◆◆◆IE_05G09 detail◆◆◆

Code name	IE_05G09
Locus	AT1G12860
Template	IT_05G09 (HR3368)
Donor Vector	pDONR207
Forward primer	IPF_05G09 (ggggacaagtttgtacaaaaaagcaggcttcATGAACAGCGACGGTGTTTGGCTTGACG)
Reverse primer	IPR_05G09 (ggggaccactttgtacaagaaagctgggttAACCAAACCAGCGTAACCTGCTGTATCTAA)
Alignment with template seq.	1st	アミノ酸配列100%一致	2nd	-
Alignment with TAIR7CDS	1st	アミノ酸配列54.30%一致	2nd	-
comment	1st:Templateの配列とは一致
sequence	>IE_05G09_1350bp ATGAACAGCGACGGTGTTTGGCTTGACGGCTCCGGTGAATCTCCGGAAGTTAATAACGGTGAAGCTGCGTCTTGGGTCAGAAACCCAGATGAAGACTGGT TCAATAACCCACCACCACCACAACACACTAATCAAAACGACTTCAGATTCAATGGTGGCTTTCCTTTAAACCCCTCAGAGAATCTGCTTCTTCTTCTTCA GCAATCGATTGATTCTTCTTCTTCTTCTTCTCCGTTATTACATCCTTTCACACTCGACGCTGCTTCACAGCAACAACAACAACAACAACAACAACAGGAA CAGTCTTTCTTAGCTACGAAAGCTTGTATAGTTTCTCTTCTCAACGTCCCAACCATCAATAACAACACTTTCGATGACTTCGGCTTTGACTCTGGTTTCT TAGGACAACAATTCCATGGAAATCATCAATCTCCGAACTCGATGAATTTCACTGGCTTAAACCACTCAGTACCGGATTTTCTTCCAGCTCCGGAAAACAG CTCAGGATCATGTGGATTGAGTCCTCTGTTCTCAAACAGAGCAAAGGTTTTAAAACCGTTACAGGTAATGGCTTCATCTGGCTCGCAGCCAACTCTGTTT CAGAAACGAGCTGCAATGCGTCAGAGCTCGAGTAGCAAAATGTGCAATTCTGAGAGTTCTTCTGAAATGAGGAAATCGAGCTACGAGAGAGAGATTGACG ATACTAGTACCGGAATCATCGATATCTCTGGATTGAATTACGAATCTGATGACCATAATACTAATAACAACAAAGGTAAGAAGAAAGGAATGCCTGCAAA GAATCTTATGGCTGAGAGAAGAAGAAGGAAGAAGCTTAATGATAGGCTTTACATGCTTAGATCAGTTGTTCCCAAGATCAGCAAAATGGATAGAGCATCA ATACTTGGAGATGCTATTGATTACCTCAAAGAGCTTTTACAAAGAATCAACGATCTTCACACCGAACTTGAATCTACTCCACCGAGTTCTTCAAGCTTGC ATCCGTTAACACCGACTCCACAAACGCTGTCTTACCGTGTTAAGGAAGAGTTGTGTCCATCTTCCTCCTTGCCAAGTCCTAAAGGCCAACAACCAAGAGT TGAGGTTAGATTAAGAGAAGGAAAGGCAGTGAACATACACATGTTCTGTGGACGTAGACCAGGTCTTTTACTTTCCACCATGAGAGCTTTGGATAACTTA GGATTGGATGTTCAACAAGCGGTGATTAGCTGTTTCAATGGTTTTGCTTTGGATGTTTTCCGCGCTGAGCAATGTCAAGAAGACCATGACGTGTTACCTG AACAAATCAAAGCAGTGCTTTTAGATACAGCAGGTTACGCTGGTTTGGTT >IE_05G09_translated MNSDGVWLDGSGESPEVNNGEAASWVRNPDEDWFNNPPPPQHTNQNDFRFNGGFPLNPSENLLLLLQQSIDSSSSSSPLLHPFTLDAASQQQQQQQQQQE QSFLATKACIVSLLNVPTINNNTFDDFGFDSGFLGQQFHGNHQSPNSMNFTGLNHSVPDFLPAPENSSGSCGLSPLFSNRAKVLKPLQVMASSGSQPTLF QKRAAMRQSSSSKMCNSESSSEMRKSSYEREIDDTSTGIIDISGLNYESDDHNTNNNKGKKKGMPAKNLMAERRRRKKLNDRLYMLRSVVPKISKMDRAS ILGDAIDYLKELLQRINDLHTELESTPPSSSSLHPLTPTPQTLSYRVKEELCPSSSLPSPKGQQPRVEVRLREGKAVNIHMFCGRRPGLLLSTMRALDNL GLDVQQAVISCFNGFALDVFRAEQCQEDHDVLPEQIKAVLLDTAGYAGLV
