◆◆◆IE_05H07 detail◆◆◆

Code name	IE_05H07
Locus	AT1G64625
Template	IT_05H07 (HR3750)
Donor Vector	pDONR207
Forward primer	IPF_05H07 (ggggacaagtttgtacaaaaaagcaggcttcATGGGTTCAGAGTATAAGCATATATTGA)
Reverse primer	IPR_05H07 (ggggaccactttgtacaagaaagctgggttTGATAATAAATCATCATGTTTGGTGTGGTG)
Alignment with template seq.	1st	アミノ酸配列100%一致	2nd	-
Alignment with TAIR7CDS	1st	アミノ酸配列98.10%一致	2nd	-
comment	1st:Templateの配列とは一致
sequence	>IE_05H07_1551bp ATGGGTTCAGAGTATAAGCATATATTGAAGAGTCTCTGTTTAAGCCATGGATGGTCTTATGCTGTGTTTTGGCGTTATGATCCCATCAATTCCATGATAT TGAGATTTGAAGAAGCATACAATGATGAACAATCAGTCGCACTAGTTGATGATATGGTTCTCCAGGCTCCTATCTTAGGCCAAGGAATTGTGGGAGAAGT AGCTTCATCCGGAAACCATCAATGGCTGTTCTCAGACACATTGTTTCAGTGGGAACATGAGTTTCAGAATCAGTTTCTCTGTGGCTTTAAGACTATCGCA ATCATTCCACTAGGATCCAGCGGCGTTGTTCAGCTAGGATCTACTCAAAAGATTCTTGAGAGCACAGAGATATTGGAACAAACAACAAGAGCTCTCCAGG AGACGTGTTTAAAGCCACATGATTCCGGTGATCTCGATACTCTGTTTGAGTCACTGGGAGATTGTGAGATTTTCCCAGCTGAATCTTTTCAAGGATTTAG CTTTGATGACATCTTCGCTGAGGACAATCCTCCTTCTTTGCTCAGCCCTGAGATGATCTCCTCTGAAGCAGCTTCTTCCAACCAAGATCTGACCAATGGA GATGATTATGGGTTCGACATTCTCCAGTCTTACTCTCTGGACGATCTATACCAGCTGCTCGCTGATCCACCAGAACAGAACTGCTCTTCCATGGTGATCC AAGGAGTTGACAAGGATCTTTTCGACATTTTGGGGATGAATTCTCAAACGCCTACAATGGCTTTGCCACCTAAAGGACTCTTCTCAGAGCTCATAAGCAG CAGTCTTAGCAACAACACTTGTAGTAGTTCTCTCACGAACGTGCAAGAGTACTCTGGTGTGAACCAGAGCAAAAGACGGAAGCTTGATACATCATCGGCG CATAGCTCAAGCTTGTTCCCTCAGGAGGAGACAGTAACAAGTCGGAGTTTGTGGATTGATGATGATGAGAGATCAAGTATTGGAGGGAACTGGAAGAAGC CTCACGAAGAAGGTGTGAAGAAGAAAAGAGCAAAGGCAGGAGAAAGCAGGAGGCCGAGGCCTAAGGACCGTCAGATGATACAAGACCGTATCAAGGAGTT GCGAGGGATGATTCCAAATGGAGCTAAGTGTAGCATAGATACGTTGCTGGATCTGACCATAAAACACATGGTGTTCATGCAAAGCCTCGCCAAGTATGCA GAGAGACTCAAACAACCATACGAGTCTAAGCTGGTGAAGGAGAAAGAGAGAACATGGGCGTTGGAAGTGGGGGAAGAAGGAGTGGTATGTCCGATAATGG TGGAGGAATTGAACAGGGAAGGAGAAATGCAGATAGAGATGGTGTGTGAGGAAAGAGAGGAGTTCTTGGAGATAGGACAAGTGGTGAGAGGATTGGGATT GAAGATATTGAAAGGAGTGATGGAAACAAGAAAAGGACAAATATGGGCACACTTCATCGTACAGGCAAAGCCACAAGTCACAAGGATTCAAGTCTTGTAC TCACTTGTTCAGCTCTTTCAACACCACACCAAACATGATGATTTATTATCA >IE_05H07_translated MGSEYKHILKSLCLSHGWSYAVFWRYDPINSMILRFEEAYNDEQSVALVDDMVLQAPILGQGIVGEVASSGNHQWLFSDTLFQWEHEFQNQFLCGFKTIA IIPLGSSGVVQLGSTQKILESTEILEQTTRALQETCLKPHDSGDLDTLFESLGDCEIFPAESFQGFSFDDIFAEDNPPSLLSPEMISSEAASSNQDLTNG DDYGFDILQSYSLDDLYQLLADPPEQNCSSMVIQGVDKDLFDILGMNSQTPTMALPPKGLFSELISSSLSNNTCSSSLTNVQEYSGVNQSKRRKLDTSSA HSSSLFPQEETVTSRSLWIDDDERSSIGGNWKKPHEEGVKKKRAKAGESRRPRPKDRQMIQDRIKELRGMIPNGAKCSIDTLLDLTIKHMVFMQSLAKYA ERLKQPYESKLVKEKERTWALEVGEEGVVCPIMVEELNREGEMQIEMVCEEREEFLEIGQVVRGLGLKILKGVMETRKGQIWAHFIVQAKPQVTRIQVLY SLVQLFQHHTKHDDLLS
