◆◆◆IE_05E10 detail◆◆◆

Code name	IE_05E10
Locus	AT5G20730
Template	IT_05E10 (HR3115)
Donor Vector	pDONR207
Forward primer	IPF_05E10 (ggggacaagtttgtacaaaaaagcaggcttcATGAACAGACAACCTAATGAATTTTTCT)
Reverse primer	IPR_05E10 (ggggaccactttgtacaagaaagctgggttCCGGTTAAACGAAGTGGCTGAGTTATCATC)
Alignment with template seq.	1st	アミノ酸配列100%一致	2nd	-
Alignment with TAIR7CDS	1st	アミノ酸配列89.70%一致	2nd	-
comment	1st:Templateの配列とは一致
sequence	>IE_05E10_3138bp ATGAACAGACAACCTAATGAATTTTTCTGCAAAACCCTCACGGCGAGTGACACAAGTACTCACGGTGGATTTTCTGTACCCCGACGAGCTGCTGAGAAAA TCTTTCCTGCTCTGGATTTCTCGATGCAACCACCTTGTCAGGAGCTTGTTGCTAAGGATATTCATGACAACACATGGACTTTCAGACATATTTATCGAGG TCAACCAAAAAGGCACTTGCTAACTACAGGCTGGAGTGTGTTTGTCAGCACGAAAAGGCTCTTTGCTGGAGACTCTGTTCTTTTTATAAGAGATGGAAAG GCGCAACTTCTGTTGGGGATAAGACGTGCAAATAGACAACAGCCTGCACTTTCTTCATCTGTAATATCAAGTGATAGCATGCACATCGGAGTTCTTGCAG CTGCAGCTCATGCTAATGCTAATAACAGTCCTTTCACCATTTTCTACAACCCGAGGGCTGCTCCTGCTGAGTTTGTGGTTCCTTTAGCCAAGTATACCAA AGCGATGTACGCTCAAGTTTCCCTCGGTATGCGGTTTAGAATGATATTTGAGACTGAAGAATGTGGAGTTCGTCGGTATATGGGTACAGTTACCGGTATC AGTGATCTTGATCCAGTGAGATGGAAAAACTCTCAGTGGCGGAATCTTCAGATTGGATGGGATGAGTCAGCTGCTGGTGATAGGCCCAGTCGAGTTTCAG TTTGGGACATTGAACCGGTTTTAACTCCTTTCTACATATGTCCTCCTCCATTTTTCCGACCTCGCTTTTCTGGACAACCTGGAATGCCAGATGATGAGAC TGACATGGAGTCTGCACTGAAGAGAGCAATGCCATGGCTTGATAATAGCTTAGAGATGAAAGACCCTTCGAGTACTATCTTTCCTGGTCTGAGTTTAGTT CAGTGGATGAATATGCAGCAGCAGAACGGCCAGCTACCCTCTGCTGCTGCACAGCCAGGTTTCTTCCCATCAATGCTTTCGCCAACCGCGGCGCTGCACA ACAATCTTGGCGGCACTGATGATCCCTCCAAGTTACTGAGCTTTCAGACGCCGCACGGGGGGATTTCCTCCTCAAATCTCCAATTTAACAAACAGAATCA GCAAGCCCCAATGTCTCAGTTGCCTCAGCCACCAACTACGTTGTCCCAACAACAGCAGCTGCAGCAATTGTTGCACTCCTCTTTGAACCATCAACAACAG CAATCGCAGTCTCAACAACAGCAACAACAACAACAGTTGCTGCAGCAGCAACAACAATTGCAGTCTCAACAACACAGCAACAACAATCAATCGCAGTCTC AGCAACAACAACAATTGCTCCAGCAGCAACAACAACAACAACTGCAGCAACAACATCAACAACCGTTACAGCAACAGACTCAGCAGCAGCAGCTAAGAAC ACAGCCATTGCAATCTCACTCGCATCCACAGCCACAACAGTTACAACAACATAAGTTGCAGCAACTTCAGGTTCCACAGAATCAGCTTTACAATGGTCAA CAAGCAGCGCAGCAGCATCAGTCGCAACAAGCATCTACACATCATTTGCAACCACAATTAGTTTCGGGATCAATGGCAAGCAGTGTCATCACGCCTCCGT CCAGCTCCCTTAATCAAAGCTTTCAACAGCAACAACAACAGTCTAAGCAACTTCAACAAGCACATCACCATTTAGGTGCTAGCACTAGCCAGAGTAGTGT AATTGAAACCAGCAAGTCTTCATCCAATCTGATGTCCGCACCGCCGCAAGAGACACAGTTTTCACGACAAGTAGAACAGCAGCAGCCTCCTGGTCTCAAC GGGCAGAATCAGCAAACACTTTTGCAGCAGAAAGCTCACCAGGCACAGGCCCAACAGATATTCCAGCAGAGTCTCTTGGAACAGCCGCATATACAGTTTC AGCTGTTACAGAGATTACAACAGCAACAGCAGCAGCAATTTCTTTCGCCGCAGTCTCAGTTACCACACCATCAATTGCAAAGCCAGCAGTTGCAACAGCT GCCTACTCTCTCTCAAGGTCATCAGTTTCCGTCATCTTGCACTAACAATGGCTTATCGACGTTGCAACCACCTCAAATGCTGGTGAGCCGACCTCAGGAA AAACAAAACCCACCGGTTGGGGGAGGGGTCAAAGCTTATTCAGGCATCACAGATGGAGGAGATGCACCTTCCTCTTCAACGTCGCCTTCCACCAACAACT