◆◆◆IE_06H04（IE_09C08） detail◆◆◆

Code nameIE_06H04（IE_09C08）
LocusAT5G22990
TemplateIT_06H04 (ABRC_206)
Donor VectorpDONR207
Forward primerIPF_06H04 (ggggacaagtttgtacaaaaaagcaggcttcATGACTTCAATCCCAAATGGCTTAAAT)
Reverse primerIPR_06H04 (ggggaccactttgtacaagaaagctgggttCTTGCTACTTGCTATGTTGTTATCGCCTCC)
Alignment with template seq.1stアミノ酸配列99.70%一致2ndアミノ酸配列100%一致
Alignment with TAIR7CDSアミノ酸配列99.70%一致アミノ酸配列100%一致
comment1st:(Sequence解析まで行ったが、フレームはずれないが塩基置換がある)→2nd(09C08):100%
sequence 
>IE_06H04_972bp
ATGACTTCAATCCCAAATGGCTTAAATTCTTATGTTGACGATACTGTGAACATTTGTGGTTTTACTCCCATCGAAATGAGTTCCAACCTTAGGAATCATG
AGTCTAAGATGGTTCACTCAATGTTCAATACTTCTGACCACACGAATCATCATGGTTTGTTTTCTTCCTCTCGTGTGTTTAATTTTTATCAAGATTCACA
TGTTTCTTCATCTTCTTTTGGTTTCAACAATTCACATATGGCTTATCATATGAGAAAAAACATGGTTTCCACTTTTGGGATGCCTTGCATAACTCAAAAC
TCAAATAACCCTCATCTTTCTCAAATCTCTATTACTCAAACTATCACAAATAGCTATTCTGCTATTGTTCCTACCTATAATCTTATTACTTCCCAAAATG
AATATCAACGTGCCAAAGAACCCAACATCTTCAATCCTCCTTTTTACCCTCCAAATTTTGTTGACAAAAATGTTGGAAACCAATGCCAGATCTTGAATCC
TACACCCCTCAATACCATTTTTCCACATCAAGCTTCCATATTTCCTAGGAATGTAGACAAATTTTCTTTTTCACCAAAACAGAACCCTCACCAATATGTT
TCGTATCGCCAACCCCTGAAAAGACATTGTAGACCAACCAAAAAATTTGAAAATACTTTTAGTGATTTTGATTCTGGAAAAGATATTGAATACGATGGTC
GGACGCATAGCTTACCATATGAGAAATACGGTCCATATACATGTCCCAAATGTAACAGTGTGTTTGACACTTCACAAAAGTTTGCTGCACATATATCATC
GATGCACTACAAAAATGAGTCCATAGAAGAGAAATTTAAGAGATATAATGCAAGGAACAAAAAGAGATTACGTAAAAGAAACCAAATGATCCATGAAGAA
TCTAACAATATCCAACCAGAAGAGATAGTTGCAGAGGAAAGCGGAGGCGATAACAACATAGCAAGTAGCAAG

>IE_06H04_translated
MTSIPNGLNSYVDDTVNICGFTPIEMSSNLRNHESKMVHSMFNTSDHTNHHGLFSSSRVFNFYQDSHVSSSSFGFNNSHMAYHMRKNMVSTFGMPCITQN
SNNPHLSQISITQTITNSYSAIVPTYNLITSQNEYQRAKEPNIFNPPFYPPNFVDKNVGNQCQILNPTPLNTIFPHQASIFPRNVDKFSFSPKQNPHQYV
SYRQPLKRHCRPTKKFENTFSDFDSGKDIEYDGRTHSLPYEKYGPYTCPKCNSVFDTSQKFAAHISSMHYKNESIEEKFKRYNARNKKRLRKRNQMIHEE
SNNIQPEEIVAEESGGDNNIASSK
Alignment ◆CDS（TAIRver.9）_without_stop_codonとのalignment◆

100%一致しました。

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