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 1551/1581 (98.1%)（アミノ酸配列で517/527 (98.1%)）一致しました。 10 20 30 40 50 60 70 80 90 100 AT1G64 ATGGGTTCAGAGTATAAGCATATATTGAAGAGTCTCTGTTTAAGCCATGGATGGTCTTATGCTGTGTTTTGGCGTTATGATCCCATCAATTCCATGATAT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05H ATGGGTTCAGAGTATAAGCATATATTGAAGAGTCTCTGTTTAAGCCATGGATGGTCTTATGCTGTGTTTTGGCGTTATGATCCCATCAATTCCATGATAT 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT1G64 TGAGATTTGAAGAAGCATACAATGATGAACAATCAGTCGCACTAGTTGATGATATGGTTCTCCAGGCTCCTATCTTAGGCCAAGGAATTGTGGGAGAAGT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05H TGAGATTTGAAGAAGCATACAATGATGAACAATCAGTCGCACTAGTTGATGATATGGTTCTCCAGGCTCCTATCTTAGGCCAAGGAATTGTGGGAGAAGT 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT1G64 AGCTTCATCCGGAAACCATCAATGGCTGTTCTCAGACACATTGTTTCAGTGGGAACATGAGTTTCAGAATCAGTTTCTCTGTGGCTTTAAGATTCTTATA ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05H AGCTTCATCCGGAAACCATCAATGGCTGTTCTCAGACACATTGTTTCAGTGGGAACATGAGTTTCAGAATCAGTTTCTCTGTGGCTTTA----------- 210 220 230 240 250 260 270 280 310 320 330 340 350 360 370 380 390 400 AT1G64 CGGCAGTTTACTTATACACAGACTATCGCAATCATTCCACTAGGATCCAGCGGCGTTGTTCAGCTAGGATCTACTCAAAAGATTCTTGAGAGCACAGAGA ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05H -------------------AGACTATCGCAATCATTCCACTAGGATCCAGCGGCGTTGTTCAGCTAGGATCTACTCAAAAGATTCTTGAGAGCACAGAGA 290 300 310 320 330 340 350 360 370 410 420 430 440 450 460 470 480 490 500 AT1G64 TATTGGAACAAACAACAAGAGCTCTCCAGGAGACGTGTTTAAAGCCACATGATTCCGGTGATCTCGATACTCTGTTTGAGTCACTGGGAGATTGTGAGAT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05H TATTGGAACAAACAACAAGAGCTCTCCAGGAGACGTGTTTAAAGCCACATGATTCCGGTGATCTCGATACTCTGTTTGAGTCACTGGGAGATTGTGAGAT 380 390 400 410 420 430 440 450 460 470 510 520 530 540 550 560 570 580 590 600 AT1G64 TTTCCCAGCTGAATCTTTTCAAGGATTTAGCTTTGATGACATCTTCGCTGAGGACAATCCTCCTTCTTTGCTCAGCCCTGAGATGATCTCCTCTGAAGCA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05H TTTCCCAGCTGAATCTTTTCAAGGATTTAGCTTTGATGACATCTTCGCTGAGGACAATCCTCCTTCTTTGCTCAGCCCTGAGATGATCTCCTCTGAAGCA 480 490 500 510 520 530 540 550 560 570 610 620 630 640 650 660 670 680 690 700 AT1G64 GCTTCTTCCAACCAAGATCTGACCAATGGAGATGATTATGGGTTCGACATTCTCCAGTCTTACTCTCTGGACGATCTATACCAGCTGCTCGCTGATCCAC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05H GCTTCTTCCAACCAAGATCTGACCAATGGAGATGATTATGGGTTCGACATTCTCCAGTCTTACTCTCTGGACGATCTATACCAGCTGCTCGCTGATCCAC 580 590 600 610 620 630 640 650 660 670 710 720 