◆◆◆IE_05G02 detail◆◆◆

Code name	IE_05G02
Locus	AT4G31615
Template	IT_05G02 (HR3301)
Donor Vector	pDONR207
Forward primer	IPF_05G02 (ggggacaagtttgtacaaaaaagcaggcttcATGGATGATCCAGCAATTTCCTCTCCAA)
Reverse primer	IPR_05G02 (ggggaccactttgtacaagaaagctgggttGATCATACCTAAAGCATCAATTTCCAGTAT)
Alignment with template seq.	1st	アミノ酸配列100%一致	2nd	-
Alignment with TAIR7CDS	1st	アミノ酸配列100%一致	2nd	-
comment
sequence	>IE_05G02_1434bp ATGGATGATCCAGCAATTTCCTCTCCAACCAACAAGCATTTCTTCGTCATAGTTCTTCATGGACACAAATCCAGTCCCATGATTCCTGCTGAGTTTTTTT CGACTTACGTGGAAGGTAAAAACCACCAGTCTACAAAACTGAAACTGACCTCGGACGCTTTCGATAGAACCTGGGAAGTGAAATTGAACGGCCGCAGATT CGCCGGCGGCTGGGAAAATTTCTCCACCGTACACTCTCTCCAAGATGACGACGTTGTGATTTTCAGGGAAATCGGGGACATGACCTTTCACGTCACAGCC TCCGGTCGCAGTTTCTGTAAGTTACAGTACATATCCTCTGACGAAGATAGGGCTGATGAAGATGATGCATGGAACGTGTATTCAGATTCCAAGAACATTG TGTCGAAGAAGAAGCCAAGAACAGAAGCAGAGTCTTCATCCAAAAACTATTATCTTGTTGCGCATGTCACGCCTTCGAACCTAAGCCGAGATAGATTGTA TCTTATAAAGGCTTTTGCGAGGTCAAATGGCCTGAACGAGAGATGCTGTGAGATTGATCTAATGAATAAACATGGAAAATCATGGACACTTGGTCTGAGA TACAGTACAGTCAATGATCAGGCTAGTATCAGTGGAGGCTGGAGAGACTTCTGCTTAGTAAATGGGCTAGAAGCTGGATCATTCTACAGGTTTAAGCTTG TCCAAAACGGGGAAAGGCCTTTACTACGGTTGTGTTACGACACTATTCCAGAAGAAGACTGTTACAAAGGAAACAGAAAAGTTAATCTTTCAGAGAGTGC TTCAATGAAGCAGGGCAAGTTTCTGACAATGACCTTTAAGCCTTACATGCTCAAGTCAGGTCAACTTCGTCTCTCGAGACCCTTTGCAATAGAGAATGAA ATAAAGGAGGCAGAGGATATAACACTAGTGGACAAAAATGGAGGAAACTGGCCATCCTATGTAGCTTCTGGCGATGGTCAAGGAGGAAAGTATTATTTGG CAAAAGGTTGGAGAAGTTTTTGTGCAGCCAATAGGTTAAAGACGGGAGAGACCTTCACGTTGGAGTTTGTTCGAGGAGAAGGCACAACTCCAATGCTAAA GTTCTGCTCCAAGTCCAAGGACAAGATAGAACAAGTACCATTTGATGAAGGAGGGGAGACTCAAGAAGAAACAGAGACTAGGGTCAAAAAGAGAGCTCGA GTTTCTGCAGAAAGAGGATCTTCTCGCCGCAATCAGGCATCAAATAAACCCAATGCTGATCCACAAAACTTGCAGCCTGGGCAACCACATTGCTCAATCT CTGATCAAGTGCAAAAGGTGAAACAGATTATTGTTGACACTTTAACCGGGATAAAACGGATTCGGTCAGAGCTAGAAAGAAAGGGACAAAAGTTAGAAGC TTCGATACTGGAAATTGATGCTTTAGGTATGATC >IE_05G02_translated MDDPAISSPTNKHFFVIVLHGHKSSPMIPAEFFSTYVEGKNHQSTKLKLTSDAFDRTWEVKLNGRRFAGGWENFSTVHSLQDDDVVIFREIGDMTFHVTA SGRSFCKLQYISSDEDRADEDDAWNVYSDSKNIVSKKKPRTEAESSSKNYYLVAHVTPSNLSRDRLYLIKAFARSNGLNERCCEIDLMNKHGKSWTLGLR YSTVNDQASISGGWRDFCLVNGLEAGSFYRFKLVQNGERPLLRLCYDTIPEEDCYKGNRKVNLSESASMKQGKFLTMTFKPYMLKSGQLRLSRPFAIENE IKEAEDITLVDKNGGNWPSYVASGDGQGGKYYLAKGWRSFCAANRLKTGETFTLEFVRGEGTTPMLKFCSKSKDKIEQVPFDEGGETQEETETRVKKRAR VSAERGSSRRNQASNKPNADPQNLQPGQPHCSISDQVQKVKQIIVDTLTGIKRIRSELERKGQKLEASILEIDALGMI
