◆◆◆IE_06C08（IE_09C07） detail◆◆◆

Code nameIE_06C08（IE_09C07）
LocusAT4G12050
TemplateIT_06C08 (ABRC_072)
Donor VectorpDONR207
Forward primerIPF_06C08 (ggggacaagtttgtacaaaaaagcaggcttcATGGATCCAGTTCAATCTCATGGATCA)
Reverse primerIPR_06C08 (ggggaccactttgtacaagaaagctgggttATACGGTGGCCGACCCGTAGACCAATACTG)
Alignment with template seq.1st配列解析が行えなかったサンプルです。2ndアミノ酸配列100%一致
Alignment with TAIR7CDS-アミノ酸配列100%一致
comment1st:(Sequence解析まで行ったが、読み直す必要がある)→2nd(09C07):100%
sequence 
>IE_06C08_1017bp
ATGGATCCAGTTCAATCTCATGGATCACAAAGCTCTCTTCCTCCTCCTTTCCATGCTAGAGATTTCCAATTACATCTTCAACAACAACAACAACATCAAC
AACAACATCAACAACAACAACAACAACAGTTCTTTCTCCACCATCATCAGCAACCACAAAGAAACCTTGATCAAGATCACGAGCAGCAAGGAGGGTCAAT
ATTGAATAGATCTATCAAGATGGATCGCGAAGAGACAAGCGATAACATGGACAACATCGCTAATACCAACAGCGGTAGCGAAGGTAAAGAGATGAGTTTA
CACGGAGGAGAAGGAGGAAGCGGTGGTGGAGGAAGTGGAGAACAGATGACAAGAAGGCCAAGAGGAAGACCAGCAGGATCCAAGAACAAACCTAAAGCTC
CAATAATCATAACAAGAGACAGCGCAAACGCGCTTCGAACTCACGTCATGGAGATAGGAGACGGATGTGACATAGTTGACTGTATGGCTACGTTCGCTAG
ACGCCGCCAAAGAGGCGTTTGCGTTATGAGCGGTACAGGAAGCGTTACTAACGTCACTATACGTCAGCCTGGATCGCCACCTGGCTCGGTGGTTAGCCTT
CACGGCCGGTTTGAAATCCTCTCTCTTTCGGGATCTTTCTTGCCTCCGCCTGCGCCGCCTGCAGCCACCGGACTAAGCGTTTACCTAGCCGGAGGACAAG
GGCAGGTCGTTGGAGGTAGTGTGGTGGGACCTTTGTTGTGTTCGGGTCCTGTGGTGGTTATGGCGGCTTCTTTTAGCAATGCGGCGTACGAAAGGCTGCC
TTTGGAAGAAGATGAGATGCAGACGCCAGTTCAAGGAGGCGGTGGAGGAGGAGGAGGTGGTGGTGGAATGGGATCTCCCCCGATGATGGGACAGCAACAA
GCTATGGCAGCTATGGCGGCGGCTCAAGGACTACCACCGAATCTTCTTGGTTCGGTTCAGTTGCCACCGCCACAACAGAATGATCAGCAGTATTGGTCTA
CGGGTCGGCCACCGTAT

>IE_06C08_translated
MDPVQSHGSQSSLPPPFHARDFQLHLQQQQQHQQQHQQQQQQQFFLHHHQQPQRNLDQDHEQQGGSILNRSIKMDREETSDNMDNIANTNSGSEGKEMSL
HGGEGGSGGGGSGEQMTRRPRGRPAGSKNKPKAPIIITRDSANALRTHVMEIGDGCDIVDCMATFARRRQRGVCVMSGTGSVTNVTIRQPGSPPGSVVSL
HGRFEILSLSGSFLPPPAPPAATGLSVYLAGGQGQVVGGSVVGPLLCSGPVVVMAASFSNAAYERLPLEEDEMQTPVQGGGGGGGGGGGMGSPPMMGQQQ
AMAAMAAAQGLPPNLLGSVQLPPPQQNDQQYWSTGRPPY
Alignment ◆CDS（TAIRver.9）_without_stop_codonとのalignment◆

100%一致しました。

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