◆◆◆IE_06C10 detail◆◆◆

Code name	IE_06C10
Locus	AT2G22750
Template	IT_06C10 (ABRC_074)
Donor Vector	pDONR207
Forward primer	IPF_06C10 (ggggacaagtttgtacaaaaaagcaggcttcATGAACTCACTCGTCGGAGATGTTCCA)
Reverse primer	IPR_06C10 (ggggaccactttgtacaagaaagctgggttAGTGAGCTTTGATAAGCCAAAACTCAAGTT)
Alignment with template seq.	1st	アミノ酸配列99.70%一致	2nd	アミノ酸配列99.70%一致
Alignment with TAIR7CDS	1st	アミノ酸配列99.70%一致	2nd	アミノ酸配列99.70%一致
comment	1st:(Sequence解析まで行ったが、読み直す必要がある?), 2nd:70%, 3nt_ins
sequence	>IE_06C10_915bp ATGAACTCACTCGTCGGAGATGTTCCACAGTCTCTCTCATCTCTTGATGATACCACCACTTGTTATAACCTTGATGCTTCTTGTAATAAAAGTTTGGTAG AAGAAAGACCTTCAAAGATCCTCAAGACCACTCACATATCACCAAACTTACATCCTTTTTCTTCTTCTAATCCTCCTCCTCCAAAGCACCAGCCCTCTTC TAGGATTCTTTCTTTTGAAAAGACAGGTTTACATGTTATGAATCACAACTCTCCAAACTTAATATTTAGCCCCAAGGACGAAGAAATTGGATTACCAGAG CATAAGAAAGCCGAGCTGATAATAAGAGGGACAAAGAGAGCTCAATCCTTGACTCGAAGCCAATCAAATGCTCAAGATCACATACTGGCAGAGAGAAAAC GGAGAGAGAAGCTTACTCAAAGATTTGTAGCTCTTTCCGCGCTAATTCCTGGCCTAAAGAAGATGGACAAGGCTTCTGTGTTGGGAGATGCAATAAAGCA TATAAAGTACCTCCAAGAGAGTGTGAAAGAGTATGAGGAACAAAAGAAGGAAAAGACAATGGAATCAGTGGTTCTTGTAAAGAAGTCTAGTCTGGTTTTA GATGAAAATCATCAACCATCATCATCATCTTCCTCAGATGGAAATCGCAATAGCTCGAGCTCAAATCTTCCAGAAATAGAAGTTAGGGTTTCAGGAAAAG ATGTTCTTATTAAGATCCTATGCGAGAAGCAAAAGGGTAATGTGATCAAGATTATGGGGGAGATTGAAAAGCTTGGTTTGTCTATCACCAACAGCAATGT CTTGCCCTTTGGACCCACTTTTGACATCTCTATTATCGCTCAGAAGAATAACAATTTTGATATGAAAATCGAGGATGTTGTGAAGAACTTGAGTTTTGGC TTATCAAAGCTCACT >IE_06C10_translated MNSLVGDVPQSLSSLDDTTTCYNLDASCNKSLVEERPSKILKTTHISPNLHPFSSSNPPPPKHQPSSRILSFEKTGLHVMNHNSPNLIFSPKDEEIGLPE HKKAELIIRGTKRAQSLTRSQSNAQDHILAERKRREKLTQRFVALSALIPGLKKMDKASVLGDAIKHIKYLQESVKEYEEQKKEKTMESVVLVKKSSLVL DENHQPSSSSSSDGNRNSSSSNLPEIEVRVSGKDVLIKILCEKQKGNVIKIMGEIEKLGLSITNSNVLPFGPTFDISIIAQKNNNFDMKIEDVVKNLSFG LSKLT
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 912/915 (99.7%)（アミノ酸配列で304/305 (99.7%)）一致しました。 10 20 30 40 50 60 70 80 90 100 AT2G22 ATGAACTCACTCGTCGGAGATGTTCCACAGTCTCTCTCATCTCTTGATGATACCACCACTTGTTATAACCTTGATGCTTCTTGTAATAAAAGTTTGGTAG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_06C ATGAACTCACTCGTCGGAGATGTTCCACAGTCTCTCTCATCTCTTGATGATACCACCACTTGTTATAACCTTGATGCTTCTTGTAATAAAAGTTTGGTAG 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT2G22 AAGAAAGACCTTCAAAGATCCTCAAGACCACTCACATATCACCAAACTTACATCCTTTTTCTTCTTCTAATCCTCCTCCTCCAAAGCACCAGCCCTCTTC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_06C AAGAAAGACCTTCAAAGATCCTCAAGACCACTCACATATCACCAAACTTACATCCTTTTTCTTCTTCTAATCCTCCTCCTCCAAAGCACCAGCCCTCTTC 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT2G22 TAGGATTCTTTCTTTTGAAAAGACAGGTTTACATGTTATGAATCACAACTCTCCAAACTTAATATTTAGCCCCAAGGACGAAGAAATTGGATTACCAGAG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_06C TAGGATTCTTTCTTTTGAAAAGACAGGTTTACATGTTATGAATCACAACTCTCCAAACTTAATATTTAGCCCCAAGGACGAAGAAATTGGATTACCAGAG 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 AT2G22 CATAAGAAAGCCGAGCTGATAATAAGAGGGACAAAGAGAGCTCAATCCTTGACTCGAAGCCAATCAAATGCTCAAGATCACATACTGGCAGAGAGAAAAC :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_06C