GTCAGATCTCTTCTTCAGGCTTTCTCAACAGAAGCCAAAGCGGGCCAGCGATCTTGATACCTGATGCAGCGATTGATATGTCTGGTAATCTTGTTCAGGA TCTTTACAGCAAATCCGATATGCGGCTAAAACAAGAACTCGTGGGTCAGCAAAAGTCCAAAGCTAGTTTAACAGATCATCAACTAGAAGCATCTGCCTCT GGAACTTCTTACGGTTTAGATGGAGGCGAAAACAACAGACAACAAAATTTCTTGGCTCCAACTTTTGGCCTTGACGGTGATTCCAGGAACAGCTTGCTCG GTGGAGCTAATGTTGATAATGGCTTTGTGCCTGACACGCTACTCTCGAGGGGATATGACTCCCAGAAAGATCTTCAGAACATGCTTTCAAACTATGGAGG AGTGACAAATGACATTGGTACAGAGATGTCTACTTCAGCTGTAAGAACTCAATCTTTTGGTGTCCCCAATGTGCCCGCCATTTCGAACGATCTAGCTGTC AACGATGCTGGAGTTCTTGGTGGTGGATTGTGGCCAGCTCAGACTCAGCGAATGCGAACTTATACAAAGGTGCAAAAACGAGGCTCAGTGGGGAGATCAA TAGACGTCAACCGTTACAGAGGTTACGATGAGCTGAGGCATGATCTAGCGCGCATGTTTGGGATCGAAGGACAGCTCGAAGATCCTCAAACATCTGACTG GAAACTTGTTTATGTCGATCATGAAAATGACATCCTCCTCGTCGGCGATGATCCATGGGAAGAATTCGTAAACTGTGTTCAGAGCATTAAGATCCTTTCA TCAGCTGAGGTTCAGCAGATGAGCTTAGACGGGAACTTTGCCGGTGTACCAGTTACTAATCAAGCTTGTAGTGGCGGTGACAGTGGCAATGCTTGGAGAG GTCATTATGATGATAACTCAGCCACTTCGTTTAACCGG >IE_05E10_translated MNRQPNEFFCKTLTASDTSTHGGFSVPRRAAEKIFPALDFSMQPPCQELVAKDIHDNTWTFRHIYRGQPKRHLLTTGWSVFVSTKRLFAGDSVLFIRDGK AQLLLGIRRANRQQPALSSSVISSDSMHIGVLAAAAHANANNSPFTIFYNPRAAPAEFVVPLAKYTKAMYAQVSLGMRFRMIFETEECGVRRYMGTVTGI SDLDPVRWKNSQWRNLQIGWDESAAGDRPSRVSVWDIEPVLTPFYICPPPFFRPRFSGQPGMPDDETDMESALKRAMPWLDNSLEMKDPSSTIFPGLSLV QWMNMQQQNGQLPSAAAQPGFFPSMLSPTAALHNNLGGTDDPSKLLSFQTPHGGISSSNLQFNKQNQQAPMSQLPQPPTTLSQQQQLQQLLHSSLNHQQQ QSQSQQQQQQQQLLQQQQQLQSQQHSNNNQSQSQQQQQLLQQQQQQQLQQQHQQPLQQQTQQQQLRTQPLQSHSHPQPQQLQQHKLQQLQVPQNQLYNGQ QAAQQHQSQQASTHHLQPQLVSGSMASSVITPPSSSLNQSFQQQQQQSKQLQQAHHHLGASTSQSSVIETSKSSSNLMSAPPQETQFSRQVEQQQPPGLN GQNQQTLLQQKAHQAQAQQIFQQSLLEQPHIQFQLLQRLQQQQQQQFLSPQSQLPHHQLQSQQLQQLPTLSQGHQFPSSCTNNGLSTLQPPQMLVSRPQE KQNPPVGGGVKAYSGITDGGDAPSSSTSPSTNNCQISSSGFLNRSQSGPAILIPDAAIDMSGNLVQDLYSKSDMRLKQELVGQQKSKASLTDHQLEASAS GTSYGLDGGENNRQQNFLAPTFGLDGDSRNSLLGGANVDNGFVPDTLLSRGYDSQKDLQNMLSNYGGVTNDIGTEMSTSAVRTQSFGVPNVPAISNDLAV NDAGVLGGGLWPAQTQRMRTYTKVQKRGSVGRSIDVNRYRGYDELRHDLARMFGIEGQLEDPQTSDWKLVYVDHENDILLVGDDPWEEFVNCVQSIKILS SAEVQQMSLDGNFAGVPVTNQACSGGDSGNAWRGHYDDNSATSFNR
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 3138/3495 (89.8%)（アミノ酸配列で1045/1165 (89.7%)）一致しました。 10 20 30 40 50 60 70 80 90 100 AT5G20 ATGAAAGCTCCTTCATCAAATGGAGTTTCTCCTAATCCTGTTGAAGGAGAAAGGAGAAATATAAACTCAGAGCTATGGCACGCTTGTGCTGGGCCATTGA IE_05E ---------------------------------------------------------------------------------------------------- 110 120 130 140 150 160 170 180 190 200 AT5G20 TTTCGTTGCCTCCAGCAGGAAGTCTTGTTGTTTACTTCCCTCAAGGTCACAGTGAGCAAGTCGCGGCTTCAATGCAGAAGCAGACTGATTTCATACCAAG IE_05E ---------------------------------------------------------------------------------------------------- 