◆◆◆IE_06A05（IE_09C04） detail◆◆◆

Code nameIE_06A05（IE_09C04）
LocusAT2G21900
TemplateIT_06A05 (ABRC_015)
Donor VectorpDONR207
Forward primerIPF_06A05 (ggggacaagtttgtacaaaaaagcaggcttcATGAACTATCCTTCAAACCCTAACCCT)
Reverse primerIPR_06A05 (ggggaccactttgtacaagaaagctgggttATATGGAGCAGAATGAGAGAAACTATACGT)
Alignment with template seq.1stアミノ酸配列100%一致2ndアミノ酸配列100.00%一致
Alignment with TAIR7CDSアミノ酸配列100%一致アミノ酸配列100.00%一致
comment1st:(Sequence解析まで行ったが、フレームはずれないが塩基置換がある)→2nd(09C04):70%, nt.23A→G, nt.408C→T
sequence 
>IE_06A05_606bp
ATGAACTATCCTTCAAACCCTAGCCCTAGCTCCACAGATTTCACTGAATTTTTCAAGTTCGATGATTTTGACGATACTTTTGAGAAGATCATGGAAGAAA
TCGGCCGTGAGGACCACTCGTCGTCACCGACTTTGAGTTGGAGTTCATCGGAAAAGTTAGTGGCTGCAGAAATCACAAGCCCGCTTCAAACAAGCCTAGC
TACCTCACCTATGAGCTTTGAAATAGGTGACAAAGATGAAATCAAAAAGAGGAAGAGACACAAAGAAGATCCGATTATTCACGTCTTCAAAACGAAATCA
TCAATTGATGAAAAGGTTGCTTTAGATGATGGGTATAAATGGAGGAAATACGGAAAGAAGCCGATAACGGGTAGTCCATTTCCAAGGCATTATCACAAGT
GTTCGAGTCCAGATTGCAACGTGAAGAAGAAGATCGAAAGAGATACGAACAATCCAGATTACATATTGACAACATACGAAGGTAGACATAACCACCCAAG
CCCTTCTGTAGTTTATTGTGATTCAGACGACTTTGATCTTAACTCTCTCAACAATTGGTCCTTTCAGACGGCAAATACGTATAGTTTCTCTCATTCTGCT
CCATAT

>IE_06A05_translated
MNYPSNPSPSSTDFTEFFKFDDFDDTFEKIMEEIGREDHSSSPTLSWSSSEKLVAAEITSPLQTSLATSPMSFEIGDKDEIKKRKRHKEDPIIHVFKTKS
SIDEKVALDDGYKWRKYGKKPITGSPFPRHYHKCSSPDCNVKKKIERDTNNPDYILTTYEGRHNHPSPSVVYCDSDDFDLNSLNNWSFQTANTYSFSHSA
PY
Alignment ◆CDS（TAIRver.9）_without_stop_codonとのalignment◆

604/606 (99.7%)（アミノ酸配列で201/202 (99.5%)）一致しました。


               10        20        30        40        50        60        70        80        90       100
AT2G21 ATGAACTATCCTTCAAACCCTAACCCTAGCTCCACAGATTTCACTGAATTTTTCAAGTTCGATGATTTTGACGATACTTTTGAGAAGATCATGGAAGAAA
       :::::::::::::::::::::: :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_06A ATGAACTATCCTTCAAACCCTAGCCCTAGCTCCACAGATTTCACTGAATTTTTCAAGTTCGATGATTTTGACGATACTTTTGAGAAGATCATGGAAGAAA
               10        20        30        40        50        60        70        80        90       100

              110       120       130       140       150       160       170       180       190       200
AT2G21 TCGGCCGTGAGGACCACTCGTCGTCACCGACTTTGAGTTGGAGTTCATCGGAAAAGTTAGTGGCTGCAGAAATCACAAGCCCGCTTCAAACAAGCCTAGC
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_06A TCGGCCGTGAGGACCACTCGTCGTCACCGACTTTGAGTTGGAGTTCATCGGAAAAGTTAGTGGCTGCAGAAATCACAAGCCCGCTTCAAACAAGCCTAGC
              110       120       130       140       150       160       170       180       190       200