730 740 750 760 770 780 790 800 AT1G64 CAGAACAGAACTGCTCTTCCATGGTGATCCAAGGAGTTGACAAGGATCTTTTCGACATTTTGGGGATGAATTCTCAAACGCCTACAATGGCTTTGCCACC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05H CAGAACAGAACTGCTCTTCCATGGTGATCCAAGGAGTTGACAAGGATCTTTTCGACATTTTGGGGATGAATTCTCAAACGCCTACAATGGCTTTGCCACC 680 690 700 710 720 730 740 750 760 770 810 820 830 840 850 860 870 880 890 900 AT1G64 TAAAGGACTCTTCTCAGAGCTCATAAGCAGCAGTCTTAGCAACAACACTTGTAGTAGTTCTCTCACGAACGTGCAAGAGTACTCTGGTGTGAACCAGAGC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05H TAAAGGACTCTTCTCAGAGCTCATAAGCAGCAGTCTTAGCAACAACACTTGTAGTAGTTCTCTCACGAACGTGCAAGAGTACTCTGGTGTGAACCAGAGC 780 790 800 810 820 830 840 850 860 870 910 920 930 940 950 960 970 980 990 1000 AT1G64 AAAAGACGGAAGCTTGATACATCATCGGCGCATAGCTCAAGCTTGTTCCCTCAGGAGGAGACAGTAACAAGTCGGAGTTTGTGGATTGATGATGATGAGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05H AAAAGACGGAAGCTTGATACATCATCGGCGCATAGCTCAAGCTTGTTCCCTCAGGAGGAGACAGTAACAAGTCGGAGTTTGTGGATTGATGATGATGAGA 880 890 900 910 920 930 940 950 960 970 1010 1020 1030 1040 1050 1060 1070 1080 1090 1100 AT1G64 GATCAAGTATTGGAGGGAACTGGAAGAAGCCTCACGAAGAAGGTGTGAAGAAGAAAAGAGCAAAGGCAGGAGAAAGCAGGAGGCCGAGGCCTAAGGACCG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05H GATCAAGTATTGGAGGGAACTGGAAGAAGCCTCACGAAGAAGGTGTGAAGAAGAAAAGAGCAAAGGCAGGAGAAAGCAGGAGGCCGAGGCCTAAGGACCG 980 990 1000 1010 1020 1030 1040 1050 1060 1070 1110 1120 1130 1140 1150 1160 1170 1180 1190 1200 AT1G64 TCAGATGATACAAGACCGTATCAAGGAGTTGCGAGGGATGATTCCAAATGGAGCTAAGTGTAGCATAGATACGTTGCTGGATCTGACCATAAAACACATG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05H TCAGATGATACAAGACCGTATCAAGGAGTTGCGAGGGATGATTCCAAATGGAGCTAAGTGTAGCATAGATACGTTGCTGGATCTGACCATAAAACACATG 1080 1090 1100 1110 1120 1130 1140 1150 1160 1170 1210 1220 1230 1240 1250 1260 1270 1280 1290 1300 AT1G64 GTGTTCATGCAAAGCCTCGCCAAGTATGCAGAGAGACTCAAACAACCATACGAGTCTAAGCTGGTGAAGGAGAAAGAGAGAACATGGGCGTTGGAAGTGG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05H GTGTTCATGCAAAGCCTCGCCAAGTATGCAGAGAGACTCAAACAACCATACGAGTCTAAGCTGGTGAAGGAGAAAGAGAGAACATGGGCGTTGGAAGTGG 1180 1190 1200 1210 1220 1230 1240 1250 1260 1270 1310 1320 1330 1340 1350 1360 1370 1380 1390 1400 AT1G64 GGGAAGAAGGAGTGGTATGTCCGATAATGGTGGAGGAATTGAACAGGGAAGGAGAAATGCAGATAGAGATGGTGTGTGAGGAAAGAGAGGAGTTCTTGGA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05H GGGAAGAAGGAGTGGTATGTCCGATAATGGTGGAGGAATTGAACAGGGAAGGAGAAATGCAGATAGAGATGGTGTGTGAGGAAAGAGAGGAGTTCTTGGA 1280 1290 1300 1310 1320 1330 1340 1350 1360 1370 1410 1420 1430 