| Code name | IE_05H04 | |||
|---|---|---|---|---|
| Locus | AT3G04930 | |||
| Template | IT_05H04 (HR3527) | |||
| Donor Vector | pDONR207 | |||
| Forward primer | IPF_05H04 (ggggacaagtttgtacaaaaaagcaggcttcATGACATCGGATCACCGCGACGCTCTTT) | |||
| Reverse primer | IPR_05H04 (ggggaccactttgtacaagaaagctgggttTATACCACTCGGCATCGTCTTTAACTCGTT) | |||
| Alignment with template seq. | 1st | 配列解析が行えなかったサンプルです。 | 2nd | アミノ酸配列100%一致 |
| Alignment with TAIR7CDS | - | アミノ酸配列100%一致 | ||
| comment | 1st:(Sequence解析まで行ったが、読み直す必要がある) | |||
| sequence |
>IE_05H04_1368bp ATGACATCGGATCACCGCGACGCTCTTTTCTCTCTGGAACTGGAATCGCCTGATCCGGACGAAGGAGGTGTAGCAGACGGAGGAGAATCTGATACCGACG AGGATTTGCGCGACAACGATGATGTTGTTATGCCGGGAACGAACGAAGCTGAAGCTGAAGACGACGATCCCGAAGAGGAAGATCTCAATTCTCCGTCTAC ATCATCACTCCCCATGGTTTCTACGATCTCAGCGACTGCTGTTGTTTCCGGGACCCCGACGGCGACTTCCTCTACAGGCGCCGTGACCGTTGCACTTCCG GCTGGATCCGCCGTCCCTGTCTCCGTGATTCCCGTTGATTCCGATCCGAAGTGGCACCGTATGACGGAGATCGTCCATCAGAGACCACCGATCGATGATT CTAGACGCCTTTTCCAGAGGCTGTGGACAGACGAAGACGAGATCGAGCTTCTTCGTGGTTTTCTCGATTACATGACGATGCACCGCGGAAGCAGCTCGCA TCCGCCGGATACGGCGCCGTTTTATGAGCAGATAAAGTCGAAACTTCAGCTGGATTTCAACAAGAACCAGCTGGTGGAGAAGCTTCGGAGATTGAAGAAG AAGTACAGGAATGTGATGAGCAAGATCAGCTCTGGTAAAGAGGTATTCTTCAAGAGCCCTCATGATCAGTCTACTTTCGAGATTTCTAGAAAAATCTGGA ACCAAACTGGGAAAATCATAGGCTTTGAAGATAACAACGTAATGGATTTCGAAGAAACCAATAACCATCATAACACCACCAATGGTAACTACTCAACCTT TAACAGCCCTAGTTCTAATCCTACTCTGGAGCTCGACTCCGAAAATGGAATCGAGAAGAAATTGACAATGAGTAGCAGTAGCGTGTCGAGAAAACGATCA CGATCAAGAATTGGGAAGATTGAGGAAGACAACAAACCTGTCATCACTCCCTCTGATGGGCCAATAGCTAGCAATGTCAACCTCAACGAAGCTGCAGCTG TTGGTATTGGAGGAAATCTCGGGGGTTTGATCGAAGAGACTGTGAAAAACTGTGTCTCTCCTGTGATCAAAGAGATGATGAATGGGACAACAAGTATGAT GATGGCTGCAATGGGAGGAGGGTTTCCGGGTCTTGGTGGTGGTGGTGGTGGTGGTGGTGGTCATGGTTTTGGCTCGCTAAGCCCCATGTTTACACGGCCG TTCAATTTCGGTTTTGGTGTAGAGGGAGGAGGAAACAAGGCGGTTGCAGATGAGCGGTGGAGGAAACAACAGATTCTGGAATTGGAAGTGTATTCAAGGA GATTAGAGTTGGTTCAAGAGCAGATTAGAACGACATTGAACGAGTTAAAGACGATGCCGAGTGGTATA >IE_05H04_translated MTSDHRDALFSLELESPDPDEGGVADGGESDTDEDLRDNDDVVMPGTNEAEAEDDDPEEEDLNSPSTSSLPMVSTISATAVVSGTPTATSSTGAVTVALP AGSAVPVSVIPVDSDPKWHRMTEIVHQRPPIDDSRRLFQRLWTDEDEIELLRGFLDYMTMHRGSSSHPPDTAPFYEQIKSKLQLDFNKNQLVEKLRRLKK KYRNVMSKISSGKEVFFKSPHDQSTFEISRKIWNQTGKIIGFEDNNVMDFEETNNHHNTTNGNYSTFNSPSSNPTLELDSENGIEKKLTMSSSSVSRKRS RSRIGKIEEDNKPVITPSDGPIASNVNLNEAAAVGIGGNLGGLIEETVKNCVSPVIKEMMNGTTSMMMAAMGGGFPGLGGGGGGGGGHGFGSLSPMFTRP FNFGFGVEGGGNKAVADERWRKQQILELEVYSRRLELVQEQIRTTLNELKTMPSGI |
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| Alignment |
◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 100%一致しました。 |
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