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 1350/2484 (54.3%)（アミノ酸配列で450/828 (54.3%)）一致しました。 10 20 30 40 50 60 70 80 90 100 AT1G12 ATGGAGAGTAGAGAGGATTCATTCATATCAAAAGAGAAGAAATCAACTATGAAAAAAGAAAAACAAGCGATTGCATCACAAAGAAATAGAAGAAGAGTAA IE_05G ---------------------------------------------------------------------------------------------------- 110 120 130 140 150 160 170 180 190 200 AT1G12 TTAAAAATAGAGGAAACGGGAAGCGATTGATTGCATCATTATCACAACGAAAGAGAAGAAGAATACCTAGAGGAAGAGGAAACGAGAAGGCAGTGTTTGC IE_05G ---------------------------------------------------------------------------------------------------- 210 220 230 240 250 260 270 280 290 300 AT1G12 ACCATCATCACTACCAAACGATGTTGTAGAGGAAATCTTCTTACGACTTCCGGTGAAAGCAATAATCCAACTAAAGTCTCTCTCAAAACAATGGAGATCG IE_05G ---------------------------------------------------------------------------------------------------- 310 320 330 340 350 360 370 380 390 400 AT1G12 ACGATCGAGTCTCGCAGTTTTGAAGAGAGACACTTGAAGATCGTCGAGCGATCACGCGTGGATTTTCCACAGGTCATGGTCATGTCTGAAGAATATTCCT IE_05G ---------------------------------------------------------------------------------------------------- 410 420 430 440 450 460 470 480 490 500 AT1G12 TGAAAGGAAGTAAAGGTAATCAGCCTCGTCCAGATACAGACATTGGTTTTAGTACAATCTGTTTGGAATCGGCTTCTATTCTGTCCTCCACTCTTATCAC IE_05G ---------------------------------------------------------------------------------------------------- 510 520 530 540 550 560 570 580 590 600 AT1G12 TTTCCCTCAAGGATTTCAACACCGGATCTATGCTTCTGAAAGCTGTGACGGACTTTTCTGCATCCATTCCCTAAAAACACAAGCCATATATGTAGTTAAC IE_05G ---------------------------------------------------------------------------------------------------- 610 620 630 640 650 660 670 680 690 700 AT1G12 CCGGCCACTCGGTGGTTTCGACAACTTCCTCCGGCTAGGTTTCAGATTTTGATGCAAAAGTTATACCCCACTCAAGACACTTGGATTGACATAAAACCAG IE_05G ---------------------------------------------------------------------------------------------------- 710 720 730 740 750 760 770 780 790 800 AT1G12 TAGTCTGTTATACAGCATTCGTGAAGGCCAATGATTACAAATTAGTGTGGTTGTATAATTCTGATGCCTCGAATCCAAACCTGGGAGTTACAAAGTGCGA IE_05G ---------------------------------------------------------------------------------------------------- 810 820 830 840 850 860 870 880 890 900 AT1G12 GGTTTTTGATTTTAGGGCAAACGCTTGGAGGTACTTGACTTGCACTCCAAGCTATCGGATATTCCCTGACCAAGTACCAGCAGCTACAAACGGGTCTATT IE_05G ---------------------------------------------------------------------------------------------------- 910 920 930 940 950 960 970 980 990 1000 AT1G12 TATTGGTTCACGGAACCATATAATGGCGAAATAAAAGTGGTAGCTCTCGATATTCACACTGAAACGTTTCGTGTGCTACCTAAGATTAATCCGGCCATTG IE_05G ---------------------------------------------------------------------------------------------------- 1010 1020 1030 1040 1050 1060 1070 1080 1090 1100 AT1G12 CTAGTTCAGATCCTGACCATATCGACATGTGCACTTTGGATAATGGTTTGTGCATGTCGAAAAGAGAATCGGATACGTTGGTCCAAGAGATATGGAGGTT IE_05G ---------------------------------------------------------------------------------------------------- 1110 1120 1130 1140 1150 1160 1170 1180 1190 1200 AT1G12 AAAATCATCAGAAGACTCATGGGAGAAGTTTGACATGAACAGCGACGGTGTTTGGCTTGACGGCTCCGGTGAATCTCCGGAAGTTAATAACGGTGAAGCT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05G ----------------------------------ATGAACAGCGACGGTGTTTGGCTTGACGGCTCCGGTGAATCTCCGGAAGTTAATAACGGTGAAGCT 10 20 30 40 50 60 1210 1220 1230 1240 1250 1260 1270 1280 1290 1300 AT1G12 GCGTCTTGGGTCAGAAACCCAGATGAAGACTGGTTCAATAACCCACCACCACCACAACACACTAATCAAAACGACTTCAGATTCAATGGTGGCTTTCCTT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05G GCGTCTTGGGTCAGAAACCCAGATGAAGACTGGTTCAATAACCCACCACCACCACAACACACTAATCAAAACGACTTCAGATTCAATGGTGGCTTTCCTT 70 80 90 100 110 120 130 140 150 160 1310 1320 1330 1340 1350 1360 1370 1380 1390 1400 AT1G12 TAAACCCCTCAGAGAATCTGCTTCTTCTTCTTCAGCAATCGATTGATTCTTCTTCTTCTTCTTCTCCGTTATTACATCCTTTCACACTCGACGCTGCTTC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05G TAAACCCCTCAGAGAATCTGCTTCTTCTTCTTCAGCAATCGATTGATTCTTCTTCTTCTTCTTCTCCGTTATTACATCCTTTCACACTCGACGCTGCTTC 170 180 190 200 210 220 230 240 250 260 1410 1420 1430 1440 1450 1460 1470 1480 1490 1500 AT1G12 ACAGCAACAACAACAACAACAACAACAACAGGAACAGTCTTTCTTAGCTACGAAAGCTTGTATAGTTTCTCTTCTCAACGTCCCAACCATCAATAACAAC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05G ACAGCAACAACAACAACAACAACAACAACAGGAACAGTCTTTCTTAGCTACGAAAGCTTGTATAGTTTCTCTTCTCAACGTCCCAACCATCAATAACAAC 270 280 290 300 310 320 330 340 350 360 1510 1520 1530 1540 1550 1560 1570 1580 1590 1600 AT1G12 ACTTTCGATGACTTCGGCTTTGACTCTGGTTTCTTAGGACAACAATTCCATGGAAATCATCAATCTCCGAACTCGATGAATTTCACTGGCTTAAACCACT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05G ACTTTCGATGACTTCGGCTTTGACTCTGGTTTCTTAGGACAACAATTCCATGGAAATCATCAATCTCCGAACTCGATGAATTTCACTGGCTTAAACCACT 370 380 390 400 410 420 430 440 450 460 1610 1620 1630 1640 1650 1660 1670 1680 1690 1700 AT1G12 CAGTACCGGATTTTCTTCCAGCTCCGGAAAACAGCTCAGGATCATGTGGATTGAGTCCTCTGTTCTCAAACAGAGCAAAGGTTTTAAAACCGTTACAGGT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05G CAGTACCGGATTTTCTTCCAGCTCCGGAAAACAGCTCAGGATCATGTGGATTGAGTCCTCTGTTCTCAAACAGAGCAAAGGTTTTAAAACCGTTACAGGT 470 480 490 500 510 520 530 540 550 560 1710 1720 1730 1740 1750 1760 1770 1780 1790 1800 AT1G12 AATGGCTTCATCTGGCTCGCAGCCAACTCTGTTTCAGAAACGAGCTGCAATGCGTCAGAGCTCGAGTAGCAAAATGTGCAATTCTGAGAGTTCTTCTGAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05G AATGGCTTCATCTGGCTCGCAGCCAACTCTGTTTCAGAAACGAGCTGCAATGCGTCAGAGCTCGAGTAGCAAAATGTGCAATTCTGAGAGTTCTTCTGAA 570 580 590 600 610 620 630 640 650 660 