◆◆◆IE_05H06（IE_09C01） detail◆◆◆

Code nameIE_05H06（IE_09C01）
LocusAT1G04880
TemplateIT_05H06 (HR3708)
Donor VectorpDONR207
Forward primerIPF_05H06 (ggggacaagtttgtacaaaaaagcaggcttcATGGCATCAAGCTCTTGTCTCAAGCAAG)
Reverse primerIPR_05H06 (ggggaccactttgtacaagaaagctgggttGTTCTGCTCAGCAGTCACCGCCACAGTTGG)
Alignment with template seq.1stアミノ酸配列98.90%一致2ndアミノ酸配列100%一致
Alignment with TAIR7CDSアミノ酸配列98.90%一致アミノ酸配列100%一致
comment1st:(Sequence解析まで行ったが、フレームがずれる)→2nd(09C01):100%
sequence 
>IE_05H06_1344bp
ATGGCATCAAGCTCTTGTCTCAAGCAAGGGTCAGTGCCAATGAACAATGTTTGTGTTACCCCTGAAGCAACGTATGAGGCTGTGGTTGCAGACCCTAGGC
TTTTCATGACTTCCTTGGAAAGGCTGCACTCTCTCTTGGGTACTAAATTCATGGTTCCGATCATAGGAGGAAGAGATTTGGACTTGCACAAGCTTTTCGT
TGAGGTAACCTCACGTGGCGGAATCAATAAGATACTTAATGAAAGGAGATGGAAAGAAGTAACTGCTACATTTGTCTTTCCTCCAACTGCAACAAACGCA
TCATATGTTCTGCGCAAATACTACTTCTCGCTGCTGAACAATTATGAGCAAATCTATTTCTTCAGATCTAATGGTCAGATTCCTCCAGACTCTATGCAGA
GTCCATCTGCTAGACCATGCTTTATTCAGGGAGCCATTAGACCTTCACAAGAACTTCAAGCTCTAACCTTTACCCCACAGCCCAAGATCAATACTGCAGA
ATTCCTCGGAGGATCACTTGCTGGATCAAATGTGGTTGGAGTGATCGATGGAAAATTCGAAAGTGGTTACCTTGTAACTGTAACAATTGGATCAGAGCAG
CTTAAAGGAGTTCTGTATCAGCTTCTTCCTCAGAACACAGTTTCTTATCAAACTCCGCAACAGAGTCATGGTGTCCTCCCGAACACTCTGAACATTTCTG
CCAATCCTCAGGGGGTTGCAGGAGGAGTAACAAAACGCCGTAGGAGAAGAAAGAAATCAGAGATCAAAAGACGTGATCCTGATCATCCAAAACCCAACAG
AAGTGGTTATAACTTCTTTTTCGCAGAGCAGCACGCGAGACTTAAACCGCTTCATCCTGGTAAGGATAGGGATATCAGCAGGATGATTGGTGAGCTATGG
AACAAGCTGAATGAAGACGAAAAATTGATTTACCAAGGGAAGGCAATGGAGGACAAAGAGCGATACAGAACTGAAATGGAAGATTACAGAGAGAAAAAAA
AGAATGGACAGCTCATAAGCAACGCTGTTCCGCTGCAACAGAGACTTCCGGAGCAAAACGTAGACATGGCTGAAGCTGACCTTCCAATTGATGAAGTCGA
AGAAGATGACGAAGAAGGTGATTCAAGCGGATCCTCAGGCGAAAGCGAGCCACATGATGATCAGAGCATAGAAACTGATCCTGAATTGGAAGAGCCATCT
CTGAATCCCTCAGGTCCAAACCTAAACCCTAATCCGACTGAGATAGTGGTGGCTCCTAAGGAGAAAAACGGCGATGTTGTGATGGAAACATCTCCATTGA
AGAAAGCCGATGAGCCAACTGTGGCGGTGACTGCTGAGCAGAAC

>IE_05H06_translated
MASSSCLKQGSVPMNNVCVTPEATYEAVVADPRLFMTSLERLHSLLGTKFMVPIIGGRDLDLHKLFVEVTSRGGINKILNERRWKEVTATFVFPPTATNA
SYVLRKYYFSLLNNYEQIYFFRSNGQIPPDSMQSPSARPCFIQGAIRPSQELQALTFTPQPKINTAEFLGGSLAGSNVVGVIDGKFESGYLVTVTIGSEQ
LKGVLYQLLPQNTVSYQTPQQSHGVLPNTLNISANPQGVAGGVTKRRRRRKKSEIKRRDPDHPKPNRSGYNFFFAEQHARLKPLHPGKDRDISRMIGELW
NKLNEDEKLIYQGKAMEDKERYRTEMEDYREKKKNGQLISNAVPLQQRLPEQNVDMAEADLPIDEVEEDDEEGDSSGSSGESEPHDDQSIETDPELEEPS
LNPSGPNLNPNPTEIVVAPKEKNGDVVMETSPLKKADEPTVAVTAEQN
Alignment ◆CDS（TAIRver.9）_without_stop_codonとのalignment◆

100%一致しました。

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