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 1431/1461 (97.9%)（アミノ酸配列で476/487 (97.7%)）一致しました。 10 20 30 40 50 60 70 80 90 100 AT4G31 ATGGATGATCCAGCAATTTCCTCTCCAACCAACAAGCATTTCTTCGTCATAGTTCTTCATGGACACAAATCCAGTCCCATGATTCCTGCTGAGTTTTTTT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05G ATGGATGATCCAGCAATTTCCTCTCCAACCAACAAGCATTTCTTCGTCATAGTTCTTCATGGACACAAATCCAGTCCCATGATTCCTGCTGAGTTTTTTT 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT4G31 CGACTTACGTGGAAGGTAAAAACCACCAGTCTACAAAACTGAAACTGACCTCGGACGCTTTCGATAGAACCTGGGAAGTGAAATTGAACGGCCGCAGATT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05G CGACTTACGTGGAAGGTAAAAACCACCAGTCTACAAAACTGAAACTGACCTCGGACGCTTTCGATAGAACCTGGGAAGTGAAATTGAACGGCCGCAGATT 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT4G31 CGCCGGCGGCTGGGAAAATTTCTCCACCGTACACTCTCTCCAAGATGACGACGTTGTGATTTTCAGGGAAATCGGGGACATGACCTTTCACGTCACAGCC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05G CGCCGGCGGCTGGGAAAATTTCTCCACCGTACACTCTCTCCAAGATGACGACGTTGTGATTTTCAGGGAAATCGGGGACATGACCTTTCACGTCACAGCC 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 AT4G31 TCCGGTCGCAGTTTCTGTAAGTTACAGTACATATCCTCTGACGAAGATAGGGCTGATGAAGATGATGCATGGAACGTGTATTCAGATTCCAAGAACATTG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05G TCCGGTCGCAGTTTCTGTAAGTTACAGTACATATCCTCTGACGAAGATAGGGCTGATGAAGATGATGCATGGAACGTGTATTCAGATTCCAAGAACATTG 310 320 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 480 490 500 AT4G31 TGTCGAAGAAGAAGCCAAGAACAGAAGCAGAGTCTTCATCCAAAAACTATTATCTTGTTGCGCATGTCACGCCTTCGAACCTAAGCCGAGATAGATTGTA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05G TGTCGAAGAAGAAGCCAAGAACAGAAGCAGAGTCTTCATCCAAAAACTATTATCTTGTTGCGCATGTCACGCCTTCGAACCTAAGCCGAGATAGATTGTA 410 420 430 440 450 460 470 480 490 500 510 520 530 540 550 560 570 580 590 600 AT4G31 TCTTATAAAGGCTTTTGCGAGGTCAAATGGCCTGAACGAGAGATGCTGTGAGATTGATCTAATGAATAAACATGGAAAATCATGGACACTTGGTCTGAGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05G TCTTATAAAGGCTTTTGCGAGGTCAAATGGCCTGAACGAGAGATGCTGTGAGATTGATCTAATGAATAAACATGGAAAATCATGGACACTTGGTCTGAGA 510 520 530 540 550 560 570 580 590 600 610 620 630 640 650 660 670 680 690 700 AT4G31 TACAGTACAGTCAATGATCAGGCTAGTATCAGTGGAGGCTGGAGAGACTTCTGCTTAGTAAATGGGCTAGAAGCTGGATCATTCTACAGGTTTAAGCTTG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05G