1440 1450 1460 1470 1480 1490 1500 AT1G64 GATAGGACAAGTGGTGAGAGGATTGGGATTGAAGATATTGAAAGGAGTGATGGAAACAAGAAAAGGACAAATATGGGCACACTTCATCGTACAGGCAAAG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05H GATAGGACAAGTGGTGAGAGGATTGGGATTGAAGATATTGAAAGGAGTGATGGAAACAAGAAAAGGACAAATATGGGCACACTTCATCGTACAGGCAAAG 1380 1390 1400 1410 1420 1430 1440 1450 1460 1470 1510 1520 1530 1540 1550 1560 1570 1580 AT1G64 CCACAAGTCACAAGGATTCAAGTCTTGTACTCACTTGTTCAGCTCTTTCAACACCACACCAAACATGATGATTTATTATCA ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05H CCACAAGTCACAAGGATTCAAGTCTTGTACTCACTTGTTCAGCTCTTTCAACACCACACCAAACATGATGATTTATTATCA 1480 1490 1500 1510 1520 1530 1540 1550 10 20 30 40 50 60 70 80 90 100 AT1G64 MGSEYKHILKSLCLSHGWSYAVFWRYDPINSMILRFEEAYNDEQSVALVDDMVLQAPILGQGIVGEVASSGNHQWLFSDTLFQWEHEFQNQFLCGFKILI ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05H MGSEYKHILKSLCLSHGWSYAVFWRYDPINSMILRFEEAYNDEQSVALVDDMVLQAPILGQGIVGEVASSGNHQWLFSDTLFQWEHEFQNQFLCGFK--- 10 20 30 40 50 60 70 80 90 110 120 130 140 150 160 170 180 190 200 AT1G64 RQFTYTQTIAIIPLGSSGVVQLGSTQKILESTEILEQTTRALQETCLKPHDSGDLDTLFESLGDCEIFPAESFQGFSFDDIFAEDNPPSLLSPEMISSEA ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05H -------TIAIIPLGSSGVVQLGSTQKILESTEILEQTTRALQETCLKPHDSGDLDTLFESLGDCEIFPAESFQGFSFDDIFAEDNPPSLLSPEMISSEA 100 110 120 130 140 150 160 170 180 190 210 220 230 240 250 260 270 280 290 300 AT1G64 ASSNQDLTNGDDYGFDILQSYSLDDLYQLLADPPEQNCSSMVIQGVDKDLFDILGMNSQTPTMALPPKGLFSELISSSLSNNTCSSSLTNVQEYSGVNQS :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05H ASSNQDLTNGDDYGFDILQSYSLDDLYQLLADPPEQNCSSMVIQGVDKDLFDILGMNSQTPTMALPPKGLFSELISSSLSNNTCSSSLTNVQEYSGVNQS 200 210 220 230 240 250 260 270 280 290 310 320 330 340 350 360 370 380 390 400 AT1G64 KRRKLDTSSAHSSSLFPQEETVTSRSLWIDDDERSSIGGNWKKPHEEGVKKKRAKAGESRRPRPKDRQMIQDRIKELRGMIPNGAKCSIDTLLDLTIKHM :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05H KRRKLDTSSAHSSSLFPQEETVTSRSLWIDDDERSSIGGNWKKPHEEGVKKKRAKAGESRRPRPKDRQMIQDRIKELRGMIPNGAKCSIDTLLDLTIKHM 300 310 320 330 340 350 360 370 380 390 410 420 430 440 450 460 470 480 490 500 AT1G64 VFMQSLAKYAERLKQPYESKLVKEKERTWALEVGEEGVVCPIMVEELNREGEMQIEMVCEEREEFLEIGQVVRGLGLKILKGVMETRKGQIWAHFIVQAK :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_05H VFMQSLAKYAERLKQPYESKLVKEKERTWALEVGEEGVVCPIMVEELNREGEMQIEMVCEEREEFLEIGQVVRGLGLKILKGVMETRKGQIWAHFIVQAK 400 410 420 430 440 450 460 470 480 490 510 520 AT1G64 PQVTRIQVLYSLVQLFQHHTKHDDLLS ::::::::::::::::::::::::::: IE_05H PQVTRIQVLYSLVQLFQHHTKHDDLLS 500 510

このページのTOPに戻る