CATAAGAAAGCCGAGCTGATAATAAGAGGGACAAAGAGAGCTCAATCCTTGACTCGAAGCCAATCAAATGCTCAAGATCACATACTGGCAGAGAGAAAAC 310 320 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 480 490 500 AT2G22 GGAGAGAGAAGCTTACTCAAAGATTTGTAGCTCTTTCCGCGCTAATTCCTGGCCTAAAGAAGATGGACAAGGCTTCTGTGTTGGGAGATGCAATAAAGCA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_06C GGAGAGAGAAGCTTACTCAAAGATTTGTAGCTCTTTCCGCGCTAATTCCTGGCCTAAAGAAGATGGACAAGGCTTCTGTGTTGGGAGATGCAATAAAGCA 410 420 430 440 450 460 470 480 490 500 510 520 530 540 550 560 570 580 590 600 AT2G22 TATAAAGTACCTCCAAGAGAGTGTGAAAGAGTATGAGGAACAAAAGAAGGAAAAGACAATGGAATCAGTGGTTCTTGTAAAGAAGTCTAGTCTGGTTTTA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_06C TATAAAGTACCTCCAAGAGAGTGTGAAAGAGTATGAGGAACAAAAGAAGGAAAAGACAATGGAATCAGTGGTTCTTGTAAAGAAGTCTAGTCTGGTTTTA 510 520 530 540 550 560 570 580 590 600 610 620 630 640 650 660 670 680 690 700 AT2G22 GATGAAAATCATCAACCATCATCATCATCTTCCTCAGATGGAAATCGCAATAGCTCGAGCTCAAATCTTCCAGAAATAGAAGTTAGGGTTTCAGGAAAAG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_06C GATGAAAATCATCAACCATCATCATCATCTTCCTCAGATGGAAATCGCAATAGCTCGAGCTCAAATCTTCCAGAAATAGAAGTTAGGGTTTCAGGAAAAG 610 620 630 640 650 660 670 680 690 700 710 720 730 740 750 760 770 780 790 800 AT2G22 ATGTTCTTATTAAGATCCTATGCGAGAAGCAAAAGGGTAATGTGATCAAGATTATGGGGGAGATTGAAAAGCTTGGTTTGTCTATCACCAACAGCAATGT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_06C ATGTTCTTATTAAGATCCTATGCGAGAAGCAAAAGGGTAATGTGATCAAGATTATGGGGGAGATTGAAAAGCTTGGTTTGTCTATCACCAACAGCAATGT 710 720 730 740 750 760 770 780 790 800 810 820 830 840 850 860 870 880 890 AT2G22 CTTGCCCTTTGGACCCACTTTTGACATCTCTATTATCGCTC---AGAATAACAATTTTGATATGAAAATCGAGGATGTTGTGAAGAACTTGAGTTTTGGC ::::::::::::::::::::::::::::::::::::::::: :::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_06C CTTGCCCTTTGGACCCACTTTTGACATCTCTATTATCGCTCAGAAGAATAACAATTTTGATATGAAAATCGAGGATGTTGTGAAGAACTTGAGTTTTGGC 810 820 830 840 850 860 870 880 890 900 900 910 AT2G22 TTATCAAAGCTCACT ::::::::::::::: IE_06C TTATCAAAGCTCACT 910 10 20 30 40 50 60 70 80 90 100 AT2G22 MNSLVGDVPQSLSSLDDTTTCYNLDASCNKSLVEERPSKILKTTHISPNLHPFSSSNPPPPKHQPSSRILSFEKTGLHVMNHNSPNLIFSPKDEEIGLPE :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_06C MNSLVGDVPQSLSSLDDTTTCYNLDASCNKSLVEERPSKILKTTHISPNLHPFSSSNPPPPKHQPSSRILSFEKTGLHVMNHNSPNLIFSPKDEEIGLPE 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT2G22 HKKAELIIRGTKRAQSLTRSQSNAQDHILAERKRREKLTQRFVALSALIPGLKKMDKASVLGDAIKHIKYLQESVKEYEEQKKEKTMESVVLVKKSSLVL :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_06C HKKAELIIRGTKRAQSLTRSQSNAQDHILAERKRREKLTQRFVALSALIPGLKKMDKASVLGDAIKHIKYLQESVKEYEEQKKEKTMESVVLVKKSSLVL 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 AT2G22 DENHQPSSSSSSDGNRNSSSSNLPEIEVRVSGKDVLIKILCEKQKGNVIKIMGEIEKLGLSITNSNVLPFGPTFDISIIAQ-NNNFDMKIEDVVKNLSFG ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: :::::::::::::::::: IE_06C DENHQPSSSSSSDGNRNSSSSNLPEIEVRVSGKDVLIKILCEKQKGNVIKIMGEIEKLGLSITNSNVLPFGPTFDISIIAQKNNNFDMKIEDVVKNLSFG 210 220 230 240 250 260 270 280 290 300 300 AT2G22 LSKLT ::::: IE_06C LSKLT

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