210 220 230 240 250 260 270 280 290 300 AT5G20 TTACCCGAATCTTCCTTCCAAGCTCATATGCATGCTCCACAATGTTACACTGAATGCTGATCCTGAGACGGATGAGGTCTATGCGCAGATGACTCTTCAG IE_05E ---------------------------------------------------------------------------------------------------- 310 320 330 340 350 360 370 380 390 400 AT5G20 CCAGTAAACAAATATGACAGAGATGCATTGCTTGCTTCTGACATGGGTCTTAAGCTAAACAGACAACCTAATGAATTTTTCTGCAAAACCCTCACGGCGA ::: ::::::::::::::::::::::::::::::::::::::::::: IE_05E ------------------------------------------ATG------------AACAGACAACCTAATGAATTTTTCTGCAAAACCCTCACGGCGA 10 20 30 40 410 420 430 440 450 460 470 480 490 500 AT5G20 GTGACACAAGTACTCACGGTGGATTTTCTGTACCCCGACGAGCTGCTGAGAAAATCTTTCCTGCTCTGGATTTCTCGATGCAACCACCTTGTCAGGAGCT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05E GTGACACAAGTACTCACGGTGGATTTTCTGTACCCCGACGAGCTGCTGAGAAAATCTTTCCTGCTCTGGATTTCTCGATGCAACCACCTTGTCAGGAGCT 50 60 70 80 90 100 110 120 130 140 510 520 530 540 550 560 570 580 590 600 AT5G20 TGTTGCTAAGGATATTCATGACAACACATGGACTTTCAGACATATTTATCGAGGTCAACCAAAAAGGCACTTGCTAACTACAGGCTGGAGTGTGTTTGTC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05E TGTTGCTAAGGATATTCATGACAACACATGGACTTTCAGACATATTTATCGAGGTCAACCAAAAAGGCACTTGCTAACTACAGGCTGGAGTGTGTTTGTC 150 160 170 180 190 200 210 220 230 240 610 620 630 640 650 660 670 680 690 700 AT5G20 AGCACGAAAAGGCTCTTTGCTGGAGACTCTGTTCTTTTTATAAGAGATGGAAAGGCGCAACTTCTGTTGGGGATAAGACGTGCAAATAGACAACAGCCTG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05E AGCACGAAAAGGCTCTTTGCTGGAGACTCTGTTCTTTTTATAAGAGATGGAAAGGCGCAACTTCTGTTGGGGATAAGACGTGCAAATAGACAACAGCCTG 250 260 270 280 290 300 310 320 330 340 710 720 730 740 750 760 770 780 790 800 AT5G20 CACTTTCTTCATCTGTAATATCAAGTGATAGCATGCACATCGGAGTTCTTGCAGCTGCAGCTCATGCTAATGCTAATAACAGTCCTTTCACCATTTTCTA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05E CACTTTCTTCATCTGTAATATCAAGTGATAGCATGCACATCGGAGTTCTTGCAGCTGCAGCTCATGCTAATGCTAATAACAGTCCTTTCACCATTTTCTA 350 360 370 380 390 400 410 420 430 440 810 820 830 840 850 860 870 880 890 900 AT5G20 CAACCCGAGGTGGGCTGCTCCTGCTGAGTTTGTGGTTCCTTTAGCCAAGTATACCAAAGCGATGTACGCTCAAGTTTCCCTCGGTATGCGGTTTAGAATG :::::::: ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05E CAACCCGA---GGGCTGCTCCTGCTGAGTTTGTGGTTCCTTTAGCCAAGTATACCAAAGCGATGTACGCTCAAGTTTCCCTCGGTATGCGGTTTAGAATG 450 460 470 480 490 500 510 520 530 540 910 920 930 940 950 960 970 980 990 1000 AT5G20 ATATTTGAGACTGAAGAATGTGGAGTTCGTCGGTATATGGGTACAGTTACCGGTATCAGTGATCTTGATCCAGTGAGATGGAAAAACTCTCAGTGGCGGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05E ATATTTGAGACTGAAGAATGTGGAGTTCGTCGGTATATGGGTACAGTTACCGGTATCAGTGATCTTGATCCAGTGAGATGGAAAAACTCTCAGTGGCGGA 