◆◆◆IE_06C07 detail◆◆◆

Code nameIE_06C07
LocusAT3G55560
TemplateIT_06C07 (ABRC_071)
Donor VectorpDONR207
Forward primerIPF_06C07 (ggggacaagtttgtacaaaaaagcaggcttcATGGCGAATCCTTGGTGGGTAGGGAAT)
Reverse primerIPR_06C07 (ggggaccactttgtacaagaaagctgggttATACGAAGGAGGAGCACGAGGCGGAGGACC)
Alignment with template seq.1stアミノ酸配列100%一致2ndアミノ酸配列100%一致
Alignment with TAIR7CDSアミノ酸配列100%一致アミノ酸配列100%一致
comment1st:(Sequence解析まで行ったが、フレームはずれないが塩基置換がある), 2nd:70%, nt.567T→C
sequence 
>IE_06C07_930bp
ATGGCGAATCCTTGGTGGGTAGGGAATGTTGCGATCGGTGGAGTTGAGAGTCCAGTGACGTCATCAGCTCCTTCTTTGCACCACAGAAACAGTAACAACA
ACAACCCACCGACTATGACTCGTTCGGATCCAAGATTGGACCATGACTTCACCACCAACAACAGTGGAAGCCCTAATACCCAGACTCAGAGCCAAGAAGA
ACAGAACAGCAGAGACGAGCAACCAGCTGTTGAACCCGGATCCGGATCCGGGTCTACGGGTCGTCGTCCTAGAGGTAGACCTCCTGGTTCCAAGAACAAA
CCAAAGAGTCCAGTTGTTGTTACCAAAGAAAGCCCTAACTCTCTCCAGAGCCATGTTCTTGAGATTGCTACGGGAGCTGACGTGGCGGAAAGCTTAAACG
CCTTTGCTCGTAGACGCGGCCGGGGCGTTTCGGTGCTGAGCGGTAGTGGTTTGGTTACTAATGTTACTCTGCGTCAGCCTGCTGCATCCGGTGGAGTTGT
TAGTTTACGTGGTCAGTTTGAGATCTTGTCTATGTGTGGGGCTTTTCTTCCTACGTCTGGCTCTCCCGCTGCAGCCGCTGGTTTAACCATTTACTTAGCT
GGAGCTCAAGGTCAAGTTGTGGGAGGTGGAGTTGCTGGCCCGCTTATTGCCTCTGGACCCGTTATTGTGATAGCTGCTACGTTTTGCAATGCCACTTATG
AGAGGTTACCGATTGAGGAAGAACAACAGCAAGAGCAGCCGCTTCAACTAGAAGATGGGAAGAAGCAGAAAGAAGAGAATGATGATAACGAGAGTGGGAA
TAACGGAAACGAAGGATCGATGCAGCCGCCGATGTATAATATGCCTCCTAATTTTATCCCAAATGGTCATCAAATGGCTCAACACGACGTGTATTGGGGT
GGTCCTCCGCCTCGTGCTCCTCCTTCGTAT

>IE_06C07_translated
MANPWWVGNVAIGGVESPVTSSAPSLHHRNSNNNNPPTMTRSDPRLDHDFTTNNSGSPNTQTQSQEEQNSRDEQPAVEPGSGSGSTGRRPRGRPPGSKNK
PKSPVVVTKESPNSLQSHVLEIATGADVAESLNAFARRRGRGVSVLSGSGLVTNVTLRQPAASGGVVSLRGQFEILSMCGAFLPTSGSPAAAAGLTIYLA
GAQGQVVGGGVAGPLIASGPVIVIAATFCNATYERLPIEEEQQQEQPLQLEDGKKQKEENDDNESGNNGNEGSMQPPMYNMPPNFIPNGHQMAQHDVYWG
GPPPRAPPSY
Alignment ◆CDS（TAIRver.9）_without_stop_codonとのalignment◆

929/930 (99.9%)（アミノ酸配列で100%）一致しました。


               10        20        30        40        50        60        70        80        90       100
AT3G55 ATGGCGAATCCTTGGTGGGTAGGGAATGTTGCGATCGGTGGAGTTGAGAGTCCAGTGACGTCATCAGCTCCTTCTTTGCACCACAGAAACAGTAACAACA
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_06C ATGGCGAATCCTTGGTGGGTAGGGAATGTTGCGATCGGTGGAGTTGAGAGTCCAGTGACGTCATCAGCTCCTTCTTTGCACCACAGAAACAGTAACAACA
               10        20        30        40        50        60        70        80        90       100

              110       120       130       140       150       160       170       180       190       200
AT3G55 ACAACCCACCGACTATGACTCGTTCGGATCCAAGATTGGACCATGACTTCACCACCAACAACAGTGGAAGCCCTAATACCCAGACTCAGAGCCAAGAAGA
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_06C ACAACCCACCGACTATGACTCGTTCGGATCCAAGATTGGACCATGACTTCACCACCAACAACAGTGGAAGCCCTAATACCCAGACTCAGAGCCAAGAAGA
              110       120       130       140       150       160       170       180       190       200

              210       220       230       240       250       260       270       280       290       300
AT3G55 ACAGAACAGCAGAGACGAGCAACCAGCTGTTGAACCCGGATCCGGATCCGGGTCTACGGGTCGTCGTCCTAGAGGTAGACCTCCTGGTTCCAAGAACAAA
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_06C ACAGAACAGCAGAGACGAGCAACCAGCTGTTGAACCCGGATCCGGATCCGGGTCTACGGGTCGTCGTCCTAGAGGTAGACCTCCTGGTTCCAAGAACAAA
              210       220       230       240       250       260       270       280       290       300

