| Code name | IE_06G08 | |||
|---|---|---|---|---|
| Locus | AT4G12350 | |||
| Template | IT_06G08 (ABRC_183) | |||
| Donor Vector | pDONR207 | |||
| Forward primer | IPF_06G08 (ggggacaagtttgtacaaaaaagcaggcttcATGGAAATCGATCCTTCGACCCATCAA) | |||
| Reverse primer | IPR_06G08 (ggggaccactttgtacaagaaagctgggttGTCCTTGTGACCCGTCTTCATGGAGCTTTG) | |||
| Alignment with template seq. | 1st | アミノ酸配列100%一致 | 2nd | - |
| Alignment with TAIR7CDS | アミノ酸配列100%一致 | - | ||
| comment | ||||
| sequence |
>IE_06G08_504bp ATGGAAATCGATCCTTCGACCCATCAACCTTTAAACAAAGTATTTACCGATACAAACTTAGTCGATAAATCTGAAACTTCATCGAAAGCCGACAATGTAA ATGATAATAAAATCGTAGAGATCGATGGGACAACGACAAATACAATAGATGATAGCATTATCACTCATCAAAATAGTTCAAATGATGATTATGAATTACT TGGTGATATAATTCATAATTATGGAGATTTATTTAATATTCTATGGACCAACGATGAACCTCCTCTAGTCGATGATGCATCATGGAGCAATCATAACGTT GGTATTGGAGGAACAGCTGCAGTTGCAGCCTCAGACAAGAACAACACTGCTGCCGAGGAAGATTTCCCGGAAAGATCATTTGAAAAACAGAACGGCGAAA GTTGGATGTTCTTGGATTATTGCCAAGAATTTGGTGTTGAAGATTTTGGGTTCGAGTGTTACCATGGTTTTGGTCAAAGCTCCATGAAGACGGGTCACAA GGAC >IE_06G08_translated MEIDPSTHQPLNKVFTDTNLVDKSETSSKADNVNDNKIVEIDGTTTNTIDDSIITHQNSSNDDYELLGDIIHNYGDLFNILWTNDEPPLVDDASWSNHNV GIGGTAAVAASDKNNTAAEEDFPERSFEKQNGESWMFLDYCQEFGVEDFGFECYHGFGQSSMKTGHKD |
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| Alignment |
◆CDS（TAIRver.9）_without_stop_codonとのalignment◆ 504/858 (58.7%)（アミノ酸配列で168/286 (58.7%)）一致しました。
10 20 30 40 50 60 70 80 90 100
AT4G12 ATGGGAAGGCAGCCATGTTGTGACAAGCTCATGGTGAAGAAGGGGCCGTGGACGGCGGAGGAAGACAAGAAACTGATAAACTTTATCTTGACCAACGGCC
IE_06G ----------------------------------------------------------------------------------------------------
110 120 130 140 150 160 170 180 190 200
AT4G12 ACTGTTGCTGGAGGGCTTTGCCGAAGCTGGCCGGTCTCCGTCGCTGTGGGAAGAGCTGCCGTCTACGGTGGACCAATTATCTCCGACCTGACTTGAAGAG
IE_06G ----------------------------------------------------------------------------------------------------
210 220 230 240 250 260 270 280 290 300
AT4G12 AGGTCTTCTCTCCGACGCCGAGGAACAGCTTGTCATCGACCTTCATGCTCTTCTCGGCAACAGATGGTCCAAGATCGCTGCAAGATTACCAGGAAGAACA
IE_06G ----------------------------------------------------------------------------------------------------
310 320 330 340 350 360 370 380 390 400
AT4G12 GACAACGAAATAAAAAATCATTGGAATACTCATATCAAGAAGAAGCTCCTTAAGATGGAAATCGATCCTTCGACCCATCAACCTTTAAACAAAGTATTTA
::::::::::::::::::::::::::::::::::::::::::::::
IE_06G ------------------------------------------------------ATGGAAATCGATCCTTCGACCCATCAACCTTTAAACAAAGTATTTA
10 20 30 40
410 420 430 440 450 460 470 480 490 500