1810 1820 1830 1840 1850 1860 1870 1880 1890 1900 AT1G12 ATGAGGAAATCGAGCTACGAGAGAGAGATTGACGATACTAGTACCGGAATCATCGATATCTCTGGATTGAATTACGAATCTGATGACCATAATACTAATA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05G ATGAGGAAATCGAGCTACGAGAGAGAGATTGACGATACTAGTACCGGAATCATCGATATCTCTGGATTGAATTACGAATCTGATGACCATAATACTAATA 670 680 690 700 710 720 730 740 750 760 1910 1920 1930 1940 1950 1960 1970 1980 1990 2000 AT1G12 ACAACAAAGGTAAGAAGAAAGGAATGCCTGCAAAGAATCTTATGGCTGAGAGAAGAAGAAGGAAGAAGCTTAATGATAGGCTTTACATGCTTAGATCAGT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05G ACAACAAAGGTAAGAAGAAAGGAATGCCTGCAAAGAATCTTATGGCTGAGAGAAGAAGAAGGAAGAAGCTTAATGATAGGCTTTACATGCTTAGATCAGT 770 780 790 800 810 820 830 840 850 860 2010 2020 2030 2040 2050 2060 2070 2080 2090 2100 AT1G12 TGTTCCCAAGATCAGCAAAATGGATAGAGCATCAATACTTGGAGATGCTATTGATTACCTCAAAGAGCTTTTACAAAGAATCAACGATCTTCACACCGAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05G TGTTCCCAAGATCAGCAAAATGGATAGAGCATCAATACTTGGAGATGCTATTGATTACCTCAAAGAGCTTTTACAAAGAATCAACGATCTTCACACCGAA 870 880 890 900 910 920 930 940 950 960 2110 2120 2130 2140 2150 2160 2170 2180 2190 2200 AT1G12 CTTGAATCTACTCCACCGAGTTCTTCAAGCTTGCATCCGTTAACACCGACTCCACAAACGCTGTCTTACCGTGTTAAGGAAGAGTTGTGTCCATCTTCCT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05G CTTGAATCTACTCCACCGAGTTCTTCAAGCTTGCATCCGTTAACACCGACTCCACAAACGCTGTCTTACCGTGTTAAGGAAGAGTTGTGTCCATCTTCCT 970 980 990 1000 1010 1020 1030 1040 1050 1060 2210 2220 2230 2240 2250 2260 2270 2280 2290 2300 AT1G12 CCTTGCCAAGTCCTAAAGGCCAACAACCAAGAGTTGAGGTTAGATTAAGAGAAGGAAAGGCAGTGAACATACACATGTTCTGTGGACGTAGACCAGGTCT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05G CCTTGCCAAGTCCTAAAGGCCAACAACCAAGAGTTGAGGTTAGATTAAGAGAAGGAAAGGCAGTGAACATACACATGTTCTGTGGACGTAGACCAGGTCT 1070 1080 1090 1100 1110 1120 1130 1140 1150 1160 2310 2320 2330 2340 2350 2360 2370 2380 2390 2400 AT1G12 TTTACTTTCCACCATGAGAGCTTTGGATAACTTAGGATTGGATGTTCAACAAGCGGTGATTAGCTGTTTCAATGGTTTTGCTTTGGATGTTTTCCGCGCT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05G TTTACTTTCCACCATGAGAGCTTTGGATAACTTAGGATTGGATGTTCAACAAGCGGTGATTAGCTGTTTCAATGGTTTTGCTTTGGATGTTTTCCGCGCT 1170 1180 1190 1200 1210 1220 1230 1240 1250 1260 2410 2420 2430 2440 2450 2460 2470 2480 AT1G12 GAGCAATGTCAAGAAGACCATGACGTGTTACCTGAACAAATCAAAGCAGTGCTTTTAGATACAGCAGGTTACGCTGGTTTGGTT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05G GAGCAATGTCAAGAAGACCATGACGTGTTACCTGAACAAATCAAAGCAGTGCTTTTAGATACAGCAGGTTACGCTGGTTTGGTT 1270 1280 1290 1300 1310 1320 1330 1340 1350 10 20 30 40 50 60 70 80 90 100 AT1G12 