TACAGTACAGTCAATGATCAGGCTAGTATCAGTGGAGGCTGGAGAGACTTCTGCTTAGTAAATGGGCTAGAAGCTGGATCATTCTACAGGTTTAAGCTTG 610 620 630 640 650 660 670 680 690 700 710 720 730 740 750 760 770 780 790 800 AT4G31 TCCAAAACGGGGAAAGGCCTTTACTACGGTTGTGTTACGACACTATTCCAGAAGAAGACTGTTACAAAGGAAACAGAAAAGTTAATCTTTCAGAGAGTGC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05G TCCAAAACGGGGAAAGGCCTTTACTACGGTTGTGTTACGACACTATTCCAGAAGAAGACTGTTACAAAGGAAACAGAAAAGTTAATCTTTCAGAGAGTGC 710 720 730 740 750 760 770 780 790 800 810 820 830 840 850 860 870 880 890 900 AT4G31 TTCAATGAAGCAGGGCAAGTTTCTGACAATGACCTTTAAGCCTTACATGCTCAAGTCAGGTCAACTTCGTCTCTCGAGACCCTTTGCAATAGAGAATGAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05G TTCAATGAAGCAGGGCAAGTTTCTGACAATGACCTTTAAGCCTTACATGCTCAAGTCAGGTCAACTTCGTCTCTCGAGACCCTTTGCAATAGAGAATGAA 810 820 830 840 850 860 870 880 890 900 910 920 930 940 950 960 970 980 990 1000 AT4G31 ATAAAGGAGGCAGAGGATATAACACTAGTGGACAAAAATGGAGGAAACTGGCCATCCTATGTAGCTTCTGGCGATGGTCAAGGAGGAAAGTATTATTTGG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05G ATAAAGGAGGCAGAGGATATAACACTAGTGGACAAAAATGGAGGAAACTGGCCATCCTATGTAGCTTCTGGCGATGGTCAAGGAGGAAAGTATTATTTGG 910 920 930 940 950 960 970 980 990 1000 1010 1020 1030 1040 1050 1060 1070 1080 1090 1100 AT4G31 CAAAAGGTTGGAGAAGTTTTTGTGCAGCCAATAGGTTAAAGACGGGAGAGACCTTCACGTTGGAGTTTGTTCGAGGAGAAGGCACAACTCCAATGCTAAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05G CAAAAGGTTGGAGAAGTTTTTGTGCAGCCAATAGGTTAAAGACGGGAGAGACCTTCACGTTGGAGTTTGTTCGAGGAGAAGGCACAACTCCAATGCTAAA 1010 1020 1030 1040 1050 1060 1070 1080 1090 1100 1110 1120 1130 1140 1150 1160 1170 1180 1190 1200 AT4G31 GTTCTGCTCCAAGTCCAAGGACAAGATAGAACAAGTACCATTTGATGAAGGAGGGGAGACTCAAGAAGAAACAGAGACTAGGGTCAAAAAGAGAGCTCGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05G GTTCTGCTCCAAGTCCAAGGACAAGATAGAACAAGTACCATTTGATGAAGGAGGGGAGACTCAAGAAGAAACAGAGACTAGGGTCAAAAAGAGAGCTCGA 1110 1120 1130 1140 1150 1160 1170 1180 1190 1200 1210 1220 1230 1240 1250 1260 1270 1280 1290 1300 AT4G31 GTTTCTGCAGAAAGAGGATCTTCTCGCCGCAATCAGGCATCAAATAAACCCAATGCTGATCCACAAAACTTGCAGCCTGGGCAACCACATTGCTCAATCT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05G GTTTCTGCAGAAAGAGGATCTTCTCGCCGCAATCAGGCATCAAATAAACCCAATGCTGATCCACAAAACTTGCAGCCTGGGCAACCACATTGCTCAATCT 