              210       220       230       240       250       260       270       280       290       300
AT2G21 TACCTCACCTATGAGCTTTGAAATAGGTGACAAAGATGAAATCAAAAAGAGGAAGAGACACAAAGAAGATCCGATTATTCACGTCTTCAAAACGAAATCA
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_06A TACCTCACCTATGAGCTTTGAAATAGGTGACAAAGATGAAATCAAAAAGAGGAAGAGACACAAAGAAGATCCGATTATTCACGTCTTCAAAACGAAATCA
              210       220       230       240       250       260       270       280       290       300

              310       320       330       340       350       360       370       380       390       400
AT2G21 TCAATTGATGAAAAGGTTGCTTTAGATGATGGGTATAAATGGAGGAAATACGGAAAGAAGCCGATAACGGGTAGTCCATTTCCAAGGCATTATCACAAGT
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_06A TCAATTGATGAAAAGGTTGCTTTAGATGATGGGTATAAATGGAGGAAATACGGAAAGAAGCCGATAACGGGTAGTCCATTTCCAAGGCATTATCACAAGT
              310       320       330       340       350       360       370       380       390       400

              410       420       430       440       450       460       470       480       490       500
AT2G21 GTTCGAGCCCAGATTGCAACGTGAAGAAGAAGATCGAAAGAGATACGAACAATCCAGATTACATATTGACAACATACGAAGGTAGACATAACCACCCAAG
       ::::::: ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_06A GTTCGAGTCCAGATTGCAACGTGAAGAAGAAGATCGAAAGAGATACGAACAATCCAGATTACATATTGACAACATACGAAGGTAGACATAACCACCCAAG
              410       420       430       440       450       460       470       480       490       500

              510       520       530       540       550       560       570       580       590       600
AT2G21 CCCTTCTGTAGTTTATTGTGATTCAGACGACTTTGATCTTAACTCTCTCAACAATTGGTCCTTTCAGACGGCAAATACGTATAGTTTCTCTCATTCTGCT
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_06A CCCTTCTGTAGTTTATTGTGATTCAGACGACTTTGATCTTAACTCTCTCAACAATTGGTCCTTTCAGACGGCAAATACGTATAGTTTCTCTCATTCTGCT
              510       520       530       540       550       560       570       580       590       600

             
AT2G21 CCATAT
       ::::::
IE_06A CCATAT
             




               10        20        30        40        50        60        70        80        90       100
AT2G21 MNYPSNPNPSSTDFTEFFKFDDFDDTFEKIMEEIGREDHSSSPTLSWSSSEKLVAAEITSPLQTSLATSPMSFEIGDKDEIKKRKRHKEDPIIHVFKTKS
       :::::::.::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_06A MNYPSNPSPSSTDFTEFFKFDDFDDTFEKIMEEIGREDHSSSPTLSWSSSEKLVAAEITSPLQTSLATSPMSFEIGDKDEIKKRKRHKEDPIIHVFKTKS
               10        20        30        40        50        60        70        80        90       100

              110       120       130       140       150       160       170       180       190       200
AT2G21 SIDEKVALDDGYKWRKYGKKPITGSPFPRHYHKCSSPDCNVKKKIERDTNNPDYILTTYEGRHNHPSPSVVYCDSDDFDLNSLNNWSFQTANTYSFSHSA
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_06A SIDEKVALDDGYKWRKYGKKPITGSPFPRHYHKCSSPDCNVKKKIERDTNNPDYILTTYEGRHNHPSPSVVYCDSDDFDLNSLNNWSFQTANTYSFSHSA
              110       120       130       140       150       160       170       180       190       200

         
AT2G21 PY
       ::
IE_06A PY

このページのTOPに戻る