MESREDSFISKEKKSTMKKEKQAIASQRNRRRVIKNRGNGKRLIASLSQRKRRRIPRGRGNEKAVFAPSSLPNDVVEEIFLRLPVKAIIQLKSLSKQWRS IE_05G ---------------------------------------------------------------------------------------------------- 110 120 130 140 150 160 170 180 190 200 AT1G12 TIESRSFEERHLKIVERSRVDFPQVMVMSEEYSLKGSKGNQPRPDTDIGFSTICLESASILSSTLITFPQGFQHRIYASESCDGLFCIHSLKTQAIYVVN IE_05G ---------------------------------------------------------------------------------------------------- 210 220 230 240 250 260 270 280 290 300 AT1G12 PATRWFRQLPPARFQILMQKLYPTQDTWIDIKPVVCYTAFVKANDYKLVWLYNSDASNPNLGVTKCEVFDFRANAWRYLTCTPSYRIFPDQVPAATNGSI IE_05G ---------------------------------------------------------------------------------------------------- 310 320 330 340 350 360 370 380 390 400 AT1G12 YWFTEPYNGEIKVVALDIHTETFRVLPKINPAIASSDPDHIDMCTLDNGLCMSKRESDTLVQEIWRLKSSEDSWEKFDMNSDGVWLDGSGESPEVNNGEA :::::::::::::::::::::: IE_05G ------------------------------------------------------------------------------MNSDGVWLDGSGESPEVNNGEA 10 20 410 420 430 440 450 460 470 480 490 500 AT1G12 ASWVRNPDEDWFNNPPPPQHTNQNDFRFNGGFPLNPSENLLLLLQQSIDSSSSSSPLLHPFTLDAASQQQQQQQQQQEQSFLATKACIVSLLNVPTINNN :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05G ASWVRNPDEDWFNNPPPPQHTNQNDFRFNGGFPLNPSENLLLLLQQSIDSSSSSSPLLHPFTLDAASQQQQQQQQQQEQSFLATKACIVSLLNVPTINNN 30 40 50 60 70 80 90 100 110 120 510 520 530 540 550 560 570 580 590 600 AT1G12 TFDDFGFDSGFLGQQFHGNHQSPNSMNFTGLNHSVPDFLPAPENSSGSCGLSPLFSNRAKVLKPLQVMASSGSQPTLFQKRAAMRQSSSSKMCNSESSSE :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05G TFDDFGFDSGFLGQQFHGNHQSPNSMNFTGLNHSVPDFLPAPENSSGSCGLSPLFSNRAKVLKPLQVMASSGSQPTLFQKRAAMRQSSSSKMCNSESSSE 130 140 150 160 170 180 190 200 210 220 610 620 630 640 650 660 670 680 690 700 AT1G12 MRKSSYEREIDDTSTGIIDISGLNYESDDHNTNNNKGKKKGMPAKNLMAERRRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQRINDLHTE :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05G MRKSSYEREIDDTSTGIIDISGLNYESDDHNTNNNKGKKKGMPAKNLMAERRRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQRINDLHTE 230 240 250 260 270 280 290 300 310 320 710 720 730 740 750 760 770 780 790 800 AT1G12 LESTPPSSSSLHPLTPTPQTLSYRVKEELCPSSSLPSPKGQQPRVEVRLREGKAVNIHMFCGRRPGLLLSTMRALDNLGLDVQQAVISCFNGFALDVFRA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05G LESTPPSSSSLHPLTPTPQTLSYRVKEELCPSSSLPSPKGQQPRVEVRLREGKAVNIHMFCGRRPGLLLSTMRALDNLGLDVQQAVISCFNGFALDVFRA 330 340 350 360 370 380 390 400 410 420 810 820 AT1G12 EQCQEDHDVLPEQIKAVLLDTAGYAGLV :::::::::::::::::::::::::::: IE_05G EQCQEDHDVLPEQIKAVLLDTAGYAGLV 430 440 450

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