◆◆◆IE_05G11 detail◆◆◆

Code name	IE_05G11
Locus	AT3G26744
Template	IT_05G11 (HR3394)
Donor Vector	pDONR207
Forward primer	IPF_05G11 (ggggacaagtttgtacaaaaaagcaggcttcATGGATAGAGCATCAATACTTGGAGATG)
Reverse primer	IPR_05G11 (ggggaccactttgtacaagaaagctgggttGATCATACCAGCATACCCTGCTGTATCGAA)
Alignment with template seq.	1st	アミノ酸配列100%一致	2nd	-
Alignment with TAIR7CDS	1st	アミノ酸配列32.20%一致	2nd	-
comment	1st:Templateの配列とは一致
sequence	>IE_05G11_477bp ATGGATAGAGCATCAATACTTGGAGATGCAATTGATTATCTGAAGGAACTTCTACAAAGGATCAATGATCTTCACAATGAACTTGAGTCAACTCCTCCTG GATCTCTGCCTCCAACTTCATCAAGCTTCCATCCGTTGACACCTACACCGCAAACTCTTTCTTGTCGTGTCAAGGAAGAGTTGTGTCCCTCTTCTTTACC AAGTCCTAAAGGCCAGCAAGCTAGAGTTGAGGTTAGATTAAGGGAAGGAAGAGCAGTGAACATTCATATGTTCTGTGGTCGTAGACCGGGTCTGTTGCTC GCTACCATGAAAGCTTTGGATAATCTTGGATTGGATGTTCAGCAAGCTGTGATCAGCTGTTTTAATGGGTTTGCCTTGGATGTTTTCCGCGCTGAGCAAT GCCAAGAAGGACAAGAGATACTGCCTGATCAAATCAAAGCAGTGCTTTTCGATACAGCAGGGTATGCTGGTATGATC >IE_05G11_translated MDRASILGDAIDYLKELLQRINDLHNELESTPPGSLPPTSSSFHPLTPTPQTLSCRVKEELCPSSLPSPKGQQARVEVRLREGRAVNIHMFCGRRPGLLL ATMKALDNLGLDVQQAVISCFNGFALDVFRAEQCQEGQEILPDQIKAVLFDTAGYAGMI
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 476/1482 (32.1%)（アミノ酸配列で159/494 (32.2%)）一致しました。 10 20 30 40 50 60 70 80 90 100 AT3G26 ATGGGTCTTGACGGAAACAATGGTGGAGGGGTTTGGTTAAACGGTGGTGGTGGAGAAAGGGAAGAGAACGAGGAAGGTTCATGGGGAAGGAATCAAGAAG IE_05G ---------------------------------------------------------------------------------------------------- 110 120 130 140 150 160 170 180 190 200 AT3G26 ATGGTTCTTCTCAGTTTAAGCCTATGCTTGAAGGTGATTGGTTTAGTAGTAACCAACCACATCCACAAGATCTTCAGATGTTACAGAATCAGCCAGATTT IE_05G ---------------------------------------------------------------------------------------------------- 210 220 230 240 250 260 270 280 290 300 AT3G26 CAGATACTTTGGTGGTTTTCCTTTTAACCCTAATGATAATCTTCTTCTTCAACACTCTATTGATTCTTCTTCTTCTTGTTCTCCTTCTCAAGCTTTTAGT IE_05G ---------------------------------------------------------------------------------------------------- 310 320 330 340 350 360 370 380 390 400 AT3G26 CTTGACCCTTCTCAGCAAAATCAGTTCTTGTCAACTAACAACAACAAGGGTTGTCTTCTCAATGTTCCTTCTTCTGCAAACCCTTTTGATAATGCTTTTG IE_05G ---------------------------------------------------------------------------------------------------- 410 420 430 440 450 460 470 480 490 500 AT3G26 AGTTTGGCTCTGAATCTGGTTTTCTTAACCAAATCCATGCTCCTATTTCGATGGGGTTTGGTTCTTTGACACAATTGGGGAACAGGGATTTGAGTTCTGT IE_05G ---------------------------------------------------------------------------------------------------- 510 520 530 540 550 560 570 580 590 600 AT3G26 TCCTGATTTCTTGTCTGCTCGGTCACTTCTTGCGCCGGAAAGCAACAACAACAACACAATGTTGTGTGGTGGTTTCACAGCTCCGTTGGAGTTGGAAGGT IE_05G ---------------------------------------------------------------------------------------------------- 610 620 630 640 650 660 670 680 690 700 AT3G26 TTTGGTAGTCCTGCTAATGGTGGTTTTGTTGGGAACAGAGCGAAAGTTCTGAAGCCTTTAGAGGTGTTAGCATCGTCTGGTGCACAGCCTACTCTGTTCC IE_05G ---------------------------------------------------------------------------------------------------- 710 720 730 740 750 760 770 780 790 800 AT3G26 AGAAACGTGCAGCTATGCGTCAGAGCTCTGGAAGCAAAATGGGAAATTCGGAGAGTTCGGGAATGAGGAGGTTTAGTGATGATGGAGATATGGATGAGAC IE_05G ---------------------------------------------------------------------------------------------------- 810 820 830 840 850 860 870 880 890 900 AT3G26 TGGGATTGAGGTTTCTGGGTTGAACTATGAGTCTGATGAGATAAATGAGAGCGGTAAAGCGGCTGAGAGTGTTCAGATTGGAGGAGGAGGAAAGGGTAAG IE_05G ---------------------------------------------------------------------------------------------------- 