◆◆◆IE_05D09 detail◆◆◆

Code name	IE_05D09
Locus	AT5G10120
Template	IT_05D09 (HR2252)
Donor Vector	pDONR207
Forward primer	IPF_05D09 (ggggacaagtttgtacaaaaaagcaggcttcATGGTGGAAGTCGAAGAATTGGAACCAC)
Reverse primer	IPR_05D09 (ggggaccactttgtacaagaaagctgggttATCGAACATGTATATGTCTTTGTCCTCGCA)
Alignment with template seq.	1st	アミノ酸配列100%一致	2nd	-
Alignment with TAIR7CDS	1st	アミノ酸配列99.80%一致	2nd	-
comment	1st:Templateの配列とは一致
sequence	>IE_05D09_1413bp ATGGTGGAAGTCGAAGAATTGGAACCACTTAGTCCAATGGACGATGAAGAAGAAGAGATAAGCTATGATGACCTCAAAAGACGCATGTGGAAAGACCGAA ACCTCATGGAAAAAAAGCTCAAGCAACAGAAACGACACAGCAACGACGTCGTTTCGTTTACAACACACCGAGCCGAAGCTTCACGGCGCAAGAAGATGGC TCGTTCTCAAGATTCAGTGTTGAAGTACATGATGAAGATCATGGAGGTGTGTAAAGCTAAAGGTTTTGTATACGGAATTGTACCGGAAAAGGGTAAACCA ATAACCGGTTCTTCCGATAGCTTAAGACGTTGGTGGAAAGAAAACGTTCAGTTCGACCAAAACGCTCCAGACGCTATCACAGATTACTTAGCACTCGCGG CTGCTGCTGCTGCAGCAGAGCTAATCGATAAGTCCTCATCATCCTCGAGCTTATTACACATGTTACAGGAACTGCAAGACACGACACTAGGGTCGTTACT ATCAGCGTTAATGCAACATTGCATGCCGCCACAGCGGCGGTTTCCGTTAGAGAAAGGAATTGCTCCGCCATGGTGGCCGACGGGGACGGAGCTTTGGTGG GGAGAACAAGGAGCGGCACATGAGCACGGTGCACCGCCATATCGAAAGCCGCACGATTTGAGAAAATCTTGGAAAGTTAGTGTTCTCGCGGCTGTGATTA AACACATGTCGCCGAATTTGGGAAGAGTGAGACGTCTGGCGAGACAATCTAAAAGTTTGCAAGATAAAATGATGGCTAAAGAGACTGATACTTGGTCTCG TGTCTTAAACCAAGAAGAAGCTCTTCTCAATATCAAAGACCTCAAGATCTCGGAAGATCAGGACGATCAAGAATCGTCGGGGTCTAAGAGAAAAAGTGAG TCTATGGAACCGTCTAAGAGTGTTTACACTTGCCAAAACTCGAGTTGTCCTAAAAGTGATGTGAGTTTTGGATTCGGGGACAAGAACTCGAGGACGGGTC ATGAGATCCAGTGTCTTTATGGGTCGAACCAAGAACCGAGCCAGAGCGGTGAATATACATCTTCCTTGCTTGGAACGGTTCCAAGTATTGTTACTAATTC GACTAGTGAAGATGATTATTACAATGTGAGCTCGCGTGCACTGGACAAGCGAGATGATGATGATCATAGCATTAATGGGAATTGGATGGAATATTTCTGG TTAGAAAAGATGCAGCAGGAGTTTCATTGCTCTAGGAGATTCGAAGATGATGAGGGTACTGGAACGGACTTTGACCAACTCACAGAATCTGATCGATCGG ACAACGTGAATTTGAACCAACTTACAAAATCTGATCGATCGGACAACGTGAACCGGAGTGCTTTTTCAGTTTGGGACATGGGTTGCGAGGACAAAGACAT ATACATGTTCGAT >IE_05D09_translated MVEVEELEPLSPMDDEEEEISYDDLKRRMWKDRNLMEKKLKQQKRHSNDVVSFTTHRAEASRRKKMARSQDSVLKYMMKIMEVCKAKGFVYGIVPEKGKP ITGSSDSLRRWWKENVQFDQNAPDAITDYLALAAAAAAAELIDKSSSSSSLLHMLQELQDTTLGSLLSALMQHCMPPQRRFPLEKGIAPPWWPTGTELWW GEQGAAHEHGAPPYRKPHDLRKSWKVSVLAAVIKHMSPNLGRVRRLARQSKSLQDKMMAKETDTWSRVLNQEEALLNIKDLKISEDQDDQESSGSKRKSE SMEPSKSVYTCQNSSCPKSDVSFGFGDKNSRTGHEIQCLYGSNQEPSQSGEYTSSLLGTVPSIVTNSTSEDDYYNVSSRALDKRDDDDHSINGNWMEYFW LEKMQQEFHCSRRFEDDEGTGTDFDQLTESDRSDNVNLNQLTKSDRSDNVNRSAFSVWDMGCEDKDIYMFD