550 560 570 580 590 600 610 620 630 640 1010 1020 1030 1040 1050 1060 1070 1080 1090 1100 AT5G20 ATCTTCAGATTGGATGGGATGAGTCAGCTGCTGGTGATAGGCCCAGTCGAGTTTCAGTTTGGGACATTGAACCGGTTTTAACTCCTTTCTACATATGTCC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05E ATCTTCAGATTGGATGGGATGAGTCAGCTGCTGGTGATAGGCCCAGTCGAGTTTCAGTTTGGGACATTGAACCGGTTTTAACTCCTTTCTACATATGTCC 650 660 670 680 690 700 710 720 730 740 1110 1120 1130 1140 1150 1160 1170 1180 1190 1200 AT5G20 TCCTCCATTTTTCCGACCTCGCTTTTCTGGACAACCTGGAATGCCAGATGATGAGACTGACATGGAGTCTGCACTGAAGAGAGCAATGCCATGGCTTGAT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05E TCCTCCATTTTTCCGACCTCGCTTTTCTGGACAACCTGGAATGCCAGATGATGAGACTGACATGGAGTCTGCACTGAAGAGAGCAATGCCATGGCTTGAT 750 760 770 780 790 800 810 820 830 840 1210 1220 1230 1240 1250 1260 1270 1280 1290 1300 AT5G20 AATAGCTTAGAGATGAAAGACCCTTCGAGTACTATCTTTCCTGGTCTGAGTTTAGTTCAGTGGATGAATATGCAGCAGCAGAACGGCCAGCTACCCTCTG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05E AATAGCTTAGAGATGAAAGACCCTTCGAGTACTATCTTTCCTGGTCTGAGTTTAGTTCAGTGGATGAATATGCAGCAGCAGAACGGCCAGCTACCCTCTG 850 860 870 880 890 900 910 920 930 940 1310 1320 1330 1340 1350 1360 1370 1380 1390 1400 AT5G20 CTGCTGCACAGCCAGGTTTCTTCCCATCAATGCTTTCGCCAACCGCGGCGCTGCACAACAATCTTGGCGGCACTGATGATCCCTCCAAGTTACTGAGCTT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05E CTGCTGCACAGCCAGGTTTCTTCCCATCAATGCTTTCGCCAACCGCGGCGCTGCACAACAATCTTGGCGGCACTGATGATCCCTCCAAGTTACTGAGCTT 950 960 970 980 990 1000 1010 1020 1030 1040 1410 1420 1430 1440 1450 1460 1470 1480 1490 1500 AT5G20 TCAGACGCCGCACGGGGGGATTTCCTCCTCAAATCTCCAATTTAACAAACAGAATCAGCAAGCCCCAATGTCTCAGTTGCCTCAGCCACCAACTACGTTG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05E TCAGACGCCGCACGGGGGGATTTCCTCCTCAAATCTCCAATTTAACAAACAGAATCAGCAAGCCCCAATGTCTCAGTTGCCTCAGCCACCAACTACGTTG 1050 1060 1070 1080 1090 1100 1110 1120 1130 1140 1510 1520 1530 1540 1550 1560 1570 1580 1590 1600 AT5G20 TCCCAACAACAGCAGCTGCAGCAATTGTTGCACTCCTCTTTGAACCATCAACAACAGCAATCGCAGTCTCAACAACAGCAACAACAACAACAGTTGCTGC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05E TCCCAACAACAGCAGCTGCAGCAATTGTTGCACTCCTCTTTGAACCATCAACAACAGCAATCGCAGTCTCAACAACAGCAACAACAACAACAGTTGCTGC 1150 1160 1170 1180 1190 1200 1210 1220 1230 1240 1610 1620 1630 1640 1650 1660 1670 1680 1690 1700 AT5G20 AGCAGCAACAACAATTGCAGTCTCAACAACACAGCAACAACAATCAATCGCAGTCTCAGCAACAACAACAATTGCTCCAGCAGCAACAACAACAACAACT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05E AGCAGCAACAACAATTGCAGTCTCAACAACACAGCAACAACAATCAATCGCAGTCTCAGCAACAACAACAATTGCTCCAGCAGCAACAACAACAACAACT 1250 1260 1270 1280 1290 1300 1310 1320 1330 1340 1710 1720 1730 1740 1750 1760 1770 1780 1790 1800 AT5G20 