◆◆◆IE_06D08 detail◆◆◆

Code name	IE_06D08
Locus	AT2G18500
Template	IT_06D08 (ABRC_095)
Donor Vector	pDONR207
Forward primer	IPF_06D08 (ggggacaagtttgtacaaaaaagcaggcttcATGACGAAACGTTTCAAATTAAAGATT)
Reverse primer	IPR_06D08 (ggggaccactttgtacaagaaagctgggttACACTCATCGTAGTCTGAGAGTCGAACACT)
Alignment with template seq.	1st	アミノ酸配列99.70%一致	2nd	-
Alignment with TAIR7CDS	1st	アミノ酸配列99.70%一致	2nd	-
comment	1st:70%
sequence	>IE_06D08_945bp ATGACGAAACGTTTCAAATTAAAGATTTCAAGAATCCTCTCATTCAAATCTTGCCGTTTAAAAGATCCTTCTTCTCTTCCTTTTAATCCTGTTTCTTCAA GTCTCCGACGTACTTCTCCACTGGTTAACTCCTCCGCCGACGTCACCACCGTGCCGCAACGTCGCCGTTCTTCTTTCAGACTACACGTTTTAACTGTGTT TGGCTGCGGACGCTCGTCCACGCCGTTGGATGTTGATCTGAGAAACTCACCGGTGTTATCTCCGCCGCAAACGCCGACGTTTCAGTGGGAAAGTGAAGGG AAGTGGCACGTGATTGCTCAAGTCACTGAAGAAGAATACGAAACGCCTCGTCGGAAAATATACAACGGTGGGTCGGAGAAAGATAATCGCCGGCGTTTAA AGAAGAAGGAGAAATCAAACTCTCGGTGGCGAGGGAGCATTTCCTCCGCCGAAGAAGAGACTGATAGAGAGAGTCTCTTACCATCTTCTACAAACCTCTC GCCGGAATATTCCTCTTCCGAGCTACCGCGTGTCACAAGACGTCCGAGACAACTTCTCAAGAAGGCGGTGATCGAGGAAGAAAGTGAATCATCATCACCG CCACCGTCTCCGGCGAGACTGTCTTCGTTTGTGCAGAGATTAATGCCGTGTACGATGGCGGCGGCGGTTATGGTGGAAGGAGTGGCGGTGGTGAAGAAAT CGGAGGATCCATACGAAGATTTTAAAGGATCAATGATGGAGATGATAGTAGAGAAGAAGATGTTTGAAGTAGCTGAGCTTGAACAGCTTCTTAGTTGCTT CCTATCGCTAAACGCGAAACGCCATCACCGCGCGATTGTAAGAGCGTTTTCAGAGATTTGGGTTGCTTTGTTCTCCGGTGGTAGTGGTGGTGGTCGGAGA TCGTCCTCCTTCTCGAGTGTTCGACTCTCAGACTACGATGAGTGT >IE_06D08_translated MTKRFKLKISRILSFKSCRLKDPSSLPFNPVSSSLRRTSPLVNSSADVTTVPQRRRSSFRLHVLTVFGCGRSSTPLDVDLRNSPVLSPPQTPTFQWESEG KWHVIAQVTEEEYETPRRKIYNGGSEKDNRRRLKKKEKSNSRWRGSISSAEEETDRESLLPSSTNLSPEYSSSELPRVTRRPRQLLKKAVIEEESESSSP PPSPARLSSFVQRLMPCTMAAAVMVEGVAVVKKSEDPYEDFKGSMMEMIVEKKMFEVAELEQLLSCFLSLNAKRHHRAIVRAFSEIWVALFSGGSGGGRR SSSFSSVRLSDYDEC
Alignment	◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 943/945 (99.8%)（アミノ酸配列で314/315 (99.7%)）一致しました。 10 20 30 40 50 60 70 80 90 100 AT2G18 ATGACGAAACGTTTCAAATTAAAGATTTCAAGAATCCTCTCATTCAAATCTTGCCGTTTAAAAGATCCTTCTTCTCTTCCTTTTAATCCTGTTTCTTCAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_06D ATGACGAAACGTTTCAAATTAAAGATTTCAAGAATCCTCTCATTCAAATCTTGCCGTTTAAAAGATCCTTCTTCTCTTCCTTTTAATCCTGTTTCTTCAA 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT2G18 GTCTCCGACGTACTTCTCCACTGGTTAACTCCTCCGCCGACGTCACCACCGTGCCGCAACGTCGCCGTTCTTCTTTCAGACTACACGTTTTAACTGTGTT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_06D GTCTCCGACGTACTTCTCCACTGGTTAACTCCTCCGCCGACGTCACCACCGTGCCGCAACGTCGCCGTTCTTCTTTCAGACTACACGTTTTAACTGTGTT 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT2G18 TGGCTGCGGACGCTCGTCCACGCCGTTGGATGTTGATCTGAGAAACTCACCGGTGTTATCTCCGCCGCAAACGCCGACGTTTCAGTGGGAAAGTGAAGGG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_06D TGGCTGCGGACGCTCGTCCACGCCGTTGGATGTTGATCTGAGAAACTCACCGGTGTTATCTCCGCCGCAAACGCCGACGTTTCAGTGGGAAAGTGAAGGG 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 AT2G18 AAGTGGCACGTGATTGCTCAAGTCACTGAAGAAGAATACGAAACGCCTCGTCGGAAAATATACAACGGTGGGTCGGAGAAAGATAATCGCCGGCGTTTAA :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_06D AAGTGGCACGTGATTGCTCAAGTCACTGAAGAAGAATACGAAACGCCTCGTCGGAAAATATACAACGGTGGGTCGGAGAAAGATAATCGCCGGCGTTTAA 310 320 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 