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 1412/1413 (99.9%)（アミノ酸配列で470/471 (99.8%)）一致しました。 10 20 30 40 50 60 70 80 90 100 AT5G10 ATGGTGGAAGTCGAAGAATTGGAACCACTTAGTCCAATGGACGATGAAGAAGAAGAGATAAGCTATGATGACCTCAAAAGACGCATGTGGAAAGACCGAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05D ATGGTGGAAGTCGAAGAATTGGAACCACTTAGTCCAATGGACGATGAAGAAGAAGAGATAAGCTATGATGACCTCAAAAGACGCATGTGGAAAGACCGAA 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT5G10 ACCTCATGGAAAAAAAGCTCAAGCAACAGAAACGACACAGCAACGACGTCGTTTCGTTTACAACACACCGAGCCGAAGCTTCACGGCGCAAGAAGATGGC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05D ACCTCATGGAAAAAAAGCTCAAGCAACAGAAACGACACAGCAACGACGTCGTTTCGTTTACAACACACCGAGCCGAAGCTTCACGGCGCAAGAAGATGGC 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT5G10 TCGTTCTCAAGATTCAGTGTTGAAGTACATGATGAAGATCATGGAGGTGTGTAAAGCTAAAGGTTTTGTATACGGAATTGTACCGGAAAAGGGTAAACCA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05D TCGTTCTCAAGATTCAGTGTTGAAGTACATGATGAAGATCATGGAGGTGTGTAAAGCTAAAGGTTTTGTATACGGAATTGTACCGGAAAAGGGTAAACCA 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 AT5G10 ATAACCGGTTCTTCCGATAGCTTAAGACGTTGGTGGAAAGAAAACGTTCAGTTCGACCAAAACGCTCCAGACGCTATCACAGATTACTTAGCACTCGCGG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05D ATAACCGGTTCTTCCGATAGCTTAAGACGTTGGTGGAAAGAAAACGTTCAGTTCGACCAAAACGCTCCAGACGCTATCACAGATTACTTAGCACTCGCGG 310 320 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 480 490 500 AT5G10 CTGCTGCTGCTGCAGCAGAGCTAATCGATAAGTCCTCATCATCCTCGAGCTTATTACACATGTTACAGGAACTGCAAGACACGACACTAGGGTCGTTACT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05D CTGCTGCTGCTGCAGCAGAGCTAATCGATAAGTCCTCATCATCCTCGAGCTTATTACACATGTTACAGGAACTGCAAGACACGACACTAGGGTCGTTACT 410 420 430 440 450 460 470 480 490 500 510 520 530 540 550 560 570 580 590 600 AT5G10 ATCAGCGTTAATGCAACATTGCATGCCGCCACAGCGGCGGTTTCCGTTAGAGAAAGGAATTGCTCCGCCATGGTGGCCGACGGGGACGGAGCTTTGGTGG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05D ATCAGCGTTAATGCAACATTGCATGCCGCCACAGCGGCGGTTTCCGTTAGAGAAAGGAATTGCTCCGCCATGGTGGCCGACGGGGACGGAGCTTTGGTGG 510 520 530 540 550 560 570 580 590 600 610 620 630 640 650 660 670 680 690 700 AT5G10 