AT4G12 CCGATACAAACTTAGTCGATAAATCTGAAACTTCATCGAAAGCCGACAATGTAAATGATAATAAAATCGTAGAGATCGATGGGACAACGACAAATACAAT
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_06G CCGATACAAACTTAGTCGATAAATCTGAAACTTCATCGAAAGCCGACAATGTAAATGATAATAAAATCGTAGAGATCGATGGGACAACGACAAATACAAT
50 60 70 80 90 100 110 120 130 140
510 520 530 540 550 560 570 580 590 600
AT4G12 AGATGATAGCATTATCACTCATCAAAATAGTTCAAATGATGATTATGAATTACTTGGTGATATAATTCATAATTATGGAGATTTATTTAATATTCTATGG
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_06G AGATGATAGCATTATCACTCATCAAAATAGTTCAAATGATGATTATGAATTACTTGGTGATATAATTCATAATTATGGAGATTTATTTAATATTCTATGG
150 160 170 180 190 200 210 220 230 240
610 620 630 640 650 660 670 680 690 700
AT4G12 ACCAACGATGAACCTCCTCTAGTCGATGATGCATCATGGAGCAATCATAACGTTGGTATTGGAGGAACAGCTGCAGTTGCAGCCTCAGACAAGAACAACA
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_06G ACCAACGATGAACCTCCTCTAGTCGATGATGCATCATGGAGCAATCATAACGTTGGTATTGGAGGAACAGCTGCAGTTGCAGCCTCAGACAAGAACAACA
250 260 270 280 290 300 310 320 330 340
710 720 730 740 750 760 770 780 790 800
AT4G12 CTGCTGCCGAGGAAGATTTCCCGGAAAGATCATTTGAAAAACAGAACGGCGAAAGTTGGATGTTCTTGGATTATTGCCAAGAATTTGGTGTTGAAGATTT
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_06G CTGCTGCCGAGGAAGATTTCCCGGAAAGATCATTTGAAAAACAGAACGGCGAAAGTTGGATGTTCTTGGATTATTGCCAAGAATTTGGTGTTGAAGATTT
350 360 370 380 390 400 410 420 430 440
810 820 830 840 850
AT4G12 TGGGTTCGAGTGTTACCATGGTTTTGGTCAAAGCTCCATGAAGACGGGTCACAAGGAC
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_06G TGGGTTCGAGTGTTACCATGGTTTTGGTCAAAGCTCCATGAAGACGGGTCACAAGGAC
450 460 470 480 490 500
10 20 30 40 50 60 70 80 90 100
AT4G12 MGRQPCCDKLMVKKGPWTAEEDKKLINFILTNGHCCWRALPKLAGLRRCGKSCRLRWTNYLRPDLKRGLLSDAEEQLVIDLHALLGNRWSKIAARLPGRT
IE_06G ----------------------------------------------------------------------------------------------------
110 120 130 140 150 160 170 180 190 200
AT4G12 DNEIKNHWNTHIKKKLLKMEIDPSTHQPLNKVFTDTNLVDKSETSSKADNVNDNKIVEIDGTTTNTIDDSIITHQNSSNDDYELLGDIIHNYGDLFNILW
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_06G ------------------MEIDPSTHQPLNKVFTDTNLVDKSETSSKADNVNDNKIVEIDGTTTNTIDDSIITHQNSSNDDYELLGDIIHNYGDLFNILW
10 20 30 40 50 60 70 80
210 220 230 240 250 260 270 280
AT4G12 TNDEPPLVDDASWSNHNVGIGGTAAVAASDKNNTAAEEDFPERSFEKQNGESWMFLDYCQEFGVEDFGFECYHGFGQSSMKTGHKD
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
IE_06G TNDEPPLVDDASWSNHNVGIGGTAAVAASDKNNTAAEEDFPERSFEKQNGESWMFLDYCQEFGVEDFGFECYHGFGQSSMKTGHKD
90 100 110 120 130 140 150 160
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