910 920 930 940 950 960 970 980 990 1000 AT3G26 AAGAAAGGTATGCCTGCTAAGAATCTGATGGCTGAGAGGAGAAGGAGGAAGAAGCTTAATGATAGGCTTTATATGCTTAGATCAGTTGTCCCCAAGATCA IE_05G ---------------------------------------------------------------------------------------------------- 1010 1020 1030 1040 1050 1060 1070 1080 1090 1100 AT3G26 GCAAAATGGATAGAGCATCAATACTTGGAGATGCAATTGATTATCTGAAGGAACTTCTACAAAGGATCAATGATCTTCACAATGAACTTGAGTCAACTCC ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05G -----ATGGATAGAGCATCAATACTTGGAGATGCAATTGATTATCTGAAGGAACTTCTACAAAGGATCAATGATCTTCACAATGAACTTGAGTCAACTCC 10 20 30 40 50 60 70 80 90 1110 1120 1130 1140 1150 1160 1170 1180 1190 1200 AT3G26 TCCTGGATCTTTGCCTCCAACTTCATCAAGCTTCCATCCGTTGACACCTACACCGCAAACTCTTTCTTGTCGTGTCAAGGAAGAGTTGTGTCCCTCTTCT :::::::::: ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05G TCCTGGATCTCTGCCTCCAACTTCATCAAGCTTCCATCCGTTGACACCTACACCGCAAACTCTTTCTTGTCGTGTCAAGGAAGAGTTGTGTCCCTCTTCT 100 110 120 130 140 150 160 170 180 190 1210 1220 1230 1240 1250 1260 1270 1280 1290 1300 AT3G26 TTACCAAGTCCTAAAGGCCAGCAAGCTAGAGTTGAGGTTAGATTAAGGGAAGGAAGAGCAGTGAACATTCATATGTTCTGTGGTCGTAGACCGGGTCTGT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05G TTACCAAGTCCTAAAGGCCAGCAAGCTAGAGTTGAGGTTAGATTAAGGGAAGGAAGAGCAGTGAACATTCATATGTTCTGTGGTCGTAGACCGGGTCTGT 200 210 220 230 240 250 260 270 280 290 1310 1320 1330 1340 1350 1360 1370 1380 1390 1400 AT3G26 TGCTCGCTACCATGAAAGCTTTGGATAATCTTGGATTGGATGTTCAGCAAGCTGTGATCAGCTGTTTTAATGGGTTTGCCTTGGATGTTTTCCGCGCTGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05G TGCTCGCTACCATGAAAGCTTTGGATAATCTTGGATTGGATGTTCAGCAAGCTGTGATCAGCTGTTTTAATGGGTTTGCCTTGGATGTTTTCCGCGCTGA 300 310 320 330 340 350 360 370 380 390 1410 1420 1430 1440 1450 1460 1470 1480 AT3G26 GCAATGCCAAGAAGGACAAGAGATACTGCCTGATCAAATCAAAGCAGTGCTTTTCGATACAGCAGGGTATGCTGGTATGATC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05G GCAATGCCAAGAAGGACAAGAGATACTGCCTGATCAAATCAAAGCAGTGCTTTTCGATACAGCAGGGTATGCTGGTATGATC 400 410 420 430 440 450 460 470 10 20 30 40 50 60 70 80 90 100 AT3G26 MGLDGNNGGGVWLNGGGGEREENEEGSWGRNQEDGSSQFKPMLEGDWFSSNQPHPQDLQMLQNQPDFRYFGGFPFNPNDNLLLQHSIDSSSSCSPSQAFS IE_05G ---------------------------------------------------------------------------------------------------- 110 120 130 140 150 160 170 180 190 200 AT3G26 LDPSQQNQFLSTNNNKGCLLNVPSSANPFDNAFEFGSESGFLNQIHAPISMGFGSLTQLGNRDLSSVPDFLSARSLLAPESNNNNTMLCGGFTAPLELEG IE_05G ---------------------------------------------------------------------------------------------------- 210 220 230 240 250 260 270 280 290 300 AT3G26 FGSPANGGFVGNRAKVLKPLEVLASSGAQPTLFQKRAAMRQSSGSKMGNSESSGMRRFSDDGDMDETGIEVSGLNYESDEINESGKAAESVQIGGGGKGK IE_05G ---------------------------------------------------------------------------------------------------- 310 320 330 340 350 360 370 380 390 400 AT3G26 KKGMPAKNLMAERRRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQRINDLHNELESTPPGSLPPTSSSFHPLTPTPQTLSCRVKEELCPSS ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05G -----------------------------------MDRASILGDAIDYLKELLQRINDLHNELESTPPGSLPPTSSSFHPLTPTPQTLSCRVKEELCPSS 10 20 30 40 50 60 410 420 430 440 450 460 470 480 490 AT3G26 LPSPKGQQARVEVRLREGRAVNIHMFCGRRPGLLLATMKALDNLGLDVQQAVISCFNGFALDVFRAEQCQEGQEILPDQIKAVLFDTAGYAGMI :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05G LPSPKGQQARVEVRLREGRAVNIHMFCGRRPGLLLATMKALDNLGLDVQQAVISCFNGFALDVFRAEQCQEGQEILPDQIKAVLFDTAGYAGMI 70 80 90 100 110 120 130 140 150

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