GGAGAACAAGGAGCGGCACATGAGCACGGTGCACCGCCATATCGAAAGCCGCACGATTTGAGAAAATCTTGGAAAGTTAGTGTTCTCGCGGCTGTGATTA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05D GGAGAACAAGGAGCGGCACATGAGCACGGTGCACCGCCATATCGAAAGCCGCACGATTTGAGAAAATCTTGGAAAGTTAGTGTTCTCGCGGCTGTGATTA 610 620 630 640 650 660 670 680 690 700 710 720 730 740 750 760 770 780 790 800 AT5G10 AACACATGTCGCCGAATTTGGGAAGAGTGAGACGTCTGGCGAGACAATCTAAAAGTTTGCAAGATAAAATGATGGCTAAAGAGACTGATACTTGGTCTCG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05D AACACATGTCGCCGAATTTGGGAAGAGTGAGACGTCTGGCGAGACAATCTAAAAGTTTGCAAGATAAAATGATGGCTAAAGAGACTGATACTTGGTCTCG 710 720 730 740 750 760 770 780 790 800 810 820 830 840 850 860 870 880 890 900 AT5G10 TGTCTTAAACCAAGAAGAAGCTCTTCTCAATATCAAAGACCTCAAGATCTCGGAAGATCAGGACGATCAAGAATCGTCGGGGTCTAAGAGAAAAAGTGAG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05D TGTCTTAAACCAAGAAGAAGCTCTTCTCAATATCAAAGACCTCAAGATCTCGGAAGATCAGGACGATCAAGAATCGTCGGGGTCTAAGAGAAAAAGTGAG 810 820 830 840 850 860 870 880 890 900 910 920 930 940 950 960 970 980 990 1000 AT5G10 TCTATGGAACCGTCTAAGAGTGTTTACACTTGCCAAAACTCGAGTTGTCCTAAAAGTGATGTGAGTTTTGGATTCGGGGACAAGAACTCGAGGACGGGTC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05D TCTATGGAACCGTCTAAGAGTGTTTACACTTGCCAAAACTCGAGTTGTCCTAAAAGTGATGTGAGTTTTGGATTCGGGGACAAGAACTCGAGGACGGGTC 910 920 930 940 950 960 970 980 990 1000 1010 1020 1030 1040 1050 1060 1070 1080 1090 1100 AT5G10 ATGAGATCCAGTGTCTTTATGGGTCGAACCAAGAACCGAGCCAGAGCGGTGAATATACATCTTCCTTGCTTGAAACGGTTCCAAGTATTGTTACTAATTC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: ::::::::::::::::::::::::::: IE_05D ATGAGATCCAGTGTCTTTATGGGTCGAACCAAGAACCGAGCCAGAGCGGTGAATATACATCTTCCTTGCTTGGAACGGTTCCAAGTATTGTTACTAATTC 1010 1020 1030 1040 1050 1060 1070 1080 1090 1100 1110 1120 1130 1140 1150 1160 1170 1180 1190 1200 AT5G10 GACTAGTGAAGATGATTATTACAATGTGAGCTCGCGTGCACTGGACAAGCGAGATGATGATGATCATAGCATTAATGGGAATTGGATGGAATATTTCTGG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05D GACTAGTGAAGATGATTATTACAATGTGAGCTCGCGTGCACTGGACAAGCGAGATGATGATGATCATAGCATTAATGGGAATTGGATGGAATATTTCTGG 1110 1120 1130 1140 1150 1160 1170 1180 1190 1200 1210 1220 1230 1240 1250 1260 1270 1280 1290 1300 AT5G10 TTAGAAAAGATGCAGCAGGAGTTTCATTGCTCTAGGAGATTCGAAGATGATGAGGGTACTGGAACGGACTTTGACCAACTCACAGAATCTGATCGATCGG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05D