GCAGCAACAACATCAACAACCGTTACAGCAACAGACTCAGCAGCAGCAGCTAAGAACACAGCCATTGCAATCTCACTCGCATCCACAGCCACAACAGTTA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05E GCAGCAACAACATCAACAACCGTTACAGCAACAGACTCAGCAGCAGCAGCTAAGAACACAGCCATTGCAATCTCACTCGCATCCACAGCCACAACAGTTA 1350 1360 1370 1380 1390 1400 1410 1420 1430 1440 1810 1820 1830 1840 1850 1860 1870 1880 1890 1900 AT5G20 CAACAACATAAGTTGCAGCAACTTCAGGTTCCACAGAATCAGCTTTACAATGGTCAACAAGCAGCGCAGCAGCATCAGTCGCAACAAGCATCTACACATC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05E CAACAACATAAGTTGCAGCAACTTCAGGTTCCACAGAATCAGCTTTACAATGGTCAACAAGCAGCGCAGCAGCATCAGTCGCAACAAGCATCTACACATC 1450 1460 1470 1480 1490 1500 1510 1520 1530 1540 1910 1920 1930 1940 1950 1960 1970 1980 1990 2000 AT5G20 ATTTGCAACCACAATTAGTTTCGGGATCAATGGCAAGCAGTGTCATCACGCCTCCGTCCAGCTCCCTTAATCAAAGCTTTCAACAGCAACAACAACAGTC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05E ATTTGCAACCACAATTAGTTTCGGGATCAATGGCAAGCAGTGTCATCACGCCTCCGTCCAGCTCCCTTAATCAAAGCTTTCAACAGCAACAACAACAGTC 1550 1560 1570 1580 1590 1600 1610 1620 1630 1640 2010 2020 2030 2040 2050 2060 2070 2080 2090 2100 AT5G20 TAAGCAACTTCAACAAGCACATCACCATTTAGGTGCTAGCACTAGCCAGAGTAGTGTAATTGAAACCAGCAAGTCTTCATCCAATCTGATGTCCGCACCG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05E TAAGCAACTTCAACAAGCACATCACCATTTAGGTGCTAGCACTAGCCAGAGTAGTGTAATTGAAACCAGCAAGTCTTCATCCAATCTGATGTCCGCACCG 1650 1660 1670 1680 1690 1700 1710 1720 1730 1740 2110 2120 2130 2140 2150 2160 2170 2180 2190 2200 AT5G20 CCGCAAGAGACACAGTTTTCACGACAAGTAGAACAGCAGCAGCCTCCTGGTCTCAACGGGCAGAATCAGCAAACACTTTTGCAGCAGAAAGCTCACCAGG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05E CCGCAAGAGACACAGTTTTCACGACAAGTAGAACAGCAGCAGCCTCCTGGTCTCAACGGGCAGAATCAGCAAACACTTTTGCAGCAGAAAGCTCACCAGG 1750 1760 1770 1780 1790 1800 1810 1820 1830 1840 2210 2220 2230 2240 2250 2260 2270 2280 2290 2300 AT5G20 CACAGGCCCAACAGATATTCCAGCAGAGTCTCTTGGAACAGCCGCATATACAGTTTCAGCTGTTACAGAGATTACAACAGCAACAGCAGCAGCAATTTCT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05E CACAGGCCCAACAGATATTCCAGCAGAGTCTCTTGGAACAGCCGCATATACAGTTTCAGCTGTTACAGAGATTACAACAGCAACAGCAGCAGCAATTTCT 1850 1860 1870 1880 1890 1900 1910 1920 1930 1940 2310 2320 2330 2340 2350 2360 2370 2380 2390 2400 AT5G20 TTCGCCGCAGTCTCAGTTACCACACCATCAATTGCAAAGCCAGCAGTTGCAACAGCTGCCTACTCTCTCTCAAGGTCATCAGTTTCCGTCATCTTGCACT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05E TTCGCCGCAGTCTCAGTTACCACACCATCAATTGCAAAGCCAGCAGTTGCAACAGCTGCCTACTCTCTCTCAAGGTCATCAGTTTCCGTCATCTTGCACT 1950 1960 1970 1980 1990 2000 2010 2020 2030 2040 2410 2420 2430 2440 2450 2460 2470 2480 2490 2500 AT5G20 AACAATGGCTTATCGACGTTGCAACCACCTCAAATGCTGGTGAGCCGACCTCAGGAAAAACAAAACCCACCGGTTGGGGGAGGGGTCAAAGCTTATTCAG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05E