◆◆◆IE_05C04（IE_09B08） detail◆◆◆

Code nameIE_05C04（IE_09B08）
LocusAT1G01930
TemplateIT_05C04 (HR1160)
Donor VectorpDONR207
Forward primerIPF_05C04 (ggggacaagtttgtacaaaaaagcaggcttcATGGCGACCACCGGCGCGGCGGCCACCA)
Reverse primerIPR_05C04 (ggggaccactttgtacaagaaagctgggttACTTGTGCTTGAACTGCTTTGAATGTTAAG)
Alignment with template seq.1stアミノ酸配列99.80%一致2ndアミノ酸配列100%一致
Alignment with TAIR7CDSアミノ酸配列99.80%一致アミノ酸配列100%一致
comment1st:(Sequence解析まで行ったが、フレームはずれないが塩基置換がある)→2nd(09B08):100%
sequence 
>IE_05C04_1740bp
ATGGCGACCACCGGCGCGGCGGCCACCACCGAGATTGGTAATCCAGAATATAAACAGCCTCGATCGATATTTGACTTGACGGCGGATTTCTTTGACTCGT
GTCGGTTGTCGAATCCTTCGGAGACTCAATCGGCATTCGGACCACCCAAAACTTTCGATCCAGAGGAGGCGGAGGAGGATAAGAGTTCCAAGGACGGCGT
GATTTTGGACAGATGGACCTGTAATACTTGTAAAATTGAGTTCCTGTCTCTTCAAGATCAACGCTACCACTTCAAGTCCGATATTCATCGCCTTAACATC
AAGCTGAGTGTTGCTGGCAAGGCTATATTAAAAGAGGAAGATGTTGATGAGTTGACATCTGAGTCGGTCCAAGACTATGACGTGTCTAGCATATCAGGTT
CAGAGGACGAAGCTGAGACTAGACCACCCTCTTTCCACTTTGATGCTCAAAAGGGTATTGATAAAAAGAAACTATTTTTCCGTCTTCAAAGTGGTGACAA
AGTTTCTATCTGGAAATGCTTAATCATGGACGACGCTGAAAGTGTTTCGTTCGAGAATGACAGAGGAGTTTCTGTTGACTGTTGTGGTAGCTTGGTCGAG
AATGAAGTTACTGAGAGGTTGAGAAATTTGATTCGAGAGAATAAGGATGATAGGCAAATGCGTGTCGTGTTGCTTGCAAGTGGTGGCCACTTTGCCGGTA
CTGTCTTTAATGGAAAGTCGGTTGTGGCTCATAAGACATTCCACAGATATGTTGTAAGAGCCAAGGCTGGTAAAAAGCAGTCCACAAAAGATGCAAGCGG
GAGAAGTATACACTCTGCCGGAGCCTCACTTCGTCGTTACAATGAACTTGCTTTAAAAAAGGATATTCAAGAGCTACTTGCTAGCTGGAAACCTTATTTT
GATGGTGCTGCTTGTGTTTTTGTCCATGCTCCCTCAAGCAGTAGGCAATTACTTTTCAATGGAGGCAAACCGTACTTTAGCAGCCAAAACTGCGCTGTAC
GAAACGTTCCATTTACGATCCGTCGGCCAACCTTCAAGGAATCACAGCGTATATATAATCAGTTGACTCAGATTGCACATGTAACTGAGGAGATTTTTGT
TAATCGACCAGAAGTCACCAAAGCTAATACAGTGGTGCAGACCCACAATGAGGATTCTGGCAAAACCTCAAGAAAGGAAGAGCCCGATGAAACTTCTTCT
AGCAACATAATCTTAGAGGAACCAAATCGTATAGAAGAGGATATCGAGGATGGAGTTACTGGTACGTCAACTGCCTTACATGAAGCTGCCAAATCGGGTG
ATTGTGAAAGAGTTATGGAGTTCTTGGAGGAGGGTATGGATCCTTGTGCTAAGGATGAAAGAGGCAGAACGCCATACATGCTTGCAAATGAGAAGGAAGT
GAGAAACACTTTCAGACGGTTTATGGCGTTAAACCTTGAAAAATGGAATTGGCATGATGCTAAAGTACCCAGCCCGCTCACAAAAGAGATGGAGGAATCT
CAAGCTGCCAAGCAGGCAGAAAAAGACGCCAAGCAGAAAGCAAGAACAAAGGAGTTGAAGAAACTACGCAAAGCCCGAGAAAAGAAGGCTCAGGCCGAAG
CTGCGCAGGCGGAGAAGGAGAAGCCAATTTCTAAAGTGGAGGAAGTGAGGAGAGCAATGGCAGCACAGAGGGAAAAGAGAGCAGCCGCTGCAGAAAGACG
GATGGCATCACTTAACATTCAAAGCAGTTCAAGCACAAGT

>IE_05C04_translated
MATTGAAATTEIGNPEYKQPRSIFDLTADFFDSCRLSNPSETQSAFGPPKTFDPEEAEEDKSSKDGVILDRWTCNTCKIEFLSLQDQRYHFKSDIHRLNI
KLSVAGKAILKEEDVDELTSESVQDYDVSSISGSEDEAETRPPSFHFDAQKGIDKKKLFFRLQSGDKVSIWKCLIMDDAESVSFENDRGVSVDCCGSLVE
NEVTERLRNLIRENKDDRQMRVVLLASGGHFAGTVFNGKSVVAHKTFHRYVVRAKAGKKQSTKDASGRSIHSAGASLRRYNELALKKDIQELLASWKPYF
DGAACVFVHAPSSSRQLLFNGGKPYFSSQNCAVRNVPFTIRRPTFKESQRIYNQLTQIAHVTEEIFVNRPEVTKANTVVQTHNEDSGKTSRKEEPDETSS
SNIILEEPNRIEEDIEDGVTGTSTALHEAAKSGDCERVMEFLEEGMDPCAKDERGRTPYMLANEKEVRNTFRRFMALNLEKWNWHDAKVPSPLTKEMEES
QAAKQAEKDAKQKARTKELKKLRKAREKKAQAEAAQAEKEKPISKVEEVRRAMAAQREKRAAAAERRMASLNIQSSSSTS
Alignment ◆CDS（TAIRver.9）_without_stop_codonとのalignment◆

100%一致しました。

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