCCCTAGAGACGCTGTGATATCGAGTTTCCGACGGTTAAGACCGAGGATTGTGACGGTCGTAGAAGAAGAAGCTGATCTTGTCGGAGAAGAAGAA 930 940 950 960 970 980 990 1000 1010 1020 1210 1220 1230 1240 1250 1260 1270 1280 1290 1300 AT4G37 GGTGGCTTTGATGATGAGTTCTTGAGAGGGTTTGGAGAATGTTTACGATGGTTTAGGGTTTGCTTCGAGTCATGGGAAGAGAGTTTTCCAAGGACGAGCA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05B GGTGGCTTTGATGATGAGTTCTTGAGAGGGTTTGGAGAATGTTTACGATGGTTTAGGGTTTGCTTCGAGTCATGGGAAGAGAGTTTTCCAAGGACGAGCA 1030 1040 1050 1060 1070 1080 1090 1100 1110 1120 1310 1320 1330 1340 1350 1360 1370 1380 1390 1400 AT4G37 ACGAGAGGTTGATGCTAGAGCGTGCAGCGGGACGTGCGATCGTTGATCTTGTGGCTTGTGAGCCGTCGGATTCCACGGAGAGGCGAGAGACAGCGAGGAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05B ACGAGAGGTTGATGCTAGAGCGTGCAGCGGGACGTGCGATCGTTGATCTTGTGGCTTGTGAGCCGTCGGATTCCACGGAGAGGCGAGAGACAGCGAGGAA 1130 1140 1150 1160 1170 1180 1190 1200 1210 1220 1410 1420 1430 1440 1450 1460 1470 1480 1490 1500 AT4G37 GTGGTCGAGGAGGATGAGGAATAGTGGGTTTGGAGCGGTGGGGTATAGTGATGAGGTGGCGGATGATGTCAGAGCTTTGTTGAGGAGATATAAAGAAGGT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05B GTGGTCGAGGAGGATGAGGAATAGTGGGTTTGGAGCGGTGGGGTATAGTGATGAGGTGGCGGATGATGTCAGAGCTTTGTTGAGGAGATATAAAGAAGGT 1230 1240 1250 1260 1270 1280 1290 1300 1310 1320 1510 1520 1530 1540 1550 1560 1570 1580 1590 AT4G37 GTTTGGTCGATGGTACAGTGTCCTGATGCCGCCGGAATATTCCTTTGTTGGAGAGATCAGCCGGTGGTTTGGGCTAGTGCGTGGCGGCCAACG ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05B GTTTGGTCGATGGTACAGTGTCCTGATGCCGCCGGAATATTCCTTTGTTGGAGAGATCAGCCGGTGGTTTGGGCTAGTGCGTGGCGGCCAACG 1330 1340 1350 1360 1370 1380 1390 1400 1410 10 20 30 40 50 60 70 80 90 100 AT4G37 MDTLFRLVSLQQQQQSDSIITNQSSLSRTSTTTTGSPQTAYHYNFPQNDVVEECFNFFMDEEDLSSSSSHHNHHNHNNPNTYYSPFTTPTQYHPATSSTP :::::::::::::::::::::::::::::::::::::::::: IE_05B ----------------------------------------------------------MDEEDLSSSSSHHNHHNHNNPNTYYSPFTTPTQYHPATSSTP 10 20 30 40 110 120 130 140 150 160 170 180 190 200 AT4G37 SSTAAAAALASPYSSSGHHNDPSAFSIPQTPPSFDFSANAKWADSVLLEAARAFSDKDTARAQQILWTLNELSSPYGDTEQKLASYFLQALFNRMTGSGE :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05B SSTAAAAALASPYSSSGHHNDPSAFSIPQTPPSFDFSANAKWADSVLLEAARAFSDKDTARAQQILWTLNELSSPYGDTEQKLASYFLQALFNRMTGSGE 50 60 70 80 90 100 110 120 130 140 210 220 230 240 250 260 270 280 290 300 AT4G37 RCYRTMVTAAATEKTCSFESTRKTVLKFQEVSPWATFGHVAANGAILEAVDGEAKIHIVDISSTFCTQWPTLLEALATRSDDTPHLRLTTVVVANKFVND :::::::::::::::::::::::::::::::: ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05B RCYRTMVTAAATEKTCSFESTRKTVLKFQEVSSWATFGHVAANGAILEAVDGEAKIHIVDISSTFCTQWPTLLEALATRSDDTPHLRLTTVVVANKFVND 150 160 170 180 190 200 210 220 230 240 310 320 330 340 350 360 370 380 390 400 AT4G37 QTASHRMMKEIGNRMEKFARLMGVPFKFNIIHHVGDLSEFDLNELDVKPDEVLAINCVGAMHGIASRGSPRDAVISSFRRLRPRIVTVVEEEADLVGEEE :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05B QTASHRMMKEIGNRMEKFARLMGVPFKFNIIHHVGDLSEFDLNELDVKPDEVLAINCVGAMHGIASRGSPRDAVISSFRRLRPRIVTVVEEEADLVGEEE 250 260 270 280 290 300 310 320 330 340 410 420 430 440 450 460 470 480 490 500 AT4G37 GGFDDEFLRGFGECLRWFRVCFESWEESFPRTSNERLMLERAAGRAIVDLVACEPSDSTERRETARKWSRRMRNSGFGAVGYSDEVADDVRALLRRYKEG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05B GGFDDEFLRGFGECLRWFRVCFESWEESFPRTSNERLMLERAAGRAIVDLVACEPSDSTERRETARKWSRRMRNSGFGAVGYSDEVADDVRALLRRYKEG 350 360 370 380 390 400 410 420 430 440 510 520 530 AT4G37 VWSMVQCPDAAGIFLCWRDQPVVWASAWRPT ::::::::::::::::::::::::::::::: IE_05B VWSMVQCPDAAGIFLCWRDQPVVWASAWRPT 450 460 470

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