AACAATGGCTTATCGACGTTGCAACCACCTCAAATGCTGGTGAGCCGACCTCAGGAAAAACAAAACCCACCGGTTGGGGGAGGGGTCAAAGCTTATTCAG 2050 2060 2070 2080 2090 2100 2110 2120 2130 2140 2510 2520 2530 2540 2550 2560 2570 2580 2590 2600 AT5G20 GCATCACAGATGGAGGAGATGCACCTTCCTCTTCAACGTCGCCTTCCACCAACAACTGTCAGATCTCTTCTTCAGGCTTTCTCAACAGAAGCCAAAGCGG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05E GCATCACAGATGGAGGAGATGCACCTTCCTCTTCAACGTCGCCTTCCACCAACAACTGTCAGATCTCTTCTTCAGGCTTTCTCAACAGAAGCCAAAGCGG 2150 2160 2170 2180 2190 2200 2210 2220 2230 2240 2610 2620 2630 2640 2650 2660 2670 2680 2690 2700 AT5G20 GCCAGCGATCTTGATACCTGATGCAGCGATTGATATGTCTGGTAATCTTGTTCAGGATCTTTACAGCAAATCCGATATGCGGCTAAAACAAGAACTCGTG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05E GCCAGCGATCTTGATACCTGATGCAGCGATTGATATGTCTGGTAATCTTGTTCAGGATCTTTACAGCAAATCCGATATGCGGCTAAAACAAGAACTCGTG 2250 2260 2270 2280 2290 2300 2310 2320 2330 2340 2710 2720 2730 2740 2750 2760 2770 2780 2790 2800 AT5G20 GGTCAGCAAAAGTCCAAAGCTAGTTTAACAGATCATCAACTAGAAGCATCTGCCTCTGGAACTTCTTACGGTTTAGATGGAGGCGAAAACAACAGACAAC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05E GGTCAGCAAAAGTCCAAAGCTAGTTTAACAGATCATCAACTAGAAGCATCTGCCTCTGGAACTTCTTACGGTTTAGATGGAGGCGAAAACAACAGACAAC 2350 2360 2370 2380 2390 2400 2410 2420 2430 2440 2810 2820 2830 2840 2850 2860 2870 2880 2890 2900 AT5G20 AAAATTTCTTGGCTCCAACTTTTGGCCTTGACGGTGATTCCAGGAACAGCTTGCTCGGTGGAGCTAATGTTGATAATGGCTTTGTGCCTGACACGCTACT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05E AAAATTTCTTGGCTCCAACTTTTGGCCTTGACGGTGATTCCAGGAACAGCTTGCTCGGTGGAGCTAATGTTGATAATGGCTTTGTGCCTGACACGCTACT 2450 2460 2470 2480 2490 2500 2510 2520 2530 2540 2910 2920 2930 2940 2950 2960 2970 2980 2990 3000 AT5G20 CTCGAGGGGATATGACTCCCAGAAAGATCTTCAGAACATGCTTTCAAACTATGGAGGAGTGACAAATGACATTGGTACAGAGATGTCTACTTCAGCTGTA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05E CTCGAGGGGATATGACTCCCAGAAAGATCTTCAGAACATGCTTTCAAACTATGGAGGAGTGACAAATGACATTGGTACAGAGATGTCTACTTCAGCTGTA 2550 2560 2570 2580 2590 2600 2610 2620 2630 2640 3010 3020 3030 3040 3050 3060 3070 3080 3090 3100 AT5G20 AGAACTCAATCTTTTGGTGTCCCCAATGTGCCCGCCATTTCGAACGATCTAGCTGTCAACGATGCTGGAGTTCTTGGTGGTGGATTGTGGCCAGCTCAGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05E AGAACTCAATCTTTTGGTGTCCCCAATGTGCCCGCCATTTCGAACGATCTAGCTGTCAACGATGCTGGAGTTCTTGGTGGTGGATTGTGGCCAGCTCAGA 2650 2660 2670 2680 2690 2700 2710 2720 2730 2740 3110 3120 3130 3140 3150 3160 3170 3180 3190 3200 AT5G20 CTCAGCGAATGCGAACTTATACAAAGGTGCAAAAACGAGGCTCAGTGGGGAGATCAATAGACGTCAACCGTTACAGAGGTTACGATGAGCTGAGGCATGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05E