480 490 500 AT2G18 AGAAGAAGGAGAAATCAAACTCTCGGCGGCGAGGGAGCATTTCCTCCGCCGAAGAAGAGACTGATAGAGAGAGTCTCTTACCATCTTCTACAAACCTCTC :::::::::::::::::::::::::: ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_06D AGAAGAAGGAGAAATCAAACTCTCGGTGGCGAGGGAGCATTTCCTCCGCCGAAGAAGAGACTGATAGAGAGAGTCTCTTACCATCTTCTACAAACCTCTC 410 420 430 440 450 460 470 480 490 500 510 520 530 540 550 560 570 580 590 600 AT2G18 GCCGGAATATTCCTCTTCCGAGCTACCGCGTGTCACAAGACGTCCGAGACAACTTCTCAAGAAGGCGGTGATCGAGGAAGAAAGTGAATCATCATCACCG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_06D GCCGGAATATTCCTCTTCCGAGCTACCGCGTGTCACAAGACGTCCGAGACAACTTCTCAAGAAGGCGGTGATCGAGGAAGAAAGTGAATCATCATCACCG 510 520 530 540 550 560 570 580 590 600 610 620 630 640 650 660 670 680 690 700 AT2G18 CCACCGTCTCCGGCGAGACTGTCTTCGTTTGTGCAGAGATTAATGCCGTGTACGATGGCGGCGGCGGTTATGGTGGAAGGAGTGGCGGTGGTGAAGAAAT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_06D CCACCGTCTCCGGCGAGACTGTCTTCGTTTGTGCAGAGATTAATGCCGTGTACGATGGCGGCGGCGGTTATGGTGGAAGGAGTGGCGGTGGTGAAGAAAT 610 620 630 640 650 660 670 680 690 700 710 720 730 740 750 760 770 780 790 800 AT2G18 CGGAGGATCCATACGAAGATTTTAAAGGATCAATGATGGAGATGATAGTAGAGAAGAAGATGTTTGAAGTAGCTGAGCTTGAACAGCTTCTTAGTTGCTT :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_06D CGGAGGATCCATACGAAGATTTTAAAGGATCAATGATGGAGATGATAGTAGAGAAGAAGATGTTTGAAGTAGCTGAGCTTGAACAGCTTCTTAGTTGCTT 710 720 730 740 750 760 770 780 790 800 810 820 830 840 850 860 870 880 890 900 AT2G18 CCTATCGCTAAACGCGAAACGCCACCACCGCGCGATTGTAAGAGCGTTTTCAGAGATTTGGGTTGCTTTGTTCTCCGGTGGTAGTGGTGGTGGTCGGAGA :::::::::::::::::::::::: ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_06D CCTATCGCTAAACGCGAAACGCCATCACCGCGCGATTGTAAGAGCGTTTTCAGAGATTTGGGTTGCTTTGTTCTCCGGTGGTAGTGGTGGTGGTCGGAGA 810 820 830 840 850 860 870 880 890 900 910 920 930 940 AT2G18 TCGTCCTCCTTCTCGAGTGTTCGACTCTCAGACTACGATGAGTGT ::::::::::::::::::::::::::::::::::::::::::::: IE_06D TCGTCCTCCTTCTCGAGTGTTCGACTCTCAGACTACGATGAGTGT 910 920 930 940 10 20 30 40 50 60 70 80 90 100 AT2G18 MTKRFKLKISRILSFKSCRLKDPSSLPFNPVSSSLRRTSPLVNSSADVTTVPQRRRSSFRLHVLTVFGCGRSSTPLDVDLRNSPVLSPPQTPTFQWESEG :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_06D MTKRFKLKISRILSFKSCRLKDPSSLPFNPVSSSLRRTSPLVNSSADVTTVPQRRRSSFRLHVLTVFGCGRSSTPLDVDLRNSPVLSPPQTPTFQWESEG 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 AT2G18 KWHVIAQVTEEEYETPRRKIYNGGSEKDNRRRLKKKEKSNSRRRGSISSAEEETDRESLLPSSTNLSPEYSSSELPRVTRRPRQLLKKAVIEEESESSSP :::::::::::::::::::::::::::::::::::::::::: ::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_06D KWHVIAQVTEEEYETPRRKIYNGGSEKDNRRRLKKKEKSNSRWRGSISSAEEETDRESLLPSSTNLSPEYSSSELPRVTRRPRQLLKKAVIEEESESSSP 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 AT2G18 PPSPARLSSFVQRLMPCTMAAAVMVEGVAVVKKSEDPYEDFKGSMMEMIVEKKMFEVAELEQLLSCFLSLNAKRHHRAIVRAFSEIWVALFSGGSGGGRR :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: IE_06D PPSPARLSSFVQRLMPCTMAAAVMVEGVAVVKKSEDPYEDFKGSMMEMIVEKKMFEVAELEQLLSCFLSLNAKRHHRAIVRAFSEIWVALFSGGSGGGRR 210 220 230 240 250 260 270 280 290 300 310 AT2G18 SSSFSSVRLSDYDEC ::::::::::::::: IE_06D SSSFSSVRLSDYDEC 310

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