              310       320       330       340       350       360       370       380       390       400
AT3G55 CCAAAGAGTCCAGTTGTTGTTACCAAAGAAAGCCCTAACTCTCTCCAGAGCCATGTTCTTGAGATTGCTACGGGAGCTGACGTGGCGGAAAGCTTAAACG
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_06C CCAAAGAGTCCAGTTGTTGTTACCAAAGAAAGCCCTAACTCTCTCCAGAGCCATGTTCTTGAGATTGCTACGGGAGCTGACGTGGCGGAAAGCTTAAACG
              310       320       330       340       350       360       370       380       390       400

              410       420       430       440       450       460       470       480       490       500
AT3G55 CCTTTGCTCGTAGACGCGGCCGGGGCGTTTCGGTGCTGAGCGGTAGTGGTTTGGTTACTAATGTTACTCTGCGTCAGCCTGCTGCATCCGGTGGAGTTGT
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_06C CCTTTGCTCGTAGACGCGGCCGGGGCGTTTCGGTGCTGAGCGGTAGTGGTTTGGTTACTAATGTTACTCTGCGTCAGCCTGCTGCATCCGGTGGAGTTGT
              410       420       430       440       450       460       470       480       490       500

              510       520       530       540       550       560       570       580       590       600
AT3G55 TAGTTTACGTGGTCAGTTTGAGATCTTGTCTATGTGTGGGGCTTTTCTTCCTACGTCTGGCTCTCCTGCTGCAGCCGCTGGTTTAACCATTTACTTAGCT
       :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: :::::::::::::::::::::::::::::::::
IE_06C TAGTTTACGTGGTCAGTTTGAGATCTTGTCTATGTGTGGGGCTTTTCTTCCTACGTCTGGCTCTCCCGCTGCAGCCGCTGGTTTAACCATTTACTTAGCT
              510       520       530       540       550       560       570       580       590       600

              610       620       630       640       650       660       670       680       690       700
AT3G55 GGAGCTCAAGGTCAAGTTGTGGGAGGTGGAGTTGCTGGCCCGCTTATTGCCTCTGGACCCGTTATTGTGATAGCTGCTACGTTTTGCAATGCCACTTATG
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_06C GGAGCTCAAGGTCAAGTTGTGGGAGGTGGAGTTGCTGGCCCGCTTATTGCCTCTGGACCCGTTATTGTGATAGCTGCTACGTTTTGCAATGCCACTTATG
              610       620       630       640       650       660       670       680       690       700

              710       720       730       740       750       760       770       780       790       800
AT3G55 AGAGGTTACCGATTGAGGAAGAACAACAGCAAGAGCAGCCGCTTCAACTAGAAGATGGGAAGAAGCAGAAAGAAGAGAATGATGATAACGAGAGTGGGAA
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_06C AGAGGTTACCGATTGAGGAAGAACAACAGCAAGAGCAGCCGCTTCAACTAGAAGATGGGAAGAAGCAGAAAGAAGAGAATGATGATAACGAGAGTGGGAA
              710       720       730       740       750       760       770       780       790       800

              810       820       830       840       850       860       870       880       890       900
AT3G55 TAACGGAAACGAAGGATCGATGCAGCCGCCGATGTATAATATGCCTCCTAATTTTATCCCAAATGGTCATCAAATGGCTCAACACGACGTGTATTGGGGT
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_06C TAACGGAAACGAAGGATCGATGCAGCCGCCGATGTATAATATGCCTCCTAATTTTATCCCAAATGGTCATCAAATGGCTCAACACGACGTGTATTGGGGT
              810       820       830       840       850       860       870       880       890       900

              910       920       930
AT3G55 GGTCCTCCGCCTCGTGCTCCTCCTTCGTAT
       ::::::::::::::::::::::::::::::
IE_06C GGTCCTCCGCCTCGTGCTCCTCCTTCGTAT
              910       920       930




               10        20        30        40        50        60        70        80        90       100
AT3G55 MANPWWVGNVAIGGVESPVTSSAPSLHHRNSNNNNPPTMTRSDPRLDHDFTTNNSGSPNTQTQSQEEQNSRDEQPAVEPGSGSGSTGRRPRGRPPGSKNK
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_06C MANPWWVGNVAIGGVESPVTSSAPSLHHRNSNNNNPPTMTRSDPRLDHDFTTNNSGSPNTQTQSQEEQNSRDEQPAVEPGSGSGSTGRRPRGRPPGSKNK
               10        20        30        40        50        60        70        80        90       100

              110       120       130       140       150       160       170       180       190       200
AT3G55 PKSPVVVTKESPNSLQSHVLEIATGADVAESLNAFARRRGRGVSVLSGSGLVTNVTLRQPAASGGVVSLRGQFEILSMCGAFLPTSGSPAAAAGLTIYLA
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_06C PKSPVVVTKESPNSLQSHVLEIATGADVAESLNAFARRRGRGVSVLSGSGLVTNVTLRQPAASGGVVSLRGQFEILSMCGAFLPTSGSPAAAAGLTIYLA
              110       120       130       140       150       160       170       180       190       200

              210       220       230       240       250       260       270       280       290       300
AT3G55 GAQGQVVGGGVAGPLIASGPVIVIAATFCNATYERLPIEEEQQQEQPLQLEDGKKQKEENDDNESGNNGNEGSMQPPMYNMPPNFIPNGHQMAQHDVYWG
       ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_06C GAQGQVVGGGVAGPLIASGPVIVIAATFCNATYERLPIEEEQQQEQPLQLEDGKKQKEENDDNESGNNGNEGSMQPPMYNMPPNFIPNGHQMAQHDVYWG
              210       220       230       240       250       260       270       280       290       300

              310
AT3G55 GPPPRAPPSY
       ::::::::::
IE_06C GPPPRAPPSY
              310

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