1210 1220 1230 1240 1250 1260 1270 1280 1290 1300 1310 1320 1330 1340 1350 1360 1370 1380 1390 1400 AT4G31 CTGATCAAGTGCAAAAGGTGAAACAGATTATTGTTGACACTTTAACCGGGATAAAACGGATTCGGTCAGAGCTAGAAAGAAAGGGACAAAAGTTAGAAGC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05G CTGATCAAGTGCAAAAGGTGAAACAGATTATTGTTGACACTTTAACCGGGATAAAACGGATTCGGTCAGAGCTAGAAAGAAAGGGACAAAAGTTAGAAGC 1310 1320 1330 1340 1350 1360 1370 1380 1390 1400 1410 1420 1430 1440 1450 1460 AT4G31 TTCGATACTGGAAATTGATGCTTTAGGGGAGAAAGTGTCGGAAATCAACAAAATCCTCAAG ::::::::::::::::::::::::::: :: :: IE_05G TTCGATACTGGAAATTGATGCTTTAGGTATGA-----TC---------------------- 1410 1420 1430 10 20 30 40 50 60 70 80 90 100 AT4G31 MDDPAISSPTNKHFFVIVLHGHKSSPMIPAEFFSTYVEGKNHQSTKLKLTSDAFDRTWEVKLNGRRFAGGWENFSTVHSLQDDDVVIFREIGDMTFHVTA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05G MDDPAISSPTNKHFFVIVLHGHKSSPMIPAEFFSTYVEGKNHQSTKLKLTSDAFDRTWEVKLNGRRFAGGWENFSTVHSLQDDDVVIFREIGDMTFHVTA 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT4G31 SGRSFCKLQYISSDEDRADEDDAWNVYSDSKNIVSKKKPRTEAESSSKNYYLVAHVTPSNLSRDRLYLIKAFARSNGLNERCCEIDLMNKHGKSWTLGLR :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05G SGRSFCKLQYISSDEDRADEDDAWNVYSDSKNIVSKKKPRTEAESSSKNYYLVAHVTPSNLSRDRLYLIKAFARSNGLNERCCEIDLMNKHGKSWTLGLR 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT4G31 YSTVNDQASISGGWRDFCLVNGLEAGSFYRFKLVQNGERPLLRLCYDTIPEEDCYKGNRKVNLSESASMKQGKFLTMTFKPYMLKSGQLRLSRPFAIENE :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05G YSTVNDQASISGGWRDFCLVNGLEAGSFYRFKLVQNGERPLLRLCYDTIPEEDCYKGNRKVNLSESASMKQGKFLTMTFKPYMLKSGQLRLSRPFAIENE 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 AT4G31 IKEAEDITLVDKNGGNWPSYVASGDGQGGKYYLAKGWRSFCAANRLKTGETFTLEFVRGEGTTPMLKFCSKSKDKIEQVPFDEGGETQEETETRVKKRAR :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05G IKEAEDITLVDKNGGNWPSYVASGDGQGGKYYLAKGWRSFCAANRLKTGETFTLEFVRGEGTTPMLKFCSKSKDKIEQVPFDEGGETQEETETRVKKRAR 310 320 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 480 AT4G31 VSAERGSSRRNQASNKPNADPQNLQPGQPHCSISDQVQKVKQIIVDTLTGIKRIRSELERKGQKLEASILEIDALGEKVSEINKILK :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05G VSAERGSSRRNQASNKPNADPQNLQPGQPHCSISDQVQKVKQIIVDTLTGIKRIRSELERKGQKLEASILEIDALGMI--------- 410 420 430 440 450 460 470

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