◆◆◆IE_06F06 detail◆◆◆

Code nameIE_06F06
LocusAT1G26590
TemplateIT_06F06 (ABRC_145)
Donor VectorpDONR207
Forward primerIPF_06F06 (ggggacaagtttgtacaaaaaagcaggcttcATGGAGGAAGGGAAAGAGAATTCGTTT)
Reverse primerIPR_06F06 (ggggaccactttgtacaagaaagctgggttTTCATCAGTATCCGAAACAGGAAGATAGAG)
Alignment with template seq.1stアミノ酸配列100%一致2nd-
Alignment with TAIR7CDSアミノ酸配列100%一致-
comment
sequence 
>IE_06F06_1083bp
ATGGAGGAAGGGAAAGAGAATTCGTTTTTGTGCAAGTACTGTTACAAGACGTTTCCTTCTGGGAAAGCACTCGGTGGTCACATTAGAATTCATACGAACG
AGAATTCAGTTGGTTACAATGGCAATAAGAAGAAGAGGTTGGTGGATCAGAGAAAGATGATGGCTCAGAAACATAAGCAACAACAACAAGTTGGTTGCAG
AGAATGTGGTAGAGTGTTCGTTTCTTTGAAAGCTCTTAGAGGTCACATGGCTTGTCACGGTGAGGTAAAGAAGATGTTGATGGATGATAATAGCCAATCT
GATACTGAAAGTGAGACTTCCTCAGCTCCTATGAGGAAAAGATCCAAGAGGGTGGTCATGAAACAATCAAATTCTGAATCTTTGAGTAATGGGAGCTCGT
CTTTTGGTTCTGAGATTGACCAAGAACGTAGGGATGGGGCTTATAATTTGATGATGTTGTCGAGTGATTCTAGTAGTTTCAAGAAAAGACGTAACATGGT
GATGAATTCTCTTGGTGAGTCATCTGACAACAACTCTGCGATTGTTGAGAATAAGTCATCTTCAGGGGATGAGTTTAAGATCATGAATGTGAAGAATGCA
GTAGATGACCAATGGAGATCTGCTGATGATGATGGAGTTAGTCTCTGTGATTCAGATGGCTCTGATTCTGATTACTTCATGAATGGTCCAAGTAAGTCGG
AGTCAGACATTTCTGTTGACAGGAACACTGGATTTGGATCTGGATTCAACAACTCATTGATCAGGTTCAGGAATAGTAATGAGGGAGGAACAAAGTATGA
GCTGAGCAAAAGCAAGAGAGCCTTGTTTTCTTATGAAACTGATTCATGTGCAGACACAAACAGCAAGATTCATCATAGGTTCAAAGATAGCAAATCCTCA
ATGGTTAAGAAAGAAGGTGGTGAGAAGGTGAAGAGCAAAGGTCATGAGTGCCCAATTTGCTTCAGGATGTTCAAATCAGGCCAAGCTTTAGGTGGTCACA
AGAGATCACATTCCATTGCAAACCACGCAGCTGAAATGCGTAACCAAATCGATCTCTATCTTCCTGTTTCGGATACTGATGAA

>IE_06F06_translated
MEEGKENSFLCKYCYKTFPSGKALGGHIRIHTNENSVGYNGNKKKRLVDQRKMMAQKHKQQQQVGCRECGRVFVSLKALRGHMACHGEVKKMLMDDNSQS
DTESETSSAPMRKRSKRVVMKQSNSESLSNGSSSFGSEIDQERRDGAYNLMMLSSDSSSFKKRRNMVMNSLGESSDNNSAIVENKSSSGDEFKIMNVKNA
VDDQWRSADDDGVSLCDSDGSDSDYFMNGPSKSESDISVDRNTGFGSGFNNSLIRFRNSNEGGTKYELSKSKRALFSYETDSCADTNSKIHHRFKDSKSS
MVKKEGGEKVKSKGHECPICFRMFKSGQALGGHKRSHSIANHAAEMRNQIDLYLPVSDTDE
Alignment ◆CDS（TAIRver.9）_without_stop_codonとのalignment◆

100%一致しました。

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