◆◆◆IE_06C01 detail◆◆◆

Code nameIE_06C01
LocusAT4G18890
TemplateIT_06C01 (ABRC_062)
Donor VectorpDONR207
Forward primerIPF_06C01 (ggggacaagtttgtacaaaaaagcaggcttcATGACGTCGGGGACTAGAACGCCGACT)
Reverse primerIPR_06C01 (ggggaccactttgtacaagaaagctgggttTCTGGTTCTTGAGTTTCCAAGTGTAAGTTC)
Alignment with template seq.1stアミノ酸配列100%一致2nd-
Alignment with TAIR7CDSアミノ酸配列100%一致-
comment
sequence
>IE_06C01_852bp
ATGACGTCGGGGACTAGAACGCCGACTTGGAAAGAGAGAGAGAACAACAAACGGCGAGAGCGGCGAAGACGAGCGATTGCGGCTAAGATCTTCGCAGGAC
TAAGGATTCATGGAAACTTCAAGCTCCCTAAACACTGCGACAACAATGAAGTCCTCAAAGCTTTATGCAATGAAGCTGGTTGGACTGTAGAAGACGACGG
AACTACTTACCGCAAGGGATGCAAACCAATGGATCGAATGGACCTCATGAATGGTTCTACTTCAGCTAGTCCATGCTCATCGTATCAACATAGCCCTCGT
GCTTCCTACAATCCAAGCCCTTCGTCTTCATCATTCCCGAGTCCTACAAACCCATTTGGTGATGCTAACTCACTAATCCCATGGCTCAAGAACCTCTCTT
CAAACTCACCTTCCAAGCTTCCCTTCTTCCATGGAAATTCAATAAGCGCTCCCGTGACTCCGCCATTGGCTCGAAGCCCTACTCGTGATCAAGTAACCAT
CCCTGACTCTGGATGGCTCTCAGGAATGCAAACTCCGCAGAGCGGACCGTCTTCTCCTACTTTCAGTTTAGTTTCAAGAAACCCGTTTTTCGACAAAGAG
GCTTTTAAAATGGGAGATTGTAATTCACCAATGTGGACTCCTGGACAAAGTGGAAACTGCTCTCCAGCTATTCCTGCTGGTGTTGATCAGAACTCTGATG
TGCCAATGGCTGATGGAATGACGGCTGAGTTTGCGTTTGGTTGTAACGCAATGGCTGCGAATGGAATGGTGAAGCCTTGGGAAGGAGAAAGGATACATGG
AGAATGTGTTTCAGATGATTTAGAACTTACACTTGGAAACTCAAGAACCAGA

>IE_06C01_translated
MTSGTRTPTWKERENNKRRERRRRAIAAKIFAGLRIHGNFKLPKHCDNNEVLKALCNEAGWTVEDDGTTYRKGCKPMDRMDLMNGSTSASPCSSYQHSPR
ASYNPSPSSSSFPSPTNPFGDANSLIPWLKNLSSNSPSKLPFFHGNSISAPVTPPLARSPTRDQVTIPDSGWLSGMQTPQSGPSSPTFSLVSRNPFFDKE
AFKMGDCNSPMWTPGQSGNCSPAIPAGVDQNSDVPMADGMTAEFAFGCNAMAANGMVKPWEGERIHGECVSDDLELTLGNSRTR
Alignment CDS(TAIRver.9)_without_stop_codonとのalignment

100%一致しました。

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