CTCAGCGAATGCGAACTTATACAAAGGTGCAAAAACGAGGCTCAGTGGGGAGATCAATAGACGTCAACCGTTACAGAGGTTACGATGAGCTGAGGCATGA 2750 2760 2770 2780 2790 2800 2810 2820 2830 2840 3210 3220 3230 3240 3250 3260 3270 3280 3290 3300 AT5G20 TCTAGCGCGCATGTTTGGGATCGAAGGACAGCTCGAAGATCCTCAAACATCTGACTGGAAACTTGTTTATGTCGATCATGAAAATGACATCCTCCTCGTC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05E TCTAGCGCGCATGTTTGGGATCGAAGGACAGCTCGAAGATCCTCAAACATCTGACTGGAAACTTGTTTATGTCGATCATGAAAATGACATCCTCCTCGTC 2850 2860 2870 2880 2890 2900 2910 2920 2930 2940 3310 3320 3330 3340 3350 3360 3370 3380 3390 3400 AT5G20 GGCGATGATCCATGGGAAGAATTCGTAAACTGTGTTCAGAGCATTAAGATCCTTTCATCAGCTGAGGTTCAGCAGATGAGCTTAGACGGGAACTTTGCCG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05E GGCGATGATCCATGGGAAGAATTCGTAAACTGTGTTCAGAGCATTAAGATCCTTTCATCAGCTGAGGTTCAGCAGATGAGCTTAGACGGGAACTTTGCCG 2950 2960 2970 2980 2990 3000 3010 3020 3030 3040 3410 3420 3430 3440 3450 3460 3470 3480 3490 AT5G20 GTGTACCAGTTACTAATCAAGCTTGTAGTGGCGGTGACAGTGGCAATGCTTGGAGAGGTCATTATGATGATAACTCAGCCACTTCGTTTAACCGG ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05E GTGTACCAGTTACTAATCAAGCTTGTAGTGGCGGTGACAGTGGCAATGCTTGGAGAGGTCATTATGATGATAACTCAGCCACTTCGTTTAACCGG 3050 3060 3070 3080 3090 3100 3110 3120 3130 10 20 30 40 50 60 70 80 90 100 AT5G20 MKAPSSNGVSPNPVEGERRNINSELWHACAGPLISLPPAGSLVVYFPQGHSEQVAASMQKQTDFIPSYPNLPSKLICMLHNVTLNADPETDEVYAQMTLQ IE_05E ---------------------------------------------------------------------------------------------------- 110 120 130 140 150 160 170 180 190 200 AT5G20 PVNKYDRDALLASDMGLKLNRQPNEFFCKTLTASDTSTHGGFSVPRRAAEKIFPALDFSMQPPCQELVAKDIHDNTWTFRHIYRGQPKRHLLTTGWSVFV .::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05E ------------------MNRQPNEFFCKTLTASDTSTHGGFSVPRRAAEKIFPALDFSMQPPCQELVAKDIHDNTWTFRHIYRGQPKRHLLTTGWSVFV 10 20 30 40 50 60 70 80 210 220 230 240 250 260 270 280 290 300 AT5G20 STKRLFAGDSVLFIRDGKAQLLLGIRRANRQQPALSSSVISSDSMHIGVLAAAAHANANNSPFTIFYNPRWAAPAEFVVPLAKYTKAMYAQVSLGMRFRM :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: ::::::::::::::::::::::::::::: IE_05E STKRLFAGDSVLFIRDGKAQLLLGIRRANRQQPALSSSVISSDSMHIGVLAAAAHANANNSPFTIFYNPR-AAPAEFVVPLAKYTKAMYAQVSLGMRFRM 90 100 110 120 130 140 150 160 170 180 310 320 330 340 350 360 370 380 390 400 AT5G20 IFETEECGVRRYMGTVTGISDLDPVRWKNSQWRNLQIGWDESAAGDRPSRVSVWDIEPVLTPFYICPPPFFRPRFSGQPGMPDDETDMESALKRAMPWLD :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05E IFETEECGVRRYMGTVTGISDLDPVRWKNSQWRNLQIGWDESAAGDRPSRVSVWDIEPVLTPFYICPPPFFRPRFSGQPGMPDDETDMESALKRAMPWLD 190 200 210 220 230 240 250 260 270 280 410 420 430 440 450 460 470 480 490 500 AT5G20 NSLEMKDPSSTIFPGLSLVQWMNMQQQNGQLPSAAAQPGFFPSMLSPTAALHNNLGGTDDPSKLLSFQTPHGGISSSNLQFNKQNQQAPMSQLPQPPTTL :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05E NSLEMKDPSSTIFPGLSLVQWMNMQQQNGQLPSAAAQPGFFPSMLSPTAALHNNLGGTDDPSKLLSFQTPHGGISSSNLQFNKQNQQAPMSQLPQPPTTL 290 300 310 320 330 340 350 360 370 380 510 520 530 540 550 560 570 580 590 600 AT5G20 SQQQQLQQLLHSSLNHQQQQSQSQQQQQQQQLLQQQQQLQSQQHSNNNQSQSQQQQQLLQQQQQQQLQQQHQQPLQQQTQQQQLRTQPLQSHSHPQPQQL :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05E SQQQQLQQLLHSSLNHQQQQSQSQQQQQQQQLLQQQQQLQSQQHSNNNQSQSQQQQQLLQQQQQQQLQQQHQQPLQQQTQQQQLRTQPLQSHSHPQPQQL 390 400 410 420 430 440 450 460 470 480 610 620 630 640 650 660 670 680 690 700 AT5G20 QQHKLQQLQVPQNQLYNGQQAAQQHQSQQASTHHLQPQLVSGSMASSVITPPSSSLNQSFQQQQQQSKQLQQAHHHLGASTSQSSVIETSKSSSNLMSAP :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05E QQHKLQQLQVPQNQLYNGQQAAQQHQSQQASTHHLQPQLVSGSMASSVITPPSSSLNQSFQQQQQQSKQLQQAHHHLGASTSQSSVIETSKSSSNLMSAP 490 500 510 520 530 540 550 560 570 580 710 720 730 740 750 760 770 780 790 800 AT5G20 PQETQFSRQVEQQQPPGLNGQNQQTLLQQKAHQAQAQQIFQQSLLEQPHIQFQLLQRLQQQQQQQFLSPQSQLPHHQLQSQQLQQLPTLSQGHQFPSSCT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05E PQETQFSRQVEQQQPPGLNGQNQQTLLQQKAHQAQAQQIFQQSLLEQPHIQFQLLQRLQQQQQQQFLSPQSQLPHHQLQSQQLQQLPTLSQGHQFPSSCT 590 600 610 620 630 640 650 660 670 680 810 820 830 840 850 860 870 880 890 900 AT5G20 NNGLSTLQPPQMLVSRPQEKQNPPVGGGVKAYSGITDGGDAPSSSTSPSTNNCQISSSGFLNRSQSGPAILIPDAAIDMSGNLVQDLYSKSDMRLKQELV :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05E NNGLSTLQPPQMLVSRPQEKQNPPVGGGVKAYSGITDGGDAPSSSTSPSTNNCQISSSGFLNRSQSGPAILIPDAAIDMSGNLVQDLYSKSDMRLKQELV 690 700 710 720 730 740 750 760 770 780 910 920 930 940 950 960 970 980 990 1000 AT5G20 GQQKSKASLTDHQLEASASGTSYGLDGGENNRQQNFLAPTFGLDGDSRNSLLGGANVDNGFVPDTLLSRGYDSQKDLQNMLSNYGGVTNDIGTEMSTSAV :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05E GQQKSKASLTDHQLEASASGTSYGLDGGENNRQQNFLAPTFGLDGDSRNSLLGGANVDNGFVPDTLLSRGYDSQKDLQNMLSNYGGVTNDIGTEMSTSAV 790 800 810 820 830 840 850 860 870 880 1010 1020 1030 1040 1050 1060 1070 1080 1090 1100 AT5G20 RTQSFGVPNVPAISNDLAVNDAGVLGGGLWPAQTQRMRTYTKVQKRGSVGRSIDVNRYRGYDELRHDLARMFGIEGQLEDPQTSDWKLVYVDHENDILLV :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05E RTQSFGVPNVPAISNDLAVNDAGVLGGGLWPAQTQRMRTYTKVQKRGSVGRSIDVNRYRGYDELRHDLARMFGIEGQLEDPQTSDWKLVYVDHENDILLV 890 900 910 920 930 940 950 960 970 980 1110 1120 1130 1140 1150 1160 AT5G20 GDDPWEEFVNCVQSIKILSSAEVQQMSLDGNFAGVPVTNQACSGGDSGNAWRGHYDDNSATSFNR ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05E GDDPWEEFVNCVQSIKILSSAEVQQMSLDGNFAGVPVTNQACSGGDSGNAWRGHYDDNSATSFNR 990 1000 1010 1020 1030 1040

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