TTAGAAAAGATGCAGCAGGAGTTTCATTGCTCTAGGAGATTCGAAGATGATGAGGGTACTGGAACGGACTTTGACCAACTCACAGAATCTGATCGATCGG 1210 1220 1230 1240 1250 1260 1270 1280 1290 1300 1310 1320 1330 1340 1350 1360 1370 1380 1390 1400 AT5G10 ACAACGTGAATTTGAACCAACTTACAAAATCTGATCGATCGGACAACGTGAACCGGAGTGCTTTTTCAGTTTGGGACATGGGTTGCGAGGACAAAGACAT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05D ACAACGTGAATTTGAACCAACTTACAAAATCTGATCGATCGGACAACGTGAACCGGAGTGCTTTTTCAGTTTGGGACATGGGTTGCGAGGACAAAGACAT 1310 1320 1330 1340 1350 1360 1370 1380 1390 1400 1410 AT5G10 ATACATGTTCGAT ::::::::::::: IE_05D ATACATGTTCGAT 1410 10 20 30 40 50 60 70 80 90 100 AT5G10 MVEVEELEPLSPMDDEEEEISYDDLKRRMWKDRNLMEKKLKQQKRHSNDVVSFTTHRAEASRRKKMARSQDSVLKYMMKIMEVCKAKGFVYGIVPEKGKP :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05D MVEVEELEPLSPMDDEEEEISYDDLKRRMWKDRNLMEKKLKQQKRHSNDVVSFTTHRAEASRRKKMARSQDSVLKYMMKIMEVCKAKGFVYGIVPEKGKP 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT5G10 ITGSSDSLRRWWKENVQFDQNAPDAITDYLALAAAAAAAELIDKSSSSSSLLHMLQELQDTTLGSLLSALMQHCMPPQRRFPLEKGIAPPWWPTGTELWW :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05D ITGSSDSLRRWWKENVQFDQNAPDAITDYLALAAAAAAAELIDKSSSSSSLLHMLQELQDTTLGSLLSALMQHCMPPQRRFPLEKGIAPPWWPTGTELWW 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT5G10 GEQGAAHEHGAPPYRKPHDLRKSWKVSVLAAVIKHMSPNLGRVRRLARQSKSLQDKMMAKETDTWSRVLNQEEALLNIKDLKISEDQDDQESSGSKRKSE :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05D GEQGAAHEHGAPPYRKPHDLRKSWKVSVLAAVIKHMSPNLGRVRRLARQSKSLQDKMMAKETDTWSRVLNQEEALLNIKDLKISEDQDDQESSGSKRKSE 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 AT5G10 SMEPSKSVYTCQNSSCPKSDVSFGFGDKNSRTGHEIQCLYGSNQEPSQSGEYTSSLLETVPSIVTNSTSEDDYYNVSSRALDKRDDDDHSINGNWMEYFW ::::::::::::::::::::::::::::::::::::::::::::::::::::::::: :::::::::::::::::::::::::::::::::::::::::: IE_05D SMEPSKSVYTCQNSSCPKSDVSFGFGDKNSRTGHEIQCLYGSNQEPSQSGEYTSSLLGTVPSIVTNSTSEDDYYNVSSRALDKRDDDDHSINGNWMEYFW 310 320 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 AT5G10 LEKMQQEFHCSRRFEDDEGTGTDFDQLTESDRSDNVNLNQLTKSDRSDNVNRSAFSVWDMGCEDKDIYMFD ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05D LEKMQQEFHCSRRFEDDEGTGTDFDQLTESDRSDNVNLNQLTKSDRSDNVNRSAFSVWDMGCEDKDIYMFD 410 420 430 440 450 460 470

このページのTOPに戻る